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Research Article

Validation of JCountPro software for efficient assessment of ionizing radiation-induced foci in human lymphocytes

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Pages 766-773 | Received 16 Mar 2016, Accepted 31 Jul 2016, Published online: 20 Sep 2016

References

  • Adams G, Martin OA, Roos DE, Lobachevsky PN, Potter AE, Zacest AC, Bezak E, Bonner WM, Martin RF, Leong T. 2012. Enhanced intrinsic radiosensitivity after treatment with stereotactic radiosurgery for an acoustic neuroma. Radiother Oncol. 103:410–414.
  • Barber P, Locke R, Pierce G, Rothkamm K, Vojnovic B. 2007. Gamma-H2AX foci counting: image processing and control software for high-content screening: Biomedical Optics (BiOS) 2007:64411M–64411M-10.
  • Barnard S, Ainsbury E, Al-Hafidh J, Hadjidekova V, Hristova R, Lindholm C, Gil OM, Moquet J, Moreno M, Rößler U. 2014. The first gamma-H2AX biodosimetry intercomparison exercise of the developing European biodosimetry network RENEB. Radiat Protect Dosimetry. ncu259.
  • Bekker-Jensen S, Lukas C, Kitagawa R, Melander F, Kastan MB, Bartek J, Lukas J. 2006. Spatial organization of the mammalian genome surveillance machinery in response to DNA strand breaks. J Cell Biol. 173:195–206.
  • Belyaev IY. 2010. Radiation-induced DNA repair foci: spatio-temporal aspects of formation, application for assessment of radiosensitivity and biological dosimetry. Mutat Res. 704:132–141.
  • Bocker W, Iliakis G. 2006. Computational methods for analysis of foci: validation for radiation-induced gamma-H2AX foci in human cells. Radiat Res. 165:113–124.
  • Brand M, Sommer M, Achenbach S, Anders K, Lell M, Löbrich M, Uder M, Kuefner MA. 2012. X-ray induced DNA double-strand breaks in coronary CT angiography: comparison of sequential, low-pitch helical and high-pitch helical data acquisition. Eur J Radiol. 81:e357–362.
  • Depuydt J, Baert A, Vandersickel V, Thierens H, Vral A. 2013. Relative biological effectiveness of mammography X-rays at the level of DNA and chromosomes in lymphocytes. Int J Radiat Biol. 89:532–538.
  • Durdik M, Kosik P, Gursky J, Vokalova L, Markova E, Belyaev I. 2015. Imaging flow cytometry as a sensitive tool to detect low-dose-induced DNA damage by analyzing 53BP1 and gammaH2AX foci in human lymphocytes. Cytometry A. 4:227–231.
  • Ghardi M, Moreels M, Chatelain B, Chatelain C, Baatout S. 2012. Radiation-induced double strand breaks and subsequent apoptotic DNA fragmentation in human peripheral blood mononuclear cells. Int J Molec Med. 29:769–780.
  • Groesser T, Fontenay GV, Han J, Chang H, Pluth J, Parvin B. 2015. Quantification of the dynamics of DNA repair to ionizing radiation via colocalization of 53BP1 and gamma H2AX. In: Bhanu B, Talbot P, editors. Video bioinformatics: from live imaging to knowledge: Computational Biology Series, v. 22: Dordrecht: Springer. p. 253–263.
  • Horn S, Barnard S, Rothkamm K. 2011. Gamma-H2AX-based dose estimation for whole and partial body radiation exposure. PLoS One. 6:e25113.
  • Hou Y-N, Lavaf A, Huang D, Peters S, Huq R, Friedrich V, Rosenstein BS, Kao J. 2009. Development of an automated γ-H2AX immunocytochemistry assay. Radiat Res. 171:360–367.
  • Ivashkevich AN, Martin OA, Smith AJ, Redon CE, Bonner WM, Martin RF, Lobachevsky PN. 2011. gammaH2AX foci as a measure of DNA damage: a computational approach to automatic analysis. Mutat Res. 711:49–60.
  • Kuefner MA, Brand M, Engert C, Kappey H, Uder M, Distel LV. 2013. The effect of calyculin A on the dephosphorylation of the histone gamma-H2AX after formation of X-ray-induced DNA double-strand breaks in human blood lymphocytes. Int J Radiat Biol. 89:424–432.
  • Lamkowski A, Forcheron F, Agay D, Ahmed EA, Drouet M, Meineke V, Scherthan H. 2014. DNA damage focus analysis in blood samples of minipigs reveals acute partial body irradiation. Plos One. 9:e87458.
  • Lobachevsky P, Woodbine L, Hsiao KC, Choo S, Fraser C, Gray P, Smith J, Best N, Munforte L, Korneeva E, et al. 2015. Evaluation of severe combined immunodeficiency and combined immunodeficiency pediatric patients on the basis of cellular radiosensitivity. J Mol Diagn. 17:560–575.
  • Löbrich M, Rief N, Kuhne M, Heckmann M, Fleckenstein J, Rube C, Uder M. 2005. In vivo formation and repair of DNA double-strand breaks after computed tomography examinations. Proc Natl Acad Sci USA. 102:8984–8989.
  • Markova E, Schultz N, Belyaev IY. 2007. Kinetics and dose-response of residual 53BP1/gamma-H2AX foci: co-localization, relationship with DSB repair and clonogenic survival. Int J Radiat Biol. 83:319–329.
  • Markova E, Torudd J, Belyaev I. 2011. Long time persistence of residual 53BP1/gamma-H2AX foci in human lymphocytes in relationship to apoptosis, chromatin condensation and biological dosimetry. Int J Radiat Biol. 87:736–745.
