Figures & data
Figure 1. (a) The formation of polyplexes by ionic interaction between negatively charged DNA and polycations (b) major barriers that polyplexes should overcome for efficient gene delivery Extracellular barriers include the maintenance of stability of the polyplexes during systemic circulation, and the transport from blood vessels to the tissue. Intracellular barriers for delivery include (i) cellular uptake via endocytosis, (ii) endosomal escape, (iii) transport to the perinuclear space, (iv) unpacking of the polyplexes, and (v) nuclear translocation (Molecular Design of Functional Polymers for Gene Therapy, 2007).
Figure 2. Electrophoresis for DNA concentration with polymer (DNA–Polymer Complexes for Gene Therapy, 2012).
Figure 3. Representation of a fourth generation dendrimer.
