Comparison of rapid MMP-8 and interleukin-6 point-of-care tests to identify intra-amniotic inflammation/infection and impending preterm delivery in patients with preterm labor and intact membranes^*

References

• Kara M, Ozden S, Arioglu P, Cetin A. The significance of amniotic fluid interleukin-6 levels in preterm labour. Aust N Z J Obstet Gynaecol 1998;38:403–6.
   PubMed Web of Science ®Google Scholar
• Agrawal V, Hirsch E. Intrauterine infection and preterm labor. Semin Fetal Neonatal Med 2012;17:12–19.
   PubMed Web of Science ®Google Scholar
• Romero R, Dey SK, Fisher SJ. Preterm labor: one syndrome, many causes. Science 2014;345:760–65.
   PubMed Web of Science ®Google Scholar
• DiGiulio DB, Romero R, Amogan HP, et al. Microbial prevalence, diversity and abundance in amniotic fluid during preterm labor: a molecular and culture-based investigation. PLoS One 2008;3:e3056.
   PubMed Web of Science ®Google Scholar
• Romero R, Mazor M, Munoz H, et al. The preterm labor syndrome. Anne N Y Acad Sci 1994;734:414–29.
   PubMed Web of Science ®Google Scholar
• Cobo T, Kacerovsky M, Jacobsson B. Amniotic fluid infection, inflammation, and colonization in preterm labor with intact membranes. Am J Obstet Gynecol 2014;211:708.
   PubMed Web of Science ®Google Scholar
• Yoon BH, Romero R, Moon JB, et al. Clinical significance of intra-amniotic inflammation in patients with preterm labor and intact membranes. Am J Obstet Gynecol 2001;185:1130–36.
   PubMed Web of Science ®Google Scholar
• Shim SS, Romero R, Hong JS, et al. Clinical significance of intra-amniotic inflammation in patients with preterm premature rupture of membranes. Am J Obstet Gynecol 2004;191:1339–45.
   PubMed Web of Science ®Google Scholar
• Romero R, Gomez R, Chaiworapongsa T, et al. The role of infection in preterm labour and delivery. Paediatr Perinat Epidemiol 2001;15:41–56.
   PubMed Web of Science ®Google Scholar
• Park CW, Yoon BH, Kim SM, et al. The frequency and clinical significance of intra-amniotic inflammation defined as an elevated amniotic fluid matrix metalloproteinase-8 in patients with preterm labor and low amniotic fluid white blood cell counts. Obst Gynecol Sci 2013;56:167–75.
   PubMedGoogle Scholar
• Romero R, Miranda J, Chaiworapongsa T, et al. Prevalence and clinical significance of sterile intra-amniotic inflammation in patients with preterm labor and intact membranes. Am J Reprod Immunol 2014;72:458–74.
   PubMed Web of Science ®Google Scholar
• Musilova I, Kutova R, Pliskova L, et al. Intraamniotic inflammation in women with preterm prelabor rupture of membranes. PLoS One 2015;10:e0133929
   PubMed Web of Science ®Google Scholar
• Romero R, Mazor M, Wu YK, et al. Infection in the pathogenesis of preterm labor. Semin Perinatol 1988;12:262–79.
   PubMed Web of Science ®Google Scholar
• Romero R, Mazor M. Infection and preterm labor. Clin Obstet Gynecol 1988;31:553–84.
   PubMed Web of Science ®Google Scholar
• Romero R, Sirtori M, Oyarzun E, et al. Infection and labor. V. Prevalence, microbiology, and clinical significance of intraamniotic infection in women with preterm labor and intact membranes. Am J Obstet Gynecol 1989;161:817–24.
   PubMed Web of Science ®Google Scholar
• Gibbs RS, Romero R, Hillier SL, et al. A review of premature birth and subclinical infection. Am J Obstet Gynecol 1992;166:1515–28.
   PubMed Web of Science ®Google Scholar
• Goldenberg RL, Hauth JC, Andrews WW. Intrauterine infection and preterm delivery. N Engl J Med 2000;342:1500–7.
   PubMed Web of Science ®Google Scholar
• Romero R, Espinoza J, Chaiworapongsa T, Kalache K. Infection and prematurity and the role of preventive strategies. Semin Neonatol 2002;7:259–74.
   PubMedGoogle Scholar
• Goncalves LF, Chaiworapongsa T, Romero R. Intrauterine infection and prematurity. Ment Retard Dev Disabil Res Rev 2002;8:3–13.
   PubMedGoogle Scholar
• Romero R, Espinoza J, Goncalves LF, et al. Inflammation in preterm and term labour and delivery. Semin Fetal Neonatal Med 2006;11:317–26.
   PubMed Web of Science ®Google Scholar
• Romero R, Espinoza J, Goncalves LF, et al. The role of inflammation and infection in preterm birth. Semin Reprod Med 2007;25:21–39.
   PubMed Web of Science ®Google Scholar
• Vrachnis N, Vitoratos N, Iliodromiti Z, et al. Intrauterine inflammation and preterm delivery. Ann N Y Acad Sci 2010;1205:118–22.
   PubMed Web of Science ®Google Scholar
• Cobo T, Palacio M, Martinez-Terron M, et al. Clinical and inflammatory markers in amniotic fluid as predictors of adverse outcomes in preterm premature rupture of membranes. Am J Obstet Gynecol 2011;205:126 e1–8.
   PubMed Web of Science ®Google Scholar
• Gervasi MT, Romero R, Bracalente G, et al. Midtrimester amniotic fluid concentrations of interleukin-6 and interferon-gamma-inducible protein-10: evidence for heterogeneity of intra-amniotic inflammation and associations with spontaneous early (<32 weeks) and late (>32 weeks) preterm delivery. J Perinat Med 2012;40:329–43.
