Crem Activator Isoforms in Normal and Impaired Human Spermatogenesis Analyzed by Real Time RT-PCR

REFERENCES

• Behr, R., Hunt, N., Ivell, R., Wessels, J. and Weinbauer, G. F. (2000) Cloning and expression analysis of testis-specific cyclic 3′,5′-adenosine monophosphate-responsive element modulator activators in the nonhuman primate (Macaca fascicularis): Comparison with other primate and rodent species. Biol Reprod 6:1344–1351.
   Web of Science ®Google Scholar
• Behr, R. and Weinbauer, G. F. (1999) Germ cell-specific cyclic adenosine 3′,5′-monophosphate response element modulator expression in rodent and primate testis is maintained despite gonadotropin deficiency. Endocrinology 140:2746–2754.
   PubMed Web of Science ®Google Scholar
• Behr, R. and Weinbauer, G. F. (2000) CREM activator and repressor isoforms in human testis: Sequence variations and inaccurate splicing during impaired spermatogenesis. Mol Hum Reprod 6:967–972.
   PubMed Web of Science ®Google Scholar
• Behr, R. and Weinbauer, G. F. (2001) cAMP response element modulator (CREM): An essential factor for spermatogenesis in primates? Int J Androl 24:126–135.
   PubMedGoogle Scholar
• Blendy, J. A., Kaestner, K. H., Weinbauer, G. F., Nieschlag, E. and Schutz, G. (1996) Severe impairment of spermatogenesis in mice lacking the CREM gene. Nature 380:162–165.
   PubMed Web of Science ®Google Scholar
• Blöcher, S., Behr, R., Weinbauer, G. F., Bergmann, M. and Steger, K. (2003) Different CREM-isoform gene expression between equine and human normal and impaired spermatogenesis. Theriogenology 60:1357–1369.
   PubMed Web of Science ®Google Scholar
• Blöcher, S., Fink, L., Bohle, R. M., Bergmann, M. and Steger, K. (2005) CREM activator and repressor isoform expression in human male germ cells. Int J Androl 28:215–223.
   PubMedGoogle Scholar
• Chomczynski, P. and Sacchi, N. (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162:156–159.
   PubMed Web of Science ®Google Scholar
• Daniel, P. B., Rohrbach, L. and Habener, J. F. (2000) Novel cyclic adenosine 3′,5′-monophosphate (cAMP) response element modulator theta isoforms expressed by two newly identified cAMP-responsive promoters active in the testis. Endocrinology 141:3923–3930.
   PubMed Web of Science ®Google Scholar
• De Cesare, D., Fimia, G. M. and Sassone-Corsi, P. (2000) CREM, a master-switch of the transcriptional cascade in male germ cells. J Endocrinol Invest 23:592–596.
   PubMed Web of Science ®Google Scholar
• Delmas, V., Laoide, B. M., Masquilier, D., de Groot, R. P., Foulkes, N. S. and Sassone-Corsi, P. (1992) Alternative usage of initiation codons in mRNA encoding the cAMP-responsive-element modulator generates regulators with opposite functions. Proc Natl Acad Sci USA 89:4226–4230.
   PubMed Web of Science ®Google Scholar
• Delmas, V., van der Hoorn, F., Mellstrom, B., Jegou, B. and Sassone-Corsi, P. (1993) Induction of CREM activator proteins in spermatids: Down-stream targets and implications for haploid germ cell differentiation. Mol Endocrinol 7:1502–1514.
   PubMed Web of Science ®Google Scholar
• Fimia, G. M., De Cesare, D. and Sassone-Corsi, P. (1999) CBP-independent activation of CREM and CREB by the LIM-only protein ACT. Nature 398:165–169.
   PubMed Web of Science ®Google Scholar
• Foulkes, N. S., Borrelli, E. and Sassone-Corsi, P. (1991) CREM gene: Use of alternative DNA-binding domains generates multiple antagonists of cAMP-induced transcription. Cell 64:739–749.
   PubMed Web of Science ®Google Scholar
• Foulkes, N. S., Mellstrom, B., Benusiglio, E. and Sassone-Corsi, P. (1992) Developmental switch of CREM function during spermatogenesis: From antagonist to activator. Nature 355:80–84.
