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Journal of Enzyme Inhibition and Medicinal Chemistry Volume 22, 2007 - Issue 1
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Research Article

Inhibition by L-aspartol adenylate of a nondiscriminating aspartyl-tRNA synthetase reveals differences between the interactions of its active site with tRNAAsp and tRNAAsn.

Dominic Bernard
,
Pierre-Marie Akochy Département de Biochimie et de Microbiologie, and Centre de Recherche sur la Fonction, la Structure et l'Ingénierie des Protéines (CREFSIP), Université Laval, Québec (QC), Canada, G1K7P4
,
Stéphane Bernier Département de Chimie and Centre de Recherche sur la Fonction, la Structure et l'Ingénierie des Protéines (CREFSIP), Université Laval, Québec (QC), Canada, G1K7P4
,
Olivier Fisette Département de Biochimie et de Microbiologie, and Centre de Recherche sur la Fonction, la Structure et l'Ingénierie des Protéines (CREFSIP), Université Laval, Québec (QC), Canada, G1K7P4
,
Olivier Côté Brousseau Département de Biochimie et de Microbiologie, and Centre de Recherche sur la Fonction, la Structure et l'Ingénierie des Protéines (CREFSIP), Université Laval, Québec (QC), Canada, G1K7P4
,
Robert Chênevert Département de Chimie and Centre de Recherche sur la Fonction, la Structure et l'Ingénierie des Protéines (CREFSIP), Université Laval, Québec (QC), Canada, G1K7P4
,
Paul H. Roy
&
Jacques Lapointe Département de Biochimie et de Microbiologie, and Centre de Recherche sur la Fonction, la Structure et l'Ingénierie des Protéines (CREFSIP), Université Laval, Québec (QC), Canada, G1K7P4
 show all
Pages 77-82 | Received 12 Apr 2006, Accepted 10 Jul 2006, Published online: 04 Oct 2008

