Publication Cover
Immunological Investigations
A Journal of Molecular and Cellular Immunology
Volume 36, 2007 - Issue 5-6
182
Views
3
CrossRef citations to date
0
Altmetric
Original

Simultaneous Analysis of In Vivo CD8+ T Cell Cytotoxicity Against Multiple Epitopes using Multicolor Flow Cytometry

&
Pages 829-845 | Published online: 07 Jul 2009

REFERENCES

  • Ahonen C. L., Doxsee C. L., McGurran S. M., Riter T. R., Wade W. F., Barth R. J., Vasilakos J. P., Noelle R. J., Kedl R. M. Combined TLR and CD40 triggering induces potent CD8+ T cell expansion with variable dependence on type I IFN. J. Exp. Med. 2004; 199: 775–784
  • Badovinac V. P., Harty J. T. Intracellular staining for TNF and IFN-gamma detects different frequencies of antigen-specific CD8(+) T cells. J. Immunol. Meth. 2000; 238: 107–117
  • Barber D. L., Wherry E. J., Ahmed R. Cutting edge: rapid in vivo killing by memory CD8 T cells. J. Immunol. 2003; 171: 27–31
  • Betts M. R., Brenchley J. M., Price D. A., De Rosa S. C., Douek D. C., Roederer M., Koup R. A. Sensitive and viable identification of antigen-specific CD8+ T cells by a flow cytometric assay for degranulation. J. Immunol. Meth. 2003; 281: 65–78
  • Botten J., Alexander J., Pasquetto V., Sidney J., Barrowman P., Ting J., Peters B., Southwood S., Stewart B., Rodriguez-Carreno M. P., et al. Identification of protective Lassa virus epitopes that are restricted by HLA-A2. J. Virol. 2006; 80: 8351–8361
  • Botten J., Whitton J. L., Barrowman P., Sidney J., Whitmire J. K., Alexander J., Ting J. P., Bui H. H., Sette A., Buchmeier M. J. HLA-A2-restricted protection against lethal lymphocytic choriomeningitis. J. Virol. 2007; 81: 2307–2317
  • Bui H. H., Botten J., Fusseder N., Pasquetto V., Mothe B., Buchmeier M. J., Sette A. Protein sequence database for pathogenic arenaviruses. Immunome Res 2007; 3: 1
  • Burkett M. W., Shafer-Weaver K. A., Strobl S., Baseler M., Malyguine A. A novel flow cytometric assay for evaluating cell-mediated cytotoxicity. J. Immunother. 2005; 28: 396–402
  • Chen G., Tai A. K., Lin M., Chang F., Terhorst C., Huber B. T. Signaling lymphocyte activation molecule-associated protein is a negative regulator of the CD8 T cell response in mice. J. Immunol. 2005; 175: 2212–2218
  • Chiu C., Heaps A. G., Cerundolo V., McMichael A. J., Bangham C. R., Callan M. F. Early acquisition of cytolytic function and transcriptional changes in a primary CD8+ T-cell response in vivo. Blood 2007; 109: 1086–1094
  • De Rosa S. C., Lu F. X., Yu J., Perfetto S. P., Falloon J., Moser S., Evans T. G., Koup R., Miller C. J., Roederer M. Vaccination in humans generates broad T cell cytokine responses. J. Immunol. 2004; 173: 5372–5380
  • Ewen C. L., Rong J., Kokaji A. I., Bleackley R. C., Kane K. P. Evaluating antigen-specific cytotoxic T lymphocyte responses by a novel mouse granzyme B ELISPOT assay. J. Immunol. Meth. 2006; 308: 156–166
  • Fuse S., Bellfy S., Yagita H., Usherwood E. J. CD8+ T cell dysfunction and increase in murine gammaherpesvirus latent viral burden in the absence of 4-1BB ligand. J. Immunol. 2007; 178: 5227–5236
  • He L., Hakimi J., Salha D., Miron I., Dunn P., Radvanyi L. A sensitive flow cytometry-based cytotoxic T-lymphocyte assay through detection of cleaved caspase 3 in target cells. J. Immunol. Meth. 2005; 304: 43–59
  • Hermans I. F., Silk J. D., Yang J., Palmowski M. J., Gileadi U., McCarthy C., Salio M., Ronchese F., Cerundolo V. The VITAL assay: a versatile fluorometric technique for assessing CTL- and NKT-mediated cytotoxicity against multiple targets in vitro and in vivo. J. Immunol. Meth. 2004; 285: 25–40
  • Hinrichs C. S., Gattinoni L., Restifo N. P. Programming CD8+ T cells for effective immunotherapy. Curr. Opin. Immunol. 2006; 18: 363–370
  • Kaech S. M., Wherry E. J., Ahmed R. Effector and memory T-cell differentiation: implications for vaccine development. Nat. Rev. Immunol. 2002; 2: 251–262
  • Kotturi M. F., Peters B., Buendia-Laysa F., Jr., Sidney J., Oseroff C., Botten J., Grey H., Buchmeier M. J., Sette A. The CD8+ T-Cell response to lymphocytic choriomeningitis virus involves the L antigen: Uncovering new tricks for an old virus. J. Virol. 2007; 81: 4928–4940
  • Martin D. L., Weatherly D. B., Laucella S. A., Cabinian M. A., Crim M. T., Sullivan S., Heiges M., Craven S. H., Rosenberg C. S., Collins M. H., et al. CD8+ T-Cell responses to Trypanosoma cruzi are highly focused on strain-variant trans-sialidase epitopes. PLoS Pathog. 2006; 2: e77