   PubMed Web of Science ®Google Scholar
• Gellersen, B., Kempf, R., Sandhowe, R., Weinbauer, G. F. and Behr, R. (2002) Novel leader exons of the cyclic adenosine 3′,5′-monophosphate response element modulator (CREM) gene, transcribed from promoters P3 and P4, are highly testis-specific in primates. Mol Hum Reprod 8:965–976.
   PubMed Web of Science ®Google Scholar
• Hogeveen, K. N. and Sassone-Corsi, P. (2006) P regulation of gene expression in post-meiotic male germ cells: CREM-signalling pathways and male fertility. Hum Fertil (Camb) 9:73–79.
   PubMedGoogle Scholar
• Janitz, M., Fiszer, D., Michalczak-Janitz, K., Lukaszyk, A., Fernandez, N., Skorupski, W. and Kurpisz, M. (1994) Analysis of mRNA for class I HLA on human gametogenic cells. Mol Reprod Dev 38:231–237.
   PubMed Web of Science ®Google Scholar
• Jaspers, S., Gellersen, B., Kempf, R., Samalecos, A., Bergmann, M. and Steger, K. (2007) Functional characterization of male germ cell-specific CREM isoforms. J Androl 28:59–66.
   PubMedGoogle Scholar
• Lamas, M. and Sassone-Corsi, P. (1997) The dynamics of the transcriptional response to cyclic adenosine 3′,5′-monophosphate: Recurrent inducibility and refractory phase. Mol Endocrinol 11:1415–1424.
   PubMedGoogle Scholar
• Lin, W. W., Lamb, D. J., Lipshultz, L. I. and Kim, E. D. (1998) Absence of cyclic adenosine 3′:5′ monophosphate responsive element modulator expression at the spermatocyte arrest stage. Fertil Steril 69:533–538.
   PubMed Web of Science ®Google Scholar
• Nantel, F., Monaco, L., Foulkes, N. S., Masquilier, D., LeMeur, M., Henriksen, K., Dierich, A., Parvinen, M. and Sassone-Corsi, P. (1996) Spermiogenesis deficiency and germ-cell apoptosis in CREM-mutant mice. Nature 380:159–162.
   PubMed Web of Science ®Google Scholar
• Peri, A. and Serio, M. (2000) The CREM system in human spermatogenesis. J Endocrinol Invest 23:578–583.
   PubMed Web of Science ®Google Scholar
• Steger, K., Behr, R., Kleiner, I., Weinbauer, G. F. and Bergmann, M. (2004) Expression of activator of CREM in the testis (ACT) during normal and impaired spermatogenesis: Correlation with CREM expression. Mol Hum Reprod 10:129–135.
   PubMed Web of Science ®Google Scholar
• Steger, K., Klonisch, T., Gavenis, K., Behr, R., Schaller, V., Drabent, B., Doenecke, D., Nieschlag, E., Bergmann, M. and Weinbauer, G. F. (1999) Round spermatids show normal testis-specific H1t but reduced cAMP-responsive element modulator and transition protein 1 expression in men with round-spermatid maturation arrest. J Androl 20:747–754.
   PubMedGoogle Scholar
• Weinbauer, G. F., Behr, R., Bergmann, M. and Nieschlag, E. (1998) Testicular cAMP responsive element modulator (CREM) protein is expressed in round spermatids but is absent or reduced in men with round spermatid maturation arrest. Mol Hum Reprod 4:9–15.
   PubMed Web of Science ®Google Scholar
• Wistuba, J., Schlatt, S., Cantauw, C., von Schonfeldt, V., Nieschlag, E. and Behr, R. (2002) Transplantation of wild-type spermatogonia leads to complete spermatogenesis in testes of cyclic 3′,5′-adenosine monophosphate response element modulator-deficient mice. Biol Reprod 67:1052–1057.
   PubMed Web of Science ®Google Scholar
• Yin, J. L., Shackel, N. A., Zekry, A., McGuinness, P. H., Richards, C., Putten, K. V., McCaughan, G. W., Eris, J. M. and Bishop, G. A. (2001) Real-time reverse transcriptase-polymerase chain reaction (RT-PCR) for measurement of cytokine and growth factor mRNA expression with fluorogenic probes or SYBR Green I. Immunol Cell Biol 79:213–221.
   PubMed Web of Science ®Google Scholar