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Original

Biology of cord blood cells and future prospects for enhanced clinical benefit

Pages 209-218 | Published online: 07 Jul 2009

References

  • Gluckman E, Broxmeyer HE, Auerbach AD, et al. Hematopoietic reconstitution in a patient with Fanconi anemia by means of umbilical-cord blood from an HLA-identical sibling. N Engl J Med 1989; 321: 1174–8
  • Broxmeyer HE, Kurtzberg J, Gluckman E, et al. Umbilical cord blood hematopoietic stem and repopulating cells in human clinical transplantation. Blood Cells 1991; 17: 313–29
  • Broxmeyer HE, Gluckman E, Auerbach A, et al. Human umbilical cord blood: a clinically useful source of transplantable hematopoietic stem/progenitor cells. Int J Cell Cloning 1991; 8(suppl 1)76–91
  • Broxmeyer HE. Introduction: past, present and future of cord blood transplantation. Cellular Characteristics of Cord Blood and Cord Blood Transplantation, HE Broxmeyer. American Association of Blood Banking, Bethesda, MD 1997; 1–11
  • Broxmeyer HE. Introduction. Cord blood transplantation: looking back and to the future. Cord Blood Characteristics: Role in Stem Cell Transplantation, SBA Cohen, E Gluckman, P Rubinstein, JA Madrigal. M Dunitz, London 2000; 1–12
  • Steinbrook R. The cord-blood-bank controversies. N Engl J Med 2004; 22: 2255–7
  • Rubinstein P, Carrier C, Scaradavou A, et al. Outcomes among 562 recipients of placental-blood transplants from unrelated donors. N Engl J Med 1998; 339: 1565–77
  • Broxmeyer HE, Douglas GW, Hangoc G, et al. Human umbilical cord blood as a potential source of transplantable hematopoietic stem/progenitor cells. Proc Natl Acad Sci USA 1989; 86: 3828–32
  • Broxmeyer HE, Hangoc G, Cooper S, et al. Growth characteristics and expansion of human umbilical cord blood and estimation of its potential for transplantation of adults. Proc Natl Acad Sci USA 1992; 89: 4109–13
  • Laughlin MJ, Barker J, Bambach B, et al. Hematopoietic engraftment and survival in adult recipients of umbilical-cord blood from unrelated donors. N Engl J Med 2001; 334: 1815–22
  • Long GD, Laughlin M, Madan B, et al. Unrelated umbilical cord blood transplantation in adult patients. Biol Blood Marrow Transpl 2003; 9: 772–80
  • Laughlin MJ, Eapen M, Rubinstein P, et al. Outcomes after transplantation of cord blood or bone marrow from unrelated donors in adults with leukemia. N Engl J Med 2004; 351: 2265–75
  • Rocha V, Labopin M, Sanz G, et al. Transplants of umbilical-cord blood or bone marrow from unrelated donors in adults with acute leukemia. N Engl J Med 2004; 351: 2276–85
  • Sanz M. Cord-blood transplantation in patients with leukemia: a real alternative for adults. N Engl J Med 2004; 351: 2328–30
  • Bodine DM. Animal models for the engraftment and differentiation of human hematopoietic stem and progenitor cells. Cord Blood: Biology, Immunology, Banking, and Clinical Transplantation, HE Broxmeyer. American Association of Blood Banking, Bethesda, MD 2004; 47–64
  • Vormoor J, Lapidot T, Pflumio F, et al. Immature human cord blood progenitors engraft and proliferate to high levels in immune-deficient SCID mice. Blood 1994; 83: 2489–97
  • Orazi A, Braun SE, Broxmeyer HE. Immunohistochemistry represents a useful tool to study human cell engraftment in SCID mice transplantation models. Blood Cells 1994; 20: 323–30
  • Bock TA, Orlic D, Dunbar CE, et al. Improved engraftment of human hematopoietic cells in severe combined immunodeficient (SCID) mice carrying human cytokine transgenes. J Exp Med 1995; 182: 2037–43
  • Lowry PA, Shultz LD, Greiner DL, et al. Improved engraftment of human cord blood stem cells in NOD/LtSz-scid/scid mice after irradiation or multiple-day injections into unirradiated patients. Blood Marrow Transplant 1996; 2: 15–23
