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Molecular Membrane Biology Volume 19, 2002 - Issue 2
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Research Article

Gap junctions: structure and function (Review)

W. Howard Evans Departments of Medical Biochemistry & Diagnostic Radiology, University of Wales College of Medicine, Heath Park, Cardiff CF14 4XN, Wales, UK
&
Patricia E. M. Martin Departments of Medical Biochemistry & Diagnostic Radiology, University of Wales College of Medicine, Heath Park, Cardiff CF14 4XN, Wales, UK
Pages 121-136 | Published online: 09 Jul 2009

  • Ahmad, S. and Evans, W. H., 2002, Post-translation integration and oligomerisation of connexin 26 in plasma membranes and evidence of the formation of membrane pores. Implications for the assembly of gap junctions. Biochemical Journal, in press.
  • Ahmad, S., Diez, J. A., George, C. H. and Evans, W. H., 1999, Synthesis and assembly of connexins in vitro into homomeric and heteromerb functional gap junction hemichannels. Biochemical Journal, 339, 247-253.
  • Ahmad, S., Martin. P. E. M. and Evans, W. H., 2001, Assembly of gap junction channels: mechanism, effects of calmodulh antagonists and identification of connexin oligomerizatjon determinants. European Journal of Biochemistry, 268, 4544-4552.
  • Ai, Z., Fischer, A., Spray, D. C., Brown, A. M. and Fishman, G. I., 2000, Wnt-1 regulation of connexin43 in cardiac myocytes. Journal of Clinical Investigations, 105, 161-171.
  • Anumonwo, J. M., Taffet, S. M., Gu, H, Chanson, M, Moreno, A. P. and Delmar, M., 2001, The carboxyl terminal domain regulates the unitary conductance and voltage dependence of connexin40 gap junction channels. Circulation Research, 88, 666-673.
  • Azzam, E. I., de Toledo, S. M. and Little, J. B., 2001, Direct evidence for the participation of gap junction-mediated intercellular communication in the transmission of damage signals from alphaparticle irradiated to nonirradiated cells. Proceedings of the National Academy of Sciences (USA), 98, 473-478.
  • Bailce-Gordon, R. J., Bone, L. J. and Scherer, S. S., 1998, Functional gap junctions in the Schwann cell myelin sheath. Journal of Cell Biology, 142, 1095-1104.
  • Beardslee, M. A., Lerner, D. L., Tadros, P. N., Laing, J. G., Beyer, E. C., Yamada, K. A., Kleber, A. G., Schuessler, R. B. and Saffitz, J. E., 2000, Dephosphorylation and intracellular redistribution of ventricular connexin43 during electrical uncoupling induced by ischemia. Circulation Research, 87, 656-662.
  • Ben nett, M. V., 1997, Gap junctions as electrical synapses. Journal of Neurocytology, 26, 349-366.
  • Bennett, M. V., Barrio, L. C., Bargiello, T. A., Spray, D. C., Hertzberg, E. and Saez, J. C., 1991, Gap junctions: new tools, new answers, new questions. Neuron, 6, 305-320.
  • Bennett, M. V., Zheng, X. and Sogin, M. L., 1994, The connexins and their family tree. Society of General Physiology Series. 49, 223233.
  • Bergoffen, J., Scherer, S. S., Wang, S., Oronzi Scott, M., Bone, L. J., Paul, D. L., Chen, K., Lensch, M. W., Chance, P. F. and Fischbeck, K. H., 1993, Connexin mutations in X-linked CharcotMarie-Tooth disease. Science, 262, 2039-2042.
  • Berman, R. S., Martin, P. E., Evans, W. H. and Griffith, T. M., 2002, Relative contributions of NO and gap functional communication to endothelium-dependart relaxations of rabbit resistance arteries vary with vessel size. Microvascular Research, 63, 115-128.
  • Bevans, C. G. and Harris, A. L., 1999, Direct high affinity modulation of connexin channel activity by cyclic nucleotides. Journal of Biological Chemistry, 274, 3720-3725.
  • Bevilacqua, L. M., Simon, A. M., Maguire, C. T., Gerhrmann, J., Wakimoto, H., Paul, D. L. and Bern I, C. I., 2000, A targeted disruption in connexin 40 leads to distinct atrioventricular conduction defects. Journal of Investigative Cardiac Electrophysiology, 4, 459-467.
  • Boitano, S., Dirksen, E. R. and Sanderson, M. J., 1992, Intercellular propagation of calcium waves mediated by inositol trisphosphate. Science, 258, 292-295.
  • Brosnan, C. F., Scemes, E. and Spray, D. C., 2001, Cytokine regulation of gap junction connectivity: and open-and-shut case or changing partners at the Nexus? American Journal of Pathology, 158, 1565-1569.
  • Bruzzone, S., Guida, L., Zocchi, E. and DeFlora, A., 2001, Connexin 43 hemichanneb mediate Ca -regulated transmembrane NAD fluxes in intact cells. FASEB Journal, 15, 10-12.
  • Bukauskas, F. F., Jordan, K., Bukauskiene, A., Bennett, M. V., Lampe, P. D., Laird, D. W. and Verselis, V. K., 2000, Clustering of connexin 43-enhanced green fluorescent protein gap junction channels and functional coupling in living cells. Proceedings of the National Academy of Sciences (USA). 97, 2556-2561.
