99
Views
0
CrossRef citations to date
0
Altmetric
Basic Research

Effect of platelet rich plasma versus melatonin on testicular injury induced by Busulfan in adult albino rats: a histological and immunohistochemical study

, , &
Pages 192-212 | Received 11 Dec 2023, Accepted 20 Feb 2024, Published online: 29 Feb 2024

References

  • Panahi S, Karamian A, Sajadi E, et al. Sertoli cell–conditioned medium restores spermatogenesis in azoospermic mouse testis. Cell Tissue Res. 2020 Mar;379(3):577–587. doi:10.1007/s00441-019-03092-w. PMID: 31494714.
  • Ozdemir ZN, Bozdag SC. Hematological malignancies and fertility. Cell biology and translational medicine. In: Turksen K, ed. Cell Biology and Translational Medicine, Volume 9. Advances in Experimental Medicine and Biology. Vol. 1288, Cham: Springer; 2020:pp. 103–115. doi: 10.1007/5584_2020_490.PMID: 32124411.
  • Zao JH, Schechter T, Liu WJ, et al. Performance of busulfan dosing guidelines for pediatric hematopoietic stem cell transplant conditioning. Biol Blood Marrow Transplant. 2015 Aug 1;21(8):1471–1478. doi:10.1016/j.bbmt.2015.05.006. Epub 2015 May 11. PMID: 25977229.
  • Liu FJ, Dong WY, Zhao H, Shi XH, Zhang YL. Effect of molybdenum on reproductive function of male mice treated with busulfan. Theriogenology. 2019 Mar 1;126:49–54. doi:10.1016/j.theriogenology.2018.12.002. Epub 2018 Dec 3. PMID: 30530157.
  • Krivoy N, Hoffer E, Lurie Y, Bentur Y, Rowe JM. Busulfan use in hematopoietic stem cell transplantation: pharmacology, dose adjustment, safety and efficacy in adults and children. CDS. 2008 Jan 1;3(1):60–66. doi:10.2174/157488608783333899. PMID: 18690982.
  • Krivoy N, Hoffer E, Lurie Y, Bentur Y, Rowe JM. Monitoring of busulphan concentrations in children undergone hematopoietic stem cell transplantation: unicentric experience over 10 years. Eur J Drug Metab Pharmacokinet. 2018 Apr;43(2):173–181. doi:10.1007/s13318-017-0431-0. PMID: 28801891.
  • Bashir Q, Thall PF, Milton DR, et al. Conditioning with busulfan plus melphalan versus melphalan alone before autologous haemopoietic cell transplantation for multiple myeloma: an open-label, randomised, phase 3 trial. Lancet Haematol. 2019 May 1;6(5):e266–e275. doi:10.1016/S2352-3026(19)30023-7. PMID: 30910541.
  • Hakemi SG, Sharififar F, Haghpanah T, Babaee A, Eftekhar-Vaghefi SH. The effects of olive leaf extract on the testis, sperm quality and testicular germ cell apoptosis in male rats exposed to busulfan. International Journal Of Fertility & Sterility. 2019 Apr;13(1):57. doi:10.22074/ijfs.2019.5520. PMID: 30644246.
  • Abofoul-Azab M, Lunenfeld E, Levitas E, et al. Identification of premeiotic, meiotic, and postmeiotic cells in testicular biopsies without sperm from Sertoli cell-only syndrome patients. IJMS. 2019 Jan 22;20(3):470. doi:10.3390/ijms20030470. PMID: 30678285.
  • Tordjman S, Chokron S, Delorme R, et al. Melatonin: pharmacology, functions and therapeutic benefits. CN. 2017 Apr 1;15(3):434–443. doi:10.2174/1570159X14666161228122115. PMID: 28503116.
