Effect of MT1-MMP Deficiency and Overexpression in Corneal Keratocytes on Vascular Endothelial Cell Migration and Proliferation

Abstract

Purpose: To determine the effect of keratocyte-derived MT1-MMP on calf pulmonary artery endothelial cell (CPAE) proliferation and migration. Methods: Keratocyte lines were generated from MT1-MMP knockout (KO) and wild type (WT) mice. WT keratocytes were transfected with WT or mutant MT1-MMP DNAs (ΔTC or E240A). The effect of keratocyte-conditioned media on CPAE proliferation and migration was assayed. Results: KO keratocyte conditioned media resulted in the greatest increase of CPAE cell proliferation (190.5 ± 6.0%; p < 0.01). WT keratocyte conditioned media showed higher CPAE proliferation (155.4 ± 3.6%) than WT/MT1-MMP-transfected keratocytes (119.7 ± 2.2%; p < 0.001). Migration assays confirmed these findings. Conclusions: Keratocyte-derived MT1-MMP has anti-angiogenic effects in CPAE cells.

Keywords:

• angiogenesis
• gene/expression
• keratocyte
• keratocyte-derived MT1-MMP
• metalloproteinases
• MT1-MMP

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Correction to: Effect of MT1-MMP deficiency and overexpression in corneal keratocytes on vascular endothelial cell migration and proliferation