  • Cancelas, J. A., Koevoet, W. L. de Koning, A. E., Mayen, A. E., Rombouts, E. J. and Ploemacher, R. E., 2000, Connexin-43 gap junctions are involved in multi-connexin-expressing stromal support of hemopoietc progenitors and stem cells. Blood, 96, 498-505.
  • Cao, F., Eckert, R., Elfgang, C., Nitsche, J. M., Snyder, S. A., Willecke, D. K. and Nicholson, B. J., 1998, A quantitative analysis of connexin-speciflc permeability difference junctions expressed in HeLa transfectants and Xenopus oocytes. Journal of Cell Science, 111,31-43.
  • Castro, C., Gomez-Hemandez, J. M., Silander, K. and Barrio, L. C., 1999, Altered formation of hemichannels and gap junction channels caused by C-terminal connexin-32 mutations. Journal of Neuroscience, 19, 3752-3760.
  • Chanson, M., Berclaz, P. Y., Seem, I., Dudez, T., Wenke-Dollries, K., Pizurki, L., Pavirani, A., Fiedler, M. A. and Suter, S., 2001, Regulation of gap junctional communication by a pro-inflammatory cytokine in cystic fibrosis transmembrane conductance regulatorexpressing but not cystic fibrosis airway cells. American Journal of Pathology, 158, 1775-1784.
  • Chaytor, A. T., Martin, P. E., Edwards, D. H. and Griffith, T. M., 2001, Gap junctional communication underpins EDHF-type relaxations evoked by acetyl choline in the rat hepatic artery. American Journal of Physiology, 280, H2441-H2450.
  • Cotrina, M. L., Lin, J. H., Lopez-Garaa, J. C., Naus, C. C. and Nedergaard, M., 2000, ATP-mediated g lia signaling. Journal of Neuroscience, 20, 2835-2844.
  • Cottrell, G. T. and Burt, J. M., 2001, Heterotypic gap junction channel formation between heteromerb and homomeric Cx40 and Cx43 connexons. American Journal of Physiology, 281, C1559-C1667.
  • Cruciani, V. and Mikalsen, S. O., 1999, Stimulated phosphorylation of intracellular connexin43. Experimental Cell Research, 251, 285-298.
  • Dasgupta, C., Martinez, A.-M., Zuppan, C. W., Shah, M. M., Bailey, L. L. and Reicher, W. H., 2001, Identification of connexin 43 gap junction mutations in patients with hypoplastic left heart syndrome by denaturing gradient gel electrophoresis. Mutation Research, 479, 173-186.
  • Deschenes, S. M., Walcott, J. L., Wexler, T. L., Scherer, S. S. and Fishchbeck, K. H., 1997, Altered trafficking of mutant connexin 32. Journal of Neuroscience, 17, 9077-9084.
  • Dhein, S., 1998, Cardiac gap junctions. Physiology, regulation, pathophysiology and pharmacology (Basel: Karger).
  • Di, W. L., Rugg, E. L., Leigh, I. M. and Kelsell, D. P., 2001, Multiple epidermal connexins are expressed in different keratinocyte subpopulations including connexin 31. Journal of Investigative Dermatology. 117, 958-964.
  • Diez, J. A., Ahmad, S. and Evans, W. H., 1999, Assembly of heteromeric connexons in guinea-pig liver en route to the GoIgI apparatus, plasma membrane and gap junctions. European Journal of Biochemistry, 262, 142-148.
  • Dora, K. A., 2001, Intercellular Ca2+ signalling: the artery wall. Seminars in Cell Development Biology, 12, 27-35.
  • Dupont, E., Ko, Y. S., Rothery, S., Coppen, S. R., Baghai, M., Haw, M. and Severs, N. J., 2001, The gap-junctional protein connexin40 is elevated in patients susceptible to postoperative atrial fibrillation. Circulation, 103, 842-849.
  • Elfgang, C., Eckert, R., Lichtenberg-Frate, H., Butterweck, A., Traub, I., Klein, R. A., Husler, D. F. and Willecke, K., 1995, Specific permeability and selective formation of gap junction channels in connexin-transfected HeLa cells. Journal of Cell Biology, 129, 805-817.
  • Evans, W. H. and Boitano, S., 2001, Connexin mimetic peptides: specific inhibitors of gap-junctional intercellular communication. Biochemical Society Transactions, 29, 606-612.
  • Evert, M. Ott, T., Temme, A., Willecke, K. and Dombrowski, F., 2002, Morphology and morphometric investigation of hepatocel-lular preneoplastic lesions and neoplasms in connexin32-deficient mice. Carcinogenesis, in press.
  • FaIk, M. M., 2000, Connexin-speafic distribution within gap junctions revealed in living cells. Journal of Cell Science. 113,4109-4120.
  • FaIk, M. M., Buehler, L. K., Kumar, N. M. and GiIuIa, N. B., 1997, Cell-free synthesis and assembly of connexins into functional gap junction membrane channels. EMBO Journal, 16, 2703-2716.
  • Foote, C. I., Zhou, L., Zhu, X. and Nicholson, B. J., 1998, The pattern of disulfide linkages in the extracellular loop regions of connexin 32 suggests a model for the docking interlace of gap junctions. Journal of Cell Biology, 140, 1187-1197.
  • Fujimoto, K., Nagafuchi, A., Tsukita, S. K. A., Ohokuma, A. and Shibata, Y., 1997, Dynamics of connexins, E-cadherin and acatenin on cell membranes during gap junction formation. Journal of Cell Science, 110, 311-322.
