590
Views
9
CrossRef citations to date
0
Altmetric
Report

A rapid double-staining technique to improve seed viability testing in terrestrial orchids

ORCID Icon, & ORCID Icon
Pages 877-882 | Received 20 Sep 2018, Accepted 11 Feb 2019, Published online: 29 Mar 2019

References

  • Barsberg ST, Lee Y-I, Rasmussen HN. 2018. Development of C-lignin with G/S-lignin and lipids in orchid seed coats – an unexpected diversity exposed by ATR-FT-IR spectroscopy. Seed Sci Res. 28(01):41–51.[CrossRef][10.1017/S0960258517000344][InsertedFromOnline]
  • Batty AL, Dixon KW, Brundrett M, Sivasithamparam K. 2001. Long-term storage of mycorrhizal fungi and seed as a tool for the conservation of endangered Western Australian terrestrial orchids. Aust J Bot. 49(5):619–628.
  • Colville L, Marks T, Pritchard H, Custódio C, Machado-Neto N. 2016. Development of a reliable GC-MS method for fatty acid profiling using direct transesterification of minimal quantities of microscopic orchid seeds. Seed Sci Res. 26(1):84–91.
  • Custódio CC, Marks TR, Pritchard HW, Hosomi ST, Machado-Neto NB. 2016. Improved tetrazolium viability testing in orchid seeds with a thick carapace (Dactylorhiza fuchsii) or dark seed coat (Vanda curvifolia. Seed Sci Technol. 44(1):1–12.
  • Deswal DP, Chand U. 1997. Standardization of the tetrazolium test for viability estimation in ricebean (Vigna umbellata (Thunb.) Ohwi & Ohashi) Seeds. Seed Sci Technol. 25:409–417.
  • Dowling N, Jusaitis M. 2012. Asymbiotic in vitro germination and seed quality assessment of Australian terrestrial orchids. Aust J Bot. 60(7):592–601.
  • Dressler RL. 1993. Phylogeny and classification of the orchid family. Oregon: Dioscorides Press.
  • Fast G. 1982. European terrestrial orchids. Symbiotic and asymbiotic methods. In: Arditti J, editor. Orchid biology – reviews and perspectives II. Ithaca (NY): Comstock Publishing Associates; pp. 326–329.
  • Frosh W. 1982. Beseitigung der durch die innere Hülle bedingten Keimhammung bei europäischen Orchideen. Die Orchidee. 33:145–146.
  • Harvais G. 1980. Scientific notes on a Cypripedium reginae of northwestern Ontario, Canada. Am Orch Soc Bull. 49:237–244.
  • Hosomi ST, Custódio CC, Seaton PT, Marks TR, Machado-Neto NB. 2012. Improved assessment of viability and germination of Cattleya (Orchidaceae) seeds following storage. In Vitro Cell Dev Biol-Plant. 48(1):127–136.
  • Hosomi ST, Santos RB, Custodio CC, Seaton PT, Marks TR, Machado-Neto NB. 2011. Preconditioning Cattleya seeds to improve the efficacy of the tetrazolium test for viability. Seed Sci Technol. 39(1):178–189.
  • Kendon JP, Rajaovelona L, Sandford H, Fang R, Bell J, Sarasan V. 2017. Collecting near mature and immature orchid seeds for ex situ conservation: “in vitro collecting” as a case study. Bot Stud. 58(1):34.
  • Lauzer D, St-Arnaud M, Barabé D. 1994. Tetrazolium staining and in vitro germination of mature seeds of Cypripedium acaule (Orchidaceae). Lindleyana. 9:197–204.
  • Magrini S, De Carli A, Onofri S, Scoppola A. 2012. A comparative study of the seed germination capabilities of Anacamptis palustris (Orchidaceae), a threatened terrestrial orchid, and other more common Anacamptis species, by asymbiotic culture in vitro. Eur J Environ Sci. 1(2):71–79.
  • Magrini S, De Vitis M. 2017. In vitro reproduction of three Limodorum species (Orchidaceae): impacts of scarification methods and nitrogen sources on mature seed germination and seedling development. Plant Biosyst. 151(3):419–428.
  • Magrini S, De Vitis M, Torelli D, Santi L, Zucconi L. 2019. Seed banking of terrestrial orchids: evaluation of seed quality in Anacamptis following 4-year dry storage. Plant Biol. DOI:10.1111/plb.12936.
  • Oliva AP, Arditti J. 1984. Seed germination of North American orchids. 11. Native California and related species of Aplectrum, Cypripedium, and Spiranthes. Bot Gaz. 145(4):495–501.
