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Review Article

Ethosomes as a carrier for transdermal drug delivery system: methodology and recent developments

, , &
Received 01 Feb 2024, Accepted 02 Apr 2024, Published online: 27 Apr 2024

References

  • Goyal R, Macri LK, Kaplan HM, et al. Nanoparticles and nanofibers for topical drug delivery. J Control Release. 2016;240:77–92. doi: 10.1016/j.jconrel.2015.10.049.
  • Liu L, Zhao W, Ma Q, et al. Functional nano-systems for transdermal drug delivery and skin therapy. Nanoscale Adv. 2023;5(6):1527–1558. doi: 10.1039/d2na00530a.
  • Basu B, Dutta A, Parihar A, et al. Ethosomes: A Lipid-based drug delivery system. Lipid-Based Drug Deliv Syst Princ Appl Jan 2023; 223–297. doi: 10.1201/9781003459811-8.
  • Jeong WY, Kwon M, Choi HE, et al. Recent advances in transdermal drug delivery systems: a review. Biomater Res. 2021;25(1):24. doi: 10.1186/s40824-021-00226-6.
  • Sivadasan D. 2024. Design features, quality by design approach, characterization, therapeutic applications and clinical considerations of transdermal therapeutic systems – a comprehensive review.
  • Abbot V, Sharma T, Bhardwaj V, et al. Development, characterization and in vitro antimicrobial evaluation of novel flavonoids entrapped micellar topical formulations of neomycin sulfate. J Pharm Sci. 2022;111(12):3287–3296. doi: 10.1016/j.xphs.2022.08.013.
  • Bellefroid C, Reusch C, Lechanteur A, et al. Systematic study of liposomes composition towards efficient delivery of plasmid DNA as potential application of dermal fibroblasts targeting. Int J Pharm. 2021;593:120122. doi: 10.1016/j.ijpharm.2020.120122.
  • Roberts MS, Mohammed Y, Pastore MN, et al. Topical and cutaneous delivery using nanosystems. J Control Release. 2017;247:86–105. doi: 10.1016/j.jconrel.2016.12.022.
  • Krishnan V, Mitragotri S. Nanoparticles for topical drug delivery: Potential for skin cancer treatment. Adv Drug Deliv Rev. 2020;153:87–108. doi: 10.1016/j.addr.2020.05.011.
  • Fu X, Shi Y, Wang H, et al. Ethosomal gel for improving transdermal delivery of thymosin β-4. Int J Nanomedicine. 2019;14:9275–9284. doi: 10.2147/IJN.S228863.
  • Nasr S, Rady M, Gomaa I, et al. Ethosomes and lipid-coated chitosan nanocarriers for skin delivery of a chlorophyll derivative: a potential treatment of squamous cell carcinoma by photodynamic therapy. Int J Pharm. 2019;568:118528. doi: 10.1016/j.ijpharm.2019.118528.
  • Santos AC, Rodrigues D, Sequeira JAD, et al. Nanotechnological breakthroughs in the development of topical phytocompounds-based formulations. Int J Pharm. 2019;572:118787. doi: 10.1016/j.ijpharm.2019.118787.
  • Kothapalli P, Vasanthan M. Lipid-based nanocarriers for enhanced delivery of plant-derived bioactive molecules: a comprehensive review. Ther Deliv. 2024;15(2):135–155. doi: 10.4155/tde-2023-0116.
  • Alfehaid FS, Nair AB, Shah H, et al. Enhanced transdermal delivery of apremilast loaded ethosomes: Optimization, characterization and in vivo evaluation. J Drug Delivery Sci Technol. 2024;91:105211. doi: 10.1016/j.jddst.2023.105211.
  • Cristiano MC, Froiio F, Spaccapelo R, et al. Sulforaphane-Loaded ultradeformable vesicles as a potential natural nanomedicine for the treatment of skin cancer diseases. Pharmaceutics. 2020;12(1):1–13. doi: 10.3390/pharmaceutics12010006.
  • Verma P, Pathak K. Therapeutic and cosmeceutical potential of ethosomes: an overview. J Adv Pharm Technol Res. 2010;1(3):274–282. doi: 10.4103/0110-5558.72415.
  • Agrawal A, Joshi A, Bhattacharya S. Recent excavation of nanoethosomes in current drug delivery. Curr Drug Deliv. 2024;21(2):168–183. doi: 10.2174/1567201820666221220103013.