  • Furshpan, E. J. and Potter, D. D., 1968, Low-resistance junctions between cells in embryos and tissue culture. Current Topics in Development of Biology, 3, 95-127.
  • George, C. H., Kendall, J. M., Campbell, A. K. and Evans, W. H., 1998, Connexin-aequorin chimerae report cytoplasmic calcium environments along trafficking pathways leading to gap junction biogenesis in living COS-7 cells. Journal of Biological Chemistry, 273, 29822-29829.
  • George, C. H., Kendall, J. M. and Evans, W. H., 1999, lntracellular trafficking pathways in the assembly of connexins into gap junctions. Journal of Biological Chemistry, 274, 8678-8685.
  • Giaume, C. and Vena nee, L., 1998, Intercellular calcium signaling and gap junctional communication in astrocytes. Glia, 24, 50-64.
  • Giepmans, B. N. and Moolenaar, W. H., 1998, The gap junction protein connexin43 interacts with the second PDZ domain of the zona occludens-1 protein. Current Biology, 8, 931-934.
  • Giepmans, B. N., Verlann, I., Hengeveld, T., Janssen, H., Calafat, J., FaIk, M. M. and Moolenaar, W. H., 2001, Gap junction protein connexin-43 interacts directly with microtubules. Current Biology, 11, 1364-1368.
  • Goodenough, D. A., Goliger, J. A. and Paul, D. L., 1996, Connexins, connexons, and intercellular communication. Annual Reviews in Biochemistry, 65, 475-502.
  • Gutstein, D. E., Money, G. E., Vaidya, D., Liu, F., Chen, F. L, Stuhlmann, H. and Fishman, G. I., 2001, Heterogeneous expression of gap junction channels in the heart leads to conduction defects and ventricular dysfunction. Circulation. 104,1194-1199.
  • Haftek, M., Kowalewski, C., Mesnil, M., Blaszczyk, M. and Schnitt D., 1999, Intemalizaticn of gap junctions in benign familial pemphigus (Hailey-Hailey disease) and keratosis follicularis (Darter's disease). British Journal of Dermatology, 141, 224-230.
  • Hand, G. M., Muller, D. J., Nicholson, B. J., Engel, A. and Sosinsky, G. E., 2002, Isolation and characterisation of gap junctions from tissue culture cells. Journal of Molecular Biology. 315, 587-600.
  • Harris, A. L., 2001, Emerging issues of connexin channels: biophysics fills the gap. Quarterly Reviews in Biophysics, 34, 325-472.
  • He, D. S., Jiang, J. X., Taffet, S. M. and Burt, J. M., 1999, Formation of heteromeric gap junction channels by connexins 40 and 43 in vascular smooth muscle cells. Proceedings of the National Academy of Sciences (USA), 96, 6495-6500.
  • Hennemann, H., Dahl, E., White, J. B., Schwarz, H. J., Lalley, P. A., Chang, S., Nicholson, B. J. and Willecke, K., 1992, Two gap junction genes, connexin 31.1 and 30.3, are closely linked on mouse chromosome 4 and preferentially expressed in skin. Journal of Biological Chemistry, 267, 17225-17233.
  • Hulser, D. F., Eckert, R., lrmer, U., Krisciukaitis, A., Mindermann, A., Pleiss, J., Rehkof, B., Sharovskaya, J. and Traub, O., 1998, Intercellular communication via gap junctions. Journal of Bioelectrochemistry and Bioenergetics, 45, 55-65.
  • Hurtley, S. M. and Helenius, A., 1989, Protein oligomerization in the endoplasmic reticulum. Annual Reviews in Cell Biology, 5, 277307.
  • lsakson, B. E., Evans, W. H. and Boitano, S., 2001, Intercellular Ca2+ signaling in alveolar epithelial cells through gap junctions and by extracellular ATP. American Journal of Physiology, 280, L221-L228.
  • Jordan, K., Chodock, R., Hand, A. R. and Laird, D. W., 2001, The origin of annular junctions: a mechanism of gap junction intemalization. Journal of Cell Science, 114, 763-773.
  • Kelsell, D. P., Dunlop, J. and Hodgins, M. B., 2001, Human diseases: clues to cracking the connexin code? Trends in Cell Biology, 11, 2-6.
  • Kiehn, O. and Tresch, M. C., 2002, Gap junctions and motor behaviour. Trends in Neuroscience, 25, 108-115.
  • Kirchhoff, F., Dringen, R. and Giaume, C., 2001, Pathways of neuron-astrocyte interactions and their possible role in neuroprotection. European Archives in Psychiatry and Clinical Neurosdence, 251, 159-169.
  • Kirchhoff, S., Kim, J. S., Hagendorff, A., Thonnissen, E., Kruger, O., Lamers, W. H. and Willecke, K., 2000, Abnormal cardiac conduction and morphogenesis in connexin40 and connexin43 double-deficient mice. Circulation Research. 87, 346-348.
  • Klaunig, J. E. and Ruch, R. J., 1990, Role of inhibition of intercellular communication in carcinogenesis. Laboratory Investigation, 62, 135-145.
  • Klepeis, V. E., Cornell-Bell, A. and Trinkaus-Randall, V., 2001, Growth factors but not gap junctions play a role in injury-induced Ca2+ waves in epithelial cells. Journal of Cell Science, 114, 4185-4195.
  • Kojima, T., Fort, A., Tao, M., Yamamoto, M. and Spray, D., 2001, Gap junction expression and cell proliferation in differentiating cultures of Cx32 KO mouse hepatocytes. American Journal of Physiology, 281, G1004-G1013.