References

  • Woese CR, Olsen GJ, Ibba M, Soll D. Aminoacyl-tRNA synthetases, the genetic code, and the evolutionary process. Microbiol Mol Biol Rev 2000; 64: 202–236
  • Söll D, Ibba M. Aminoacyl-tRNA synthetase structure and evolution. Translation mechanisms, J Lapointe, L Brakier-Gingras. Kluwer academic/Plenum publishers, New York 2003; 25–33
  • Akochy PM, Bernard D, Roy PH, Lapointe J. Direct glutaminyl-tRNA biosynthesis and indirect asparaginyl-tRNA biosynthesis in Pseudomonas aeruginosa PAO1. J Bacteriol 2004; 186: 767–776
  • Feng L, Tumbula-Hansen D, Min B, Namgoong S, Salazar J, Orellane O, Söll D. Transfer RNA-dependent amidotransferases: key enzymes for Asn-tRNA and Gln-tRNA synthesis in nature. The Aminoacyl-tRNA synthetases, M Ibba, C Francklyn, S Cusack. Landes Bioscience, Georgetown, Texas 2005; 314–320
  • Kern D, Roy H, Becker HD. Asparaginyl-tRNA synthetases. The Aminoacyl-tRNA synthetases, M Ibba, C Francklyn, S Cusack. Landes Bioscience, Georgetown, Texas 2005; 193–209
  • Giegé R, Rees B. Aspartyl-tRNA synthetases. The Aminoacyl-tRNA synthetases, M Ibba, C Francklyn, S Cusack. Landes Bioscience, Georgetown, Texas 2005; 210–226
  • Giegé R, Frugier M. Transfer RNA structure and identity. Translation mechanisms, J Lapointe, L Brakier-Gingras. Kluwer academic/Plenum publishers, New York 2003; 1–24
  • Ryckelynck M, Giegé R, Frugier M. Yeast tRNA(Asp) charging accuracy is threatened by the N-terminal extension of aspartyl-tRNA synthetase. J Biol Chem 2003; 278: 9683–9690
  • Bernier S, Akochy PM, Lapointe J, Chênevert R. Synthesis and aminoacyl-tRNA synthetase inhibitory activity of aspartyl adenylate analogs. Bioorg Med Chem 2005; 13: 69–75
  • Sekine S, Nureki O, Dubois DY, Bernier S, Chênevert R, Lapointe J, Vassylyev DG, Yokoyama S. ATP binding by glutamyl-tRNA synthetase is switched to the productive mode by tRNA binding. EMBO J 2003; 22: 676–688
  • Chênevert R, Bernier S, Lapointe J. Inhibitors of aminoacyl-tRNA synthetases as antibiotics and tools for structural and mechanistic studies. Translation mechanisms, J Lapointe, L Brakier-Gingras. Kluwer academic/Plenum publishers, New York 2003; 416–428
  • Freist W, Gauss DH, Söll D, Lapointe. Glutamyl-tRNA sythetase. J Biol Chem 1997; 378: 1313–1329
  • Hong K-W, Ibba M, Weygand-Durasevic I, Rogers MJ, Thomann HU, Soll D. Transfer RNA-dependent cognate amino acid recognition by an aminoacyl-tRNA synthetase. EMBO J 1996; 15: 1983–1991
  • Ibba M, Losey HC, Kawarabayasi Y, Kikuchi H, Bunjun S, Soll D. Substrate recognition by class I lysyl-tRNA synthetases: a molecular basis for gene displacement. Proc Natl Acad Sci 1999; 96: 418–423, USA
  • Ibba M, Hong KW, Sherman JM, Sever S, Soll D. Interactions between tRNA identity nucleotides and their recognition sites in glutaminyl-tRNA synthetase determine the cognate amino acid affinity of the enzyme. Proc Natl Acad Sci 1996; 93: 6953–6958, USA
  • Moulinier L, Eiler S, Eriani G, Gangloff J, Thierry J-C, Gabriel K, McClain WH, Moras D. The structure of an AspRS-tRNA(Asp) complex reveals a tRNA-dependent control mechanism. EMBO J 2001; 20: 5290–5301
  • Becker HD, Giege R, Kern D. Identity of prokaryotic and eukaryotic tRNA(Asp) for aminoacylation by aspartyl-tRNA synthetase from Thermus thermophilus. Biochemistry 1996; 35: 7447–7458
  • Uter NT, Perona JJ. Long-range intramolecular signaling in a tRNA synthetase complex revealed by pre-steady-state kinetics. Proc Natl Acad Sci 2004; 101: 14396–14401, USA
  • Fersht A. Enzyme Structure and Mechanism2nd Edn. W.H. Freeman and Co., New York, NY 1984
  • Giegé R, Sissler M, Florentz C. Universal rules and idiosyncratic features in tRNA identity. Nucleic Acids Res 1998; 26: 5017–5035
  • Ibba M, Sever S, Praetorius-Ibba M, Söll D. Transfer RNA identity contributes to transition state stabilization during aminoacyl-tRNA synthesis. Nucleic Acids Res. 1999; 27: 3631–3637
  • Feng L, Yuan J, Toogood H, Tumbula-Hansen D, Söll D. Aspartyl-tRNA synthetase requires a conserved proline in the anticodon-binding loop for tRNAAsn recognition in vivo. J Biol Chem 2005; 280: 20638–20641
  • Fersht AR, Ashford JS, Bruton CJ, Jakes R, Koch GLE, Hartley BS. Active site titration and aminoacyl adenylate binding stoichiometry of aminoacyl-tRNA synthetases. Biochemistry 1975; 14: 1–4
  • Guez-Ivanier V, Hermann M, Baldwin D, Bedouelle H. Mapping the stability determinants of bacterial tyrosyl transfer RNA synthetases by an experimental evolutionary approach. J Mol Biol 1993; 234: 209–221
  • Cornish-Bowden A. Fundamentals of enzyme kinetics. Portland Press Ltd., London 1995

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