  • Larochelle A, Vormoor J, Hanenberg H, et al. Identification of primitive human hematopoietic cells capable of repopulating NOD/SCID mouse bone marrow: implications for gene therapy. Nat Med 1996; 2: 1329–37
  • Bhatia M, Wang JCY, Kapp U, et al. Purification of primitive human hematopoietic cells capable of repopulating immune-deficient mice. Proc Natl Acad Sci USA 1997; 94: 5320–5
  • Hogan CJ, Shpall EJ, McNulty O, et al. Engraftment and development of human CD34+-enriched cells from umbilical cord blood in NOD/LtSz-scid/scid mice. Blood 1997; 90: 85–96
  • Hogan CJ, Shpall EJ, McNiece I, et al. Multilineage engraftment in NOD/LtSz-scis/scid mice from mobilized human CD34+ peripheral blood progenitor cells. Biol Blood Marrow Transpl 1997; 3: 236–46
  • Wang JCY, Doedens M, Dick JE. Primitive human hematopoietic cells are enriched in cord blood compared with adult bone marrow or mobilized peripheral blood as measured by the quantitative in vivo SCID-repopulating cell assay. Blood 1997; 89: 3919–24
  • Ueda T, Yoshida M, Yoshino H, et al. Hematopoietic capability of CD34+ cord blood cells: a comparison with CD34+ adult bone marrow cells. Int J Hematol 2001; 73: 457–62
  • Christopherson KW, II, Hangoc G, Mantel C, et al. Modulation of hematopoietic stem cell homing and engraftment by CD26. Science 2004; 305: 1000–3
  • Wang J, Kimura T, Asada R, et al. SCID-repopulating cell activity of human cord blood-derived CD34− cells assured by intra-bone marrow injection. Blood 2003; 101: 2924–31
  • Mazurier F, Doedens M, Gan OI, et al. Rapid myeloerythroid repopulation after intrafemoral transplantation of NOD-SCID mice reveals a new class of human stem cells. Nature Med 2003; 9: 959–63
  • Barker JN, Weisdorf DJ, Wagner JE. Creation of a double chimera after transplantation of umbilical-cord blood from two partially matched unrelated donors. N Engl J Med 2001; 344: 1870–1
  • Barker JN, Weisdorf DJ, DeFor TE, et al. Transplantation of two partially HLA-matched umbilical cord blood units to enhance engraftment in adults with hematological malignancies. Blood 2005; 105: 1343–7
  • Shpall EJ, Quinones R, Giller R, et al. Transplantation of ex vivo expanded cord blood. Biol Bone Marrow Transpl 2002; 8: 368–76
  • Jaroscak J, Goltry K, Smith A, et al. Augmentation of umbilical cord blood (UCB) transplantation with ex-vivo-expanded UCB cells: results of a phase I trial using the AstromReplicell system. Blood 2003; 101: 5061–7
  • Broxmeyer HE. Proliferation, self-renewal, and survival characteristics of cord blood hematopoietic stem and progenitor cells. Cord Blood: Biology, Immunology, Banking, and Clinical Transplantation, HE Broxmeyer. American Association of Blood Banking, Bethesda, MD 2004; 1–21
  • Smith FO, Srour EF, Broxmeyer HE. Ex-vivo expansion and gene transduction of cord blood stem cells. Cord Blood: Biology, Immunology, Banking, and Clinical Transplantation, HE Broxmeyer. American Association of Blood Banking, Bethesda, MD 2004; 125–50
  • Shaheen M, Broxmeyer HE. The humoral regulation of hematopoiesis. Hematology: Basic Principles and Practice4th Edition, R Hoffman, E Benz, S Shattil, B Furie, H Cohen, L Silberstein, P McGlave, 2005; 233–65
  • McNiece IK, Almeida-Porada G, Shpall EJ, et al. Ex vivo expanded cord blood cells provide rapid engraftment in fetal sheep but lack long-term engrafting potential. Exp Hematol 2002; 30: 612–6
  • Lewis ID, Almeida-Porada G, Du J, et al. Umbilical cord blood cells capable of engrafting in primary, secondary, and tertiary xenogeneic hosts are preserved after ex vivo culture in a noncontact system. Blood 2001; 97: 3441–9
