Advanced search
Publication Cover
Immunological Investigations
A Journal of Molecular and Cellular Immunology
Volume 36, 2007 - Issue 4
Submit an article Journal homepage
116
Views
3
CrossRef citations to date
0
Altmetric
Original

Rapid HLA-DR Fluorotyping Based on Melting Curve Analysis

Yang Liu Laboratory of Molecular Immunology, State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai, China
,
Qiyun Zhu Laboratory of Molecular Immunology, State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai, China
&
Naishuo Zhu Laboratory of Molecular Immunology, State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai, ChinaCorrespondence[email protected]
Pages 507-521 | Published online: 07 Jul 2009

REFERENCES

  • Albis-Camps M., Blasczyk R. Fluorotyping of HLA-DRB by sequence-specific priming and fluorogenic probing. Tissue Antigens 1999; 53: 301–307
  • Beuret C. Simultaneous detection of enteric viruses by multiplex real-time RT-PCR. J. Virol. Meth. 2004; 115: 1–8
  • Bon M. A., van Oeveren-Dybicz A., van den Bergh F. A. Genotyping of HLA-B27 by real-time PCR without hybridization probes. Clin. Chem. 2000; 46: 1000–1002
  • Casamitjana N., Faner R., Santamaria A., Colobran R., Ribera A., Pujol-Borrell R., et al. Development of a new HLA-DRB real-time PCR typing method. Hum. Immunol. 2005; 66: 85–91
  • Dall'Ozzo S., Andres C., Bardos P., Watier H., Thibault G. Rapid single-step FCGR3A genotyping based on SYBR Green I fluorescence in real-time multiplex allele-specific PCR. J. Immunol. Meth. 2003; 277: 185–192
  • Dickinson A. M., Middleton P. G. Beyond the HLA typing age: Genetic polymorphisms predicting transplant outcome. Blood Rev. 2005; 19: 333–340
  • Faner R., Casamitjana N., Coll J., Caro P., Pujol-Borrell R., Palou E., et al. Real-time PCR using fluorescent resonance emission transfer probes for HLA-B typing. Hum. Immunol. 2006; 67: 374–385
  • Faner R., Casamitjana N., Colobran R., Ribera A., Pujol-Borrell R., Palou E., et al. HLA-B27 genotyping by fluorescent resonance emission transfer (FRET) probes in real-time PCR. Hum. Immunol. 2004; 65: 826–838
  • Li X. Y., Liu J. Z., Gao J. Z. Determination of a real-time fluorotyping strategy for the HLA-DR locus. Transpl. Proc. 2001; 33: 498–499
  • Olerup O., Zetterquist H. HLA-DR typing by PCR amplification with sequence-specific primers (PCR-SSP) in 2 hours: an alternative to serological DR typing in clinical practice including donor-recipient matching in cadaveric transplantation. Tissue Antigens 1992; 39: 225–235
  • Olerup O., Zetterguist H. DR “low-resolution” PCR-SSP typing-a correction and an up-date. Tissue Antigens 1993; 41: 55–56
  • Ririe K. M., Rasmussen R. P., Wittwer C. T. Product differentiation by analysis of DNA melting curves during the polymerase chain reaction. Anal. Biochem. 1997; 245: 154–160
  • Saiki R. K., Bugawan T. L., Horn G. T., Mullis K. B., Erlich H. A. Analysis of enzymatically amplified beta-globin and HLA-DQ alpha DNA with allele-specific oligonucleotide probes. Nature 1986; 324: 163–166
  • Slateva K., Camps M. A., Blasczyk R. Fluorotyping of HLA-A by sequence-specific priming and fluorogenic probing. Tissue Antigens 1998; 52: 462–472
  • Slateva K., Elsner H. A., Albis-Camps M., Blasczyk R. HLA-DRB fluorotyping by dark quenching and automated analysis. Tissue Antigens 2001; 58: 250–254
  • Sylvain K., Aurelie H., Marc M., Christophe R. Rapid screening for HLA-B27 by a TaqMan-PCR assay using sequence-specific primers and a minor groove binder probe, a novel type of TaqMan trade mark probe. J. Immunol. Meth. 2004; 287: 179–186
  • Tremmel M., Opelz G., Mytilineos J. High-resolution typing for HLA‐DRB1*15 amd -DRB1*16 by fluorescence-marked sequence-specific priming (TaqMan assay). Tissue Antigens 1999; 54: 508–516
  • Zhou L., Vandersteen J., Wang L., Fuller T., Taylor M., Patais B., et al. High-resolution DNA melting curve analysis to establish HLA genotypic identity. Tissue Antigen 2004; 64: 156–164

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Order Reprints Request Corporate Permissions

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

Request Academic Permissions

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.

Related research

People also read lists articles that other readers of this article have read.

Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.

Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.