  • Touitou E, Dayan N, Bergelson L, et al. Ethosomes - novel vesicular carriers for enhanced delivery: characterization and skin penetration properties. J Control Release. 2000;65(3):403–418. doi: 10.1016/s0168-3659(99)00222-9.
  • Zahid SR, Upmanyu N, Dangi S, et al. Ethosome: a novel vesicular carrier for transdermal drug delivery. J Drug Delivery Ther. 2018;8(6):318–326. doi: 10.22270/jddt.v8i6.2028.
  • Paiva-Santos AC, Silva AL, Guerra C, et al. Ethosomes as nanocarriers for the development of skin delivery formulations. Pharm Res. 2021;38(6):947–970. doi: 10.1007/s11095-021-03053-5.
  • Alshehri S, Hussain A, Altamimi MA, et al. In vitro, ex vivo, and in vivo studies of binary ethosomes for transdermal delivery of acyclovir: a comparative assessment. J Drug Delivery Sci Technol. 2021;62:102390. doi: 10.1016/j.jddst.2021.102390.
  • Aljohani AA, Alanazi MA, Munahhi LA, et al. Binary ethosomes for the enhanced topical delivery and antifungal efficacy of ketoconazole. OpenNano. 2023;11:100145. doi: 10.1016/j.onano.2023.100145.
  • Mohanty D, Mounika A, Bakshi V, et al. Ethosomes: a novel approach for transdermal drug delivery. IJCTR. 2018;11(8):219–226. doi: 10.20902/IJCTR.2018.110826.
  • Wang H, Shao Q, Zhang Y, et al. Preparation and evaluation of liposomes containing ethanol and propylene glycol as carriers for nicotine. Curr Drug Deliv. 2024;21(2):249–260. doi: 10.2174/1567201820666230428122845.
  • Raina N, Rani R, Thakur VK, et al. New insights in topical drug delivery for skin disorders: from a nanotechnological perspective. ACS Omega. 2023;8(22):19145–19167. doi: 10.1021/acsomega.2c08016.
  • Dave V, Kumar D, Lewis S, et al. Ethosome for enhanced transdermal drug delivery of aceclofenac. Int J Drug Delivery. 2010;2(1):81–92. doi: 10.5138/ijdd.2010.0975.0215.02016.
  • Puri R, Jain S. Ethogel topical formulation for increasing the local bioavailability of 5-fluorouracil: a mechanistic study. Anticancer Drugs. 2012;23(9):923–934. doi: 10.1097/CAD.0b013e3283534051.
  • Elnaggar YSR, El-Refaie WM, El-Massik MA, et al. Lecithin-based nanostructured gels for skin delivery: an update on state of art and recent applications. J Control Release. 2014;180(1):10–24. doi: 10.1016/j.jconrel.2014.02.004.
  • Goindi S, Dhatt B, Kaur A. Ethosomes-based topical delivery system of antihistaminic drug for treatment of skin allergies. J Microencapsul. 2014;31(7):716–724. doi: 10.3109/02652048.2014.918667.
  • Paolino D, Lucania G, Mardente D, et al. Ethosomes for skin delivery of ammonium glycyrrhizinate: in vitro percutaneous permeation through human skin and in vivo anti-inflammatory activity on human volunteers. J Control Release. 2005;106(1–2):99–110. doi: 10.1016/j.jconrel.2005.04.007.
  • Ahad A, Aqil M, Kohli K, et al. Enhanced transdermal delivery of an anti-hypertensive agent via nanoethosomes: statistical optimization, characterization and pharmacokinetic assessment. Int J Pharm. 2013;443(1-2):26–38. doi: 10.1016/j.ijpharm.2013.01.011.
  • Godin B, Touitou E. Ethosomes: new prospects in transdermal delivery. Crit Rev Ther Drug Carrier Syst. 2003;20(1):63–102. doi: 10.1615/critrevtherdrugcarriersyst.v20.i1.20.
  • Liu X, Liu H, Liu J, et al. Preparation of a ligustrazine ethosome patch and its evaluation in vitro and in vivo. Int J Nanomedicine. 2011;6:241–247. doi: 10.2147/IJN.S16044.
  • Nagadevi B, Kumar KS, Venkanna P, et al. 2014. Formulation and characterisation of tizanidine hydrochloride loaded ethosomes patch.
  • Shen S, Liu SZ, Zhang YS, et al. Compound antimalarial ethosomal cataplasm: preparation, evaluation, and mechanism of penetration enhancement. Int J Nanomedicine. 2015;10:4239–4253. doi: 10.2147/IJN.S83402.
