Figures & data
Figure 1. IL7 Ligand generates increased CD26 + cells from cord blood mononuclear cells. 105 cord blood mononuclear cells cultured on 96 well plate by using different combination of cytokines and CD26 + cells analyzed by FACS in day 7, 14, 21. (A) isotype control sample. (B) Representative FACS profiles from mononuclear cord blood cultured cells. (C) Mean (SD) proportion of CD26 + cells after 14 days; p < 0.001.
Figure 2. Cytokines can efficiently produce CD26 + cells. 105 cord blood mononuclear cells cultured with different combination of cytokines. Cultured cells harvested in identical time points and analyzed by FACS. Mean proportion of CD26 + cells have shown with combination of SCF+ FL+ IL2 + IL7+ IL15; p < 0.0001 (A), SCF+ FL; p < 0.0007 (B), SCF+ FL+ IL2; no significant (C), SCF+ FL+ IL15; p < 0.0001 (D) and SCF+ FL+ IL7; p < 0.01 (E).
Figure 3. Mean fluorescence intensity (MFI) of different cytokines are in same level. Mean fluorescence intensity of CD26 + cell evaluated by FACS in indicated time points from CD26 + cell-derived cord blood mononuclear cells; p < 0.0001.
Figure 4. Representative FACS profile of 105 cultured cord blood mononuclear cells in identical time points. CD34+ CD26+ and CD34+CD26− evaluated by gating on lymphoid population in FSC versus SSC.
Figure 5. Evaluation of CD34+ CD26+and CD34+CD26− cells derived from cord blood mononuclear cells at different time points. The harvested cells evaluated by FACS and mean (SD) have shown using no cytokines (A), SCF+ FL+ IL2 + IL7+ IL15 (B), SCF+ FL (C), SCF+ FL+ IL2 (D), SCF+ FL+ IL7 (E) and SCF+ FL+ IL15 (F).