  • Kojima, T., Kokai, Y., Chiba, H., Yamamoto, M., Mochizuki, Y. and Sawada, N., 2001, Cx32 but not Cx26 is associated with tight junctions in primary cultures of rat hepatocytes. Experimental Cell Research, 263, 193-201.
  • Kojima, T., Sawada, N., Chiba, H., Kokai, Y., Yamamoto, M., Urban, M., Lee, G. H., Hertzberg, E. L, Mochizuki, Y. and Spray, D. C., 1999, Induction of tight junctions in human connexin 32 (hCx32)transfected mouse hepatocytes: connexin 32 interacts with occludin. Biochemical and Biophysical Research Communications, 266, 222-229.
  • Kojima, T., Sawada, N., Oyamada, M., Chiba, H., lsomura, H. and Mori, M., 1994, Rapid appearance of connexin 26-positive gap junctions in centrilobular hepatocytes without induction of mRNA and protein synthesis in isolated perfused liver of female rat. Journal of Cell Science, 107, 3579-3590.
  • Kondo, R. P., Wang, S.-Y., John, S. A., Weiss, J. N. and Goldhaber, J. I., 2000, Metabolic inhibition activates a non-selective current through connexin hemichannels in isolated ventricular myocytes. Journal of Molecular and Cellular Cardiology, 32, 1859-1872.
  • Krutovskikh, V. and Yamasaki, H., 2000, Connexin gene mutations in human genetic diseases. Mutation Research, 462, 197-207.
  • Krutovskikh, V. A., Troyanovsky, S. M., Piccoli, C., Tsuda, H., Asamoto, M. and Yamasaki, H., 2000, Differential effect of subcellular localization of communication impairing gap junction protein connexin43 on tumor cell growth in vivo. Oncogene, 19, 505-513.
  • Kumar, N. M. and GiIuIa, N. B., 1986, Cloning and characterisation of human and rat liver cDNAs encoding for a gap junction protein. Journal of Cell Biology. 103, 767-776.
  • Kumar, N. M. and GiIuIa, N. B., 1996, The gap junction communication channel. Cell, 84, 381-388.
  • Kumar, S. S., Varadaraj, K., Valiunas, V., Ramanan, S. V., Christensen, E. A., Beyer, E. C. and Brink, P. R., 2000, Functional expression and biophysical properties of polymorphic variants of the human gap junction protein connexin 37. Biochemical and Biophysical Research Communications, 274, 216-224.
  • Kwak, B. R., Hermans, H. R., DeJonge, H. R., Lohmann, S. M., Jongsma, H. J. and Chanson, M., 1995, Differential regulation of distinct types of gap junction channels by similar phosphorylating conditions. Molecular Biology of the Cell, 6, 1707-1719.
  • Laing, J. G. and Beyer, E. C., 2000, Degradation of gap junctions and connexins. Current Topics in Membranes, 49, 23-41.
  • Laird, D. W., Fistouris, P, Batist, G., Alpert, L., Huynh, H. T., Carystinos, G. D. and Alaoui-Jamali, M. A., 1999, Deficiency of connexin43 gap junctions is an independent marker for breast tumors. Cancer Research, 59, 4104-4110.
  • Lampe, P. D. and Lau, A. F., 2000, Regulation of gap junctions by phosphorylation of connexins. Archives of Biochemistry and Biophysics, 384, 205-215.
  • Lampe, P. D., Nguyen, B. P., Gil, S., Usui, M., Olerud, J., Takada, Y. and Carter, W. G., 1998, Cellular interaction of integrin alphasbe ta 1 with laminh 5 promotes gap functional communication. Journal of Cell Biology. 143, 1735-1747.
  • Lampe, P. D., Qui, Q., Meyer, R. A., TenBroek, E. M. Walseth, T. F., Starich, T. A., Grunenwald, H. L. and Johnson, R. G., 2001, Gap junction assembly: PTX-sensitive G proteins regulate the distribution of connexin43 within cells. American Journal of Physiology, 281, C1211-C1222.
  • Landesman, Y., White, T. W., Starich, T. A., Shaw, J. E., Goodenough, D. A. and Paul, D. L., 1999, lnnexin-3 forms connexin-like intercellular channels. Journal of Cell Science, 112, 2391-2396.
  • Lawrence, T. S., Beers, W. H. and GiIuIa, N. B., 1978, Transmission of hormonal stimulation by cell-to-cell communication. Nature, 272 501 - 506
  • Leite, M. F., Hirata, K., Pusl, T., Burgstahler, A. D., Okazaki, K., Ortega, J. M., Goes, A. M., Prado, M. A., Spray, D. C. and Nathanson, M. H., 2002, Molecular basis for pacemaker cells in epithelia. Journal of Biological Chemistry. 277,16313-16323.
  • Lemer, D. L., Yamada, K. A., Schuessler, R. B. and Saffitz, J. E., 2000, Accelerated onset and increased incidence of ventricular arrhythmias induced ischemia in Cx43-deficient mice. Circulation, 101, 547-552.
  • Leybert, L. and Sanderson, M. J., 2001, Intercellular calcium signaling and flash photolysis of caged compounds. A sensitive method to evaluate gap functional coupling. Methods in Molecular Biology, 154, 407-430.