  • X L, H L, Z Z, et al. Pharmacokinetics of ligustrazine ethosome patch in rats and anti-myocardial ischemia and anti-ischemic reperfusion injury effect. Int J Nanomedicine. 2011;6:1391–1398. doi: 10.2147/IJN.S20263.
  • Kaur CD, Saraf S. Topical vesicular formulations of curcuma longa extract on recuperating the ultraviolet radiation-damaged skin. J Cosmet Dermatol. 2011;10(4):260–265. doi: 10.1111/j.1473-2165.2011.00586.x.
  • Mbah CC, Builders PF, Attama AA. Nanovesicular carriers as alternative drug delivery systems: ethosomes in focus. Expert Opin Drug Deliv. 2014;11(1):45–59. doi: 10.1517/17425247.2013.860130.
  • Abbot V, Sharma D, Attri H, et al. An electrolyte induced thermodynamic study of quercetin and tween 20: a physico-chemical approach for formulation development. Mater Today Proc. 2023. doi: 10.1016/j.matpr.2023.01.239.
  • Yang L, Wu L, Wu D, et al. Mechanism of transdermal permeation promotion of lipophilic drugs by ethosomes. Int J Nanomedicine. 2017;12:3357–3364. doi: 10.2147/IJN.S134708.
  • Siddiqua A, Abdul S, Taha M. A review on novel approach of drug delivery-ethosomes *corresponding author. Siddiqua et al. World J Pharmaceut Res. 2020;9(5):2192.
  • Punith M, Joshi VG, Tejaswini A. Review article a review on ethosomes as recent promising approaches for enhanced transdermal drug delivery system. Int J Pharma Chem Res. 2021;7(1):18–24.
  • Jafari A, Daneshamouz S, Ghasemiyeh P, et al. Ethosomes as dermal/transdermal drug delivery systems: applications, preparation and characterization. J Liposome Res. 2023;33(1):34–52. doi: 10.1080/08982104.2022.2085742.
  • Chettupalli AK, Ajmera S, Amarachinta PR, et al. Quality by design approach for preparation, characterization, and statistical optimization of naproxen sodium-loaded ethosomes via transdermal route. CBC. 2023;19(10):79–98. doi: 10.2174/1573407219666230606142116.
  • Zafar A, Yasir M, Khalid M. Development of piperine-loaded soft binary ethosomal gel to improve transdermal delivery: box-Bhekhen design optimization, ex-vivo permeation, and antimicrobial evaluation. J Clust Sci. 2023;35(1):311–325. doi: 10.1007/s10876-023-02479-8.
  • Zhang M, Zhuang X, Li S, et al. Designed fabrication of Phloretin-Loaded propylene glycol binary ethosomes: Stability, skin permeability and antioxidant activity. Molecules. 2024;29(1):66. doi: 10.3390/molecules29010066.
  • Shabreen MR, Sangeetha S. Ethosomes: a novel drug delivery system and their therapeutic applications-a review. Res J Pharm Technol. 2020;13(4):1972. doi: 10.5958/0974-360X.2020.00355.8.
  • Tyagi S, Chaurasia L, Islam M, et al. A review on novel approach ethosome as a nanocarrier for transdermal drug delivery. Biomedical Research. 2022;33(3):67–73.
  • Das SK, Chakraborty S, Roy C, et al. Ethosomes as novel vesicular carrier: an overview of the principle, preparation and its applications. Curr Drug Deliv. 2018;15(6):795–817. doi: 10.2174/1567201815666180116091604.
  • Godin B, Touitou E. Erythromycin ethosomal systems: physicochemical characterization and enhanced antibacterial activity. Curr Drug Deliv. 2005;2(3):269–275. doi: 10.2174/1567201054367931.
  • Godin B, Touitou E, Rubinstein E, et al. A new approach for treatment of deep skin infections by an ethosomal antibiotic preparation: an in vivo study. J Antimicrob Chemother. 2005;55(6):989–994. doi: 10.1093/jac/dki125.
  • Mohammed MI, Makky AMA, Teaima MHM, et al. Transdermal delivery of vancomycin hydrochloride using combination of nano-ethosomes and iontophoresis: in vitro and in vivo study. Drug Deliv. 2016;23(5):1558–1564. doi: 10.3109/10717544.2015.1013200.
