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Research Article

Cytotoxic activity and mechanism of action of Smp43 scorpion peptide against colorectal cancer cell line HCT-116

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Received 25 Dec 2023, Accepted 13 Apr 2024, Published online: 23 Apr 2024

References

  • Abdel-Aziz Elzayat, M., et al., 2017. Prevalence of undiagnosed asymptomatic bacteriuria and associated risk factors during pregnancy: a cross-sectional study at two tertiary centres in Cairo, Egypt. BMJ open, 7 (3), e013198.
  • Abdel-Ghani, L.M., et al., 2019. Cytotoxicity of Nubein6.8 peptide isolated from the snake venom of Naja nubiae on melanoma and ovarian carcinoma cell lines. Toxicon: official journal of the international society on toxinology, 168, 22–31.
  • Abdel-Rahman, M. A., Quintero-Hernández, V., and Possani, L. D., 2016. Scorpion venom gland transcriptomics and proteomics: An overview. In: P. Gopalakrishnakone and J. J. Calvete, eds. Venom genomics and proteomics. Dordrecht: Springer Netherlands.
  • Abdel-Rahman, M.A., Quintero-Hernandez, V., and Possani, L.D., 2013. Venom proteomic and venomous glands transcriptomic analysis of the Egyptian scorpion Scorpio maurus palmatus (Arachnida: Scorpionidae). Toxicon, 74, 193–207.
  • Akef, H.M., 2017. Anticancer and antimicrobial activities of scorpion venoms and their peptides. Toxin reviews, 38 (1), 41–53.
  • Almaaytah, A., and Albalas, Q., 2014. Scorpion venom peptides with no disulfide bridges: a review. Peptides, 51, 35–45.
  • Barnum, K. J., and O’connell, M. J., 2014. Cell cycle regulation by checkpoints. In: E. Noguchi and M. C. Gadaleta, eds. Cell cycle control: mechanisms and protocols. New York, NY: Springer New York.
  • Bray, F., et al., 2018. Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA: a Cancer Journal for Clinicians, 68 (6), 394–424.
  • Caliskan, F., et al., 2013. Biological assays on the effects of Acra3 peptide from Turkish scorpion Androctonus crassicauda venom on a mouse brain tumor cell line (BC3H1) and production of specific monoclonal antibodies. Toxicon: official journal of the international society on toxinology, 76, 350–361.
  • Cao, X., and Zhao, B., 2019. Quantitative real-time PCR to measure YAP/TAZ activity in human cells. In: A. Hergovich, ed. The hippo pathway: methods and protocols. New York, NY: Springer New York.
  • Chai, J.W., et al., 2021. Antitumor effects of scorpion peptide Smp43 through mitochondrial dysfunction and membrane disruption on hepatocellular carcinoma. Journal of natural products, 84 (12), 3147–3160.
  • Crowley, L.C., et al., 2016. Quantitation of apoptosis and necrosis by annexin v binding, propidium iodide uptake, and flow cytometry. Cold spring harbor protocols, 2016 (11), 953–957.
  • Cunningham, D., et al., 2010. Colorectal cancer. Lancet (London, England), 375 (9719), 1030–1047.
  • Dai, C.Q., et al., 2016. p53 and mitochondrial dysfunction: novel insight of neurodegenerative diseases. Journal of bioenergetics and biomembranes, 48 (4), 337–347.
  • De Jong, W.H., and Borm, P.J., 2008. Drug delivery and nanoparticles: applications and hazards. International journal of nanomedicine, 3 (2), 133–149.
  • De Rosa, M., et al., 2015. Genetics, diagnosis and management of colorectal cancer (Review). Oncology reports, 34 (3), 1087–1096.
  • Elmore, S., 2007. Apoptosis: a review of programmed cell death. Toxicologic pathology, 35 (4), 495–516.
  • Elrayess, R.A., et al., 2020. Cytotoxic effects of Smp24 and Smp43 scorpion venom antimicrobial peptides on tumour and non-tumour cell lines. International journal of peptide research and therapeutics, 26 (3), 1409–1415.
  • Elrayess, R.A., et al., 2022. Scorpion venom antimicrobial peptides induce caspase-1 dependant pyroptotic cell death. Frontiers in pharmacology, 12, 788874.
  • Fadeyi, S.A., et al., 2013. In vitro anticancer screening of 24 locally used Nigerian medicinal plants. BMC complementary and alternative medicine, 13 (1), 79.
