Publication Cover
Journal of Environmental Science and Health, Part A
Toxic/Hazardous Substances and Environmental Engineering
Volume 53, 2018 - Issue 2
355
Views
5
CrossRef citations to date
0
Altmetric
Original Articles

Oxidative stress response in SH-SY5Y cells exposed to short-term 1800 MHz radiofrequency radiation

, &
Pages 132-138 | Received 21 Jun 2017, Accepted 06 Sep 2017, Published online: 17 Nov 2017

References

  • ICNIRP, International Commission on Non-Ionizing Radiation Protection, Guidelines for Limiting Exposure to Time-Varying Electric, Magnetic, and Electromagnetic Fields (up to 300 GHz). Health Phys. 1998, 74, 494–522.
  • IARC, International Agency for Research on Cancer Working Group on the Evaluation of Carcinogenic Risks to Humans. Non-ionizing Radiation, Part II: Radiofrequency Electromagnetic Fields, IARC: Lyon, 2011.
  • Marjanović, A. M.; Pavičić, I.; Trošić, I. Biological Indicators in Response to Radiofrequency/Microwave Exposure. Arh. Hig. Rada. Toksikol. 2012, 63, 407–416.
  • Yakymenko, I.; Tsybulin, O.; Sidorik, E.; Henshel, D.; Kyrylenko, O.; Kyrylenko, S. Oxidative Mechanisms of Biological Activity of Low-Intensity Radiofrequency Radiation. Electromagn. Biol. Med. 2016, 35(2)), 186–202.
  • Nazıroğlu, M.; Ciğ, B.; Doğan, S.; Uğuz, A.C.; Dilek, S.; Faouzi, D. 2.45-Gz Wireless Devices Induce Oxidative Stress and Proliferation through Cytosolic Ca2+ Influx in Human Leukemia Cancer Cells. Int. J. Radiat. Biol. 2012, 88, 449–456.
  • Ni, S.; Yu, Y.; Zhang, Y.; Wu, W.; Lai, K.; Yao, K. Study of Oxidative Stress in Human Lens Epithelial Cells Exposed to 1.8 GHz Radiofrequency Fields. PLoS One. 2013, 8 e72370.
  • Sefidbakht, Y.; Moosavi-Movahedi, A. A.; Hosseinkhani, S.; Khodagholi, F.; Torkzadeh-Mahani, M.; Fooland, F.; Faraji-Dana, R. Effects of 940 MHz EMF on Bioluminescence and Oxidative Response of Stable Luciferase Producing HEK Cells. Photochem. Photobiol. Sci. 2014, 13, 1082–1092.
  • De Iuliis, G. N.; Newey, R. J.; King, B. V.; Aitken, R. J. Mobile Phone Radiation Induces Reactive Oxygen Species Production and DNA Damage in Human Spermatozoa in Vitro. PLoS One 2009, 4, e6446.
  • Balci, M.; Devrim, E.; Durak, I. Effects of Mobile Phones on Oxidant/Antioxidant Balance in Cornea and Lens of Rats. Curr. Eye Res. 2007, 32, 21–25.
  • Esmekaya, M. A.; Ozer, C.; Seyhan, N. 900 MHz Pulse-Modulated Radiofrequency Radiation Induces Oxidative Stress on Heart, Lung, Testis and Liver Tissues. Gen. Physiol. Biophys. 2011, 30, 84–89.
  • Yurekli, A. I.; Ozkan, M.; Kalkan, T.; Saybasili, H.; Tuncel, H.; Atukeren, P.; Gumustas, K.; Seker, S. GSM Base Station Electromagnetic Radiation and Oxidative Stress in Rats. Electromagn. Biol. Med. 2006, 25, 177–188.
  • Tkalec, M.; Štambuk, A.; Šrut, M.; Malarić, K.; Klobučar, G. I. Oxidative and Genotoxic Effects of 900MHz Electromagnetic Fields in the Earthworm Eisenia Fetida. Ecotoxicol. Environ. Saf. 2013, 90, 7–12.
  • Crouzier, D.; Perrin, A.; Torres, G.; Dabouis, V.; Debouzy, J. C. Pulsed Electromagnetic Field at 9.71 GHz Increase Free Radical Production in Yeast (Saccharomyces cerevisiae) Pathol. Biol. 2009, 57, 245–251.