   PubMed Web of Science ®Google Scholar
• Watts DH, Krohn MA, Hillier SL, Eschenbach DA. The association of occult amniotic fluid infection with gestational age and neonatal outcome among women in preterm labor. Obstet Gynecol 1992;79:351–57.
   PubMed Web of Science ®Google Scholar
• Yoon BH, Romero R, Park JS, et al. Fetal exposure to an intra-amniotic inflammation and the development of cerebral palsy at the age of three years. Am J Obstet Gynecol 2000;182:675–81.
   PubMed Web of Science ®Google Scholar
• Hitti J, Tarczy-Hornoch P, Murphy J, et al. Amniotic fluid infection, cytokines, and adverse outcome among infants at 34 weeks' gestation or less. Obstet Gynecol 2001;98:1080–88.
   PubMed Web of Science ®Google Scholar
• Lawn JE, Cousens S, Zupan J. 4 million neonatal deaths: when? where? why? Lancet 2005;365:891–900.
   PubMed Web of Science ®Google Scholar
• Lee J, Oh KJ, Yang HJ, et al. The importance of intra-amniotic inflammation in the subsequent development of atypical chronic lung disease. J Matern Fetal Neonatal Med 2009;22:917–23.
   PubMed Web of Science ®Google Scholar
• Kramer BW, Kallapur S, Newnham J, Jobe AH. Prenatal inflammation and lung development. Semin Fetal Neonatal Med 2009;14:2–7.
   PubMed Web of Science ®Google Scholar
• Jobe AH, Kallapur SG. Chorioamnionitis, surfactant, and lung disease in very low birth weight infants. J Pediatr 2010;156:3–4.
   PubMed Web of Science ®Google Scholar
• Hofer N, Kothari R, Morris N, et al. The fetal inflammatory response syndrome is a risk factor for morbidity in preterm neonates. Am J Obstet Gynecol 2013;209:542 e1–11.
   PubMed Web of Science ®Google Scholar
• Kacerovsky M, Musilova I, Andrys C, et al. Prelabor rupture of membranes between 34 and 37 weeks: the intraamniotic inflammatory response and neonatal outcomes. Am J Obstet Gynecol 2014;210:325.e1–10.
   PubMed Web of Science ®Google Scholar
• Combs CA, Gravett M, Garite TJ, et al. Amniotic fluid infection, inflammation, and colonization in preterm labor with intact membranes. Am J Obstet Gynecol 2014;210:125 e1–15.
   PubMed Web of Science ®Google Scholar
• Combs CA, Gravett M, Garite TJ. Reply: To PMID 24274987. Am J Obstet Gynecol 2014;211:708–9.
   PubMed Web of Science ®Google Scholar
• Strunk T, Inder T, Wang X, et al. Infection-induced inflammation and cerebral injury in preterm infants. Lancet Infect Dis 2014;14:751–62.
   PubMed Web of Science ®Google Scholar
• Bastek JA, Weber AL, McShea MA, et al. Prenatal inflammation is associated with adverse neonatal outcomes. Am J Obstet Gynecol 2014;210:450.e1–e10.
   PubMed Web of Science ®Google Scholar
• Manuck TA, Varner MW. Neonatal and early childhood outcomes following early vs later preterm premature rupture of membranes. Am J Obstet Gynecol 2014;211:308 e1–6.
   PubMed Web of Science ®Google Scholar
• Manuck TA, Sheng X, Yoder BA, Varner MW. Correlation between initial neonatal and early childhood outcomes following preterm birth. Am J Obstet Gynecol 2014;210:426 e1–9.
   Web of Science ®Google Scholar
• Manuck TA, Rice MM, Bailit JL, Grobman WA, Reddy UM, Wapner RJ, et al. Preterm neonatal morbidity and mortality by gestational age: a contemporary cohort. Am J Obstet Gynecol 2016;215:103.e1–14.
   PubMed Web of Science ®Google Scholar
• Romero R, Salafia CM, Athanassiadis AP, et al. The relationship between acute inflammatory lesions of the preterm placenta and amniotic fluid microbiology. Am J Obstet Gynecol 1992;166:1382–8.
   PubMed Web of Science ®Google Scholar
• Hillier SL, Witkin SS, Krohn MA, et al. The relationship of amniotic fluid cytokines and preterm delivery, amniotic fluid infection, histologic chorioamnionitis, and chorioamnion infection. Obstet Gynecol 1993;81:941–48.
   PubMed Web of Science ®Google Scholar
• Odibo AO, Rodis JF, Sanders MM, et al. Relationship of amniotic fluid markers of intra-amniotic infection with histopathology in cases of preterm labor with intact membranes. J Perinatol 1999;19:407–12.
   PubMedGoogle Scholar
• Park CW, Yoon BH, Park JS, Jun JK. An elevated maternal serum C-reactive protein in the context of intra-amniotic inflammation is an indicator that the development of amnionitis, an intense fetal and AF inflammatory response are likely in patients with preterm labor: clinical implications. J Matern Fetal Neonatal Med 2013;26:847–53.
   PubMed Web of Science ®Google Scholar
• Romero R, Miranda J, Chaiworapongsa T, et al. A novel molecular microbiologic technique for the rapid diagnosis of microbial invasion of the amniotic cavity and intra-amniotic infection in preterm labor with intact membranes. Am J Reprod Immunol 2014;71:330–58.