  • Acuña-Castroviejo D, Escames G, Venegas C, Díaz-Casado ME, Lima-Cabello E, López LC, Rosales-Corral S, Tan DX, Reiter RJ. Extrapineal melatonin: sources, regulation, and potential functions. Cell Mol Life Sci. Aug, 2014;71(16):2997–3025. doi:10.1007/s00018-014-1579-2. PMID: 24554058.
  • Reiter RJ, Tan DX, Rosales-Corral S, Galano A, Zhou XJ, Xu B. Mitochondria: central organelles for melatonin′ s antioxidant and anti-aging actions. Molecules. 2018 Feb 24;23(2):509. doi:10.3390/molecules23020509. PMID: 29495303.
  • Reiter RJ, Mayo JC, Tan DX, Sainz RM, Alatorre‐Jimenez M, Qin L. Melatonin as an antioxidant: under promises but over delivers. J Pineal Res. 2016 Oct;61(3):253–278. doi:10.1111/jpi.12360. PMID: 27500468.
  • Alves R, Grimalt R. A review of platelet-rich plasma: history, biology, mechanism of action, and classification. Skin appendage disorders. Skin Appendage Disord. 2018 Jan 16;4(1):18–24. doi:10.1159/000477353. PMID: 29457008.
  • Samy A, Abbas AM, Elmoursi A, Elsayed M, Hussein RS. Effect of autologous platelet-rich plasma transfusion in the treatment of infertile women with thin endometrium and its implications in IVF cycles: a literature review. Middle East Fertil Soc J. 2020 Dec;25(1):1–6. doi:10.1186/s43043-020-0019-5.
  • Mussano F, Genova T, Munaron L, Petrillo S, Erovigni F, Carossa S. Cytokine, chemokine, and growth factor profile of platelet-rich plasma. Platelets. 2016 Jul 3;27(5):467–471. doi:10.3109/09537104.2016.1143922. PMID: 26950533.
  • Dönmez Mİ, İnci K, Zeybek ND, Doğan HS, Ergen A. The early histological effects of intravesical instillation of platelet-rich plasma in cystitis models. Int Neurourol J. 2016 Sep;20(3):188–196. doi:10.5213/inj.1632548.274. PMID: 27706013.
  • Dönmez Mİ, İnci K, Zeybek ND, Doğan HS, Ergen A. Anticoagulants interfere with the angiogenic and regenerative responses mediated by platelets. Frontiers in bioengineering and biotechnology. Front Bioeng Biotechnol. 2020 Mar 20;8:223. doi:10.3389/fbioe.2020.00223. PMID: 32266247.
  • Pazzini JM, Nardi AB, Huppes RR, et al. Method to obtain platelet-rich plasma from rabbits (Oryctolagus cuniculus). Pesquisa Veterinária Brasileira. 2016;36(1):39–44. doi:10.1590/S0100-736X2016000100007.
  • Arshdeep M, Platelet-rich plasma in dermatology: boon or a bane? Indian J Dermatol Venereol Leprol. 2014 Jan 1;80(1):5–14. doi:10.4103/0378-6323.125467. PMID: 24448117.
  • Zhang X, Xia Q, Wei R, et al. Melatonin protects spermatogonia from the stress of chemotherapy and oxidation via eliminating reactive oxidative species. Free Radical Biol Med. 2019 Jun 1;137:74–86. doi:10.1016/j.freeradbiomed.2019.04.009 PMID: 30986493.
  • Dehghani F, Sotoude N, Bordbar H, Panjeshahin MR, Karbalay-Doust S. The use of platelet-rich plasma (PRP) to improve structural impairment of rat testis induced by busulfan. Platelets. 2019 May 19;30(4):513–520. doi:10.1080/09537104.2018.1478400. PMID: 29883240.
  • Somade OT, Ajiboye BO, Osukoya OA, Jarikre TA, Oyinloye BE. Syringic acid ameliorates testicular oxidative stress via the conservation of endogenous antioxidant markers and inhibition of the activated Nrf2-Keap1-NQO1-HO1 signaling in methyl cellosolve-administered rats. Pharmacol Res Mod Chin 2023 Mar 1;6:100207. doi:10.1016/j.prmcm.2022.100207.