  • Tkalec, M.; Malarić, K.; Pevalek-Kozlina, B. Exposure to Radiofrequency Radiation Induces Oxidative Stress in Duckweed Lemna Minor L. Sci. Total Environ. 2007, 388, 78–89.
  • Demirel, S.; Doganay, S.; Turkoz, Y.; Dogan, Z.; Turan, B.; Firat, P.G. Effects of Third Generation Mobile Phone-Emitted Electromagnetic Radiation on Oxidative Stress Parameters in Eye Tissue and Blood of Rats. Cutan. Ocul. Toxicol. 2012, 31, 89–94.
  • Hong, M. N.; Kim, B. C.; Ko, Y. G.; Lee, Y. S.; Hong, S. C.; Kim, T.; Pack, J. K.; Choi, H. D.; Kim, N.; Lee, J. S. Effects of 837 and 1950 MHz Radiofrequency Radiation Exposure Alone or Combined on Oxidative Stress in MCF10A Cells. Bioelectromagnetics 2012, 33, 604–611.
  • Huang, T. Q.; Lee, M. S.; Oh, E.; Zhang, B. T.; Seo, J. S.; Park, W. Y. Molecular Responses of Jurkat T-cells to 1763 MHz Radiofrequency Radiation. Int. J. Radiat. Biol. 2008, 84, 734–741.
  • Lantow, M.; Lupke, M.; Frahm, J.; Mattsson, M.O.; Kuster, N.; Simko, M. ROS Release and Hsp70 Expression After Exposure to 1,800 MHz Radiofrequency Electromagnetic Fields in Primary Human Monocytes and Lymphocytes. Radiat. Environ. Biophys. 2006, 45, 55–62.
  • Xu, S.; Ning, W.; Xu, Z.; Zhou, S.; Chiang, H.; Luo, J. Chronic Exposure to GSM 1800-MHz Microwaves Reduces Excitatory Synaptic Activity in Cultured Hippocampal Neurons. Neurosci. Lett. 2006, 398, 253–257.
  • Chen, C.; Ma, Q.; Liu, C.; Deng, P.; Zhu, G.; Zhang, L.; He, M.; Lu, Y.; Duan W.; Pei, L.; Li, M.; Yu, Z.; Zhou, Z. Exposure to 1800 MHz Radiofrequency Radiation Impairs Neurite Outgrowth of Embryonic Neural Stem Cells. Sci. Rep. 2014, 4, 5103.
  • Motawi, T. K.; Darwish, H. A.; Moustafa, Y. M.; Labib, M. M. Biochemical Modifications and Neuronal Damage in Brain of Young and Adult Rats After Long-Term Exposure to Mobile Phone Radiations. Cell. Biochem. Biophys. 2014, 70, 845–855.
  • Saikhedkar, N.; Bhatnagar, M.; Jain, A.; Sukhwal, P.; Sharma, C.; Jaiswal, N. Effects of Mobile Phone Radiation (900 MHz Radiofrequency) on Structure and Functions of Rat Brain. Neurol. Res. 2014, 36, 1072–1079.
  • Şahin, A.; Aslan, A.; Baş, O.; İkinci, A.; Özyılmaz, C.; Sönmez, O. F.; Çolakoğlu, S.; Odacı, E. Deleterious Impacts of a 900-MHz Electromagnetic Field on Hippocampal Pyramidal Neurons of 8-Week-Old Sprague–Dawley Male Rats. Brain Res. 2015, 1624, 232–238.
  • Xie, H.; Hu, L.; Li, G. SH-SY5Y Human Neuroblastoma Cell Line: In Vitro Cell Model of Dopaminergic Neurons in Parkinson's Disease. Chin. Med. J. 2010, 123, 1086–1092.
  • Freshney, R. I. Cell Lines, Protocol 12.2. In: Culture of Animal Cells: A Manual of Basic Techniques, 4th ed, Freshney, R. I., Ed, Viley-Liss Inc.: New York, 2000, 184–188.
  • Steffensen, K.V.; Raskmark, P.; Pedersen, G.F. FDTD Calculations of the EM-Field Distribution in a Microtiter Suspension Well. In: Proceedings of the COST 244. Biomedical 26. Effects of Electromagnetic Fields, Finland, 1995, 80–87.