  • Horwitz E, Pisanty S, Czerninski R, et al. A clinical evaluation of a novel liposomal carrier for acyclovir in the topical treatment of recurrent herpes labialis. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 1999;87(6):700–705. doi: 10.1016/s1079-2104(99)70164-2.
  • Padula C, Sartori F, Marra F, et al. The influence of iontophoresis on acyclovir transport and accumulation in rabbit ear skin. Pharm Res. 2005;22(9):1519–1524. doi: 10.1007/s11095-005-5884-1.
  • Worrall G. Topical acyclovir for recurrent herpes labialis in primary care: Critical appraisal. Can Fam Physician. 1991;37:92–98.
  • Bagatell CJ, Bremner WJ. Androgens in men–uses and abuses. N Engl J Med. 1996;334(11):707–714. doi: 10.1056/NEJM199603143341107.
  • Dobs AS, Meikle AW, Arver S, et al. Pharmacokinetics, efficacy, and safety of a permeation-enhanced testosterone transdermal system in comparison with bi-weekly injections of testosterone enanthate for the treatment of hypogonadal men. J Clin Endocrinol Metab. 1999;84(10):3469–3478. doi: 10.1210/jcem.84.10.6078.
  • Zitzmann M, Nieschlag E. Hormone substitution in male hypogonadism. Mol Cell Endocrinol. 2000;161(1–2):73–88. doi: 10.1016/s0303-7207(99)00227-0.
  • Ainbinder D, Touitou E. Testosterone ethosomes for enhanced transdermal delivery. Drug Deliv. 2005;12(5):297–303. doi: 10.1080/10717540500176910.
  • Ohlsen RI, Pilowsky LS. The place of partial agonism in psychiatry: recent developments. J Psychopharmacol. 2016;19(4):408–413. doi: 10.1177/0269881105053308.
  • Tunnicliff G. Molecular basis of buspirone’s anxiolytic action. Pharmacol Toxicol. 1991;69(3):149–156. doi: 10.1111/j.1600-0773.1991.tb01289.x.
  • Mahmood I, Sahajwalla C. Clinical pharmacokinetics and pharmacodynamics of buspirone, an anxiolytic drug. Clin Pharmacokinet. 1999;36(4):277–287. doi: 10.2165/00003088-199936040-00003.
  • Sakr A, Andheria M. A comparative multidose pharmacokinetic study of buspirone extended-release tablets with a reference immediate-release product. J Clin Pharmacol. 2001;41(8):886–894. doi: 10.1177/00912700122010681.
  • Cevc G. Transfersomes, liposomes and other lipid suspensions on the skin: permeation enhancement, vesicle penetration, and transdermal drug delivery. Crit Rev Ther Drug Carrier Syst. 1996;13(3-4):257–388. doi: 10.1615/critrevtherdrugcarriersyst.v13.i3-4.30.
  • Finnin BC, Morgan TM. Transdermal penetration enhancers: applications, limitations, and potential. J Pharm Sci. 1999;88(10):955–958. doi: 10.1021/js990154g.
  • Smith EW, Maibach HI. 1995. Percutaneous penetration enhancers, 500.
  • Tachibana K. Transdermal delivery of insulin to alloxan-diabetic rabbits by ultrasound exposure. Pharm Res. 1992;9(7):952–954. doi: 10.1023/a:1015869420159.
  • Wiley Online Library. Enhanced delivery of drugs into and across the skin by ethosomal carriers - Touitou - 2000 - drug development research. 2023. 10.1002/1098-2299%28200007/08%2950%3A3/4%3C406%3A%3AAID-DDR23%3E3.0.CO%3B2-M
  • Chen JG, Liu YF, Gao TW. Preparation and anti-inflammatory activity of triptolide ethosomes in an erythema model. J Liposome Res. 2010;20(4):297–303. doi: 10.3109/08982100903544144.
  • Lodzki M, Godin B, Rakou L, et al. Cannabidiol-transdermal delivery and anti-inflammatory effect in a murine model. J Control Release. 2003;93(3):377–387. doi: 10.1016/j.jconrel.2003.09.001.
  • Zhao YZ, Lu CT, Zhang Y, et al. Selection of high efficient transdermal lipid vesicle for curcumin skin delivery. Int J Pharm. 2013;454(1):302–309. doi: 10.1016/j.ijpharm.2013.06.052.
  • Mbah C, Builders P, Nzekwe I, et al. Formulation and in vitro evaluation of pH-responsive ethosomes for vaginal delivery of metronidazole. J Drug Delivery Sci Technol. 2014;24(6):565–571. doi: 10.1016/S1773-2247(14)50120-7.