  • Li, H., LIu, T. F., Lazarak, A., Peracchia, C., Goldberg, G. S., Lampe, P. D. and Johnson, R. G., 1996, Properties and regulation of gap functional hemichannels in the plasma membranes of cultured cells. Journal of Cell Biology, 134, 1019-1030.
  • Liao, Y., Day, K. H., Damon, D. N. and Duling, B. R., 2001, Endothelial cell-specific knockout of connexin 43 causes hypotension and bradycardia in mice. Proceedings of the National Academy of Sciences (USA), 98, 9989-9994.
  • Lin, J. H., Weigel, H., Cotrina, M. L, LIu, S., Bueno, E., Hansen, A. J., Hansen, T. W., Goldman, S. and Nedergaard, M., 1998, Gapjunction-mediated propagation and amplification of cell injury. Nature Neuroscience, 1, 494-500.
  • Lin, R., Wam-Cramer, B. J., Kurata, W. E. and Lau, A. F., 2001, vSrc phosphorylation of connexin 43 on Tyr247 and Tyr265 disrupts gap functional communication. Journal of Cell Biology, 154, 815-827.
  • Liu, X. Z., Xia, X. J., Adams, J., Chen, Z. Y., Welch, K. O., TeKin, M., Ouyang, X. M., Kristiansen, A., Pandya, A., Balkany, T., Arnos, K. S. and Nance, W. E., 2001, Mutations in GJA1 (connexin 43) are associated with non-syndromic autosomal recessive deafness. Human Molecular Genetics, 10, 2945-2951.
  • Lo, C. W., 2000, Role of gap junctions in cardiac conduction and development. Circulation Research, 87, 346-348.
  • Lo, C. W., Waldo, K. L. and Kirby, M. L., 1999, Gap junction communication and the modulation of cardiac neural crest cells. Trends in Cardiovascular Medicine, 9, 63-69.
  • Locke, D., Perusinghe, N., Newman, T., Jayatilake, H., Evans, W. H. and Monaghan, P., 2000, Development expression and assembly of connexins into homomeric and heteromeric gap junction hemichannels in the mouse mammary gland. Journal of Cell Physiology, 183, 228-237.
  • Loewenstein, W. R., 1979, Junctional intercellular communication and the control of growth. Biochimica et Biophysica Acte, 560,1 65.
  • Loewenstein, W. R., 1967, On the genesis of cellular communication. Developmental Biology, 6, 503-520.
  • Martin. P. E. M. and Evans, W. H., 2000, Trafficking and targeting to gap junctions of connexin 32 mutations to gap junction in CharcotMarie-Tooti X-linked disease. Current Topics in Membranes, 49, 461-481.
  • Martin. P. E. M., Blundell, G., Ahmad, S., Errington, R. J. and Evans, W. H., 2001, Multiple pathways in the trafficking and assembly of connexin 26, 32 and 43 into gap junction intercellular communication channels. Journal of Cell Science, 114, 3845-3855.
  • Martin. P. E. M., Coleman, S. L., Casalotti, S. O., Forge, A. and Evans, W. H., 1999, Properties of connexin26 gap Junctional proteins derived from mutations associated with non-syndromal hereditary deafness. Human Molecular Genetics, 8, 2369-2376.
  • Martin, P. E. M., Mambetisaeva, E. T., Archer, D. A., George, C. H. and Evans, W. H., 200Oa, Analysis of gap junction assembly using mutated connexins detected in Charcot-Marie-Tooti X-linked disease. Journal of Neurochemistry, 74, 711-720.
  • Martin. P. E. M., Steggles, J., Wilson, C., Ahmad, S. and Evans, W. H., 2000b, Targeting motifs and functional parameters governing the assembly of connexins into gap junctions. Biochemical Journal, 349, 281-287.
  • Malic, M., Evans, W. H., Brink, P. R. and Simon, M., 2002, Epidermal stem cells do not communicate through gap junctions. Journal of Investigative Dermatology. 118, 110-116.
  • Montecino-Rodriguez, E. and Dorshkind, K., 2001, Regulation of hematopoiesis by gap junction-mediated intercellular communication. Journal of Leukocyte Biology. 70, 341 -347.
  • Moriey, G. E., Taffet, S. M. and Delmar, M., 1996, The carboxylterminal of connexin43 alters pH regulation of connexin 32 channels. Biophysical Journal. 70, 1294-1302.
  • Musil, L. S. and Goodenough, D. A., 1993, Multisubunit assembly of an integral plasma membrane channel protein, gap junction connexin43, occurs after exit from the ER. Cell. 74, 1065-1077.
  • Musil, L. S., VanSlyke, J. K. and Roberts, L. M., 2000, Regulation of connexin degradation as a mechanism to increase gap junction assembly and function. Journal of Biological Chemistry, 18, 25207-25215.
  • Nadarajah, B., Jones, A. M., Evans, W. H. and Pamavelas, J. G., 1997, Differential expression of connexins during neocortical development and neuronal circuit formation. Journal of Neurosdence, 17,3096-3111.
  • Nagy, J. I., Patel, D., Ochalski, P. A. and Stelmack, G. L., 1999, Connexin30 in rodent cat and human brain: selective expression in grey matter astrocytes, co-localization with connexin43 at gap junctions and late developmental appearance. Neuroscience, 88, 447-468.
  • Nells, E., Haites, N. and Broeckhoven, C., 1999, Mutations in peripheral myelin genes and associated genes in inherited peripheral neuropathies. Human mutations, 13, 11-28.