   PubMed Web of Science ®Google Scholar
• Romero R, Miranda J, Chaemsaithong P, et al. Sterile and microbial-associated intra-amniotic inflammation in preterm prelabor rupture of membranes. J Matern Fetal Neonatal Med 2015;28:1394–409.
   PubMed Web of Science ®Google Scholar
• Kim SM, Romero R, Park JW, et al. The relationship between the intensity of intra-amniotic inflammation and the presence and severity of acute histologic chorioamnionitis in preterm gestation. J Matern Fetal Neonatal Med 2015;28:1500–9.
   PubMed Web of Science ®Google Scholar
• Kim CJ, Romero R, Chaemsaithong P, et al. Acute chorioamnionitis and funisitis: definition, pathologic features, and clinical significance. Am J Obstet Gynecol 2015;213:S29–S52.
   PubMed Web of Science ®Google Scholar
• Park CW, Park JS, Moon KC, Jun JK, Yoon BH. Preterm labor and preterm premature rupture of membranes have a different pattern in the involved compartments of acute histologoic chorioamnionitis and/or funisitis: patho-physiologic implication related to different clinical manifestations. Pathol Int 2016;66:325–32.
   PubMed Web of Science ®Google Scholar
• Lee SE, Romero R, Kim CJ, et al. Funisitis in term pregnancy is associated with microbial invasion of the amniotic cavity and intra-amniotic inflammation. J Matern Fetal Neonatal Med 2006;19:693–97.
   PubMed Web of Science ®Google Scholar
• Kacerovsky MVF, Kutova R, Pliskova L, et al. Cervical microbiota in women with preterm prelabor rupture of membranes. PLoS One 2015;10:e0126884.
   PubMed Web of Science ®Google Scholar
• Park CWKS, Park JS, Jun JK, Yoon BH. Fetal, amniotic and maternal inflammatory responses in early stage of ascending intrauterine infection, inflammation restricted to chorio-decidua, in preterm gestation. J Matern Fetal Neonatal Med 2014;27:98–105.
   PubMed Web of Science ®Google Scholar
• Revello RAM, Dudzik D, Abehsera D, Bartha JL. Differential amniotic fluid cytokine profile in women with chorioamnionitis with and without funisitis. J Matern Fetal Neonatal Med 2016;29:2161–65.
   PubMed Web of Science ®Google Scholar
• Romero R, Miranda J, Chaiworapongsa T, et al. Sterile intra-amniotic inflammation in asymptomatic patients with a sonographic short cervix: prevalence and clinical significance. J Matern Fetal Neonatal Med 2014;1–17.
   PubMed Web of Science ®Google Scholar
• Romero R, Miranda J, Kusanovic JP, et al. Clinical chorioamnionitis at term I: microbiology of the amniotic cavity using cultivation and molecular techniques. J Perinat Med 2015;43:19–36.
   PubMed Web of Science ®Google Scholar
• Maymon E, Romero R, Pacora P, et al. Human neutrophil collagenase (matrix metalloproteinase 8) in parturition, premature rupture of the membranes, and intrauterine infection. Am J Obstet Gynecol 2000;183:94–99.
   PubMed Web of Science ®Google Scholar
• Maymon E, Romero R, Chaiworapongsa T, et al. Value of amniotic fluid neutrophil collagenase concentrations in preterm premature rupture of membranes. Am J Obstet Gynecol 2001;185:1143–48.
   PubMed Web of Science ®Google Scholar
• Maymon E, Romero R, Chaiworapongsa T, et al. Amniotic fluid matrix metalloproteinase-8 in preterm labor with intact membranes. Am J Obstet Gynecol 2001;185:1149–55.
   PubMed Web of Science ®Google Scholar
• Yoon BH, Oh SY, Romero R, et al. An elevated amniotic fluid matrix metalloproteinase-8 level at the time of mid-trimester genetic amniocentesis is a risk factor for spontaneous preterm delivery. Am J Obstet Gynecol 2001;185:1162–67.
   PubMed Web of Science ®Google Scholar
• Park JS, Romero R, Yoon BH, et al. The relationship between amniotic fluid matrix metalloproteinase-8 and funisitis. Am J Obstet Gynecol 2001;185:1156–61.
   PubMed Web of Science ®Google Scholar
• Angus SR, Segel SY, Hsu CD, et al. Amniotic fluid matrix metalloproteinase-8 indicates intra-amniotic infection. Am J Obstet Gynecol 2001;185:1232–8.
   PubMed Web of Science ®Google Scholar
• Moon JB, Kim JC, Yoon BH, et al. Amniotic fluid matrix metalloproteinase-8 and the development of cerebral palsy. J Perinat Med 2002;30:301–6.
   PubMed Web of Science ®Google Scholar
• Biggio JR Jr, Ramsey PS, Cliver SP, et al. Midtrimester amniotic fluid matrix metalloproteinase-8 (MMP-8) levels above the 90th percentile are a marker for subsequent preterm premature rupture of membranes. Am J Obstet Gynecol 2005;192:109–13.
   PubMed Web of Science ®Google Scholar
• Nien JK, Yoon BH, Espinoza J, et al. A rapid MMP-8 bedside test for the detection of intra-amniotic inflammation identifies patients at risk for imminent preterm delivery. Am J Obstet Gynecol 2006;195:1025–30.
   PubMed Web of Science ®Google Scholar
• Kim KW, Romero R, Park HS, et al. A rapid matrix metalloproteinase-8 bedside test for the detection of intraamniotic inflammation in women with preterm premature rupture of membranes. Am J Obstet Gynecol 2007;197:292 e1–5.