  • Shumilov M, Bercovich R, Duchi S, et al. Ibuprofen transdermal ethosomal gel: characterization and efficiency in animal models. J Biomed Nanotechnol. 2010;6(5):569–576. doi: 10.1166/jbn.2010.1153.
  • Raza K, Singh B, Lohan S, et al. Nano-lipoidal carriers of tretinoin with enhanced percutaneous absorption, photostability, biocompatibility and anti-psoriatic activity. Int J Pharm. 2013;456(1):65–72. doi: 10.1016/j.ijpharm.2013.08.019.
  • Ainbinder D, Touitou E. A new approach for skin tumor treatment: from delivery system characterization to in vivo evaluation. Drug Deliv Transl Res. 2011;1(1):53–65. doi: 10.1007/s13346-010-0006-y.
  • Fang YP, Tsai YH, Wu PC, et al. Comparison of 5-aminolevulinic acid-encapsulated liposome versus ethosome for skin delivery for photodynamic therapy. Int J Pharm. 2008;356(1-2):144–152. doi: 10.1016/j.ijpharm.2008.01.020.
  • Yu X, Du L, Li Y, et al. Improved anti-melanoma effect of a transdermal mitoxantrone ethosome gel. Biomed Pharmacother. 2015;73:6–11. doi: 10.1016/j.biopha.2015.05.002.
  • Ma M, Wang J, Guo F, et al. Development of nanovesicular systems for dermal imiquimod delivery: physicochemical characterization and in vitro/in vivo evaluation. J Mater Sci Mater Med. 2015;26(6):191. doi: 10.1007/s10856-015-5524-1.
  • Coelho FL, Rodembusch FS, Campo LF. Synthesis, characterization and photophysics of new photoactive ESIPT lipophilic dyes. Partition experiments with different composed liposomes. Dyes Pigm. 2014;110:134–142. doi: 10.1016/j.dyepig.2014.04.024.
  • Ahad A, Aqil M, Kohli K, et al. Nano vesicular lipid carriers of angiotensin II receptor blocker: anti-hypertensive and skin toxicity study in focus. Artif Cells Nanomed Biotechnol. 2016;44(3):1002–1007. doi: 10.3109/21691401.2015.1008509.
  • Celia C, Cilurzo F, Trapasso E, et al. Ethosomes® and transfersomes® containing linoleic acid: physicochemical and technological features of topical drug delivery carriers for the potential treatment of melasma disorders. Biomed Microdevices. 2012;14(1):119–130. doi: 10.1007/s10544-011-9590-y.
  • Na B, Choi BW, Kim B. Size and CT density of iodine-containing ethosomal vesicles obtained by membrane extrusion: potential for use as CT contrast agents. Biotechnol J. 2013;8(11):1347–1353. doi: 10.1002/biot.201300110.
  • Nainwal N, Jawla S, Singh R, et al. Transdermal applications of ethosomes–a detailed review. J Liposome Res. 2019;29(2):103–113. doi: 10.1080/08982104.2018.1517160.
  • Rao Y, Zheng F, Zhang X, et al. In vitro percutaneous permeation and skin accumulation of finasteride using vesicular ethosomal carriers. AAPS PharmSciTech. 2008;9(3):860–865. doi: 10.1208/s12249-008-9124-y.
  • Chang LW, Hou ML, Tsai TH. Silymarin in liposomes and ethosomes: pharmacokinetics and tissue distribution in free-moving rats by high-performance liquid chromatography-tandem mass spectrometry. J Agric Food Chem. 2014;62(48):11657–11665. doi: 10.1021/jf504139g.
  • Garg BJ, Garg NK, Beg S, et al. Nanosized ethosomes-based hydrogel formulations of methoxsalen for enhanced topical delivery against vitiligo: Formulation optimization, in vitro evaluation and preclinical assessment. J Drug Target. 2016;24(3):233–246. doi: 10.3109/1061186X.2015.1070855.
  • Yeh MI, Huang HC, Liaw JH, et al. Ethosomes in hair dye products as carriers of the major compounds of black tea extracts. Int J Dermatol. 2013;52(7):868–875. doi: 10.1111/ijd.12014.
  • Dubey V, Mishra D, Nahar M, et al. Enhanced transdermal delivery of an anti-HIV agent via ethanolic liposomes. Nanomedicine. 2010;6(4):590–596. doi: 10.1016/j.nano.2010.01.002.