  • Nelles, E., Budzler, C., Jung, D., Temme, A., Gabriel, H. D., Dahl, U., Traub, O., Stumpel, F., Jungermann, K., Zielasek, J., Toyka, K. V., Dermietzel, R. and Willecke, K., 1996, Defective propagation of signals generated by sympathetic nerve stimulation in the liver of connexin32-deficient mice. Proceedings of the National Academy of Sciences (USA). 93, 9565-9570.
  • Nicholson, B. J., Weber, P. A., Cao, F., Chang, H. C., Lampe, P. and Goldberg, G., 2002, The molecular basis of selective permeability of connexins is complex and includes both size and charge. Brazilian Journal of Medicine and Biology Research, 33, 369398.
  • Niessen, H., Harz, H., Bedner, P., Kramer, K. and Willecke, K., 2000, Selective permeability of different connexin channels to the second messenger inositol 1, 4, 5-trisphosphate. Journal of Cell Science. 113, 1365-1372.
  • Nishii, K., Kumai, M. and Shibata, Y., 2001, Regulation of the epithelial-mesenchymal transformation through gap junction channels in heart development Trends in Cardiovascular Medicine, 11, 213-218.
  • Nusrat, A., Chen, J. A., Foley, C. S., Liang, T. W., Tom, J., Cromwell, M., Quan, C. and Mrsny, R., 2000, The coiled coil domain of ocdudin can act to organise structural and functional elements of the epithelial tight junction. Journal of Biological Chemistry, 275, 29816-29822.
  • Oh, S., Ri, Y., Bennett, M. V. L., Trexier, E. B., Verselis, V. K. and Bargiello, T. A., 1997, Changes in permeability caused by connexin 32 mutations underlie X-linked Charcot-Marie-Tooth disease. Neuron, 19, 927-938.
  • Omori, Y., Mesnil, M. and Yamasaki, H., 1996, Connexin 32 mutations from X-linked Charcot-Marie-Tooth disease patients: functional defects and dominant negative effects. Molecular Biology of the Cell, 7, 907-916.
  • Omori, Y., Zaiden-Dagli, M. L., Yamakage, K. and Yamasaki, H., 2001, Involvement of gap junctions in tumor suppression: analysis of genetically-manipulated mice. Mutation Research, 477, 191 196.
  • Oviedo-Orta, E., Gasque, P. and Evans, W. H., 2001, lmmunoglobulin and cytokine expression in mixed lymphocyte cultures in reduced by disruption of gap junction intercellular communication. FASEB Journal. 15, 768-774.
  • Oviedo-Orta, E., Hoy, T. and Evans, W. H., 2000, Intercellular communication in the immune system: differentia expression of connexin40 and 43, and perturbation of gap junction channel functions in peripheral blood and tonsil human lymphocyte subpopulations. Immunology, 99, 578-590.
  • Paemeleire, K., Martin. P. E. M., Coleman, S. L., Fogarty, K. E., Carrington, W. A., Leybaert, L., Tuft, R. A., Evans, W. H. and Sanderson, M. J., 2000, Intercellular calcium waves in HeLa cells expressing GFP-labeled connexin 43,32, or 26. Molecular Biology of the CeII, 11, 1815-1827.
  • Pal, J. D., Liu, X., Mackay, D., ShIeIs, A, Berthoud, V. M., Beyer, E. C. and Ebihara, L., 2000, Connexin46 mutations linked to congenital cataract show loss of gap junction channel function. American Journal of Physiology, 279, C596-C602.
  • Paul, D. L., 1986, Molecular doning of cDNA for rat liver gap junction protein. Journal of Cell Biology. 103, 123-134.
  • Paulson, A. F., Lampe, P. D., Meyer, R. A., TenBroek, E. M., Atkinson, M. M., Walseth, T. F. and Johnson, R. G., 2000, Cyclic AMP and LDL trigger a rapid enhancement in gap junction assembly through a stimulation of connexin trafficking. Journal of Cell Science, 113, 3037-3049.
  • Peinado, A., Yuste, R. and Katz, L. C., 1993, Extensive dye coupling between rat neocordcal neurons during the period of circuit formation. Neuron, 10, 103-114.
  • Peracchia, C., Sotkis, A., Wang, X. G., Peracchia, L. L. and Persechini, A., 2000, Calmodulin directly gates gap junction channels. Journal of Biological Chemistry, 275, 26220-26224.
  • Perkins, G., Goodenough, D. and Sosinky, G., 1997, Threedimensional structure of the gap junction connexon. Biophysical Journal, 72, 533-544.
  • Phelan, P. and Starich, T. A., 2001, Innexins get into the gap. Bioessays, 23, 388-396.
  • Pitts. J. D., 1998, The discovery of metabolic co-operation. Bioessays, 20, 1047-1051.
  • Plum, A., Winterhager, E., Pesch, J., Lautermann, J, Hailas, G., Rosentreter, B., Traub, O., Herberhold, C. and Willecke, K., 2001, Connexin31-deficiency in mice causes transient placental dysmorhogenesis but does not impair hearing and skin differentiation. Developments in Biology, 231, 334-347.
  • Pol, A., Ortega, D. and Enrich, C., 1997, Identification and distribution of proteins in isolated endosomal fractions of rat liver: involvement in endocytosis, recycling and transcytosis. BiochemicalJournal, 323, 435-443.
  • Purdue, P. E. and Lazarow, P. B., 2001, Peroxisome biogenesis. Annual Reviews in Cell Development Biology, 17, 701-752.