  • McIntosh, R. L.; McKenzie, R. J.; Iskra, S.; Carratell, A.; Standaerz, P. Computation of SAR in Cell Culture Fasks Exposed to 900 MHz GSM Type Signals in a Modified TEM Cell. Appl. Comput. Electromagn Soc. J. 2003, 18, 107–113.
  • Bradford, M. A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding. Anal. Biochem. 1976, 72, 248–254.
  • Abel, S.; Gelderblom, W. Oxidative Damage and Fumonisin B1-Induced Toxicity in Primary Rat Hepatocytes and Rat Liver in Vivo. Toxicology 1998, 131, 121–131.
  • Tietze, F. Enzymic Method for Quantitative Determination of Nanogram Amounts of Total and Oxidized Glutathione: Applications to Mammalian Blood and Other Tissues. Anal Biochem. 1969, 27, 502–522.
  • Dalle-Donne, I.; Rossi, R.; Colombo, R.; Giustarini, D.; Milzani, A. Biomarkers of Oxidative Damage in Human Disease. Clin Chem. 2006, 52, 601–623.
  • Lukac, N.; Massanyi, P.; Roychoudhury, S.; Capcarova, M.; Tvrda, E.; Knazicka, Z.; Kolesarova, A.; Danko, J. In Vitro Effects of Radiofrequency Electromagnetic Waves on Bovine Spermatozoa Motility. J. Environ. Sci. Health A: Tox. Hazard Subst. Environ. Eng. 2011, 46 (12), 1417–1423.
  • Valbonesi, P.; Franzellitti, S.; Bersani, F.; Contin, A.; Fabbri, E. Effects of the Exposure to Intermittent 1.8 GHz Radio Frequency Electromagnetic Fields on HSP70 Expression and MAPK Signaling Pathways in PC12 Cells. Int. J. Radiat. Biol. 2014, 90, 382–391.
  • Xu, S.; Chen, G.; Chen, C.; Sun, C.; Zhang, D.; Murbach, M.; Kuster, N.; Zeng, Q.; Xu, Z. Cell Type-Dependent Induction of DNA Damage by 1800 MHz Radiofrequency Electromagnetic Fields does not Result in Significant Cellular Dysfunctions. PLoS One 2013, 8, e54906.
  • Yao, K.; Wu, W.; Wang, K.; Ni, S.; Ye, P.; Yu, Y.; Ye, J.; Sun, L. Electromagnetic Noise Inhibits Radiofrequency Radiation-Induced DNA Damage and Reactive Oxygen Species Increase in Human Lens Epithelial Cells. Mol. Vis. 2008, 14, 964–969.
  • Zhao, T. Y.; Zou, S. P.; Knapp, P. E. Exposure to Cell Phone Radiation Up-Regulates Apoptosis Genes in Primary Cultures of Neurons and Astrocytes. Neurosci. Lett. 2007, 412, 34–38.
  • Nylund, R.; Leszczynski, D. Mobile Phone Radiation Causes Changes in Gene and Protein Expression in Human Endothelial Cell Lines and the Response Seems to be Genome and Proteome-Dependent. Proteomics 2006, 6, 4769–4780.
  • Leszczynski, D.; Joenväärä, S.; Reivinen, J.; Kuokka, R. Non-Thermal Activation of the hsp27/p38MAPK Stress Pathway by Mobile Phone Radiation in Human Endothelial Cells: Molecular Mechanism for Cancer- and Blood–Brain Barrier-Related Effects. Differentiation 2002, 70, 120–129.
  • Leszczynski, D.; Nylund, R.; Joenvaara, S.; Reivinen, J. Applicability of Discovery Science Approach to Determine Biological Effects of Mobile Phone Radiation. Proteomics 2004, 4, 426–431.
  • Nylund, R.; Leszczynski, D. Proteomics Analysis of Human Endothelial Cell Line EA.hy926 After Exposure to GSM 900 Radiation. Proteomics 2004, 4, 1359–1365.
  • Ballardin, M.; Tusa, I.; Fontana, N.; Monorchio, A.; Pelletti, C.; Rogovich, A.; Barale, R.; Scarpato, R. Non-Thermal Effects of 2.45 GHz Microwaves on Spindle Assembly, Mitotic Cells and Viability of Chinese Hamster V-79 Cells. Mutat. Res. 2011, 716, 1–9.