   PubMed Web of Science ®Google Scholar
• Park CW, Lee SM, Park JS, et al. The antenatal identification of funisitis with a rapid MMP-8 bedside test. J Perinat Med 2008;36:497–502.
   PubMed Web of Science ®Google Scholar
• Lee SJ, Won HS, Kim MN, et al. Diagnostic value of the matrix metalloproteinase-8 rapid test for detecting microbial invasion of the amniotic cavity. Eur J Clin Microbiol Infect Dis 2008;27:1257–60.
   PubMed Web of Science ®Google Scholar
• Kim A, Lee ES, Shin JC, Kim HY. Identification of biomarkers for preterm delivery in mid-trimester amniotic fluid. Placenta 2013;34:873–8.
   PubMed Web of Science ®Google Scholar
• Park HS, Kim SA. Abstract No 322: The value of the genedia MMP-8 rapid test for diagnosing intraamniotic infection/inflammation and predicting adverse pregnancy outcomes in women with preterm premature rupture of membranes. Am J Obstet Gynecol 2015;212:S174.
   PubMed Web of Science ®Google Scholar
• Kim SM, Romero R, Lee J, et al. About one-half of early spontaneous preterm deliveries can be identified by a rapid matrix metalloproteinase-8 (MMP-8) bedside test at the time of mid-trimester genetic amniocentesis. J Matern Fetal Neonatal Med 2016;29:2414–21.
   PubMed Web of Science ®Google Scholar
• Chaemsaithong P, Romero R, Docheva N, et al. A rapid point-of-care test (MMP-8) for the identification of intra-amniotic inflammation and impending preterm delivery. Abstract presented at 12th World Congress of Perinatal Medicine, 3rd–6th November, 2015, Madrid, Spain.
   Google Scholar
• Chaemsaithong P, Romero R, Chaiyasit N, et al. Rapid MMP-8 as a point-of-care test in the identification of intra-amniotic inflammation in patients with preterm PROM. Abstract presented at 12th World Congress of Perinatal Medicine, 3rd–6th November, 2015, Madrid, Spain.
   Google Scholar
• Chaiyasit N, Chaemsaithong P, Romero R, et al. A rapid MMP-8 test for the identification of intra-amniotic inflammation/infection in patients with clinical chorioamnionitis at term: a solution at last! Abstract presented at 12th World Congress of Perinatal Medicine, 3rd-6th November, 2015, Madrid, Spain.
   Google Scholar
• Romero R, Avila C, Santhanam U, Sehgal PB. Amniotic fluid interleukin 6 in preterm labor. Association with infection. J Clin Investig 1990;85:1392–400.
   PubMed Web of Science ®Google Scholar
• Santhanam U, Avila C, Romero R, et al. Cytokines in normal and abnormal parturition: elevated amniotic fluid interleukin-6 levels in women with premature rupture of membranes associated with intrauterine infection. Cytokine 1991;3:155–63.
   PubMed Web of Science ®Google Scholar
• Romero R, Sepulveda W, Kenney JS, et al. Interleukin 6 determination in the detection of microbial invasion of the amniotic cavity. Ciba Found Symp 1992;167:205–20. discussion 20–23.
   PubMedGoogle Scholar
• Romero R, Yoon BH, Mazor M, et al. The diagnostic and prognostic value of amniotic fluid white blood cell count, glucose, interleukin-6, and gram stain in patients with preterm labor and intact membranes. Am J Obstet Gynecol 1993;169:805–16.
   PubMed Web of Science ®Google Scholar
• Romero R, Yoon BH, Mazor M, et al. A comparative study of the diagnostic performance of amniotic fluid glucose, white blood cell count, interleukin-6, and gram stain in the detection of microbial invasion in patients with preterm premature rupture of membranes. Am J Obstet Gynecol 1993;169:839–51.
   PubMed Web of Science ®Google Scholar
• Romero R, Yoon BH, Kenney JS, et al. Amniotic fluid interleukin-6 determinations are of diagnostic and prognostic value in preterm labor. Am J Reprod Immunol 1993;30:167–83.
   PubMed Web of Science ®Google Scholar
• Greig PC, Ernest JM, Teot L, et al. Amniotic fluid interleukin-6 levels correlate with histologic chorioamnionitis and amniotic fluid cultures in patients in premature labor with intact membranes. Am J Obstet Gynecol 1993;169:1035–44.
   PubMed Web of Science ®Google Scholar
• Gomez R, Romero R, Galasso M, et al. The value of amniotic fluid interleukin-6, white blood cell count, and gram stain in the diagnosis of microbial invasion of the amniotic cavity in patients at term. Am J Reprod Immunol 1994;32:200–10.
   PubMed Web of Science ®Google Scholar
• Coultrip LL, Lien JM, Gomez R, et al. The value of amniotic fluid interleukin-6 determination in patients with preterm labor and intact membranes in the detection of microbial invasion of the amniotic cavity. Am J Obstet Gynecol 1994;171:901–11.
   PubMed Web of Science ®Google Scholar
• Dudley DJ, Hunter C, Mitchell MD, Varner MW. Clinical value of amniotic fluid interleukin-6 determinations in the management of preterm labour. Br J Obstet Gynaecol 1994;101:592–97.
   PubMedGoogle Scholar
• Yoon BH, Romero R, Kim CJ, et al. Amniotic fluid interleukin-6: a sensitive test for antenatal diagnosis of acute inflammatory lesions of preterm placenta and prediction of perinatal morbidity. Am J Obstet Gynecol 1995;172:960–70.
   PubMed Web of Science ®Google Scholar
• Negishi H, Yamada H, Mikuni M, et al. Correlation between cytokine levels of amniotic fluid and histological chorioamnionitis in preterm delivery. J Perinat Med 1996;24:633–9.
