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Cancer Investigation Volume 28, 2010 - Issue 5
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ORIGINAL ARTICLEClinical Translational Therapeutics

Adeno-Associated Virus Harboring Fusion Gene NT4-Ant-Shepherdin Induce Cell Death in Human Lung Cancer Cells

Tang XiaojiangDepartment of Oncosurgery, First Affiliated Hospital, Department of Anatomy, Histology & Embryology, Department of Geratic Surgery, First Affiliated Hospital, Medical School of Xi'an Jiaotong University, Xi'an 710061, China, and Xi'an Huaguang Bio-engineering Company, Xi'an 710025, China
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Zhou JinsongDepartment of Oncosurgery, First Affiliated Hospital, Department of Anatomy, Histology & Embryology, Department of Geratic Surgery, First Affiliated Hospital, Medical School of Xi'an Jiaotong University, Xi'an 710061, China, and Xi'an Huaguang Bio-engineering Company, Xi'an 710025, China
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Wang JianshengDepartment of Oncosurgery, First Affiliated Hospital, Department of Anatomy, Histology & Embryology, Department of Geratic Surgery, First Affiliated Hospital, Medical School of Xi'an Jiaotong University, Xi'an 710061, China, and Xi'an Huaguang Bio-engineering Company, Xi'an 710025, China
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Pan ChengenDepartment of Oncosurgery, First Affiliated Hospital, Department of Anatomy, Histology & Embryology, Department of Geratic Surgery, First Affiliated Hospital, Medical School of Xi'an Jiaotong University, Xi'an 710061, China, and Xi'an Huaguang Bio-engineering Company, Xi'an 710025, China
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Yang GuangxiaoDepartment of Oncosurgery, First Affiliated Hospital, Department of Anatomy, Histology & Embryology, Department of Geratic Surgery, First Affiliated Hospital, Medical School of Xi'an Jiaotong University, Xi'an 710061, China, and Xi'an Huaguang Bio-engineering Company, Xi'an 710025, China
&
Wang QuanyingDepartment of Oncosurgery, First Affiliated Hospital, Department of Anatomy, Histology & Embryology, Department of Geratic Surgery, First Affiliated Hospital, Medical School of Xi'an Jiaotong University, Xi'an 710061, China, and Xi'an Huaguang Bio-engineering Company, Xi'an 710025, China
Pages 465-471 | Published online: 07 Dec 2009
 
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ABSTRACT

To further enhance anticancer effect of Shepherdin and overcome limitation of peptide therapy, recombinant adeno-associated virus (rAAV) was constructed with following strategies: therapeutic peptide secretory expression and adeno-associated virus gene transfer system. MTT assay and flow cytometric analysis revealed that rAAV harboring fusion gene NT4-Ant-Shepherdin significantly suppressed A549 cell growth in a time-dependent manner and induced apoptosis. In the infected A549 cells, survivin expression level decreased strongly while Caspase-3/7 activities increased significantly. These results indicated that rAAV harboring fusion gene NT4-Ant-Shepherdin may be a novel strategy in lung cancer peptide therapy.

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