Brilliant green decolorization by dye decolorizing peroxidase producing bacterium, Achromobacter insolitus isolated from Forest soils of Similipal Biosphere Reserve, Odisha

Abstract

Dye-decolorizing peroxidases (DyPs) are recently identified superfamily of heme-containing peroxidases which has received much attention for its potential application in dye decolourization. In the current research, six bacterial colonies (SDB1-6) showing lignolytic activity were isolated from forest soils of SBR using a minimal salt medium supplemented with alkali lignin and screened for DyP activity using brilliant green dye supplemented luria bertani agar medium. The highest DyP activity showing bacterium was identified using biochemical and 16SrRNA sequencing and was identified as Achromobacter insolitus. A. insolitus was found to be an efficient strain showing the highest DyP activity of 2.7 U/mL/ml at un-optimized condition. The RSM optimization was conducted to increase the DyP enzyme activity. A. insolitus demonstrated DyP activity of 6.20 U/mL/min with an increase of 2.29 times of enzyme activity in optimal condition of pH 7.0, temperature 25 °C, H₂O₂ concentration 0.1 mM, and time 24 h. The bacterium was further screened for dye decolorization taking crystal violet, methyl orange, methylene blue, brilliant green and methyl red dyes supplemented in LB medium. The bacterium showed maximum brilliant green degradation ability was analyzed using UV-Vis and FTIR analyses to characterize the degraded product. The highest brilliant green degradation activity of the A. insolitus in the present study could be exploited for bioremediation of brilliant green contaminated industrial effluents in a ecofriendly and cost effective way.

Graphical Abstract

Keywords:

• Bioremediation
• dye decolorizing peroxidase
• ligninolytic bacteria
• response surface methodology

Acknowledgments

The authors extend their thanks to the Head of the Department of Biotechnology at Maharaja Sriram Chandra Bhanja Deo University, Baripada, Odisha, India, for supplying the necessary infrastructure facilities for the ongoing study. Furthermore, the authors express their gratitude to the Department of Applied Geology (AGL), IIT (ISM) Dhanbad (http://www.iitism.ac.in), for performing FTIR analyses and supplying visualizations for this research. The authors are also appreciative of AGL, IIT (ISM), Dhanbad, for allowing access to equipment, providing technical support, and offering laboratory facilities through the DST-FIST Level-II Program [No. SR/FST/ESII-014/2012(C)].

Authors’ contributions

SD and SR wrote all the parts of this research paper with related previous review works, and contributed original manuscript and formatting. SD and SR conducted major part of experiments, investigated the results, analyzed data, validated the results with related previous works. Whereas, AS helped in doing some of experiments like biochemical analysis. HT supervised the whole work, conceptualized the research, and guided the writing & editing of the manuscript. All authors have read and approved the manuscript

Consent to participate

Not applicable.

Consent to publish

Not applicable.

Disclosure statement

No potential conflict of interest was reported by the author(s).

Ethical approval

Not applicable.

Data availability statement

All data generated or analyzed during this study are included in this article.

Additional information

Funding

This work was financial supporter by Biju Patnaik Research fellowship, Govt. of Odisha (ST- BT-MISC-007-2020/2047) to the author (SR) for conducting her research work.