  • Pumick, P. E., Benjamin, D. C., Verselis, V. K., Bargiello, T. A. and Dowd, T. L., 2000, Structure of the amino terminus of a gap junction protein. Archives of Biochemistry and Biophysics, 381, 181-190.
  • Purves, D., Augustine, G. J., Fitzpatrick, D., Katz, L. C., LaMantia, J. O., McNamara, J. O. and Williams, S. M., 2001, in Neurosdence (Sunderiand, MA: Sinauer Assoc), p. 681.
  • Qu, Y. and Dahl, G., 2002. Function of the voltage gate of gap junction channels: selective exclusion of molecules. Proceedings of the National Academy of Sciences (USA), 99, 697-702.
  • Rabionet, R., Gasparini, P. and Estivill, X., 2000, Molecular genetics of hearing impairment due to mutations in gap junction genes encoding beta connexins. Human Mutations, 16, 190-202.
  • Rash, J. E., Yasumura, T. and Dudek, F. E., 1998, Ultrastructure, histological distribution, and freeze-fracture immunocytochemistry of gap junctions in rat brain and spinal cord. Cell Biology International, 22, 731-749.
  • Reaume, A. G., de Sousa, P. A., Kulkami, S., Languie, B. L., Zhu, D., Davies, T. C., Juneja, S. C., Kidder, G. M. and Rossant, J., 1995, Cardiac malformation in neonatal mice lacking connexin 43, Science. 2ST. 1831-1834.
  • Revel, J. P. and Kamovsky, M. J., 1967, Hexagonal array of subunits in intercellular junctions of the mouse heart and liver. Journal of Cell Biology, 33, C7-C12.
  • Revilla, A., Bennett, M. V. L. and Barrio, L. C., 2000, Molecular determinante of membrane potential dependence in vertebrate gap junction channels. Proceedings of the National Academy of Sciences (USA), 97, 14760-14765.
  • Robertson, J. D., 1963, The occurrence of a subunit pattern in the unit membrane of club endings in Mauthner cell synapses in gold fish brains. Journal of Cell Biology, 19, 201-221.
  • Romanello, M. and D'Andreau, P., 2001, Dual mechanism of intercellular communication in HOBIT osteoblastic cells: a role for gap-junctional hemichannels. Journal of Bone and Mineral Research, 16, 1465-1476.
  • Rose, B. and Loewenstein, W. R., 1976, Permeability of a cell junction and the local cytoplasmic free ionised calcium concentration; a study with aequorin. Journal of Membrane Biology, 28, 87119.
  • Rosendaal, M. and Krenacs, T. T., 2000, Regulatory pathways in blood-forming tissue with particular reference to gap junctional communication. Pathology and Oncology Research, 6, 243-249.
  • Rouan, F., White, T. W., Brown, N., Taylor, A. M., Lucke, T. W., Paul, D. L., Munro, C. S., Uitto, J., Hodgins, M. B. and Richard, G., 2001, Trans-dominant inhibition of connexin-43 by mutant connexin-26: implications for dominant connexin disorders affecting epidermal differentiation Journal of Cell Science, 114, 21052113.
  • Rufer, M., Wirth, S. B., Hofer, A., Dermietzel, R., Pastor, A., Kettenmann, H. and Unsicker, K., 1996, Regulation of connexin43, GFAP, and FGF-2 is not accompanied by changes in astroglial coupling in MPTP-lesioned, FGF-2 treated parkinsonian mice. Journal of Neuroscience Research, 46, 606-617.
  • Rutz, M. L. and Hulser, D. F., 2001, Supramolecular dynamics of gap junctions. European Journal of Cell Biology, 80, 20-30.
  • Saez, J. C., Connor, J. A., Spray, D. C. and Bennett, M. V., 1989, Hepatocyte gap junctions are permeable to the second messenger, inositol 1,4,5-trisphosphate, and to calcium ions. Proceedings of the National Academy of Sciences (USA), 86, 2708-2712.
  • Saffltz, J. E., 2000, Regulation of intercellular coupling in acute and chronic heart disease. Brazilian Journal of Mediane and Biology Research. 33, 407-413.
  • Sanderson, M. J., Charles, A. C., Boitano, S. and Dirksen, E. R., 1994, Mechanisms and function of intercellular calcium signaling. Molecular Cell Endocrinology, 98, 173-187.
  • Sandow, S. L. and Hill, C. E., 2000, Incidence of myoendothelial gap junctions in the proximal and distal mesentere arteries of the rat is suggestive of a role in the endothelium derived hyperpolarising factor-mediated response. Circulation Research, 86, 341-346.
  • Sarma, T. D., Meyer, R. A., Wang, F., Abraham, V., Lo, C. W. and Koval, M., 2001, Multimeric connexin interactions prior to the trans-Golgi network. Journal of Cell Science, 114,4013-4024.
  • Schubert, A., Schubert, W., Spray, D. C. and Lisantl, M. P., 2002, Connexin family members target to NpkJ raft domains/caveolae and interact with caveolin-1. Biochemistry. 41,5754-5764.
  • Severs, N. J., Rothery, S., Dupont, E., Coppen, S. R., Yeh, H.-l., Ko, Y.-S., Matsushita, T., Kaba, R. and Halliday, D., 2001, lmmuno cytochemical analysis of connexin expression in the healthy and diseased cardiovascular system. Microscopy Research and Technology. 52, 301-322.
  • Simon, A. M., Goodenough, D. A. and Paul, D. L., 1998, Mice lacking connexin40 have cardiac conduction abnormalites characteristic of atrioventricular block and bundle branch block. Current Biology, 8, 295-298.
