Publication Cover
Xenobiotica
the fate of foreign compounds in biological systems
Volume 30, 2000 - Issue 1
142
Views
31
CrossRef citations to date
0
Altmetric
Research Article

Cytochrome P450 enzyme activity and protein expression in primary porcine enterocyte and hepatocyte cultures

Pages 27-46 | Published online: 27 Aug 2009

References

  • ADAMS, J. C. and WATT, F. M., 1993, Regulation of development and differentiation by the extracellular matrix. Development, 117, 1183–1198.
  • ALTHAUS, F. R., SINCIAIR, J. F., SINCIAIR, P. and MEYERS, U. A., 1979, Drug-mediated induction of cytochromes P-450 and drug metabolism in cultured hepatocytes maintained in chemically defined medium. Journal of Biological Chemistry, 254, 2148–2153.
  • BAILEY, D. G., ARNOLD, J. M. and SPENCE, J. D., 1994, Grapefruit juice and drugs. How significant is the interaction? Clinical Pharmacokinetics, 26, 91–98.
  • BORLAK, J. T., SCOTT, A., HENDERSON, C. J., JENICE, H. J. and WOLF, C. R., 1996, Transfer of PCB's via lactation simultaneously induces the expression of P450 isoenzymes and the protooncogenes c-Ha-ras and c-raf in neonates. Biochemical Pharmacology, 51, 517–529.
  • CAIRNE, A. B., LAMERTON, L. F. and STEEL, G. G., 1965, Cell proliferation studies in the intestinal epithelium of the rat. I: Determination of the kinetic parameters. Experimental Cell Research, 39, 528–538.
  • CALDWELL, J., 1992, Problems and opportunities in toxicity testing arising from species differences in xenobiotic metabolism. Toxicology Letters, 64/65, 651–659.
  • DUNN, J. C., TOMPICINS, R. G. and YARMUSH, M. L., 1992, Hepatocytes in collagen sandwich: evidence for transcriptional and translational regulation. Journal of Cell Biology, 116, 1043–1053.
  • ELSDALE, T. and BARD, J., 1972, Collagen substrata for studies on cell behavior. Journal of Cell Biology, 54, 626–637.
  • EVANS, G. S., FuNr, N., SOMERS, A. S., EYDEN, B. and POTTEN, C. S., 1992, The development of a method for the preparation of rat intestinal epithelial cell primary cultures. Journal of Cell Science, 101, 219–231.
  • FrrzsimmoNs, M. E. and COLLINS, J. M. 1997, Selective biotransformation of the human immuno-deficiency virus protease inhibitor saquinavir by human small-intestinal cytochrome P4503A4: potential contribution to high first-pass metabolism. Drug Metabolism and Disposition, 25, 256–266.
  • FORSTER, U., LUIPPOLD, G. and SCHWARZ, L. R., 1986, Induction of monooxygenase and UDP-glucuronosyltransfera activities in primary cultures of rat hepatocytes. Drug Metabolism and Disposition, 14, 353–360.
  • FRANICE, W. W., APPELHANS, B., SCHMID, E., FREUDENSTEIN C., OSBORN, M. and WEBER, K., 1979, The organization of cytokeratin filaments in the intestinal epithelium. European Journal of Cell Biology, 19, 255–268.
  • GAN, L. S., MOSELEY, M. A., KHOSLA, B., AUGUSTUNS, P. F., BRADSHAW, T. P., HENDREN, R. W. and THAKICER, D. R., 1996, CYP3A-like cytochrome P450-mediated metabolism and polarized efflux of cyclosporin A in Caco-2 cells. Drug Metabolism and Disposition, 24, 344–349.
  • GIBSON, P. R., VAN DE POL, E., MAXWELL, L. E., GABRIEL, A. and DOE, W. F., 1989, Isolation of colonic crypts that maintain structural and metabolic viability in vitro. Gastroenterology, 96, 283–291.
  • GOLDSTEIN, J. A., IsHizmu, T., CHIBA, K., DE MORAIS S. M., BELL, D., KRAHN, P. M. and EVANS, D. A., 1997, Frequencies of the defective CYP2C19 alleles responsible for the mephenytoin poor metabolizer phenotype in various Oriental, Caucasian, Saudi Arabian and American black populations. Pharmacogenetics, 7, 59–64.
  • GORDON, J. I., 1989, Intestinal epithelial differentiation: new insights from chimeric and transgenic mice. Journal of Cell Biology, 108, 1187–1194.
  • GUENGERICH, F., 1982, Microsomal enzymes involved in toxicology—analysis and separation. In A. Hayes (ed.), Principles and Methods of Toxicology (New York: Raven), pp. 609–637.
