Paradoxical Masking Effects of Bright Photophase and High Temperature in Drosophila malerkotliana

Abstract

Synergic contribution of light and temperature is known to cause a paradoxical masking effect (inhibition of activity by bright light and high temperature) on various rhythms of animals. The present study reports the paradoxical masking effects of 1000-lux photophase at 25°C on the locomotor activity rhythm of Drosophila malerkotliana. Flies were subjected to light (L)-dark (D) 12:12 cycles wherein the photophase was varied from 10 to 1000 lux, whereas the scotophase was set to 0 lux in these and subsequent LD cycles. At 10, 100, and 500 lux, the flies were diurnal; however, at 1000 lux they were nocturnal. Transfer from LD 12:12 cycles to continuous darkness (DD) initiated free-running rhythmicity in all flies. Free-running rhythms of the flies switched from the 10-lux to the 500-lux groups started from the last activity-onset phase of the rhythm following 3–5 transient cycles, suggesting involvement of the circadian pacemaker. In contrast, the free-running rhythm of the flies of the 1000-lux group began abruptly from the last lights-on phase of the LD cycle, indicating noninvolvement of the pacemaker. Furthermore, all flies showed nocturnal activity in the two types of LD 12:12 cycles when the photophase was 1000 lux. The first type of LD cycles had three succeeding photophases of 100, 1000, and again 100 lux, whereas the second type of LD cycles had only one photophase of 1000 lux, but the LD 12:12 cycles were reversed to DL 12:12 cycles. Apparently, the combined effects of light and temperature caused such paradoxical masking effects. This hypothesis was tested by repeating the above experiments at 20°C. Flies in all experiments exhibited a diurnal activity pattern, even when the photophase was 1000 lux. Thus, the present study demonstrates that the paradoxical masking effect in D. malerkotliana was caused by the additive influence of light intensity and temperature. This strategy appears to have physiological significance, i.e., to shun and thus protect against the bright photophase at high temperature in the field. (Author correspondence: [email protected])

Keywords:

• Drosophila
• Entrainment
• Light
• Paradoxical masking
• Temperature
• Zebra finch

ACKNOWLEDGMENTS

The study was supported by the research grant no. SP/SO/AS–43/2004 and FIST SR/SO/AS-MS01/2009 funded by Department of Science and Technology, New Delhi, to D.S.J. The authors thank three anonymous reviewers for their valuable comments on an earlier version of the manuscript.

Declaration of Interest: The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.