  • Spray, D. C. and Dermietzel, R., 1996, Neuroscience Intelligence Unit: Gap junctions in the nervous system (New York: Springer).
  • Stebbings, L. A., Todman, M. G., Phelan, P., Bacon, J. P. and Davies, J. A., 2000, Two Drosophila innexins are expressed in overlapping domains and co-operate to form gap-junction channels. Molecular Biology of the Cell, 11, 2459-2470.
  • Steel, K. P. and Kras, C. J., 2001, A genetic approach to understanding auditory function. Nature Genetics, 27, 143-149.
  • Stout C. E., Constatntin, J. L., Naus, C. C. and Charles, A. C., 2002, Intercellular calcium signaling in astrocytes via ATP release through connexin hemichannels. Journal of Biological Chemistry, 277, 10482-10488.
  • Suchyna, T. M., Nitsche, J. M., Chilton, M., Harris, A. L., Veenstra, R. D. and Nicholson, B. J., 1999, Different ionic selectivities for connexins 26 and 32 produce rectifying gap junction channels. Biophysical Journal, 77, 2968-2987.
  • Temme, A., Ott, T., Haberberger, T., Traub, O. and Willecke, K., 2000, Acute-phase response and circadian expression of connexin26 are not altered in connexin32-deficient mouse liver. Cell and Tissue Research, 300, 111-117.
  • TenBroek, E. M., Lampe, P. D., Solan, J. L., Reynhout, J. K. and Johnson, R. G., 2001, Ser364 of connexin43 and the upregulation of gap junction assembly by cAMP. Journal of Cell Biology, 155, 1307-1318.
  • Torok, K., Stauffer, K. and Evans, W. H., 1997, Connexin 32 of gap junctions contains two cytoplasmic calmoldulin-binding domains. Biochemical Journal, 326, 479-483.
  • Toyofuku, T., Yabuki, M., Otsu, K., Kuzuya, T., Hori, M. and Tada, M., 1998, Direct association of the gap junction protein connexin43 with ZO-1 in cardiac myocytes. Journal of Biological Chemistry, 172, 12725-12731.
  • Trosko, J. E. and Ruch, R. J., Cell-eil communication in carcinogenesis. Frontlets in Biosdence, 3, D208-D236.
  • Unger, V. M., Kumar, N. M., GiIuIa, N. B. and Yeager, M., 1999, Three-dimensional structure of a recombinant gap junction membrane channel. Science, 283, 1176-1180.
  • Unwin, N., 1989, The structure of ion channels in membranes of excitable cells. Neuron, 3, 665-676.
  • Valiunas, V. and Weingart, R., 2000, Electrical properties of gap junction hemichanneb identified in transfected HeLa cells. Pflugers Archives, 440, 366-379.
  • Valiunas, V., Weingart, R. and Brink, P. R., 2000, Formation of heterotypb gap junction channels by connexins 40 and 43. Circulation Research, 86, E42-E49.
  • VanSlyke, J. K., Deschenes, S. M. and Musil, L. S., 2000, lntracellular transport, assembly, and degradation of wild-type and disease-linked mutant gap junction proteins. Molecular Biology of the Cell, 11, 1933-1946.
  • Vis, J. C., Nicholson, L. F., Faull, R. L., Evans, W. H., Severs, N. J. and Green, C. R., 1998, Connexin expression in Huntington's diseased human brain. Cell Biology International, 22, 837-847.
  • Warner, A., dements, D. K., Parikh, S., Evans, W. H. and DeHaan, R. L., 1995, Specific motifs in the external loops of connexin proteins can determine gap junction formation between chick heart myocytes. Journal of Physiology, 488, 721-728.
  • Watanabe, A., 1958, The interaction of electrical activity among neurons of lobster cardiac ganglion. Japanese Journal of Physiology. 8, 305-318.
  • Weidmann, S., 1969, Electrical coupling between myocardial cells. Progress in Brain Research, 31, 275-281.
  • White, T. W., 2002, Unique and redundant connexin contribution to lens development Science, 295, 319-320.
  • White, T. W. and Paul, D. L., 1999, Genetic diseases and gene knockouts reveal diverse connexin functions. Annual Reviews in Physiology, 61,283-310.
  • White, T. W., Paul, D. L., Goodenough, D. A. and Bruzzone, R., 1995, Functional analysis of selective interactions among rodent connexins. Molecular Biology of the Cell. 6, 459-470.
  • WiIlecke, K., Eiberger, J., Degen, J., Eckardt, D., Romualdi, A., Guldenagel, M., Deutsch, U. and Soehl, G., 2002, Structural and functional diversity of connexin genes in the mouse and human genome. Biological Chemistry, 383, 725-737.
  • Yeager, M. and Nicholson, B. J., 1996, Structure of gap junction intercellular channels. Current Opinions in Structural Biology, 6, 183-192.
  • Zhang, J.-T. L, Chen, M., Foote, C. I. and Nicholson, B. J., 1996, Membrane integration of in vitro-translated gap functional proteins: Co- and post-translational mechanisms. Molecular Biology of the Ce//, 7, 471-482.
  • Zhou, X. W., Pfahnl, A., Werner, R., Hudder, A., Lianes, A., Lubeke, A. and Dahl, G., 1997, Identification of a pore lining segment in gap junction hemichannels. Biophysical Journal, 72, 1946-1953.

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