  • De La Presa P, Rueda T, Morales MDP, et al. Gold nanoparticles generated in ethosome bilayers, as revealed by cryo-electron-tomography. J Phys Chem B. 2009;113(10):3051–3057. doi: 10.1021/jp808650e.
  • Mishra D, Mishra PK, Dabadghao S, et al. Comparative evaluation of hepatitis B surface antigen-loaded elastic liposomes and ethosomes for human dendritic cell uptake and immune response. Nanomedicine. 2010;6(1):110–118. doi: 10.1016/j.nano.2009.04.003.
  • Bendas ER, Tadros MI. Enhanced transdermal delivery of salbutamol sulfate via ethosomes. AAPS PharmSciTech. 2007;8(4):E107. doi: 10.1208/pt0804107.
  • Chourasia MK, Kang L, Chan SY. Nanosized ethosomes bearing ketoprofen for improved transdermal delivery. Results Pharma Sci. 2011;1(1):60–67. doi: 10.1016/j.rinphs.2011.10.002.
  • IJPRS. Formulation and evaluation of liposomal topical gels of linezolid. 2023. Available from: https://www.ijprs.com/article/formulation-and-evaluation-of-liposomal-topical-gels-of-linezolid/
  • Li G, Fan Y, Fan C, et al. Tacrolimus-loaded ethosomes: physicochemical characterization and in vivo evaluation. Eur J Pharm Biopharm. 2012;82(1):49–57. doi: 10.1016/j.ejpb.2012.05.011.
  • Rakesh R, Anoop KR. Formulation and optimization of nano-sized ethosomes for enhanced transdermal delivery of cromolyn sodium. J Pharm Bioallied Sci. 2012;4(4):333–340. doi: 10.4103/0975-7406.103274.
  • Verma DD, Fahr A. Synergistic penetration enhancement effect of ethanol and phospholipids on the topical delivery of cyclosporin a. J Control Release. 2004;97(1):55–66. doi: 10.1016/j.jconrel.2004.02.028.
  • Zhang JP, Wei YH, Zhou Y, et al. Ethosomes, binary ethosomes and transfersomes of terbinafine hydrochloride: a comparative study. Arch Pharm Res. 2012;35(1):109–117. doi: 10.1007/s12272-012-0112-0.
  • Zhou Y, Wei Y, Liu H, et al. Preparation and in vitro evaluation of ethosomal total alkaloids of sophora alopecuroides loaded by a transmembrane pH-gradient method. AAPS PharmSciTech. 2010;11(3):1350–1358. doi: 10.1208/s12249-010-9509-6.
  • Zhu X, Li F, Peng X, et al. Formulation and evaluation of lidocaine base ethosomes for transdermal delivery. Anesth Analg. 2013;117(2):352–357. doi: 10.1213/ANE.0b013e3182937b74.
  • Bodade SS, Shaikh KS, Kamble MS, et al. A study on ethosomes as mode for transdermal delivery of an antidiabetic drug. Drug Deliv. 2013;20(1):40–46. doi: 10.3109/10717544.2012.752420.
  • Shen LN, Zhang YT, Wang Q, et al. Enhanced in vitro and in vivo skin deposition of apigenin delivered using ethosomes. Int J Pharm. 2014;460(1-2):280–288. doi: 10.1016/j.ijpharm.2013.11.017.
  • Ascenso A, Raposo S, Batista C, et al. Development, characterization, and skin delivery studies of related ultradeformable vesicles: transfersomes, ethosomes, and transethosomes. Int J Nanomedicine. 2015;10:5837–5851. doi: 10.2147/IJN.S86186.
  • Heydari S, Ghanbarzadeh S, Anoush B, et al. Nanoethosomal formulation of gammaoryzanol for skin-aging protection and wrinkle improvement: a histopathological study. Drug Dev Ind Pharm. 2017;43(7):1154–1162. doi: 10.1080/03639045.2017.1300169.
  • Limsuwan T, Boonme P, Amnuaikit T. The optimized HPLC method for quantitative analysis of phenylethyl resorcinol loaded in the novel vesicle carriers and permeated in in vitro skin permeation study. J Chromatogr Sci. 2017;55(10):992–999. doi: 10.1093/chromsci/bmx063.
