Transcreener™: screening enzymes involved in covalent regulation

Abstract

Enzymes that catalyse group transfer reactions comprise a significant fraction of the human proteome and are a rich source of drug targets because of their role in covalent regulatory cycles. Phosphorylation, glycosylation, sulfonation, methylation and acetylation represent some of the key types of group transfer reactions that modulate the function of diverse biomolecules through covalent modification. Development of high-throughput screening methods for these enzymes has been problematic because of the diversity of acceptor substrates. Recently, the authors developed a novel assay platform called Transcreener™ that relies upon fluorescence detection of the invariant reaction product of a group transfer reaction, usually a nucleotide. This platform enables screening of any isoform in a family of group transfer enzymes, with any acceptor substrate, using the same assay reagents.

Keywords::

• covalent modification
• fluorescence polarisation
• glycosylation
• glycosyltransferase
• high-throughput screening (HTS)
• phosphorylation
• protein kinase
• sulfation
• sulfonation
• sulfotransferase