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TECHNICAL NOTE

The line blot assay: Problems with titrating first and second antibodies for Western blot and immunohistochemistry assays?

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Pages 357-362 | Received 17 Oct 2005, Accepted 22 Feb 2006, Published online: 08 Jul 2009

References

  • Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 1979; 76: 4350–4
  • Towbin H., Gordon J. Immunoblotting and dot immunoblotting: current status and outlook. J Immunol Meth 1984; 72: 313–40
  • Lyashchenko K. P., Singh M., Colangeli B., Gennaro M. L. A multi‐antigen print immunoassay for the serological diagnosis of infectious diseases. J Immunol Methods 2000; 242: 91–100

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