  • Feitelson, M.A., et al., 2015. Sustained proliferation in cancer: mechanisms and novel therapeutic targets. Seminars in cancer biology, 35 Suppl (Suppl), S25–S54.
  • Fink, S.L., and Cookson, B.T., 2005. Apoptosis, pyroptosis, and necrosis: mechanistic description of dead and dying eukaryotic cells. Infection and immunity, 73 (4), 1907–1916.
  • Ford, H.L., Sclafani, R.A., and Degregori, J., 2004. Cell cycle regulatory cascades. Cell cycle and growth control, Second Edition, 95–128.
  • Godefroy, N., et al., 2006. p53 and Retinoblastoma protein (pRb): A complex network of interactions. Apoptosis: an international journal on programmed cell death, 11 (5), 659–661.
  • Guo, R., et al., 2022a. The strong anti-tumor effect of Smp24 in lung adenocarcinoma A549 cells depends on its induction of mitochondrial dysfunctions and ROS accumulation. Toxins, 14 (9), 590.
  • Guo, R.Y., et al., 2022b. Scorpion peptide Smp24 exhibits a potent antitumor effect on human lung cancer cells by damaging the membrane and cytoskeleton in vivo and in vitro. Toxins, 14 (7), 438.
  • Hani, U., et al., 2021. Colorectal cancer: a comprehensive review based on the novel drug delivery systems approach and its management. Journal of drug delivery science and technology, 63, 102532.
  • Haniu, H., et al., 2015. Two-dimensional gel-based protein standardization verified by western blot analysis. In: B. T. Kurien and R. H. Scofield, eds. Western blotting: methods and protocols. New York, NY: Springer New York.
  • Harrison, P.L., et al., 2016. Characterisation of three alpha-helical antimicrobial peptides from the venom of Scorpio maurus palmatus. Toxicon: official journal of the international society on toxinology, 117, 30–36.
  • Heath, G.R., et al., 2018. Visualization of diffusion limited antimicrobial peptide attack on supported lipid membranes. Soft matter, 14 (29), 6146–6154.
  • Hengartner, M.O., 2001. Apoptosis: corralling the corpses. Cell, 104 (3), 325–328.
  • Kerr, D. J., et al., 2016. Oxford textbook of oncology. New York, NY: Oxford University Press.
  • Kilsdonk, M.J., et al., 2014. The impact of organisational external peer review on colorectal cancer treatment and survival in the Netherlands. British journal of cancer, 110 (4), 850–858.
  • Kim, J.H., 2015. Chemotherapy for colorectal cancer in the elderly. World journal of gastroenterology, 21 (17), 5158–5166.
  • Kunda, N.K., 2020. Antimicrobial peptides as novel therapeutics for non-small cell lung cancer. Drug discovery today, 25 (1), 238–247.
  • Le Berre, M., et al., 2022. Calculating half maximal inhibitory concentration (IC50) values from glycomics microarray data using GraphPad prism. In: M. Kilcoyne and J. Q. Gerlach, eds. Glycan microarrays: methods and protocols. New York, NY: Springer US.
  • Li, N., and Zhan, X., 2019. Mitochondrial dysfunction pathway networks and mitochondrial dynamics in the pathogenesis of pituitary adenomas. Frontiers in Endocrinology, 10, 1–18.
  • Montminy, E.M., et al., 2020. Screening for colorectal cancer. The medical clinics of North America, 104 (6), 1023–1036.
  • Nguyen, T., et al., 2022. Smp24, a scorpion-venom peptide, exhibits potent antitumor effects against hepatoma HepG2 Cells via multi-mechanisms in vivo and in vitro. Toxins, 14 (10), 717.
  • O’brien, M.A., and Kirby, R., 2008. Apoptosis: a review of pro-apoptotic and anti-apoptotic pathways and dysregulation in disease. Journal of veterinary emergency and critical care, 18 (6), 572–585.
  • Omran, M.A.A., 2003. Cytotoxic and apoptotic effects of scorpion Leiurus quinquestriatus venom on 293T and C2C12 eukaryotic cell lines. Journal of venomous animals and toxins including tropical diseases, 9 (2), 255–276.
  • Patel, V.A., et al., 2006. Apoptotic cells, at all stages of the death process, trigger characteristic signaling events that are divergent from and dominant over those triggered by necrotic cells: Implications for the delayed clearance model of autoimmunity. The journal of biological chemistry, 281 (8), 4663–4670.