   PubMed Web of Science ®Google Scholar
• Yoon BH, Jun JK, Romero R, et al. Amniotic fluid inflammatory cytokines (interleukin-6, interleukin-1beta, and tumor necrosis factor-alpha), neonatal brain white matter lesions, and cerebral palsy. Am J Obstet Gynecol 1997;177:19–26.
   PubMed Web of Science ®Google Scholar
• Yoon BH, Romero R, Jun JK, et al. Amniotic fluid cytokines (interleukin-6, tumor necrosis factor-alpha, interleukin-1 beta, and interleukin-8) and the risk for the development of bronchopulmonary dysplasia. Am J Obstet Gynecol 1997;177:825–30.
   PubMed Web of Science ®Google Scholar
• Wenstrom KD, Andrews WW, Hauth JC, et al. Elevated second-trimester amniotic fluid interleukin-6 levels predict preterm delivery. Am J Obstet Gynecol 1998;178:546–50.
   PubMed Web of Science ®Google Scholar
• Baud O, Emilie D, Pelletier E, et al. Amniotic fluid concentrations of interleukin-1beta, interleukin-6 and TNF-alpha in chorioamnionitis before 32 weeks of gestation: histological associations and neonatal outcome. Br J Obstet Gynaecol 1999;106:72–77.
   PubMedGoogle Scholar
• Yoon BH, Park CW, Chaiworapongsa T. Intrauterine infection and the development of cerebral palsy. BJOG 2003;110:124–27.
   PubMed Web of Science ®Google Scholar
• Jacobsson B, Mattsby-Baltzer I, Andersch B, et al. Microbial invasion and cytokine response in amniotic fluid in a Swedish population of women with preterm prelabor rupture of membranes. Acta Obstet Gynecol Scand 2003;82:423–31.
   PubMed Web of Science ®Google Scholar
• Jacobsson B, Mattsby-Baltzer I, Hagberg H. Interleukin-6 and interleukin-8 in cervical and amniotic fluid: relationship to microbial invasion of the chorioamniotic membranes. BJOG 2005;112:719–24.
   PubMed Web of Science ®Google Scholar
• Holst RM, Laurini R, Jacobsson B, et al. Expression of cytokines and chemokines in cervical and amniotic fluid: relationship to histological chorioamnionitis. J Matern Fetal Neonatal Med 2007;20:885–93.
   PubMed Web of Science ®Google Scholar
• Menon R, Camargo MC, Thorsen P, et al. Amniotic fluid interleukin-6 increase is an indicator of spontaneous preterm birth in white but not black Americans. Am J Obstet Gynecol 2008;198:77.e1–7.
   PubMed Web of Science ®Google Scholar
• Cobo T, Palacio M, Navarro-Sastre A, et al. Predictive value of combined amniotic fluid proteomic biomarkers and interleukin-6 in preterm labor with intact membranes. Am J Obstet Gynecol 2009;200:499 e1–6.
   PubMed Web of Science ®Google Scholar
• Massaro G, Scaravilli G, Simeone S, et al. Interleukin-6 and Mycoplasma hominis as markers of preterm birth and related brain damage: our experience. J Matern Fetal Neonatal Med 2009;22:1063–67.
   PubMed Web of Science ®Google Scholar
• Thomakos N, Daskalakis G, Papapanagiotou A, et al. Amniotic fluid interleukin-6 and tumor necrosis factor-alpha at mid-trimester genetic amniocentesis: relationship to intra-amniotic microbial invasion and preterm delivery. Eur J Obstet Gynecol Reproduct Biol 2010;148:147–51.
   PubMed Web of Science ®Google Scholar
• Marconi C, de Andrade Ramos BR, Peracoli JC, et al. Amniotic fluid interleukin-1 beta and interleukin-6, but not interleukin-8 correlate with microbial invasion of the amniotic cavity in preterm labor. Am J Reprod Immunol 2011;65:549–56.
   PubMed Web of Science ®Google Scholar
• Cobo T, Kacerovsky M, Palacio M, et al. A prediction model of histological chorioamnionitis and funisitis in preterm prelabor rupture of membranes: analyses of multiple proteins in the amniotic fluid. J Matern Fetal Neonatal Med 2012;25:1995–2001.
   PubMed Web of Science ®Google Scholar
• Cobo T, Kacerovsky M, Holst RM, et al. Intra-amniotic inflammation predicts microbial invasion of the amniotic cavity but not spontaneous preterm delivery in preterm prelabor membrane rupture. Acta Obstet Gynecol Scand 2012;91:930–35.
   PubMed Web of Science ®Google Scholar
• Bogavac M, Brkic S, Simin N, Celic D. Mid-pregnancy interleukins levels in serum and amniotic fluid as predictors of preterm delivery. J Matern Fetal Neonatal Med 2012. [Epub ahead of print]. doi: 10.3109/14767058.2012.722709
   Google Scholar
• Romero R, Kadar N, Miranda J, et al. The diagnostic performance of the Mass Restricted (MR) score in the identification of microbial invasion of the amniotic cavity or intra-amniotic inflammation is not superior to amniotic fluid interleukin-6. J Matern Fetal Neonatal Med 2014;27:757–69.
   PubMed Web of Science ®Google Scholar
• Kacerovsky M, Musilova I, Hornychova H, et al. Bedside assessment of amniotic fluid interleukin-6 in preterm prelabor rupture of membranes. Am J Obstet Gynecol 2014;211:385 e1–9.