  • Milner LA, Kopan R, Martin DIK, et al. A human homologue of the Drosophila developmental gene, notch, is expressed in CD34+ hematopoietic precursors. Blood 1994; 83: 2057–62
  • Varnum-Finney B, Purton LE, Yu M, et al. The notch ligand, jagged-1, influences the development of primitive hematopoietic precursor cells. Blood 1998; 91: 4084–91
  • Stier S, Cheng T, Dombkowski D, et al. Notch1 activation increases hematopoietic stem cell self-renewal in vivo and favors lymphoid over myeloid lineage outcome. Blood 2002; 99: 2369–78
  • Ohishi K, Varnum-Finney B, Berstein ID. Delta-1 enhances marrow and thymus repopulating ability of human CD34+CD38− cord blood cells. J Clin Invest 2002; 110: 1165–74
  • Varnum-Finney B, Brashem-Stein C, Bernstein ID. Combined effects of Notch signaling and cytokines induce a multiple log increase in precursors with lymphoid and myeloid reconstituting ability. Blood 2003; 101: 1784–9
  • Austin TW, Solar GP, Ziegler FC, et al. A role for Wnt gene family in hematopoiesis: expression of multilineage progenitor cells. Blood 1997; 89: 3624–35
  • Van DerBerg DJ, Sharma AK, Bruno E, et al. Role of members of the Wnt gene family in human hematopoiesis. Blood 1998; 92: 3189–202
  • Reya T, Duncan AW, Ailles L, et al. A role for Wnt signaling in self-renewal of haematopoietic stem cells. Nature 2003; 423: 409–14
  • Willert K, Brown JD, Danenberg E, et al. Wnt proteins are lipid-modified and can act as stem cell growth factors. Nature 2003; 423: 448–52
  • Antonchuk J, Sauvageau G, Humphries RK. HOXB4 overexpression mediates very rapid stem cell regulation and competitive hematopoietic repopulation. Exp Hematol 2001; 29: 1125–34
  • Buske C, Feuring-Buske M, Abramovich C, et al. Deregulated expression of HOXB4 enhances the primitive growth activity of human hematopoietic cells. Blood 2002; 100: 862–8
  • Antonchuk J, Sauvageau G, Humphries RK. HOXB4-induced expansion of adult hematopoietic stem cells ex vivo. Cell 2002; 109: 39–45
  • Kyba M, Perlingeiro RCR, Daley GQ. HoxB4 confers definitive lymphoid-myeloid engraftment potential on embryonic stem cell and yolk sac hematopoietic progenitors. Cell 2002; 109: 29–37
  • Krosi J, Beslu N, Mayotte N, et al. The competitive nature of HOXB4-transduced HSC is limited by PBX1: the generation of ultra-competitive stem cells retaining full differentiation potential. Immunity 2003; 18: 561–71
  • Krosl J, Austin P, Beslu N, et al. In vitro expansion of hematopoietic stem cells by recombinant TAT-HOXB4 protein. Nature Med 2003; 9: 1428–32
  • Amsellem S, Pflumio F, Bardinet D, et al. Ex vivo expansion of human hematopoietic stem cells by direct delivery of the HOXB4 homeoprotein. Nature Med 2003; 9: 1423–7
  • Park I-K, Qian D, Kiel M, et al. Bmi-1 is required for maintenance of adult self-renewing haematopoietic stem cells. Nature 2003; 423: 302–5
  • Lessard J, Sauvageau G. Bmi-1 determines the proliferative capacity of normal and leukaemic stem cells. Nature 2003; 423: 255–60
  • Iwama A, Oguro H, Negishi M, et al. Enhanced self-renewal of hematopoietic stem cells mediated by the polycomb gene product Bmi-1. Immunity 2004; 21: 843–51
  • Zhang P, Iwasaki-Arai J, Iwasaki H, et al. Enhancement of hematopoietic stem cell repopulating capacity and self-renewal in the absence of the transcription factor C/EBPα. Immunity 2004; 21: 853–63
  • Hock H, Hamblen MJ, Rooke HM, et al. Gfi-1 restricts proliferation and preserves functional integrity of haematopoietic stem cells. Nature 2004; 431: 1002–7
  • Zeng H, Yücel R, Kosan C, et al. Transcription factor Gfi1 regulates self-renewal and engraftment of hematopoietic stem cells. EMBO J 2004; 23: 4116–25
  • McNiece I. Ex vivo expansion of hematopoietic cells: Editorial. Exp Hematol 2004; 32: 409–13
