Site-Directed Mutagenesis Reveals Putative Regions of Protein Interaction within the Transmembrane Domains of Connexins

REFERENCES

• Beahm D L, Oshima A, Gaietta G M, Hand G M, Smock A E, Zucker S N, Toloue M M, Chandrasekhar A, Nicholson B J, Sosinsky G E. Mutation of a conserved threonine in the third transmembrane helix of alpha-and beta-connexins creates a dominant-negative closed gap junction channel. J Biol Chem 2006; 281: 7994–8009
   PubMed Web of Science ®Google Scholar
• Braun P, Persson B, Kaback H R, von Heijne G. Alanine insertion scanning mutagenesis of lactose permeasetransmembrane helices. J Biol Chem 1997; 272: 29566–29571
   PubMed Web of Science ®Google Scholar
• Bruzzone R, White T W, Paul D L. Connections with connexins: The molecular basis of direct intercellular signaling. Eur J Biochem 1996; 238: 1–27
   PubMedGoogle Scholar
• Ek J F, Delmar M, Perzova R, Taffet S M. Role of histidine 95 on pH gating of the cardiac gap junction protein connexin43. Circ Res 1994; 74: 1058–1064
   PubMed Web of Science ®Google Scholar
• Fleishman S J, Sabag A D, Ophir E, Avraham K B, Ben-Tal N. The structural context of disease-causing mutations in gap junctions. J Biol Chem 2006; 39: 28958–28963
   Google Scholar
• Fleishman S J, Unger V M, Yeager M, Ben-Tal N. A Cα model for the transmembrane α helices of gap junction intercellular channels. Mol Cell 2004; 15: 878–888
   Google Scholar
• Foote C I, Zhou L, Zhu X, Nicholson B J. The pattern of disulfide linkages in the extracellular loop regions of connexin 32 suggests a model for the docking interface of gap junctions. J Cell Biol 1998; 140: 1187–97
   PubMed Web of Science ®Google Scholar
• Kronengold J, Trexler B, Bukauskas F F, Bargiello T A, Verselis V K. Pore-lining residues identified by single channel SCAM studies in Cx46 hemichannels. Cell Commun Adhes 2003; 10: 193–199
   PubMed Web of Science ®Google Scholar
• Milks L C, Kumar N M, Houghten R, Unwin N, Gilula N B. Topology of the 32-kd liver gap junction protein determined by site-directed antibody localizations. EMBO J 1988; 7: 2967–2975
   PubMed Web of Science ®Google Scholar
• Minor D L, Jr, Masseling S J, Jan Y N, Jan L Y. Transmembrane structure of an inwardly rectifying potassium channel. Cell 1999; 96: 879–891
   PubMed Web of Science ®Google Scholar
• Oh S, Ri Y, Bennett M V, Trexler E B, Verselis V K, Bargiello T A. Changes in permeability caused by connexin 32 mutations underlie X-linked Charcot-Marie-Tooth disease. Neuron 1997; 4: 927–38
   Google Scholar
• Oshima A, Tani K, Hiroaki Y, Fujiyoshi Y, Sosinsky G E. Three-dimensional structure of a human connexin26 gap junction channel reveals a plug in the vestibule. Proc Natl Acad Sci USA 2007; 104: 10034–10039
   PubMed Web of Science ®Google Scholar
• Ri Y, Ballesteros J A, Abrams C K, Oh S, Verselis V K, Weinstein H, Bargiello T A. The role of a conserved proline residue in mediating conformational changes associated with voltage gating of Cx32 gap junctions. Biophys J 1999; 76: 2887–2898
   Google Scholar
• Sharp L L, Zhou J, Blair D F. Tryptophan-scanning mutagenesis of MotB, an integral membrane protein essential for flagellar rotation in Escherichia coli. Biochemistry 1995; 34: 9166–9171
   PubMed Web of Science ®Google Scholar
• Skerrett I M, Smith J F, Nicholson B J. Mechanistic differences between chemical and electrical gating of gap junctions. Current Topics in Membranes, vol. 49, C Peracchia. Academic Press, San Diego, CA 1999; 249–269
   Google Scholar
• Skerrett I M, Merritt M, Zhou L, Zhu H, Cao F-L, Smith J F, Nicholson B J. Applying the Xenopus oocyte expression system to the analysis of gap junction proteins. Methods in Molecular Biology Connexin Methods and Protocols, R Bruzzone, C Giaume. Humana Press, NJ 2000; 225–249
   Google Scholar
• Skerrett I M, Aronowitz J, Shin J H, Cymes G, Kasperek E, Cao F-L, Nicholson B J. Identification of amino acid residues lining the pore of a gap junction channel. J Cell Biol 2002; 159: 349–359
   PubMed Web of Science ®Google Scholar
• Subbiah R N, Kondo M, Campbell T J, Vandenberg J I. Tryptophan scanning mutagenesis of the HERG K+ channel: The S4 domain is loosely packed and likely to be lipid exposed. J Physiol 2005; 569: 367–379
   PubMed Web of Science ®Google Scholar
• Suchyna T M, Xu L X, Gao F, Fourtner C R, Nicholson B J. Identification of a proline residue as a transduction element involved n voltage gating of gap junctions. Nature 1993; 365: 847–849
   PubMed Web of Science ®Google Scholar
• Toloue. Selectivity of Gap Junctions for Natural Metabolites and its Structural Basis. PhD thesis, University of Buffalo, State University of New York, Buffalo, NY 2007
   Google Scholar
• Unger V M, Kumar N M, Gilula N B, Yeager M. Three-dimensional structure of a recombinant gap junction membrane channel. Science 1999; 283: 1176–1180
   PubMed Web of Science ®Google Scholar
• White T W, Bruzzone R, Goodenough D A, Paul D L. Mouse Cx50, a functional member of the connexin family of gap junction proteins, is the lens fiber protein MP70. Mol Biol Cell 1992; 3: 711–720
   PubMed Web of Science ®Google Scholar
• Zhou X W, Pfahnl A, Werner R, Hudder A, Llanes A, Luebke A, Dahl G. Identification of a pore lining segment in gap junction hemichannels. Biophys J 1997; 72: 1946–1953
   PubMed Web of Science ®Google Scholar