   PubMed Web of Science ®Google Scholar
• Cobo T, Jacobsson B, Kacerovsky M, et al. Systemic and local inflammatory response in women with preterm prelabor rupture of membranes. PloS One 2014;9:e85277.
   PubMed Web of Science ®Google Scholar
• Chaemsaithong P, Romero R, Korzeniewski SJ, et al. A point of care test for the determination of amniotic fluid interleukin-6 and the chemokine CXCL-10/IP-10. J Matern Fetal Neonatal Med 2015;28:1510–19.
   PubMed Web of Science ®Google Scholar
• Kacerovsky M, Musilova I, Stepan M, et al. Detection of intraamniotic inflammation in fresh and processed amniotic fluid samples with the interleukin-6 point of care test. Am J Obstet Gynecol 2015;213:435–36.
   PubMed Web of Science ®Google Scholar
• Chaemsaithong P, Romero R, Korzeniewski SJ, et al. A rapid interleukin-6 bedside test for the identification of intra-amniotic inflammation in preterm labor with intact membranes. J Matern Fetal Neonatal Med 2016;29:349–59.
   PubMed Web of Science ®Google Scholar
• Chaemsaithong P, Romero R, Korzeniewski SJ, et al. A point of care test for interleukin-6 in amniotic fluid in preterm prelabor rupture of membranes: a step toward the early treatment of acute intra-amniotic inflammation/infection. J Matern Fetal Neonatal Med 2016;29:360–67.
   PubMed Web of Science ®Google Scholar
• Sennstrom MB, Brauner A, Bystrom B, et al. Matrix metalloproteinase-8 correlates with the cervical ripening process in humans. Acta Obstet Gynecol Scand 2003;82:904–11.
   PubMed Web of Science ®Google Scholar
• Diaz-Cueto L, Cuica-Flores A, Ziga-Cordero F, et al. Vaginal matrix metalloproteinase levels in pregnant women with bacterial vaginosis. J Soc Gynecol Investig 2006;13:430–34.
   PubMedGoogle Scholar
• Rahkonen L, Rutanen EM, Unkila-Kallio L, et al. Factors affecting matrix metalloproteinase-8 levels in the vaginal and cervical fluids in the first and second trimester of pregnancy. Hum Reprod 2009;24:2693–702.
   PubMed Web of Science ®Google Scholar
• Schefold JC, Hasper D, von Haehling S, et al. Interleukin-6 serum level assessment using a new qualitative point-of-care test in sepsis: a comparison with ELISA measurements. Clin Biochem 2008;41:893–98.
   PubMed Web of Science ®Google Scholar
• Meem M, Modak JK, Mortuza R, et al. Biomarkers for diagnosis of neonatal infections: a systematic analysis of their potential as a point-of-care diagnostics. J Global Health 2011;1:201–9.
   PubMedGoogle Scholar
• Batfalsky A, Lohr A, Heussen N, et al. Diagnostic value of an interleukin-6 bedside test in term and preterm neonates at the time of clinical suspicion of early- and late-onset bacterial infection. Neonatology 2012;102:37–44.
   PubMed Web of Science ®Google Scholar
• Gutierres SL, Welty TE. Point-of-care testing: an introduction. Ann Pharmacother 2004;38:119–25.
   PubMed Web of Science ®Google Scholar
• Kost GJ, Tran NK. Point-of-care testing and cardiac biomarkers: the standard of care and vision for chest pain centers. Cardiol Clin 2005;23:467–90.
   PubMed Web of Science ®Google Scholar
• Lewandrowski K. Point-of-care testing: an overview and a look to the future (circa 2009, United States). Clin Lab Med 2009;29:421–32.
   PubMed Web of Science ®Google Scholar
• Pfafflin A, Schleicher E. Inflammation markers in point-of-care testing (POCT). Anal Bioanal Chem 2009;393:1473–80.
   PubMed Web of Science ®Google Scholar
• Vousden N, Chandiramani M, Seed P, Shennan A. Interleukin-6 bedside testing in women at high risk of preterm birth. J Matern Fetal Neonatal Med 2011;24:1301–4.
   PubMed Web of Science ®Google Scholar
• Berthiaume M, Rousseau E, Rola-Pleszczynski M, Pasquier JC. Rapid evaluation of the absence of inflammation after rupture of membranes. J Matern Fetal Neonatal Med 2014;27:865–69.
   PubMed Web of Science ®Google Scholar
• Romero R, Quintero R, Nores J, et al. Amniotic fluid white blood cell count: a rapid and simple test to diagnose microbial invasion of the amniotic cavity and predict preterm delivery. Am J Obstet Gynecol 1991;165:821–30.
   PubMed Web of Science ®Google Scholar
• Romero R, Jimenez C, Lohda AK, et al. Amniotic fluid glucose concentration: a rapid and simple method for the detection of intraamniotic infection in preterm labor. Am J Obstet Gynecol 1990;163:968–74.
   PubMed Web of Science ®Google Scholar
• Romero R, Emamian M, Quintero R, et al. The value and limitations of the Gram stain examination in the diagnosis of intraamniotic infection. Am J Obstet Gynecol 1988;159:114–9.
   PubMed Web of Science ®Google Scholar
• http://milenia-biotec.de/immunology/mileniar-quickline-il-6/?L=1. 2016.
   Google Scholar
• Galen RS, Gambino SR, Evaluation of laboratory tests: comparing sensitivity and specificity data. Beyond Normality: the Predictive Value and Efficiency of Medical Diagnoses. New York: John Wiley & Sons; 1975. p. 131–40.