  • Phillips RL, Ernst RE, Brunk B, et al. The genetic program of hematopoietic stem cells. Science 2000; 288: 1635–40
  • Terskikh AV, Miyamoto T, Chang C, et al. Gene expression analysis of purified hematopoietic stem cells and committed progenitors. Blood 2003; 102: 94–101
  • Ramalho-Santos M, Yoon S, Matsuzaki Y, et al. ‘Stemness’: transcriptional profiling of embryonic and adult stem cells. Science 2002; 298: 597–600
  • Ivanova NB, Dimos JT, Schaniel C, et al. A stem cell molecular signature. Science 2002; 298: 601–4
  • Ma X, Husain T, Peng H, et al. Development of a murine hematopoietic progenitor complementary DNA microarray using a subtracted complementary DNA library. Blood 2002; 100: 833–44
  • Lambert JF, Liu M, Colvin GA, et al. Marrow stem cells shift gene expression and engraftment phenotype with cell cycle transit. J Exp Med 2003; 197: 1563–72
  • Park IK, He Y, Lin F, et al. Differential gene expression profiling of adult murine hematopoietic stem cells. Blood 2002; 99: 488–98
  • Tao W, Hangoc G, Hawes JW, et al. Profiling of differentially expressed apoptosis-related genes by cDNA arrays in human cord blood CD34+ cells treated with VP-16 etoposide. Exp Hematol 2003; 31: 251–60
  • Tao W, Broxmeyer HE. Towards a molecular understanding of hematopoietic stem and progenitor cells. Cord Blood: Biology, Immunology, Banking, and Clinical Transplantation, HE Broxmeyer. American Association of Blood Banking, Bethesda, MD 2004; 87–123
  • Tao W, Wang M, Voss E.D, et al. Comparative proteomic analysis of human CD34+ stem/progenitor cells and mature CD15+ myeloid cells. Stem Cells 2004; 22: 1003–14
  • Broxmeyer HE, Srour EF, Hangoc G, et al. High efficiency recovery of hematopoietic progenitor cells with extensive proliferative and ex-vivo expansion activity and of hematopoietic stem cells with NOD/SCID mouse repopulation ability from human cord blood stored frozen for 15 years. Proc Natl Acad Sci USA 2002; 100: 645–50
  • Traggiai E, Chicha L, Mazzucchelli L, et al. Development of a human adaptive immune system in cord blood cell-transplanted mice. Science 2004; 304: 104–7
  • Javazon EH, Beggs KJ, Flake AW. Mesenchymal stem cells: paradoxes of passaging. Exp Hematol 2004; 32: 414–25
  • Erices A, Conget P, Minguell JJ. Mesenchymal progenitor cells in human umbilical cord blood. Br J Haematol 2000; 109: 235–42
  • Goodwin HS, Bicknese AR, Chien SN, et al. Multilineage differentiation activity by cells isolated from umbilical cord blood: expression of bone, fat and neural markers. Biol Blood Marrow Transpl 2001; 7: 581–8
  • Bieback K, Kern S, Kluter H, et al. Critical parameters for the isolation of mesenchymal stem cells from umbilical cord blood. Stem Cells 2004; 22: 625–34
  • Yang S-E, Ha C-W, Jung MH, et al. Mesenchymal stem/progenitor cells developed in cultures from UC blood. Cytotherapy 2004; 6: 476–86
  • Lee OK, Kuo TK, Chen W-M, et al. Isolation of multipotent mesenchymal stem cells from umbilical cord blood. Blood 2004; 103: 1669–75
  • Rosada C, Justesen J, Melsvik D, et al. The human umbilical cord blood: a potential source for osteoblast progenitor cells. Calcif Tissue Int 2003; 72: 135–42
  • Bonanno G, Perillo A, Rutella S, et al. Clinical isolation and functional characterization of cord blood CD133+ hematopoietic progenitor cells. Transfusion 2004; 44: 1087–97
  • Gutierrez-Rodriguez M, Reyes-Maldonado E, Mayani H. Characterization of the adherent cells developed in Dexter type long-term cultures from human umbilical cord blood. Stem Cells 2000; 18: 45–52
  • Mareschi K, Biasin E, Piacibello W, et al. Isolation of human mesenchymal stem cells: bone marrow versus umbilical cord blood. Haematologica 2001; 86: 1099–100