  • Plesca, D., Mazumder, S., and Almasan, A., 2008. DNA damage response and apoptosis. Methods in enzymology, 446, 107–122.
  • Pucci, B., Kasten, M., and Giordano, A., 2000. Cell cycle and apoptosis. Neoplasia (New York, N.Y.), 2 (4), 291–299.
  • Quaglia, A., et al., 2009. The cancer survival gap between elderly and middle-aged patients in Europe is widening. European journal of cancer (Oxford, England: 1990)), 45 (6), 1006–1016.
  • Rahmé, R., 2021. Assaying cell cycle status using flow cytometry. In: J. J. Manfredi, ed. Cell cycle checkpoints: Methods and protocols. New York, NY: Springer US.
  • Rapôso, C., 2017. Scorpion and spider venoms in cancer treatment: state of the art, challenges, and perspectives. Journal of clinical and translational research, 3 (2), 233–249.
  • Rieger, A. M., and Barreda, D. R., 2016. Accurate assessment of cell death by imaging flow cytometry. In: N. S. Barteneva and I. A. Vorobjev, eds. Imaging flow cytometry: methods and protocols. New York, NY: Springer New York.
  • Satyanarayana, A., and Kaldis, P., 2009. Mammalian cell-cycle regulation: several Cdks, numerous cyclins and diverse compensatory mechanisms. Oncogene, 28 (33), 2925–2939.
  • Schwartz, G.K., and Shah, M.A., 2005. Targeting the cell cycle: a new approach to cancer therapy. Journal of clinical oncology: official journal of the american society of clinical oncology, 23 (36), 9408–9421.
  • Schweizer, F., 2009. Cationic amphiphilic peptides with cancer-selective toxicity. European journal of pharmacology, 625 (1-3), 190–194.
  • Selvam, C., et al., 2019. Molecular mechanisms of curcumin and its analogs in colon cancer prevention and treatment. Life sciences, 239, 117032.
  • Sherr, C.J., and Mccormick, F., 2002. The RB and p53 pathways in cancer. Cancer cell, 2 (2), 103–112.
  • Shubin, A.V., et al., 2016. Cytoplasmic vacuolization in cell death and survival. Oncotarget, 7 (34), 55863–55889.
  • Sung, H., et al., 2021. Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA: a cancer journal for clinicians, 71 (3), 209–249.
  • Tan, H. T., et al., 2011. iTRAQ™ labeling coupled with LC-MALDI Mass spectrometry for monitoring temporal response of colorectal cancer cells to butyrate treatment. In: S. D. Satyanarayanajois, ed. Drug design and discovery: methods and protocols. Totowa, NJ: Humana Press.
  • Teleb, W.K., et al., 2022. Cytotoxicity and molecular alterations induced by scorpion venom antimicrobial peptide smp43 in breast cancer cell lines MDA-MB-231 and MCF-7. International journal of peptide research and therapeutics, 29 (1), 8.
  • Tornesello, A.L., et al., 2020. Antimicrobial peptides as anticancer agents: functional properties and biological activities. Molecules (Basel, Switzerland), 25 (12), 2850.
  • Tsai, C.H., et al., 2006. Mechanisms of cardiotoxin lll-induced apoptosis in human colorectal cancer colo205 cells. Clinical and experimental pharmacology & physiology, 33 (3), 177–182.
  • Van Meerloo, J., Kaspers, G. J. L., and Cloos, J., 2011. Cell sensitivity assays: the MTT assay. In: I. A. Cree, ed. Cancer cell culture: methods and protocols. Totowa, NJ: Humana Press.
  • Wolpin, B.M., and Mayer, R.J., 2008. Systemic treatment of colorectal cancer. Gastroenterology, 134 (5), 1296–1310.e1.
  • Wolpin, B.M., et al., 2007. Adjuvant treatment of colorectal cancer. CA: a cancer journal for clinicians, 57 (3), 168–185.
  • Wong, R.S., 2011. Apoptosis in cancer: from pathogenesis to treatment. Journal of experimental & clinical cancer research, 30 (1), 87.
  • Xi, Y., and Xu, P., 2021. Global colorectal cancer burden in 2020 and projections to 2040. Translational oncology, 14 (10), 101174.
  • Ziegler, U., and Groscurth, P., 2004. Morphological features of cell death. News in physiological sciences: an international journal of physiology produced jointly by the international union of physiological sciences and the American physiological society, 19, 124–128.

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