  • Pavicic, I.; Trosic, I. In Vitro Testing of Cellular Response to Ultra High Frequency Electromagnetic Field Radiation. Toxicol. In Vitro 2008a, 22, 1344–1348.
  • Höytö, A.; Luukkonen, J.; Juutilainen, J.; Naarala, J. Proliferation, Oxidative Stress and Cell Death in Cells Exposed to 872 MHz Radiofrequency Radiation and Oxidants. Radiat. Res. 2008, 170, 235–243.
  • Pavicic, I.; Trosic, I. Impact of 864 MHz or 935 MHz Radiofrequency Microwave Radiation on the Basic Growth Parameters of V79 Cell Line. Acta Biol. Hung. 2008b, 59, 67–76.
  • Stander, B.A.; Marais, S.; Huyser, C.; Fourie, Z.; Leszczynski, D.; Jouber, A. M. Effects of Non-Thermal Mobile Phone Radiation on Breast Adenocarcinoma Cells. S. Afr. J. Sci. 2011, 107, 1–9.
  • Del Vecchio, G.; Giuliani, A.; Fernandez, M.; Mesirca, P.; Bersani, F.; Pinto, R.; Ardoino, L.; Lovisolo, G. A.; Giardino, L.; Calzà, L. Effect of Radiofrequency Electromagnetic Field Exposure on in Vitro Models of Neurodegenerative Disease. Bioelectromagnetics 2009, 30, 564–572.
  • Xu, S.; Zhou, Z.; Zhang, L.; Yu, Z.; Zhang, W.; Wang, Y.; Wang, X.; Li, M.; Chen, C.; He, M.; Zhang, G.; Zhong, M. Exposure to 1800 MHz Radiofrequency Radiation Induces Oxidative Damage to Mitochondrial DNA in Primary Cultured Neurons. Brain Res. 2010, 1311, 189–196.
  • Campisi, A.; Gulino, M.; Acquaviva, R.; Bellia, P.; Raciti, G.; Grasso, R.; Musumeci, F.; Vanella, A.; Triglia, A. Reactive Oxygen Species Levels and DNA Fragmentation on Astrocytes in Primary Culture After Acute Exposure to Low Intensity Microwave Electromagnetic Field. Neurosci. Lett. 2010, 473, 52–55.
  • Aydin, B.; Akar, A. Effects of a 900-MHz Electromagnetic Field on Oxidative Stress Parameters in Rat Lymphoid Organs, Polymorphonuclear Leukocytes and Plasma. Arch. Med. Res. 2011, 42, 261–267.
  • Dasdag, S.; Bilgin, H. M.; Akdag, M. Z.; Celik, H.; Aksen, F. Effects of Long Term Mobile Phone Exposure on Oxidative–Antioxidative Processes and Nitric Oxide in Rats. Biotechnol. Biotechnol. Equip. 2008, 22, 992–927.
  • Marjanovic, A. M.; Pavicic, I.; Trosic, I. Cell Oxidation-Reduction Imbalance After Modulated Radiofrequency Radiation. Electromagn. Biol. Med. 2015, 34(4), 381–386.
  • Ozguner, F.; Altinbas, A.; Ozaydin, M.; Dogan, A.; Vural, H.; Kisioglu, A. N.; Cesur, G.; Yildirim, N. G. Mobile Phone-Induced Myocardial Oxidative Stress: Protection by a Novel Antioxidant Agent Caffeic Acid Phenethyl Ester. Toxicol. Ind. Health 2005, 21, 223–230.
  • Jelodar, G.; Akbari, A.; Nazifi, S. The Prophylactic Effect of Vitamin C on Induced Oxidative Stress in Rat Testis Following Exposure to 900 MHz Radio Frequency Wave Generated by a BTS Antenna Model. Electromagn. Biol. Med. 2013, 32, 409–416.
  • Ilhan, A.; Gurel, A.; Armutcu, F.; Kamisli, S.; Iraz, M.; Akyol, O.; Ozen, S. Ginkgo Biloba Prevents Mobile Phone-Induced Oxidative Stress in Rat Brain. Clin. Chim. Acta. 2004, 340, 153–162.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.