  • Wexler S, Donaldson C, Denning-Kendall P, et al. Adult bone marrow is a rich source of human mesenchymal ‘stem’ cells but umbilical cord and mobilized adult blood are not. Br J Haematol 2003; 121: 368–74
  • Lee MW, Choi J, Yang MS, et al. Mesenchymal stem cells from cryopreserved human umbilical cord blood. Biochem Biophys Res Comm 2004; 320: 273–8
  • Hou L, Cao H, Wang D, et al. Induction of umbilical cord blood mesenchymal stem cells into neuron-like cells in vitro. Int J Hematol 2003; 78: 256–61
  • Gang EJ, Jeong JA, Hong SH, et al. Skeletal myogenic differentiation of mesenchymal stem cells isolated from human umbilical cord blood. Stem Cells 2004; 22: 617–24
  • Wang J-F, Wang L-J, Wu Y-F, et al. Mesenchymal stem/progenitor cells in human umbilical cord blood as support for ex vivo expansion of CD34+ hematopoietic stem cells and for chondrogenic differentiation. Haematologica 2004; 89: 837–44
  • Deans RJ, Moseley AB. Mesenchymal stem cells: biology and potential clinical uses. Exp Hematol 2000; 28: 875–84
  • Reyes M, Lund T, Lenvik T, et al. Purification and ex-vivo expansion of postnatal human marrow mesodermal progenitor cells. Blood 2001; 98: 2615–25
  • Jiang Y, Jahagirdar BN, Reinhardt RL, et al. Pluripotency of mesenchymal stem cells derived from adult marrow. Nature 2002; 418: 41–9
  • Schwartz RE, Reyes M, Koodie L, et al. Multipotent adult progenitor cells from bone marrow differentiate into functional hepatocyte-like cells. J Clin Invest 2002; 109: 1291–1302
  • Jiang Y, Vaessen B, Lenvik T, et al. Multipotent progenitor cells can be isolated from postnatal murine bone marrow, muscle, and brain. Exp Hematol 2002; 30: 896–904
  • Reyes M, Dudek A, Jahagirdar B, et al. Origin of endothelial progenitors in human postnatal bone marrow. J Clin Invest 2002; 109: 337–46
  • Adassi, A, Verfaille, CM. Multipotent adult progenitor cells. In:. R Lanza, Blau, H, Melton, D, Mooree, M, Thomas, ED, Verfaile, C, Weissman, I, West, M, eds. Handbook of Stem Cells, Volume 2. Adult and Fetal Cells. Amsterdam: Elsevier Academic Press, 2004:293–7.
  • Kogler G, Sensken S, Airey J, et al. A new human somatic stem cell from placental cord blood with intrinsic pluripotent differentiation potential. J Exp Med 2004; 200: 123–35
  • Rafii S, Lyden D. Therapeutic stem and progenitor cell transplantation for organ vascularization and regeneration. Nature Med 2003; 9: 702–12
  • Crisa L, Cirulli V, Smith K, et al. Human cord blood progenitors sustain thymic T-cell development and a novel form of angiogenesis. Blood 1999; 94: 3928–40
  • Peichev M, Naiyer A, Pereira D, et al. Expression of VEGFR-2 and AC133 by circulating human CD34+ cells identifies a population of functional endothelial precursors. Blood 2000; 95: 952–8
  • Kang HJ, Kim SC, Kim YJ, et al. Short-term phytohaemagglutinin-activated mononuclear cells induce endothelial progenitor cells from cord blood CD34− cells. Br J Haematol 2001; 113: 962–9
  • Pesce M, Orlandi A, Iachininoto MG, et al. Myoendothelial differentiation of human umbilical cord blood-derived stem cells in ischemic limb tissues. Circ Res 2003; 93: 51–62
  • Eggermann J, Kliche S, Jarmy G, et al. Endothelial progenitor cell culture and differentiation in vitro: a methodological comparison using human umbilical cord blood. Cardiovascular Res 2003; 58: 478–86
  • Fan C-L, Li Y, Gao P-J, et al. Differentiation of endothelial progenitor cells from human umbilical cord blood CD 34+ cells in vitro. Acta Pharmacol Sin 2003; 24: 212–8
  • Murga M, Yao L, Tosato G. Derivation of endothelial cells from CD34− umbilical cord blood. Stem Cells 2004; 22: 385–95
  • Hildbrand P, Cirulli V, Prinsen RC, et al. The role of angiopoietins in the development of endothelial cells from cord blood CD34+ progenitors. Blood 2004; 107: 2010–19
