217
Views
9
CrossRef citations to date
0
Altmetric
Original Article

Epidemiological typing of methicillin-resistant Staphylococcus aureus (MRSA): spa typing versus pulsed-field gel electrophoresis

, , , , , , , , , & show all
Pages 433-439 | Received 14 Sep 2008, Published online: 21 Jul 2009

References

  • Engemann JJ, Carmeli Y, Cosgrove SE, Fowler VG, Bronstein MZ, Trivette SL, et al. Adverse clinical and economic outcomes attributable to methicillin resistance among patients with Staphylococcus aureus surgical site infection. Clin Infect Dis 2003; 36: 592–8
  • Crum NF, Lee RU, Thornton SA, Stine OC, Wallace MR, Barrozo C, et al. 15-y study of the changing epidemiology of methicillin-resistant Staphylococcus aureus. Am J Med 2006; 119: 943–51
  • Asensio A, Canton R, Vaque J, Rossello J, Calbo F, Garcia-Caballero J, et al. Nosocomial and community acquired methicillin-resistant Staphylococcus aureus infections in hospitalized patients (Spain, 1993–2003). J Hosp Infect 2006; 63: 465–71
  • Jung SI, Shin DH, Park KH, Shin JH. Antimicrobial susceptibility and clonal relatedness between community- and hospital-acquired methicillin-resistant Staphylococcus aureus from blood cultures. J Microbiol 2006; 44: 336–43
  • Faria NA, Oliveira DC, Westh H, Monnet DL, Larsen AR, Skov R, et al. Epidemiology of emerging methicillin-resistant Staphylococcus aureus (MRSA) in Denmark: a nationwide study in a country with low prevalence of MRSA infection. J Clin Microbiol 2005; 43: 1836–42
  • Cunningham R, Jenks P, Northwood J, Wallis M, Ferguson S, Hunt S. Effect on MRSA transmission of rapid PCR testing of patients admitted to critical care. J Hosp Infect 2007; 65: 24–8
  • Schlichting C, Branger C, Fournier JM, Witte W, Boutonnier A, Wolz C, et al. Typing of Staphylococcus aureus by pulsed-field gel electrophoresis, zymotyping, capsular typing, and phage typing: resolution of clonal relationships. J Clin Microbiol 1993; 31: 227–32
  • Carles-Nurit MJ, Christophle B, Broche S, Gouby A, Bouziges N, Ramuz M. DNA polymorphisms in methicillin-susceptible and methicillin-resistant strains of Staphylococcus aureus. J Clin Microbiol 1992; 30: 2092–6
  • Murchan S, Kaufmann ME, Deplano A, de Ryck R, Struelens M, Zinn CE, et al. Harmonization of pulsed-field gel electrophoresis protocols for epidemiological typing of strains of methicillin-resistant Staphylococcus aureus: a single approach developed by consensus in 10 European laboratories and its application for tracing the spread of related strains. J Clin Microbiol 2003; 41: 1574–85
  • Mulvey MR, Chui L, Ismail J, Louie L, Murphy C, Chang N, et al. Development of a Canadian standardized protocol for subtyping methicillin-resistant Staphylococcus aureus using pulsed-field gel electrophoresis. J Clin Microbiol 2001; 39: 3481–5
  • Harmsen D, Claus H, Witte W, Rothganger J, Claus H, Turnwald D, et al. Typing of methicillin-resistant Staphylococcus aureus in a university hospital setting by using novel software for spa repeat determination and database management. J Clin Microbiol 2003; 41: 5442–8
  • Matussek A, Taipalensuu J, Einemo IM, Tiefenthal M, Lofgren S. Transmission of Staphylococcus aureus from maternity unit staff members to newborns disclosed through spa typing. Am J Infect Control 2007; 35: 122–5
  • Shopsin B, Gomez M, Montgomery SO, Smith DH, Waddington M, Dodge DE, et al. Evaluation of protein A gene polymorphic region DNA sequencing for typing of Staphylococcus aureus strains. J Clin Microbiol 1999; 37: 3556–63
  • Tang YW, Waddington MG, Smith DH, Manahan JM, Kohner PC, Highsmith LM, et al. Comparison of protein A gene sequencing with pulsed-field gel electrophoresis and epidemiological data for molecular typing of methicillin-resistant Staphylococcus aureus. J Clin Microbiol 2000; 38: 1347–51
  • Nilsson P, Alexandersson H, Ripa T. Use of broth enrichment and real-time PCR to exclude the presence of methicillin-resistant Staphylococcus aureus in clinical samples: a sensitive screening approach. Clin Microbiol Infect 2005; 11: 1027–34
  • Maslow J, Slutsky A, Arbeit R. Application of pulsed-field gel electrophoresis to molecular epidemiology. Diagnostic molecular microbiology: principles and applications, D Persing, T Smith, F Tenover, T White. ASM Press, Washington, DC 1993; 563–72
  • Lina G, Piemont Y, Godail-Gamot F, Bes M, Peter MO, Gauduchon V, et al. Involvement of Panton-Valentine leukocidin-producing Staphylococcus aureus in primary skin infections and pneumonia. Clin Infect Dis 1999; 29: 1128–32
  • Enright MC, Day NP, Davies CE, Peacock SJ, Spratt BG. Multilocus sequence typing for characterization of methicillin-resistant and methicillin-susceptible clones of Staphylococcus aureus. J Clin Microbiol 2000; 38: 1008–15
  • Hunter PR, Gaston MA. Numerical index of the discriminatory ability of typing systems: an application of Simpson's index of diversity. J Clin Microbiol 1988; 26: 2465–6
  • Rabkin CS, Jarvis WR, Anderson RL, Govan J, Klinger J, LiPuma J, et al. Pseudomonas cepacia typing systems: collaborative study to assess their potential in epidemiologic investigations. Rev Infect Dis 1989; 11: 600–7
  • van Belkum A, Tassios PT, Dijkshoorn L, Haeggman S, Cookson B, Fry NK, et al. Guidelines for the validation and application of typing methods for use in bacterial epidemiology. Clin Microbiol Infect 2007; 13(Suppl 3)1–46
  • Mellmann A, Friedrich AW, Rosenkotter N, Rothganger J, Karch H, Reintjes R, et al. Automated DNA sequence-based early warning system for the detection of methicillin-resistant Staphylococcus aureus outbreaks. PLoS Med 2006; 3: 33
  • Hallin M, Deplano A, Denis O, de Mendonca R, de Ryck R, Struelens MJ. Validation of pulsed-field gel electrophoresis and spa typing for long-term, nationwide epidemiological surveillance studies of Staphylococcus aureus infections. J Clin Microbiol 2007; 45: 127–33
  • Kahl BC, Mellmann A, Deiwick S, Peters G, Harmsen D. Variation of the polymorphic region X of the protein A gene during persistent airway infection of cystic fibrosis patients reflects 2 independent mechanisms of genetic change in Staphylococcus aureus. J Clin Microbiol 2005; 43: 502–5
  • Frenay HM, Bunschoten AE, Schouls LM, van Leeuwen WJ, van den Broucke-Grauls CM, Verhoef J, et al. Molecular typing of methicillin-resistant Staphylococcus aureus on the basis of protein A gene polymorphism. Eur J Clin Microbiol Infect Dis 1996; 15: 60–4
  • Seybold U, Kourbatova EV, Johnson JG, Halvosa SJ, Wang YF, King MD, et al. Emergence of community-associated methicillin-resistant Staphylococcus aureus USA300 genotype as a major cause of healthcare-associated bloodstream infections. Clin Infect Dis 2006; 42: 647–56

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.