  • Teilmann AC, Nygaard Madsen A, Holst B, Hau J, Rozell B, Abelson KS. Physiological and pathological impact of blood sampling by retro-bulbar sinus puncture and facial vein phlebotomy in laboratory mice. PLoS One. 2014 Nov 26;9(11):e113225. doi:10.1371/journal.pone.0113225. PMID: 25426941.
  • Kei S. Serum lipid peroxide in cerebrovascular disorders determined by a new colorimetric method. Clin Chim Acta. 1978 Nov 15;90(1):37–43. doi:10.1016/0009-8981(78)90081-5. PMID: 719890.
  • Wen Q, Wang Y, Tang J, Cheng CY, Liu YX, Ramchandran R. Sertoli cell Wt1 regulates peritubular myoid cell and fetal Leydig cell differentiation during fetal testis development. PLoS One. 2016 Dec 30;11(12):e0167920. doi:10.1371/journal.pone.0167920. PMID: 28036337.
  • Suvarna KS, Layton C, Bancroft JD. Bancroft’s theory and practice of histological techniques. 8th ed. Elsevier; 2019:40–138.
  • Magaki S, Hojat SA, Wei B, So A, Yong WH. An introduction to the performance of immunohistochemistry. Bio Meth and Protoc 2019:289–298. doi:10.1007/978-1-4939-8935-5_25. PMID: 30539453.
  • Tizro P, Choi C, Khanlou N. Sample Preparation for Transmission Electron Microscopy. Bio Meth and Protoc 2019pp. 417–424. doi:10.1007/978-1-4939-8935-5_33 PMID: 30539461.
  • Johnsen SG. Testicular biopsy score count – a method for registration of spermatogenesis in human testes: normal values and results in 335 hypogonadal males. Horm Res Paediatr. 1970;1(1):2–25. doi:10.1159/000178170. PMID: 5527187.
  • Zhang K, Fu L, An Q, et al. Effects of Qilin pills on spermatogenesis, reproductive hormones, oxidative stress, and the TSSK2 gene in a rat model of oligoasthenospermia. BMC complement med. 2020 Dec;20(1):1–11. doi:10.1186/s12906-019-2799-7. PMID: 32046715.
  • Rahayu I, Usman E, Reza M. Effect of vitamin C on testosterone level, sperm count and sperm morphology in gentamicin-induced Wistar rats. Int J Res Med Sci. 2019;7(2):451–456. doi:10.18203/2320-6012.ijrms20190352.
  • Faruk EM, Ibrahim MH, Eid EM, Shalaby SA, Ebrahim OF. Comparative study between bone marrow-mesenchymal stem cells and adipose tissue-mesenchymal stem cells in restoration of male fertility. JPRI. 2022 Mar;21:44–69. doi:10.9734/jpri/2022/v34i26A35971.
  • Ahmed AI, Lasheen NN, El-Zawahry KM. Ginkgo biloba ameliorates subfertility induced by testicular ischemia/reperfusion injury in adult Wistar rats: a possible new mitochondrial mechanism. Oxid Med Cell Longev. Jan 1, 2016;2016:6959274. doi:10.1155/2016/6959274. PMID: 28101298.
  • Chen H, Mruk D, Xiao X, Cheng CY. Human spermatogenesis and its regulation. Male hypogonadism: basic, clinical and therapeutic principles. In: Winters S Huhtaniemi I, eds. Male Hypogonadism. Contemporary Endocrinology. Cham: Humana Press; 2017:pp. 49–72. doi:10.1007/978-3-319-53298-13.
  • Gholampour F, Malekpour Mansourkhani S, Owji SM. Amelioration of testicular damages in renal ischemia/reperfusion by berberine: an experimental study. IJRM. 2019 Nov 10;17(11):799–806. doi:10.18502/ijrm.v17i10.5488. PMID: 31911962.