  • Martin OA, Ivashkevich A, Choo S, Woodbine L, Jeggo PA, Martin RF, Lobachevsky P. 2013. Statistical analysis of kinetics, distribution and co-localisation of DNA repair foci in irradiated cells: cell cycle effect and implications for prediction of radiosensitivity. DNA Repair (Amst). 12:844–855.
  • Mognato M, Girardi C, Fabris S, Celotti L. 2009. DNA repair in modeled microgravity: double strand break rejoining activity in human lymphocytes irradiated with gamma-rays. Mutat Res. 663:32–39.
  • Qvarnstrom OF, Simonsson M, Johansson KA, Nyman J, Turesson I. 2004. DNA double strand break quantification in skin biopsies. Radiother Oncol. 72:311–317.
  • Redon CE, Dickey JS, Bonner WM, Sedelnikova OA. 2009. [gamma]-H2AX as a biomarker of DNA damage induced by ionizing radiation in human peripheral blood lymphocytes and artificial skin. Adv Space Res. 43:1171–1178.
  • Roch-Lefevre S, Mandina T, Voisin P, Gaetan G, Mesa JE, Valente M, Bonnesoeur P, Garcia O, Voisin P, Roy L. 2010. Quantification of gamma-H2AX foci in human lymphocytes: a method for biological dosimetry after ionizing radiation exposure. Radiat Res. 174:185–194.
  • Rogakou EP, Boon C, Redon C, Bonner WM. 1999. Megabase chromatin domains involved in DNA double-strand breaks in vivo. J Cell Biol. 146:905–916.
  • Rothkamm K, Balroop S, Shekhdar J, Fernie P, Goh V. 2007. Leukocyte DNA damage after multi-detector row CT: a quantitative biomarker of low-level radiation exposure. Radiology. 242:244–251.
  • Rothkamm K, Löbrich M. 2003. Evidence for a lack of DNA double-strand break repair in human cells exposed to very low X-ray doses. Proc Natl Acad Sci USA. 100:5057–5062.
  • Rube CE, Fricke A, Widmann TA, Furst T, Madry H, Pfreundschuh M, Rube C. 2011. Accumulation of DNA damage in hematopoietic stem and progenitor cells during human aging. PLoS One. 6:e17487.
  • Sak A, Grehl S, Erichsen P, Engelhard M, Grannass A, Levegrun S, Pottgen C, Groneberg M, Stuschke M. 2007. gamma-H2AX foci formation in peripheral blood lymphocytes of tumor patients after local radiotherapy to different sites of the body: dependence on the dose-distribution, irradiated site and time from start of treatment. Int J Radiat Biol. 83:639–652.
  • Scherthan H, Hieber L, Braselmann H, Meineke V, Zitzelsberger H. 2008. Accumulation of DSBs in gamma-H2AX domains fuel chromosomal aberrations. Biochem Biophys Res Commun. 371:694–697.
  • Sorokina S, Markova E, Gursky J, Dobrovodsky J, Belyaev I. 2013. Relative biological efficiency of protons at low and therapeutic doses in induction of 53BP1/gammaH2AX foci in lymphocytes from umbilical cord blood. Int J Radiat Biol. 89:716–723.
  • Turner HC, Sharma P, Perrier JR, Bertucci A, Smilenov L, Johnson G, Taveras M, Brenner DJ, Garty G. 2014. The RABiT: high-throughput technology for assessing global DSB repair. Radiat Environ Biophys. 53:265–272.
  • Van Droogenbroeck M, Talbot H. 1996. Fast computation of morphological operations with arbitrary structuring elements. Pattern Recog Lett. 17:1451–1460.
  • Vandevoorde C, Gomolka M, Roessler U, Samaga D, Lindholm C, Fernet M, Hall J, Pernot E, El-Saghire H, Baatout S, et al. 2015. EPI-CT: in vitro assessment of the applicability of the gamma-H2AX-foci assay as cellular biomarker for exposure in a multicentre study of children in diagnostic radiology. Int J Radiat Biol. 91:653–663.
  • Vasilyev SA, Kubes M, Markova E, Belyaev I. 2013. DNA damage response in CD133 + stem/progenitor cells from umbilical cord blood: low level of endogenous foci and high recruitment of 53BP1. Int J Radiat Biol. 89:301–309.
  • Viau M, Testard I, Shim G, Morat L, Normil MD, Hempel WM, Sabatier L. 2015. Global quantification of γH2AX as a triage tool for the rapid estimation of received dose in the event of accidental radiation exposure. Mutat Res/Genet Toxicol Environ Mutagenesis. 793:123–131.
  • Vincent L. 1993. Morphological grayscale reconstruction in image analysis: applications and efficient algorithms. IEEE Transact Image Process. 2:176–201.
  • Werbrouck J, De Ruyck K, Beels L, Vral A, Van Eijkeren M, De Neve W, Thierens H. 2010. Prediction of late normal tissue complications in RT treated gynaecological cancer patients: potential of the Оі-H2AX foci assay and association with chromosomal radiosensitivity. Oncol Rep. 23:571–578.
  • Wojewodzka M, Sommer S, Kruszewski M, Sikorska K, Lewicki M, Lisowska H, Wegierek-Ciuk A, Kowalska M, Lankoff A. 2015. Defining blood processing parameters for optimal detection of gamma-H2AX foci: a small blood volume method. Radiat Res. 184:95–104.

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