   Google Scholar
• Romero R, Kadar N, Hobbins JC, Duff GW. Infection and labor: the detection of endotoxin in amniotic fluid. Am J Obstet Gynecol 1987;157:815–19.
   PubMed Web of Science ®Google Scholar
• Romero R, Roslansky P, Oyarzun E, et al. Labor and infection. II. Bacterial endotoxin in amniotic fluid and its relationship to the onset of preterm labor. Am J Obstet Gynecol 1988;158:1044–49.
   PubMed Web of Science ®Google Scholar
• Romero R, Grivel JC, Tarca AL, et al. Evidence of perturbations of the cytokine network in preterm labor. Am J Obstet Gynecol 2015;213:836 e1–e18.
   PubMed Web of Science ®Google Scholar
• Espinoza J, Chaiworapongsa T, Romero R, et al. Antimicrobial peptides in amniotic fluid: defensins, calprotectin and bacterial/permeability-increasing protein in patients with microbial invasion of the amniotic cavity, intra-amniotic inflammation, preterm labor and premature rupture of membranes. J Matern Fetal Neonatal Med 2003;13:2–21.
   PubMedGoogle Scholar
• Soto E, Espinoza J, Nien JK, et al. Human beta-defensin-2: a natural antimicrobial peptide present in amniotic fluid participates in the host response to microbial invasion of the amniotic cavity. J Matern Fetal Neonatal Med 2007;20:15–22.
   PubMed Web of Science ®Google Scholar
• Owen CA, Hu Z, Lopez-Otin C, Shapiro SD. Membrane-bound matrix metalloproteinase-8 on activated polymorphonuclear cells is a potent, tissue inhibitor of metalloproteinase-resistant collagenase and serpinase. J Immunol 2004;172:7791–803.
   PubMed Web of Science ®Google Scholar
• Dejonckheere E, Vandenbroucke RE, Libert C. Matrix metalloproteinase8 has a central role in inflammatory disorders and cancer progression. Cytokine Growth Factor Rev 2011;22:73–81.
   PubMed Web of Science ®Google Scholar
• Van Lint P, Libert C. Matrix metalloproteinase-8: cleavage can be decisive. Cytokine Growth Factor Rev 2006;17:217–23.
   PubMed Web of Science ®Google Scholar
• Herman MP, Sukhova GK, Libby P, et al. Expression of neutrophil collagenase (matrix metalloproteinase-8) in human atheroma: a novel collagenolytic pathway suggested by transcriptional profiling. Circulation 2001;104:1899–904.
   PubMed Web of Science ®Google Scholar
• Prikk K, Maisi P, Pirila E, et al. In vivo collagenase-2 (MMP-8) expression by human bronchial epithelial cells and monocytes/macrophages in bronchiectasis. J Pathol 2001;194:232–8.
   PubMed Web of Science ®Google Scholar
• Zheng L, Lam WK, Tipoe GL, et al. Overexpression of matrix metalloproteinase-8 and -9 in bronchiectatic airways in vivo. Eur Respir J 2002;20:170–76.
   PubMed Web of Science ®Google Scholar
• Wahlgren J, Maisi P, Sorsa T, et al. Expression and induction of collagenases (MMP-8 and -13) in plasma cells associated with bone-destructive lesions. J Pathol 2001;194:217–24.
   PubMed Web of Science ®Google Scholar
• Toft-Hansen H, Nuttall RK, Edwards DR, Owens T. Key metalloproteinases are expressed by specific cell types in experimental autoimmune encephalomyelitis. J Immunol 2004;173:5209–18.
   PubMed Web of Science ®Google Scholar
• Tervahartiala T, Pirila E, Ceponis A, et al. The in vivo expression of the collagenolytic matrix metalloproteinases (MMP-2, -8, -13, and -14) and matrilysin (MMP-7) in adult and localized juvenile periodontitis. J Den Res 2000;79:1969–77.
   PubMed Web of Science ®Google Scholar
• Pirila E, Ramamurthy N, Maisi P, et al. Wound healing in ovariectomized rats: effects of chemically modified tetracycline (CMT-8) and estrogen on matrix metalloproteinases -8, -13 and type I collagen expression. Curr Med Chem 2001;8:281–94.
   PubMed Web of Science ®Google Scholar
• O'Brien TP, Li QJ, Sauerburger F, et al. The role of matrix metalloproteinases in ulcerative keratolysis associated with perioperative diclofenac use. Ophthalmology 2001;108:656–59.
   PubMed Web of Science ®Google Scholar
• Pirila E, Ramamurthy NS, Sorsa T, et al. (MMP-2), collagenase-2 (MMP-8), and laminin-5 gamma2-chain expression in murine inflammatory bowel disease (ulcerative colitis). Dig Dis Sci 2003;48:93–98.
   PubMed Web of Science ®Google Scholar
• Hanemaaijer R, Sorsa T, Konttinen YT, et al. Matrix metalloproteinase-8 is expressed in rheumatoid synovial fibroblasts and endothelial cells. Regulation by tumor necrosis factor-alpha and doxycycline. J Biol Chem 1997;272:31504–9.
   PubMed Web of Science ®Google Scholar
• Stenman M, Paju A, Hanemaaijer R, et al. Collagenases (MMP-1, -8 and -13) and trypsinogen-2 in fluid from benign and malignant ovarian cysts. Tumour Biol 2003;24:9–12.
   PubMed Web of Science ®Google Scholar
• Knauper V, Kramer S, Reinke H, Tschesche H. Characterization and activation of procollagenase from human polymorphonuclear leucocytes. N-terminal sequence determination of the proenzyme and various proteolytically activated forms. Eur J Biochem/FEBS 1990;189:295–300.