  • Park SN, Lee HJ, Gu HA. Enhanced skin delivery and characterization of rutin-loaded ethosomes. Korean J Chem Eng. 2014;31(3):485–489. doi: 10.1007/s11814-013-0232-3.
  • Salmannejad F, Ghari T, Toliyat T. Formulation and evaluation of ethosomes for transdermal delivery of clonazepam. Int J Biosci (IJB). 2015;6(5):308–316.
  • Gupta P, Hafeez A, Kushwaha P. Development and evaluation of topical ethosomal gel for fungal infections. Drug Res (Stuttg)). 2023;73(1):46–53. doi: 10.1055/a-1924-7818.
  • Verma DD, Verma S, Blume G, et al. Liposomes increase skin penetration of entrapped and non-entrapped hydrophilic substances into human skin: a skin penetration and confocal laser scanning microscopy study. Eur J Pharm Biopharm. 2003;55(3):271–277. doi: 10.1016/s0939-6411(03)00021-3.
  • Shinde P, Page A, Bhattacharya S. Ethosomes and their monotonous effects on skin cancer disruption. Front Nanotechnol. 2023;5:1–21. doi: 10.3389/fnano.2023.1087413.
  • Fang JY, Hwang TL, Leu YL. Effect of enhancers and retarders on percutaneous absorption of flurbiprofen from hydrogels. Int J Pharm. 2003;250(2):313–325. doi: 10.1016/s0378-5173(02)00540-9.
  • Ciolacu DE, Nicu R, Ciolacu F. Cellulose-Based hydrogels as sustained drug-delivery systems. Materials. 2020;13(22):5270. doi: 10.3390/ma13225270.
  • Jain S, Umamaheshwari RB, Bhadra D, et al. Ethosomes : a novel vesicular carrier for enhanced transdermal delivery of an AntiHIV agent. Indian J Pharmaceut Sci. n.d.;66(1):72.
  • Kaur G, Grewal J, Jyoti K, et al. Oral controlled and sustained drug delivery systems: Concepts, advances, preclinical, and clinical status. Drug Target Stimuli Sensit Drug Deliv Syst. 2018:567–626.
  • Song CK, Balakrishnan P, Shim CK, et al. A novel vesicular carrier, transethosome, for enhanced skin delivery of voriconazole: Characterization and in vitro/in vivo evaluation. Colloids Surf B Biointerfaces. 2012;92:299–304. doi: 10.1016/j.colsurfb.2011.12.004.
  • Sudhakar CK, Upadhyay N, Jain S, et al. Ethosomes as non-invasive loom for transdermal drug delivery system. Nanomedicine and Drug Delivery. 2012;1:1–16.
  • Verma P, Yadav AK, Gupta D, et al. European journal of review on ethosomes : a novel drug delivery system. European Journal of Pharmaceutical and Medical Research. 2023;10(2):147–159.
  • Nasr AM, Moftah F, Abourehab MAS. 2022. Design, formulation, and characterization of valsartan nanoethosomes for improving their bioavailability.
  • Akhtar N, Akhtar N, Menaa F, et al. Fabrication of ethosomes containing tocopherol acetate to enhance transdermal permeation: in vitro and ex vivo characterizations. Gels. 2022;8(6):1–18. doi: 10.3390/gels8060335.
  • Huanbutta K, Rattanachitthawat N, Luangpraditkun K, et al. Development and evaluation of ethosomes loaded with zingiber zerumbet linn rhizome extract for antifungal skin infection in deep layer skin. Pharmaceutics. 2022;14(12):2765. doi: 10.3390/pharmaceutics14122765.
  • Esposito E, Calderan L, Galvan A, et al. Ex vivo evaluation of ethosomes and transethosomes applied on human skin: a comparative study. Int J Mol Sci. 2022;23(23):15112. doi: 10.3390/ijms232315112.
  • El-Hashemy HA. Design, formulation and optimization of topical ethosomes using full factorial design: in-vitro and ex-vivo characterization. J Liposome Res. 2022;32(1):74–82. doi: 10.1080/08982104.2021.1955925.
  • Moideen Muthu Mohamed J, Khan BA, Rajendran V, et al. Polymeric ethosomal gel loaded with nimodipine: Optimisation, pharmacokinetic and histopathological analysis. Saudi Pharm J. 2022;30(11):1603–1611. doi: 10.1016/j.jsps.2022.09.003.
  • Zaki RM, Seshadri VD, Mutayran AS, et al. Wound healing efficacy of rosuvastatin transethosomal gel, I optimal optimization, histological and in vivo evaluation. Pharmaceutics. 2022;14(11):2521. doi: 10.3390/pharmaceutics14112521.
