518
Views
5
CrossRef citations to date
0
Altmetric
Original Article

Enzyme immunoassay may be inadequate for measuring salivary testosterone in older men

&
Pages 415-423 | Received 11 Jul 2018, Accepted 04 Aug 2018, Published online: 07 Sep 2018

References

  • Diamanti-Kandarakis E, Dattilo M, Macut D, et al. Aging and anti-aging: a comboendocrinology overview. Eur J Endocrinol. 2017;176:R283–R308.
  • Welker K, Lassetter B, Brandes C, et al. A comparison of salivary testosterone measurement using immunoassays and tandem mass spectrometry. Psychoneuroendocrinology. 2016;71:180–188.
  • Dabbs J. Jr. Salivary testosterone measurements: reliability across hours, days, and weeks. Physiology & Behavior. 1990;48:83–86.
  • Wittert G. The relationship between sleep disorders and testosterone. Curr Opin Endocrinol Diabetes Obes. 2014;21:239–243.
  • Luboshitzky R, Shen-Orr Z, Herer P. Middle-aged men secrete less testosterone at night than young healthy men. J Clin Endocrinol Metab. 2003;88:3160–3166.
  • Derby CA, Zilber S, Brambilla D, et al. Body mass index, waist circumference and waist to hip ratio and change in sex steroid hormones: the Massachusetts Male Ageing Study. Clin Endocrinol. 2006;65:125–131.
  • Haring R, Ittermann T, Völzke H, et al. Prevalence, incidence and risk factors of testosterone deficiency in a population-based cohort of men: results from the study of health in Pomerania. Aging Male. 2010;13:247–257.
  • Wu F, Tajar A, Pye S, et al. Hypothalamic-pituitarytesticular axis disruptions in older men are differentially linked to age and modifiable risk factors: The European male aging study. J Clin Endocrin Metab. 2008;93:2737–2745.
  • Kim Y, Hong D, Lee D, et al. Total testosterone may not decline with ageing in Korean men aged 40 years or older. Oxf Bull Econ Stat. 2012;77:296–301.
  • Camacho E, Huhtaniemi I, O’Neil T, et al. Age-associated changes in hypothalamic–pituitary–testicular function in middle-aged and older men are modified by weight change and lifestyle factors: longitudinal results from the European Male Ageing Study. Eur J Endocrinol. 2013;168:445–455.
  • Kaplan SA, Lee JY, O’Neill EA, et al. Prevalence of low testosterone and its relationship to body mass index in older men with lower urinary tract symptoms associated with benign prostatic hyperplasia. Aging Male. 2013;16:169–172.
  • Shi Z, Araujo A, Martin S, et al. Longitudinal changes in testosterone over five years in community-dwelling men. J Clin Endocrinol Metab. 2013;98:3289–3297.
  • Bann D, Wu F, Keevil B, et al. Changes in testosterone related to body composition in late midlife: findings from the 1946 British birth cohort study. Obesity. 2015;23:1486–1492.
  • Saad F, Yassin A, Doros G, et al. Effects of long-term treatment with testosterone on weight and waist size in 411 hypogonadal men with obesity classes I-III: observational data from two registry studies. Int J Obes. 2016;40:162–170.
  • Clifton S, Macdowall W, Copas A, et al. Salivary testosterone levels and health status in men and women in the British general population: findings from the Third National Survey of Sexual Attitudes and Lifestyles (Natsal-3). J Clin Endocrinol Metab. 2016;101:3939–3951.
  • Yassin A, Nettleship JE, Talib RA, et al. Effects of testosterone replacement therapy withdrawal and re-treatment in hypogonadal elderly men upon obesity, voiding function and prostate safety parameters. Aging Male. 2016;19:64–69.
  • Salman M, Yassin DJ, Shoukfeh H, et al. Early weight loss predicts the reduction of obesity in men with erectile dysfunction and hypogonadism undergoing long-term testosterone replacement therapy. Aging Male. 2017;20:45–48.
  • Vermeulen A, Goemaere S, Kaufman J. Testosterone, body compositionand aging. J Endocrinol Invest. 1999;22(Suppl):110–116.
  • Travison T, Araujo A, O’Donnell A, et al. A population-level decline in serum testosterone levels in American men. J Clin Endocrinol Metab. 2006;92:196–202.
  • Andersson A, Jensen T, Juul A, et al. Secular decline in male testosterone and sex hormone binding globulin serum levels in Danish population surveys. J Clin Endoc Metab. 2007;92:4696–4705.