  • HAHN, U., STALLMACH, A. and RIECKEN, E. 0., 1990, Basement membrane components are potent promotors of rat intestinal epithelial cell differentiation in vitro. Gastroenterology, 98, 322–335.
  • HAHN, U., CH0, A., SCHUPPAN, D., HAHN, E.G., MERE, H. J. and RIECKEN, E. 0., 1987, Intestinal epithelial cells preferentially attach to a biomatrix derived from human intestinal mucosa. Gut, 28 (Si), 153–158.
  • HEBERT, M. F., ROBERTS, J. P., PRUEKSARITANONT, T. and BENET, L. Z., 1992, Bioavailability of cyclosporine with concomitant rifampin administration is markedly less than predicted by hepatic enzyme induction. Clinical Pharmacology and Therapeutics, 52, 453–437.
  • HOUSTON, J. B., 1994, Utility of in vitro drug metabolism data in predicting in vivo metabolic clearance. Biochemical Pharmacology, 47, 1469–1479.
  • HUMPHREY, M. J. and Swum, D. A., 1992, Role of metabolism and pharmacokinetic studies in the discovery of new drugs—present and future perspectives. Xenobiotica, 22, 743–755.
  • JUNG, F., RICHARDSON, T. H., RAUCY, J. L. and JOHNSON, E. F., 1997, Diazepam metabolism by cDNA-expressed human 2C P450: identification of P4502C18 and P4502C19 as low K. diazepam N-demethylases. Drug Metabolism and Disposition, 25, 133–139.
  • KAIVINSKY, L. S. and FASCO, M. J., 1991, Small intestinal cytochromes P450. Critical Reviews in Toxicology, 21, 407–422.
  • KERN, A., BADE, A., PICHLMAYR, R. and SEWING, K. F., 1997, Drug metabolism in hepatocyte sandwich cultures of rats and humans. Biochemical Pharmacology, 54, 761–772.
  • KIVIST 0, K. T., BOOKJANS, G., FROMM, M. F., GRIESE, E. U., MUNZEL, P. and KROEMER, H. K., 1996, Expression of CYP3A4, CYP3A5 and CYP3A7 in human duodenal tissue. British Journal of Clinical Pharmacology, 42, 387–389.
  • KOIARS, J. C., AWNI, W. M., MERION, R. M. and WATKINS, P. B., 1991, First pass metabolism of cyclosporine by the gut. Lancet, 338, 1488–1490.
  • KOLARS, J. C., LOWN, K. S., SCHMEEDLIN -REN, P., GHOSH, M., FANG, C., WRIGHTON, S. A., MERION, R. M. and WATKINS, P. B., 1994, CYP3A gene expression in human gut epithelium. Pharmaco-genetics, 4, 247–259.
  • KRISHNA, D. R. and KLOTZ, U., 1994, Extrahepatic metabolism of drugs in humans. Clinical Pharmacokineticsks, 26, 144–160.
  • KUPFERSCHMIDT, H. H., FATTINGER, K. E., HA, H. R., FOLLATH, F. and KRAHENBUHL, S., 1998, Grapefruit juice enhances the bioavailability of the HIV protease inhibitor saquinavir in man. British Journal of Clinical Pharmacology, 45, 355–359.
  • LAMPEN, A., CHRISTIANS, U., GONSCHIOR, A. K., BADER, A., HACKBARTH, I., VON ENGELHARDT, W. and SEWING, K. F., 1996, Metabolism of the macrolide immunosuppressant, tacrolimus, by the pig gut mucosa in the Ussing chamber. British Journal of Pharmacology, 117, 1730–1734.
  • LEVY, R., CZERNOBILSKY, B. and GEIGER, B., 1988, Subtyping of epithelial cells of normal and metaplastic human uterine cervix, using polypeptide-specific cytokeratin antibodies. Dffirentiation, 39, 185–196.
  • LIPKIN, M., 1973, Proliferation and differentiation of gastrointestinal cells. Physiology Reviews, 53, 891–915.
  • MCKINNON, R. A., BURGESS, W. M., HALL, P. M., ROBERTS -THOMSON, S. J., GONZALEZ, F. J. and McMANus, M. E., 1995, Characterisation of CYP3A gene subfamily expression in human gastrointestinal tissues. Gut, 36, 259–267.
  • Nrms, R. W., PROUGH, R. A. and LUBET, R. A., 1984, Cytosol-mediated reduction of resorufin: a method for measuring quinone oxidoreductase. Archives in Biochemistry and Biophysics, 229, 459-465. OKEY, A. B., 1990, Enzyme induction in the cytochrome P450 system. Pharmacology and Therapeutics, 45, 241–298.