  • Anan HH, Zidan RA, Abd EL-Baset SA, Ali MM. Ameliorative effect of zinc oxide nanoparticles on cyclophosphamide induced testicular injury in adult rat. Tissue And Cell. 2018 Oct 1;54:80–93. doi:10.1016/j.tice.2018.08.006. PMID: 30309514.
  • Levine S, Saltzman A, Ginsberg SD. Vacuolar pathology in the median eminence of the hypothalamus after hyponatremia. J Neuropath Exp Neurol. 2011 Feb 1;70(2):151–156. doi:10.1097/NEN.0b013e318208fc5d. PMID: 21343884.
  • Fotouh GI, Abdel-Dayem MM, Ismail DI, Mohamed HH. Histological study on the protective effect of endogenous stem cell mobilization in busulfan-induced testicular injury in albino rats. J Microsc Ultrastruct. Oct, 2018;6(4):197–204. PMID: 30464893. doi:10.4103/JMAU.JMAU_35_18.
  • Mahmoud AM, Nor-Eldin EK, Elsayed HM. The possible protective effect of Omega 3 fatty acids against bisphenol a induced disruption of pituitary-testicular axis in albino rat. Biohemical, histological and immunohistochemical study. Biochem Histol Immun Hist Chem study Egpytian J Histol. 2019 Mar 1;42(1):215–228. doi:10.21608/ejh.2018.5474.1030.
  • El-Beltagi EM, Elwan WM, El-Bakry NA, Salah EF. Histological and immunohistochemical study on the effect of gibberellic acid on the seminiferous tubules of testis of adult albino rat and the possible protective role of grape seeds proanthocyanidin extract. Tanta Med J. 2017 Apr 1;45(2):79. doi:10.4103/tmj.tmj_38_16.
  • Qu N, Itoh M, Sakabe K. Effects of chemotherapy and radiotherapy on spermatogenesis: the role of testicular immunology. IJMS. 2019 Feb 22;20(4):957. doi:10.3390/ijms20040957. PMID: 30813253.
  • Mitchell RN. The cell as a unit of health and disease. In: Kumar V, Abbas A Aster J, eds. Robbins and Cotran Pathologic Basis of Disease. 10th ed. New York: Elsevier Health Sciences; 2018:pp. 1–30.
  • Fang F, Ni K, Cai Y, et al. Busulfan administration produces toxic effects on epididymal morphology and inhibits the expression of ZO-1 and vimentin in the mouse epididymis. Biosci Rep. 37(6). 2017 Dec 12. doi:10.1042/BSR20171059. PMID: 29101242.
  • Abd El Salam L. Light and electron microscopic study on the possible protective effect of melatonin on formaldehyde induced testicular damage in adult albino rats. Egypt J Histol. 2020 Dec 1;43(4):1047–1058. doi:10.21608/ejh.2020.19282.1199.
  • Meligy FY, Abo Elgheed AT, Alghareeb SM. Therapeutic effect of adipose-derived mesenchymal stem cells on Cisplatin induced testicular damage in adult male albino rat. Ultrastruct Pathol. 2019 Jan 2;43(1):28–55. doi:10.1080/01913123.2019.1572256. PMID: 30741078.
  • Abou Elghait AT, Mostafa TM, Gameaa FK, Mohammed GK, Meligy FY, Sayed MM. Comparative histological study on the effect of tramadol abuse on the testis of juvenile and adult male albino mice. Anat Cell Biol. 2022 Sep 1;55(3):341–355. doi:10.5115/acb.22.013. PMID: 36008129.
  • Sasso-Cerri E, Oliveira B, de Santi F, Beltrame FL, Caneguim BH, Cerri PS. The antineoplastic busulphan impairs peritubular and leydig cells, and vitamin B12 stimulates spermatogonia proliferation and prevents busulphan-induced germ cell death. Biomed Pharmacother. 2017 Nov 1;95:1619–1630. doi:10.1016/j.biopha.2017.08.131. PMID: 28950662.
