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Journal of Immunoassay and Immunochemistry Volume 37, 2016 - Issue 1
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Original Articles

Anti-Inflammatory Activity in the Low Molecular Weight Fraction of Commercial Human Serum Albumin (LMWF5A)

Gregory W. ThomasSwedish Medical Center, Trauma Research Department, Englewood, Colorado, USA;St. Anthony Hospital, Lakewood, Colorado, USA;The Medical Center of Plano, Plano, Texas, USAView further author information
,
Leonard T. RaelSwedish Medical Center, Trauma Research Department, Englewood, Colorado, USA;St. Anthony Hospital, Lakewood, Colorado, USA;The Medical Center of Plano, Plano, Texas, USAView further author information
,
Charles W. MainsSt. Anthony Hospital, Lakewood, Colorado, USA;Rocky Vista University, Parker, Colorado, USAView further author information
,
Denetta SloneSwedish Medical Center, Trauma Research Department, Englewood, Colorado, USA;Rocky Vista University, Parker, Colorado, USAView further author information
,
Matthew M. CarrickThe Medical Center of Plano, Plano, Texas, USAView further author information
,
Raphael Bar-OrSwedish Medical Center, Trauma Research Department, Englewood, Colorado, USA;St. Anthony Hospital, Lakewood, Colorado, USA;The Medical Center of Plano, Plano, Texas, USAView further author information
&
David Bar-OrSwedish Medical Center, Trauma Research Department, Englewood, Colorado, USA;St. Anthony Hospital, Lakewood, Colorado, USA;The Medical Center of Plano, Plano, Texas, USA;Rocky Vista University, Parker, Colorado, USACorrespondence[email protected]
View further author information
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Pages 55-67 | Published online: 12 Jan 2016
 
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Abstract

The innate immune system is increasingly being recognized as a critical component in osteoarthritis (OA) pathophysiology. An ex vivo immunoassay utilizing human peripheral blood mononuclear cells (PBMC) was developed in order to assess the OA anti-inflammatory properties of the low molecular weight fraction (<5 kDa) of commercial human serum albumin (LMWF5A). PBMC from various donors were pre-incubated with LMWF5A before LPS stimulation. TNFα release was measured by ELISA in supernatants after an overnight incubation. A ≥ 30% decrease in TNFα release was observed. This anti-inflammatory effect is potentially useful in assessing potency of LMWF5A for the treatment of OA.

Additional information

Funding

All support and funding for this study was provided by Ampio Pharmaceuticals, Inc. (Englewood, CO, USA).

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