  • Liu PY, Beilin J, Meier C, et al. Age-related changes in serum testosterone and sex hormone binding globulin in Australian men: longitudinal analyses of two geographically separate regional cohorts. J Clin Endocrinol Metab. 2007;92:3599–3603.
  • Mazur A. The age-testosterone relationship in Black, White and Mexican-American men, and reasons for ethnic differences. Aging Male. 2009;12:66–76.
  • Yeap B, Araujo AB, Wittert GA. Do low testosterone levels contribute to ill-health during male ageing? Cri Rev Clin Lab Sci. 2012;49:168–182.
  • Corona G, Rastrelli G, Morgentaler A, et al. Meta-analysis of results of testosterone therapy on sexual function based on international index of erectile function scores. Eur Urol. 2017;72:1000–1011.
  • Rizk PJ, Kohn TP, Pastuszak AW, et al. Testosterone therapy improves erectile function and libido in hypogonadal men. Curr Opin Urol. 2017;27:511–515.
  • Rosen RC, Wu F, Behre HM, et al. Quality of life and sexual function benefits of long-term testosterone treatment: longitudinal results from the registry of hypogonadism in men (rhyme. J Sex Med. 2017;14:1104–1115.
  • Ajo R, Segura A, Mira L. The relationship of salivary testosterone and male sexual dysfunction in opioid-associated androgen deficiency (OPIAD). Aging Male. 2017;20:1–8.
  • Almehmadi Y, Yassin DJ, Yassin AA. Erectile dysfunction is a prognostic indicator of comorbidities in men with late onset hypogonadism. Aging Male. 2015;18:186–194.
  • Trinick TR, Feneley MR, Welford H, et al. International web survey shows high prevalence of symptomatic testosterone deficiency in men. Aging Male. 2011;14:10–15.
  • La Vignera S, Condorelli R, Vicari E, et al. Original immunophenotype of blood endothelial progenitor cells and microparticles in patients with isolated arterial erectile dysfunction and late onset hypogonadism: effects of androgen replacement therapy. Aging Male. 2011;14:183–189.
  • Aslan Y, Guzel O, Balci M, et al. The impact of metabolic syndrome on serum total testosterone level in patients with erectile dysfunction. Aging Male. 2014;17:76–80.
  • Carruthers M, Cathcart P, Feneley MR. Evolution of testosterone treatment over 25 years: symptom responses, endocrine profiles and cardiovascular changes. Aging Male. 2015;18:217–227.
  • Shigehara K, Konaka H, Ijima M, et al. The correlation between highly sensitive C-reactive protein levels and erectile function among men with late-onset hypogonadism. Aging Male. 2016;19:239–243.
  • Kulej-Lyko K, Majda J, von Haehling S, et al. Could gonadal and adrenal androgen deficiencies contribute to the depressive symptoms in men with systolic heart failure? Aging Male. 2016;19:221–230.
  • Wolf J, Keipert D, Motazedi H, et al. Effectiveness and tolerability of parenteral testosterone undecanoate: a post-marketing surveillance study. Aging Male. 2017;20:225–234.
  • Kaya E, Sikka SC, Kadowitz PJ, et al. Aging and sexual health: getting to the problem. Aging Male. 2017;20:65–80.
  • Panach-Navarrete J, Martínez-Jabaloyas JM, DE-SDT study group. The influence of comorbidities on the aging males’ symptoms scale in patients with erectile dysfunction. Aging Male. 2017;20:146–152.
  • Handelsman DJ. Testosterone and male aging: faltering hope for rejuvenation. JAMA. 2017;317:699–701.
  • Finkle WD, Greenland S, Ridgeway GK, et al. Increased risk of non-fatal myocardial infarction following testosterone therapy prescription in men. PLoS ONE 2014;9:e85805.
  • Vigen R, O’Donnell C, Barón A, et al. Association of testosterone therapy with mortality, myocardial infarction, and stroke in men with low testosterone levels. JAMA. 2013;310:1829–1836.
  • Araujo A, Dixon J, Suarez E, et al. Clinical review: endogenous testosterone and mortality in men: a systematic review and metaanalysis. J Clin Endocrinol Metab. 2011;96:3007–3019.
  • Shores MM, Smith NL, Forsberg CW, et al. Testosterone treatment and mortality in men with low testosterone levels. J Clin Endocrinol Metab. 2012;97:2050–2058.
  • Blaya R, Thomaz LD, Guilhermano F, et al. Total testosterone levels are correlated to metabolic syndrome components. Aging Male. 2016;19:85–89.