  • Ahmed TA, Alzahrani MM, Sirwi A, et al. The antifungal and ocular permeation of ketoconazole from ophthalmic formulations containing trans-ethosomes nanoparticles. Pharmaceutics. 2021;13(2):151. doi: 10.3390/pharmaceutics13020151.
  • Hajare AA, Dol HS. Screening of effective formulation techniques for designing and fabrication of terbinafine hydrochloride ethosomes. Res J Pharm Technol. 2021;14(3):1353–1359. doi: 10.5958/0974-360X.2021.00241.9.
  • Ammar HO, Tadros MI, Salama NM, et al. Ethosome-derived invasomes as a potential transdermal delivery system for vardenafil hydrochloride: Development, optimization and application of physiologically based pharmacokinetic modeling in adults and geriatrics. Int J Nanomedicine. 2020;15:5671–5685. doi: 10.2147/IJN.S261764.
  • Dave V, Bhardwaj N, Gupta N, et al. Herbal ethosomal gel containing luliconazole for productive relevance in the field of biomedicine. 3 Biotech. 2020;10(3):97. doi: 10.1007/s13205-020-2083-z.
  • Shukla R, Tiwari G, Tiwari R, et al. Formulation and evaluation of the topical ethosomal gel of melatonin to prevent UV radiation. J Cosmet Dermatol. 2020;19(8):2093–2104. doi: 10.1111/jocd.13251.
  • Arora D, Nanda S. Quality by design driven development of resveratrol loaded ethosomal hydrogel for improved dermatological benefits via enhanced skin permeation and retention. Int J Pharm. 2019;567:118448. doi: 10.1016/j.ijpharm.2019.118448.
  • Mahmood S, Mandal UK, Chatterjee B. Transdermal delivery of raloxifene HCl via ethosomal system: Formulation, advanced characterizations and pharmacokinetic evaluation. Int J Pharm. 2018;542(1–2):36–46. doi: 10.1016/j.ijpharm.2018.02.044.
  • Abdel Messih HA, Ishak RAH, Geneidi AS, et al. Nanoethosomes for transdermal delivery of tropisetron HCl: multi-factorial predictive modeling, characterization, and ex vivo skin permeation. Drug Dev Ind Pharm. 2017;43(6):958–971. doi: 10.1080/03639045.2017.1287717.
  • Chen ZX, Li B, Liu T, et al. Evaluation of paeonol-loaded transethosomes as transdermal delivery carriers. Eur J Pharm Sci. 2017;99:240–245. doi: 10.1016/j.ejps.2016.12.026.
  • Ahmed TA, El-Say KM, Aljaeid BM, et al. Transdermal glimepiride delivery system based on optimized ethosomal nano-vesicles: Preparation, characterization, in vitro, ex vivo and clinical evaluation. Int J Pharm. 2016;500(1-2):245–254. doi: 10.1016/j.ijpharm.2016.01.017.
  • Mao YT, Wang Y, Chen XX, et al. Ethosomes as delivery system for transdermal administration of vinpocetine - PubMed [online]. 2023. Available from: https://pubmed.ncbi.nlm.nih.gov/23802438/
  • Bhosale SS, Avachat AM. Design and development of ethosomal transdermal drug delivery system of valsartan with preclinical assessment in wistar albino rats. J Liposome Res. 2013;23(2):119–125. doi: 10.3109/08982104.2012.753457.
  • Mishra AD, Khunt DM, Ghayal AH, et al. Formulation and optimization of ethosomes for transdermal delivery of felodipine. Res J Pharmacy Technol. 2012;5(12):1509–1517.
  • Maurya SD, Prajapati SK, Gupta AK, et al. Formulation development and evaluation of ethosome of stavudine. Indian J Pharmaceut Educ Res. 2010;44(1):102–108.
  • Suma US, Parthiban S, Senthil Kumar GP. Formulation and evaluation of niosomal gel for transdermal delivery of lamivudine. World J Pharm Res. 2016;5(5):1332–1342.
  • Touitou E, Dayan N, Touitou E. Carriers for skin delivery of trihexyphenidyl HCl : Ethosomes vs liposomes carriers for skin delivery of trihexyphenidyl HCl : ethosomes vs. liposomes. Biomaterials. 2015;21(18):1879–1885. doi: 10.1016/s0142-9612(00)00063-6.

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