  • Ibrahim HF, Safwat SH, Zeitoun TM, El Mulla KF, Medwar AY. The therapeutic potential of amniotic fluid-derived stem cells on busulfan-induced azoospermia in adult rats. Tissue Eng Regen Med. 2021 Apr;18(2):279–295. doi:10.1007/s13770-020-00309-w. PMID: 33713308.
  • Ahmed RR, Abdul-Hamid M, Galaly SR, Hamdalla HM. Monosodium glutamate-induced liver microscopic and biochemical changes in male rats, and the possible amendment of quercetin. Egpytian J Zool. 2019 Jun 1;71(71):44–55. doi:10.21608/ejz.2019.37158.
  • Cai Y, Liu T, Fang F, Shen S, Xiong C. Involvement of ICAM‐1 in impaired spermatogenesis after busulfan treatment in mice. Andrologia. 2016 Feb;48(1):37–44. doi:10.1111/and.12414. PMID: 25808259.
  • Jiang S, Xu Y, Fan Y, Hu Y, Zhang Q, Su W. Busulfan impairs blood–testis barrier and spermatogenesis by increasing noncollagenous 1 domain peptide via matrix metalloproteinase 9. Andrology. 2022 Feb;10(2):377–391. doi:10.1111/andr.13112. PMID: 34535976.
  • Chen P, Chen F, Zhou B. Antioxidative, anti-inflammatory and anti-apoptotic effects of ellagic acid in liver and brain of rats treated by D-galactose. Sci Rep. 2018 Jan 23;8(1):1465. doi:10.1038/s41598-018-19732-0. PMID: 29362375.
  • Choi YJ, Ok DW, Kwon DN, et al. Murine male germ cell apoptosis induced by busulfan treatment correlates with loss of c-kit-expression in a Fas/FasL-and p53-independent manner. FEBS Lett. 2004 Sep 24;575(1–3):41–51. doi:10.1016/j.febslet.2004.08.034. PMID: 15388331.
  • Gouda ZA, Selim AO. A possible correlation between the testicular structure and short photoperiod exposure in young albino rats: light and electron microscopic study. Egypt J Histol. 2013 Mar 1;36(1):28–38. doi:10.1097/01.EHX.0000423980.95382.0c.
  • Mansour AM, Ibrahim MA, Laag EM, Zamzam AF. Histological Study of the Effect of Semicarbazide on Testicular Seminiferous Tubules of Juvenile Albino Rat. Ain Shams Med J. 2018 Jan 1;30(1):27–37. doi:10.21608/ajfm.2018.18134.
  • Hazrati A, Salimnejad R, Alipour MR, Mirzaei Bavil F, Alihemmati A. Protective effect of ghrelin on testicular damages caused by chronic hypoxia in rats: a histopathological study. Andrologia. 2018;50(4):e12989. doi:10.1111/and.12989. Epub ahead of print. PMID: 29460339.
  • Hasanin NA, Sayed NM, Ghoneim FM, Al-Sherief SA. Histological and ultrastructure study of the testes of acrylamide exposed adult male albino rat and evaluation of the possible protective effect of vitamin E intake. J Microsc Ultrastruct. 2018;6(1):23–34. PMID: 30023264. doi:10.4103/JMAU.JMAU_7_18.
  • Ahmed MA. Amelioration of nandrolone decanoate-induced testicular and sperm toxicity in rats by taurine: effects on steroidogenesis, redox and inflammatory cascades, and intrinsic apoptotic pathway. Toxicol Appl Pharmacol. 2015 Feb 1;282(3):285–296. doi:10.1016/j.taap.2014.12.007. PMID: 25542992.
  • Thakur M, Gupta H, Singh D, et al. Histopathological and ultra structural effects of nanoparticles on rat testis following 90 days (chronic study) of repeated oral administration. J Nanobiotechnol. 2014;12(1):1–13. doi:10.1186/s12951-014-0042-8. PMID: 25311086.