   PubMedGoogle Scholar
• Knauper V, Wilhelm SM, Seperack PK, DeClerck YA, Langley KE, Osthues A, et al. Direct activation of human neutrophil procollagenase by recombinant stromelysin. Biochem J 1993;295:581–86.
   PubMed Web of Science ®Google Scholar
• Balbin M, Fueyo A, Knauper V, et al. Collagenase 2 (MMP-8) expression in murine tissue-remodeling processes. Analysis of its potential role in postpartum involution of the uterus. J Biol Chem 1998;273:23959–68.
   PubMed Web of Science ®Google Scholar
• Knauper V, Murphy G, Tschesche H. Activation of human neutrophil procollagenase by stromelysin 2. Eur J Biochem/FEBS 1996;235:187–91.
   PubMedGoogle Scholar
• Okamoto T, Akaike T, Suga M, et al. Activation of human matrix metalloproteinases by various bacterial proteinases. J Biol Chem 1997;272:6059–66.
   PubMed Web of Science ®Google Scholar
• Schmid TM, Mayne R, Jeffrey JJ, Linsenmayer TF. Type X collagen contains two cleavage sites for a vertebrate collagenase. J Biol Chem 1986;261:4184–89.
   PubMed Web of Science ®Google Scholar
• Hasty KA, Jeffrey JJ, Hibbs MS, Welgus HG. The collagen substrate specificity of human neutrophil collagenase. J Biol Chem 1987;262:10048–52.
   PubMed Web of Science ®Google Scholar
• Pirila E, Sharabi A, Salo T, et al. Matrix metalloproteinases process the laminin-5 gamma 2-chain and regulate epithelial cell migration. Biochem Biophys Res Commun 2003;303:1012–17.
   PubMed Web of Science ®Google Scholar
• Shapiro SD. Matrix metalloproteinase degradation of extracellular matrix: biological consequences. Curr Opin Cell Biol 1998;10:602–8.
   PubMed Web of Science ®Google Scholar
• Van Den Steen PE, Wuyts A, Husson SJ, et al. Gelatinase B/MMP-9 and neutrophil collagenase/MMP-8 process the chemokines human GCP-2/CXCL6, ENA-78/CXCL5 and mouse GCP-2/LIX and modulate their physiological activities. Eur J Biochem/FEBS 2003;270:3739–49.
   PubMedGoogle Scholar
• Van den Steen PE, Husson SJ, Proost P, et al. Carboxyterminal cleavage of the chemokines MIG and IP-10 by gelatinase B and neutrophil collagenase. Biochem Biophys Res Commun 2003;310:889–96.
   PubMed Web of Science ®Google Scholar
• McQuibban GA, Gong JH, Wong JP, et al. Matrix metalloproteinase processing of monocyte chemoattractant proteins generates CC chemokine receptor antagonists with anti-inflammatory properties in vivo. Blood 2002;100:1160–67.
   PubMed Web of Science ®Google Scholar
• Gueders MM, Balbin M, Rocks N, et al. Matrix metalloproteinase-8 deficiency promotes granulocytic allergen-induced airway inflammation. J Immunol 2005;175:2589–97.
   PubMed Web of Science ®Google Scholar
• Kuula H, Salo T, Pirila E, et al. Local and systemic responses in matrix metalloproteinase 8-deficient mice during Porphyromonas gingivalis-induced periodontitis. Infect Immun 2009;77:850–59.
   PubMed Web of Science ®Google Scholar
• Romero R, Gomez R, Mazor M, Ghezzi F, Yoon BH, The preterm labor syndrome. In: Elder MG, Romero R, Lamont RF, editors. Preterm labor. New York: Churchill Livingstone; 1997. 29–49.
   Google Scholar
• Winkler M, Fischer DC, Ruck P, et al. Parturition at term: parallel increases in interleukin-8 and proteinase concentrations and neutrophil count in the lower uterine segment. Hum Reprod 1999;14:1096–100.
   PubMed Web of Science ®Google Scholar
• Horwitz AL, Hance AJ, Crystal RG. Granulocyte collagenase: selective digestion of type I relative to type III collagen. Proc Natl Acad Sci USA 1977;74:897–901.
   PubMed Web of Science ®Google Scholar
• Fortunato SJ, Menon R, Lombardi SJ. MMP/TIMP imbalance in amniotic fluid during PROM: an indirect support for endogenous pathway to membrane rupture. J Perinat Med 1999;27:362–68.
   PubMed Web of Science ®Google Scholar
• Menon R, Fortunato SJ. The role of matrix degrading enzymes and apoptosis in rupture of membranes. J Soc Gynecol Investig 2004;11:427–37.
   PubMedGoogle Scholar
• Weiss A, Goldman S, Shalev E. The matrix metalloproteinases (MMPS) in the decidua and fetal membranes. Front Biosci 2007;12:649–59.
   PubMed Web of Science ®Google Scholar
• Sampson JE, Theve RP, Blatman RN, et al. Fetal origin of amniotic fluid polymorphonuclear leukocytes. Am J Obstet Gynecol 1997;176:77–81.
   PubMed Web of Science ®Google Scholar
• Hunter CA, Jones SA. IL-6 as a keystone cytokine in health and disease. Nat Immunol 2015;16:448–57.
   PubMed Web of Science ®Google Scholar
• Gomez-Lopez N, Olson DM, Robertson SA. Interleukin-6 controls uterine Th9 cells and CD8(+) T regulatory cells to accelerate parturition in mice. Immunol Cell Biol 2016;94:79–89.
   PubMed Web of Science ®Google Scholar