  • Pastuszak AW, Kohn TP, Estis J, et al. Low plasma testosterone is associated with elevated cardiovascular disease biomarkers. J Sex Med. 2017;14:1095–1103.
  • Selvin E, Feinleib M, Zhang L, et al. Androgens and diabetes in men: results from the Third National Health and Nutrition Examination Survey (NHANES III). Diabetes Care. 2007;30:234–238.
  • Haider A, Yassin A, Doros G, et al. Effects of long-term testosterone therapy on patients with “diabesity’’: results of observational studies of pooled analyses in obese hypogonadal men with type 2 diabetes. Int J Endocrinol. 2014;2014:683515.
  • Yassin DJ, Doros G, Hammerer PG, et al. Long-term testosterone treatment in elderly men with hypogonadism and erectile dysfunction reduces obesity parameters and improves metabolic syndrome and health-related quality of life. J Sex Med. 2014;11:1567–1576.
  • Mazur A, Westerman R, Mueller U. Is rising obesity causing a secular (age-independent) decline in testosterone among American men? PLoSONE. 2013;8:e76178.
  • Saad F, Yassin A, Almehmadi Y, et al. Effects of long-term testosterone replacement therapy, with a temporary intermission, on glycemic control of nine hypogonadal men with type 1 diabetes mellitus - a series of case reports. Aging Male. 2015;18:164–168.
  • Erens B, Phelps A, Clifton S, et al. Methodology of the third British National Survey of Sexual Attitudes and Lifestyles (Natsal-3). Sex Transm Infect. 2014;90:84–89.
  • Waite LJ, EO, Laumann W, Levinson ST, et al. 2007. National Social Life, Health, and Aging Project (NSHAP). ICPSR20541-v1. Chicago, IL: National Opinion Research Center.
  • Shirtcliff EA, Granger DA, Likos A, et al. Gender differences in the validity of testosterone measured in saliva by immunoassay. Horm Behav. 2002;42:62–69.
  • Keevil BG, MacDonald P, Macdowall W, et al. Salivary testosterone measurement by liquid chromatography tandem mass spectrometry in adult males and females. Ann Clin Biochem Int J Biochem Lab Med. 2014;51:368–378.
  • Fiers T, Delanghe J, T'Sjoen G, et al. A critical evaluation of salivary testosterone as a method for the assessment of serum testosterone. Steroids. 2014;86:5–9.
  • Mendoza K, MJ, Curran, ST. Lindau 2007. [internet] Salivary Testosterone Measurement in Wave I of the National Social Life, Health and Aging Project (NSHAP). Chicago Core on Biomarkers in Population-Based Aging Research. [cited 2007 Dec 17]. Available from: http://biomarkers.uchicago.edu/pdfs/TR-Testosterone.pdf
  • Gavrilova N, Lindau S. Salivary sex hormone measurement in a national, population-based study of older adults. J Gerontol B. 2009;64B:i94–i105.
  • Kozloski M, Schumm L, McClintock M. The utility and dynamics of Salivary sex hormone measurements in the national social life, health, and aging project, wave 2. J Gerontol B Psychol Sci Soc Sci. 2014;69:S215–S228.
  • Macdonald P, Owen L, Wu F, et al. A liquid chromatography–tandem mass spectrometry method for salivary testosterone with adult male reference interval determination. Clin Chem. 2011;57:774–775.
  • Haring R, Hannemann A, John U, et al. Age-specific reference ranges for serum testosterone and androstenedione concentrations in women measured by liquid chromatography-tandem mass spectrometry. J Clin Endocrinol Metab. 2012;97:408–415.
  • Davison L, Bell R, Donath S, et al. Androgen levels in adult females: changes with age, menopause, and oophorectomy. J Clin Endocrinol Metab. 2005;90:3847–3853.
  • Keevil B, Clifton S, Tanton C, et al. Distribution of salivary testosterone in men and women in a British general population-based sample: the Third National Survey of Sexual Attitudes and Lifestyles (Natsal-3). J Endocrine Society. 2017;1:14–25.
  • Handelsman D, Wartofsky L. Requirement for mass spectrometry sex steroid assays in the Journal of Clinical Endocrinology and Metabolism. J Clin Endocrinol Metab. 2013;98:3971–3973.
  • Mazur A. History of Biobehavioral Endocrinology in Humans. In: P. Mehta and O. Schultheiss, editors. International Handbook of Social Neuroendocrinology. London: Routledge; 2018.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.