  • OLSEN, A. K., HANSEN, K. T. and FRIES, C., 1997, Pig hepatocytes as an in vitro model to study the regulation of human CYP3A4—prediction of drug—drug interactions with 17-alpha-ethynyl-estradiol. Chemico-Biological Interactions, 107, 93–108.
  • ONO, S., HATANAKA, T., MIYAZAWA, S., TSUTSUI, M., ÃOYAMA, T., GONZALEZ, F. J. and SATOH, 1996, Human liver microsomal diazepam metabolism using cDNA-expressed cytochrome P450: role of CYP2B6,2C19 and the 3A subfamily. Xenobbtka, 26, 1155–1166.
  • PARKINSON, A., 1996, An overview of current cytochrome P450 technology for assessing the safety and efficacy of new materials. Toxicology and Pathology, 24, 45–57.
  • PERREAULT, N. and BEAULIEU, J. F., 1998, Primary cultures of fully differentiated and pure human intestinal epithelial cells. Experimental Cell Research, 25, 34–42.
  • RODRIGUES, A. D., 1994, Use of in vitro human metabolism studies in drug development. Biochemical Pharmacology, 48, 2147–2156.
  • SKAANILD, M. T. and FRIIS, C., 1998, Characterisation of the P450 system in Göttingen minipigs. Scandinavian Journal of Laboratory Animal Science, Suppl. 1., 25, 171.
  • Swm, P. K., KROHN, R. I., HERMANSON, G. T., MALLIA, A. K., GARTNER, F. H., PROVENZANO, M. D., FUJIMOTO, E. K., GOEKE, N. M., OLSON, B. J. and KLENK, D. C., 1985, Measurement of protein using bicinchoninic acid. Annals in Biochemistry, 150, 76–85.
  • SORELLE R, 1998, Withdrawal of Posicor from market. Circulation, 1, 831–832.
  • STRATER, J., WEDDING, U., BARTH, T. F., KORETZ, K., ELSING, C. and MOLLER, P., 1996, Rapid onset of apoptosis in vitro follows disruption of beta 1-integrin/matrix interactions in human colonic crypt cells. Gastroenterology, 110, 1776–1784.
  • Suzum, K., YOSHIMURA, Y., HISADA, Y. and MATSUMOTO, A., 1994, Sensitivity of intestinal alkaline phosphatase to L-homoarginine and its regulation by subunit—subunit interaction. Japanese Journal of Pharmacology, 64, 97–102.
  • THUMIVIEL, K. E., PAIN, M. F., SHEN, D. D., KUNZE, K. L., PERKINS, J. D. and WILKINSON, G. R., 1996, Oral first-pass elimination of midazolam involves both gastrointestinal and hepatic CYP3A-mediated metabolism. Clinical Pharmacology and Therapeutics, 59, 491–502.
  • TUCKER, G. T., 1992, The rational selection of drug interaction studies: Implication of recent advances in drug metabolism. Internationd Journal of Clinical Pharmacology and Therapeutk Toxicology, 30, 550–553.
  • WHITLOCK, J. P., JR., OKINO, S. T., DONG, L., Ko, H. P., CLARKE -KATZENBERG, R., MA, Q. and LI, H., 1996, Cytochromes P450 5: Induction of cytochrome P450 1A1: a model for analyzing mammalian gene transcription. FASEB Journal, 10, 809–818.
  • WITRAmP, R. and MONSHOUWER, M., 1998, Pharmakokinetics in vivo and in vitro in swine. Scandinavian Journal of Laboratory Animal Science Suppl. 1, 25, 45–56.
  • WRIGHTON, S. A., RING, B. J. and VANDEN BRANDEN, M., 1995, The use of in vitro metabolism techniques in the planning and interpretation of drug safety studies. Toxicology and Pathology, 23, 199–208.
  • Wu, C. Y., BENET, L. Z., HEBERT, M. F., GUPTA, S. K., ROWLAND, M., GOMEZ, D. Y. and WACHER, V. J., 1995, Differentiation of absorption and first-pass gut and hepatic metabolism in humans: studies with cyclosporine. Clinical Pharmacology and Therapeutics, 58, 492–497.
  • ZAHER, H., YANG, T. J., GELBOIN, H. V., FERNANDEZ -SALGUERO, P. and GONZALEZ, F. J., 1998, Effect of phenobarbital on hepatic CYP1A1 and CYP1A2 in the Ahr-null mouse. Biochemical Phar-macology, 55, 235–238.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.