5
Views
4
CrossRef citations to date
0
Altmetric
Original Article

The in Vitro Radiosensitivity of Lymphocytes from Chronic Lymphocytic Leukaemia Using the Differential Staining Cytotoxicity (DiSC) Assay. I—Investigation of the Method

&
Pages 103-110 | Received 08 Feb 1991, Accepted 10 Jun 1991, Published online: 03 Jul 2009

References

  • Bird M.C., Bosanquet A.G., Gilby E.D. In vitro determination of tumour chemosensitivity in haematological malignancies. Hematological Oncology 1985; 3: 1–10
  • Bird M.C., Bosanquet A.G., Forskitt S., Gilby E.D. Semi micro adaptation of a four day differential staining cytotoxicity (DiSC) assay for determining the in vitro chemosensitivity of haematological malignancies. Leukemia Research 1986; 10: 445–449
  • Bird M.C., Godwin V.A.J., Antrobus J.H., Bosanquet A.G. Comparison of in vitro drug sensitivity by the differential staining cytotoxicity (DiSC) and colony-forming assays. British Journal of Cancer 1987; 55: 429–431
  • Bird M.C., Bosanquet A.G., Forskitt S., Gilby E.D. Long term comparison of results of a drug sensitivity assay in vitro with patient response in lymphatic neoplasms. Cancer 1988; 61: 1104–1109
  • Bosanquet A.G. Correlations between therapeutic response of leukaemias and in-vitro drug-sensitivity assay. Lancet 1991; 337: 711–714
  • Bosanquet A.G., Bird M.C., Price W.J.P., Gilby E.D. An assessment of a short term tumour chemosensitivity assay in CLL. British Journal of Cancer 1983; 47: 781–789
  • Boullier B.A., Bosanquet A.G. Reduced cell viability in vitro following exposure to a routine laboratory disinfectant: a cautionary note. Cytotechnology 1990; 4: 109–110
  • Carmichael J., Degraff W.G., Gazdar A.F., Minna J.D., Mitchell J.B. Evaluation of a tetrazolium-based semiautomated colorimetric assay—assessment in chemosensitivity testing. Cancer Research 1987a; 47: 936–942
  • Carmichael J., Degraff W.G., Gazdar A.F., Minna J.D., Mitchell J.B. Evaluation of a tetrazolium-based semiautomated colorimetric assay—assessment in radiosensitivity testing. Cancer Research 1987b; 47: 943–946
  • Hinkley H.J., Bosanquet A.G. The in vitro radiosensitivity of lymphocytes from chronic lymphocytic leukaemia using the differential staining cytotoxicity (DiSC) assay. II. Results on 40 patients. International Journal of Radiation Biology 1992; 61: 111–121
  • Kurohara W., Colman M., Nagourney R.A., Weisenthal L.M., Swingle K., Redpath J.L. Radiation response of cells from human tumour biopsies as assessed by a dye exclusion technique: a possible predictive assay. International Journal of Radiation Biology 1989; 56: 767–770
  • Malaise E.P., Fertil B., Deschavanne P.J., Chavaudra N., Brock W.A. Initial slope of radiation survival curves is characteristic of the origin of primary and established cultures of human tumour cells and fibroblasts. Radiation Research 1987; 111: 319–333
  • MRC. Second Trial on Chronic Lymphocytic Leukaemia Protocol, D. Catovsky. MRC, London 1984
  • Pieters R., Huismans D.R., Leyva A., Veerman A.J.P. Comparison of a rapid automated tetrazolium based (MTT)-assay with a dye exclusion assay for chemosensitivity testing in childhood leukaemia. British Journal of Cancer 1989; 59: 217–220
  • Schrek R., Friedman I.A., Leithold S.L. Variability of the in vitro sensitivity of human leukaemic lymphocytes to X-rays and chemotherapeutic agents. Journal of the National Cancer Institute 1958; 20: 1037–1050
  • Schrek R., Stefani S. Radioresistance of PHA treated normal and leukaemic lymphocytes. Journal of the National Cancer Institute 1964; 32: 507–521
  • Schrek R., Leithold S., Friedman I., Best W. Clinical evaluation of an in vitro test for the radiosensitivity of leukaemic lymphocytes. Blood 1962; 20: 432–442
  • Thomson A.E.R., Vaughan-Smith S., Peel W.E., Wetherley-Mein G. The intrinsic radiosensitivity of lymphocytes in chronic lymphocytic leukaemia, quantitatively determined independently of cell death rate factors. International Journal of Radiation Biology 1985; 48: 943–961
  • Twentyman P.R., Fox N.E., Rees J.K.H. Chemosensitivity testing of fresh leukaemia cells using the MTT colorimetric assay. British Journal of Haematology 1989; 71: 19–24
  • Veerman A.J.P., Pieters R. Drug sensitivity assays in leukaemia and lymphoma. British Journal of Haematology 1990; 74: 381–384
  • Weisenthal L.M., Lippman M.E. Clonogenic and non clonogenic in vitro chemosensitivity assays. Cancer Treatment Reports 1985; 69: 615–632
  • Weisenthal L.M., Dill P.L., Kurnick N.B., Lippman M.E. Comparison of dye exclusion assays with a clonogenic assay in the determination of drug-induced cytotoxicity. Cancer Research 1983; 43: 258–264

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.