  • Ingram D, Mead L, Tanaka H, et al. Identification of a novel hierarchy of endothelial progenitor cells using human peripheral and umbilical cord blood. Blood 2004; 104: 2752–60
  • Aoki M, Yasutake M, Murohara T. Derivation of functional endothelial progenitor cells from human umbilical card blood mononuclear cells isolated by a novel cell filtration device. Stem Cells 2004; 22: 994–1002
  • Bompais H, Chagraoui J, Canron X, et al. Human endothelial cells derived from circulating progenitors display specific functional properties compared with mature vessel wall endothelial cells. Blood 2004; 107: 2577–84
  • Wagers A, Weissman I. Plasticity of adult stem cells. Cell 2004; 116: 639–48
  • Verfaillie, C. ‘Adult’ stem cells: tissue specific or not?, In:. R Lanza, Blau, H, Melton, D, Mooree, M, Thomas, ED, Verfaile, C, Weissman, I, West, M, eds. Handbook of Stem Cells, Volume 2. Adult and Fetal Cells. Amsterdam: Elsevier Academic Press, 2004:13–20.
  • Kucia M, Ratajczak J, Reca R, et al. Tissue-specific muscle, neural and liver stem/progenitor cells reside in the bone marrow, respond to an SDF-1 gradient and are mobilized into peripheral blood during stress and tissue injury. Blood Cells Molecules and Diseases 2004; 32: 52–7
  • Ratajczak MZ, Kucia M, Reca R, et al. Stem cell plasticity revisited: CXCR4-positive cells expressing mRNA for early muscle, liver and neural cells ‘hide out’ in the bone marrow. Leukemia 2004; 18: 29–40
  • Hough RA, Barke JN, Wagner JE. Innovation in umbilical cord blood transplantation. Cord Blood: Biology, Immunology, Banking, and Clinical Transplantation, HE Broxmeyer. American Association of Blood Banking, Bethesda, MD 2004; 333–75
  • Gluckman E, Rocha V. Cord blood transplantation in Europe: strategy of alternative donor transplant search. Cord Blood: Biology, Immunology, Banking, and Clinical Transplantation, HE Broxmeyer. American Association of Blood Banking, Bethesda, MD 2004; 403–28
  • Laughlin MJ. Cord blood transplantation for adults: hematopoietic stem cell biology and immunology. Cord Blood: Biology, Immunology, Banking, and Clinical Transplantation, HE Broxmeyer. American Association of Blood Banking, Bethesda, MD 2004; 403–20
  • Broxmeyer HE, Smith FO. Cord blood hematopoietic cell transplantation. Thomas Hematopoietic Cell Transplantation3rd Edition, KG Blume, SJ Forman, FR Appelbaum. Blackwell Scientific Publications, Cambridge, MA 2004; 550–64
  • Kim YJ, Broxmeyer HE. Cytotoxic T lymphocytes and umbilical cord blood transplantation. Cord Blood: Biology, Immunology, Banking, and Clinical Transplantation, HE Broxmeyer. American Association of Blood Banking, Bethesda, MD 2004; 199–217
  • Gluckman JC, Canque B. Dendritic cell and lymphoid progenitors in cord blood. Cord Blood: Biology, Immunology, Banking, and Clinical Transplantation, HE Broxmeyer. American Association of Blood Banking, Bethesda, MD 2004; 163–85
  • Navarrete CV, Gomez J, Borras FE. Cord blood dendritic cells. Cord Blood: Biology, Immunology, Banking, and Clinical Transplantation, HE Broxmeyer. American Association of Blood Banking, Bethesda, MD 2004; 187–98
  • Li, G, Kim, YJ, Broxmeyer, HE. Macrophage colony-stimulating factor drives cord blood monocyte differentiation into IL-10highIL-12absent dendritic cells with tolerogenic potential. J Immunol 2005;174:4706–17.
  • Jonuleit H, Schmidt E, Schuler G, et al. Induction of interleukin 10-producing, nonproliferating CD4+ T cells with regulatory properties by repetitive stimulation with allogeneic immature human dendritic cells. J Exp Med 2000; 192: 1213–22

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