  • Sun TC, Li HY, Li XY, Yu K, Deng SL, Tian L. Protective effects of melatonin on male fertility preservation and reproductive system. Cryobiology. 2020 Aug 1;95:1–8. doi:10.1016/j.cryobiol.2020.01.018. PMID: 32001217.
  • Zhang HM, Zhang Y. Melatonin: a well‐documented antioxidant with conditional pro‐oxidant actions. J Pineal Res. 2014 Sep;57(2):131–146. doi:10.1111/jpi.12162. PMID: 25060102.
  • Salehi B, Sharopov F, Fokou PV, et al. Melatonin in medicinal and food plants: occurrence, bioavailability, and health potential for humans. Cells. 2019 Jul 5;8(7):681. doi:10.3390/cells8070681. PMID: 31284489.
  • Doerrier C, García JA, Volt H, et al. Permeabilized myocardial fibers as model to detect mitochondrial dysfunction during sepsis and melatonin effects without disruption of mitochondrial network. Mitochondrion. 2016 Mar 1;27:56–63. doi:10.1016/j.mito.2015.12.010. PMID: 26748191.
  • Xu P, Wu Y, Zhou L, et al. Platelet-rich plasma accelerates skin wound healing by promoting re-epithelialization. Burns Trauma. 2020;8:tkaa028. doi:10.1093/burnst/tkaa028. PMID: 32821743.
  • Egle K, Salma I, Dubnika A. From blood to regenerative tissue: how autologous platelet-rich fibrin can be combined with other materials to ensure controlled drug and growth factor release. IJMS. 2021 Oct 26;22(21):11553. doi:10.3390/ijms222111553. PMID: 34768984.
  • Opneja A, Kapoor S, Stavrou EX. Contribution of platelets, the coagulation and fibrinolytic systems to cutaneous wound healing. Thromb Res. 2019 Jul 1;179:56–63. doi:10.1016/j.thromres.2019.05.001. PMID: 31078121.
  • Salem N, Helmi NM, Assaf N. Renoprotective effect of platelet-rich plasma on cisplatin-induced nephrotoxicity in rats. Oxid Med Cell Longev. 2018;2018:9658230. doi:10.1155/2018/9658230. PMID: 30116500.
  • Martini LI, Via AG, Fossati C, Randelli F, Randelli P, Cucchi D. Single platelet-rich plasma injection for early stage of osteoarthritis of the knee. Joints. 2017 Mar;5(1):2–6. PMID: 29114622. doi:10.1055/s-0037-1601405.
  • Marini MG, Perrini C, Esposti P, et al. Effects of platelet-rich plasma in a model of bovine endometrial inflammation in vitro. Reprod Biol Endocrinol. Dec, 2016; 14(1):1–17. doi:10.1186/s12958-016-0195-4. PMID: 27619959.
  • Ghai B, Gupta V, Jain A, Goel N, Chouhan D, Batra YK. Effectiveness of platelet rich plasma in pain management of osteoarthritis knee: double blind, randomized comparative study. Rev Bras Anestesiol. 2019 Dec 20;69(5):439–447. doi:10.1016/j.bjan.2019.06.003. PMID: 31672420.
  • Ezim OO, Abarikwu SO, Banihani S. Exogenous Ascorbate Administration Elevates Testicular Oxidative Damage and Histological Injuries in Rats after Busulfan Treatment. Andrologia. 2023 Jun 30;2023:1–10. doi:10.1155/2023/5209480.
  • Food and Drug Administration. Guidance for industry: estimating the maximum safe starting dose in initial clinical trials for therapeutics in adult healthy volunteers. https://www.fda.gov/regulatory-information/search-fda-guidance-documents/estimating-maximum-safe-starting-dose-initial-clinical-trials-therapeutics-adult-healthy-volunteers. 2005. Last accessed December 2, 2023.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.