  • Pierce S, Spada A, Caporali E, Ceriani RM, Buffa G. 2019. Enzymatic scarification of Anacamptis morio (Orchidaceae) seed facilitates lignin degradation, water uptake and germination. Plant Biol. DOI:10.1111/plb.12694.
  • Ponert J, Vosolsobě S, Kmecová K, Lipavská H. 2012. European orchid cultivation – from seed to mature plant. Eur J Environ Sci. 1(2):95–107.
  • Pritchard HW. 1985. Determination of orchid seed viability using fluorescein diacetate. Plant Cell Environ. 8:727–730.
  • Rasmussen HN. 1995. Terrestrial orchids, from seed to mycotrophic plant. Cambridge (UK): Cambridge University Press; p. 444.
  • Seaton PT. 2007. Establishing a global network of orchid seed banks. Lankesteriana. 7:371–375.
  • Seaton PT, Hu H, Perner H, Pritchard HW. 2010. Ex situ conservation of orchids in a warming world. Bot Rev. 76(2):193–203.
  • Seaton PT, Pritchard HW. 2003. Orchid germplasm collection, storage and exchange. In: Dixon KW, Kell SP, Barrett RL and Cribb PJ, editors. Orchid conservation. Kota Kinabalu, Sabah, Malaysia: Natural History Publications (Borneo); pp. 227–258.
  • Seaton PT, Pritchard HW. 2011. Orchid seed stores for sustainable use: a model for future seed-banking activities. Lankesteriana. 11:349–353.
  • Sgarbi E, Del Prete C, Ronconi L, Dallai D. 2001. Asymbiotic micropropagation of wild Italian orchids: from seed to plant in a project for in situ reintroduction. J Eur Orch. 33:395–404.
  • Shoushtari BD, Heydary R, Jogson GL, Arditti J. 1994. Germination and viability staining of orchid seed following prolonged storage. Lindleyana. 9:77–84.
  • Singh F. 1981. Differential staining of orchid seeds for viability testing. Am Orch Soc Bull. 50(4):416–418.
  • Soares JS, Rosa YBCJ, Tatara MB, Sorgato JC, Lemes CSR. 2014. Identificação da viabilidade de sementes de orquídeas pelo teste de tetrazólio. [Viability identification of orchid seeds by the tetrazolium test]. Sem Ci Agr. 35:2275–2284.
  • Strober W. 2015. Trypan blue exclusion test of cell viability. Curr Protoc Immunol. 111:A3.B.1–A3.B.3.
  • Suzuki RM, Moreira VC, Pescador R, de Melo Ferreira W. 2012. Asymbiotic seed germination and in vitro seedling development of the threatened orchid Hoffmannseggella cinnabarina. In Vitro Cell Dev Biol-Plant. 48(5):500–511.
  • Swartz ND, Dixon KW. 2009. Terrestrial orchid conservation in the age of extinction. Ann Bot. 104(3):543–556.
  • Swartz ND, Dixon KW. 2017. Conservation methods for terrestrial orchids. USA: J. Ross Publishing; p. 235.
  • Thompson DI, Edwards TJ, van Staden J. 2001. In vitro germination of several South African summer rainfall Disa (Orchidaceae) species: is seed testa structure a function of habitat and a determinant of germinability? Syst Geogr Plants. 71(2):597–606.
  • Van der Kinderen G. 1987. Abscisic acid in terrestrial orchid seeds: a possible impact on their germination. Lindleyana. 2:84–87.
  • Waes JM, Debergh PC. 1986a. Adaptation of the tetrazolium method for testing the seed viability, and scanning electron microscopy study of some Western European orchids. Physiol Plant. 66(3):435–442.
  • Waes JM, Debergh PC. 1986b. In vitro germination of some Western European orchids. Physiol Plant. 67(2):253–261.
  • Verma P, Majee M. 2013. Seed germination and viability test in tetrazolium (TZ) assay. Bio-protoc. 3(17):e884.
  • Veyret Y. 1969. La structure des semences des Orchidaceae et leur aptitude à la germination in vitro en cultures pures. In: Travaux du Laboratorie de la Jaysinia. Vol.3. Paris: Laboratorie de Biologie Végétale du Muséum; pp. 89–98.
  • Vujanovic V, St AM, Barabé D, Thibeault G. 2000. Viability testing of orchid seed and the promotion of colouration and germination. Ann Bot. 86(1):79–86.
  • Yeung EC. 2017. A perspective on orchid seed and protocorm development. Bot Stud. 58(1):33
  • Zeng S, Zhang Y, Teixeira da Silva JA, Wu K, Zhang J, Duan J. 2014. Seed biology and in vitro seed germination of Cypripedium. Crit Rev Biotechnol. 34(4):358–371.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.