  • Masopust D., Murali-Krishna K., Ahmed R. Quantitating the magnitude of the lymphocytic choriomeningitis virus-specific CD8 T-cell response: it is even bigger than we thought. J. Virol. 2007; 81: 2002–2011
  • Mescher M. F., Curtsinger J. M., Agarwal P., Casey K. A., Gerner M., Hammerbeck C. D., Popescu F., Xiao Z. Signals required for programming effector and memory development by CD8+ T cells. Immunol. Rev. 2006; 211: 81–92
  • Moutaftsi M., Peters B., Pasquetto V., Tscharke D. C., Sidney J., Bui H. H., Grey H., Sette A. A consensus epitope prediction approach identifies the breadth of murine T(CD8+)-cell responses to vaccinia virus. Nat. Biotechnol. 2006; 24: 817–819
  • Munks M. W., Gold M. C., Zajac A. L., Doom C. M., Morello C. S., Spector D. H., Hill A. B. Genome-wide analysis reveals a highly diverse CD8 T cell response to murine cytomegalovirus. J. Immunol. 2006; 176: 3760–3766
  • Murali-Krishna K., Altman J. D., Suresh M., Sourdive D. J., Zajac A. J., Miller J. D., Slansky J., Ahmed R. Counting antigen-specific CD8 T cells: a reevaluation of bystander activation during viral infection. Immunity 1998; 8: 177–187
  • Oehen S., Brduscha-Riem K. Differentiation of naive CTL to effector and memory CTL: correlation of effector function with phenotype and cell division. J. Immunol. 1998; 161: 5338–5346
  • Oehen S., Brduscha-Riem K., Oxenius A., Odermatt B. A simple method for evaluating the rejection of grafted spleen cells by flow cytometry and tracing adoptively transferred cells by light microscopy. J. Immunol. Meth. 1997; 207: 33–42
  • Oseroff C., Kos F., Bui H. H., Peters B., Pasquetto V., Glenn J., Palmore T., Sidney J., Tscharke D. C., Bennink J. R., et al. HLA class I-restricted responses to vaccinia recognize a broad array of proteins mainly involved in virulence and viral gene regulation. Proc. Natl. Acad. Sci. U S A 2005; 102: 13980–13985
  • Prasad S. J., Farrand K. J., Matthews S. A., Chang J. H., McHugh R. S., Ronchese F. Dendritic cells loaded with stressed tumor cells elicit long-lasting protective tumor immunity in mice depleted of CD4+CD25+ regulatory T cells. J. Immunol. 2005; 174: 90–98
  • Regoes R. R., Barber D. L., Ahmed R., Antia R. Estimation of the rate of killing by cytotoxic T lymphocytes. in vivo. Proc. Natl. Acad. Sci. U S A 2007; 104: 1599–1603
  • Slifka M. K., Whitton J. L. Functional avidity maturation of CD8(+) T cells without selection of higher affinity TCR. Nat. Immunol. 2001; 2: 711–717
  • Stambas J., Doherty P. C., Turner S. J. An in vivo cytotoxicity threshold for influenza A virus-specific effector and memory CD8(+) T cells. J. Immunol. 2007; 178: 1285–1292
  • Stevenson P. G., Belz G. T., Altman J. D., Doherty P. C. Changing patterns of dominance in the CD8+ T cell response during acute and persistent murine gammaherpesvirus infection. Eur. J. Immunol. 1999a; 29: 1059–1067
  • Stevenson P. G., Belz G. T., Castrucci M. R., Altman J. D., Doherty P. C. A gamma-herpesvirus sneaks through a CD8(+) T cell response primed to a lytic-phase epitope. Proc. Natl. Acad. Sci. U S A 1999b; 96: 9281–9286
  • Sunil-Chandra N. P., Efstathiou S., Arno J., Nash A. A. Virological and pathological features of mice infected with murine gamma-herpesvirus 68. J. Gen. Virol. 1992; 73: 2347–2356
  • Tscharke D. C., Karupiah G., Zhou J., Palmore T., Irvine K. R., Haeryfar S. M., Williams S., Sidney J., Sette A., Bennink J. R., Yewdell J. W. Identification of poxvirus CD8+ T cell determinants to enable rational design and characterization of smallpox vaccines. J. Exp. Med. 2005; 201: 95–104
  • Tscharke D. C., Woo W. P., Sakala I. G., Sidney J., Sette A., Moss D. J., Bennink J. R., Karupiah G., Yewdell J. W. Poxvirus CD8+ T-cell determinants and cross-reactivity in BALB/c mice. J. Virol. 2006; 80: 6318–6323
  • Usherwood E. J. A new approach to epitope confirmation by sampling effector/memory T cells migrating to the lung. J. Immunol. Meth. 2002; 266: 135–142
  • Zuber B., Levitsky V., Jonsson G., Paulie S., Samarina A., Grundstrom S., Metkar S., Norell H., Callender G. G., Froelich C., Ahlborg N. Detection of human perforin by ELISpot and ELISA: ex vivo identification of virus-specific cells. J. Immunol. Meth. 2005; 302: 13–25

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.