Novel techniques to prevent apoptosis and improve regeneration in corneal endothelial cells

References

• Nishida T, Saika S. Cornea and sclera: anatomy and physiology. In: Krachmer JH, Mannis MJ, Holland EJ, editors. Cornea. Vol. 1. 3rd ed. St. Louis: Mosby; 2011. p. 3–24.
   Google Scholar
• Hassell JR, Birk DE. The molecular basis of corneal transparency. Exp Eye Res. 2010;91(3):326–335.
   PubMed Web of Science ®Google Scholar
• Joyce NC. Proliferative capacity of the corneal endothelium. Prog Retin Eye Res. 2003;22:359–389.
   PubMed Web of Science ®Google Scholar
• Nagata S. Apoptosis by death factor. Cell. 1997;88:355–365.
   PubMed Web of Science ®Google Scholar
• Strasser A, Jost PJ, Nagata S. The many roles of FAS receptor signaling in the immune system. Immunity. 2009;30:180–192.
   PubMed Web of Science ®Google Scholar
• Li H, Zhu H, Xu CJ, et al. Cleavage of BID by caspase 8 mediates the mitochondrial damage in the Fas pathway of apoptosis. Cell. 1998;94(4):491–501.
   PubMed Web of Science ®Google Scholar
• Nagata S. Apoptosis and clearance of apoptotic cells. Annu Rev Immunol. 2018;36:489–517.
   PubMed Web of Science ®Google Scholar
• Gibson CJ, Davids MS. BCL-2 antagonism to target the intrinsic mitochondrial pathway of apoptosis. Clin Cancer Res. 2015;21(22):5021–5029.
   PubMed Web of Science ®Google Scholar
• Jurkunas UV, Bitar MS, Funaki T, et al. Evidence of oxidative stress in the pathogenesis of Fuchs endothelial corneal dystrophy. Am J Pathol. 2010;177:2278–2289.
   PubMed Web of Science ®Google Scholar
• Azizi B, Ziaei A, Fuchsluger T, et al. p53-regulated increase in oxidative-stress–induced apoptosis in Fuchs endothelial corneal dystrophy: a native tissue model. Invest Ophthalmol Vis Sci. 2011;52:9291–9297.
   PubMed Web of Science ®Google Scholar
• Nita M, Grzybowski A. The role of the reactive oxygen species and oxidative stress in the pathomechanism of the age-related ocular diseases and other pathologies of the anterior and posterior eye segments in adults. Oxid Med Cell Longev. 2016;2016:3164734.
   PubMed Web of Science ®Google Scholar
• Buddi R, Lin B, Atilano SR, et al. Evidence of oxidative stress in human corneal diseases. J Histochem Cytochem. 2002;50:341–351.
   PubMed Web of Science ®Google Scholar
• Jurkunas UV, Rawe I, Bitar MS, et al. Decreased expression of peroxiredoxins in Fuchs’ endothelial dystrophy. Invest Ophthalmol Vis Sci. 2008;49:2956–2963.
   PubMed Web of Science ®Google Scholar
• Gottsch JD, Bowers AL, Margulies EH, et al. Serial analysis of gene expression in the corneal endothelium of Fuchs’ dystrophy. Invest Ophthalmol Vis Sci. 2003;44:594–599.
   PubMed Web of Science ®Google Scholar
• Choi WS, Koh JW, Chung TY, et al. Cytotoxicity of ganciclovir on cultured human corneal endothelial cells. Antivir Ther. 2013;18(6):813–820.
   PubMed Web of Science ®Google Scholar
• Hara S, Tsujikawa M, Maruyama K, et al. STAT3 signaling maintains homeostasis through a barrier function and cell survival in corneal endothelial cells. Exp Eye Res. 2019;179:132–141.
   PubMed Web of Science ®Google Scholar
• Yagi-Yaguchi Y, Yamaguchi T, Higa K, et al. Preoperative aqueous cytokine levels are associated with a rapid reduction in endothelial cells after penetrating keratoplasty. Am J Ophthalmol. 2017;181:166–173.
   PubMed Web of Science ®Google Scholar
• Yazu H, Yamaguchi T, Aketa N, et al. Preoperative aqueous cytokine levels are associated with endothelial cell loss after descemet’s stripping automated endothelial keratoplasty. Invest Ophthalmol Vis Sci. 2018;59:612–620.
   PubMed Web of Science ®Google Scholar
• Ayaki M, Iwasawa A, Niwano Y. In vitro assessment of the cytotoxicity of six antibiotic antibiotics to four cultured ocular surface cell lines. Biocontrol Sci. 2012;17(2):93–99.
   PubMed Web of Science ®Google Scholar
• Ayaki M, Taguchi Y, Soda M, et al. Cytotoxicity of antibiotic medications used for infection and inflammation control after cataract surgery in cultured corneal endothelial cells. Biocontrol Sci. 2010;15(3):97–102.
   PubMed Web of Science ®Google Scholar
• Ayaki M, Yaguchi S, Iwasawa A, et al. Cytotoxicity of ophthalmic solutions with and without preservatives to human corneal endothelial cells, epithelial cells and conjunctival epithelial cells. Clin Exp Ophthalmol. 2008;36(6):553–559.
   PubMed Web of Science ®Google Scholar
• Datta S, Baudouin C, Brignole-Baudouin F, et al. The eye drop preservative benzalkonium chloride potently induces mitochondrial dysfunction and preferentially affects LHON mutant cells. Invest Ophthalmol Vis Sci. 2017;58(4):2406–2412.
   PubMed Web of Science ®Google Scholar
• Fan TJ, Wu SX, Jiang GJ. Apoptotic effects of norfloxacin on corneal endothelial cells. Naunyn Schmiedebergs Arch Pharmacol. 2020;393(1):77–88.
   PubMed Web of Science ®Google Scholar
• Gasset AR, Ishii Y, Kaufman HE, et al. Cytotoxicity of ophthalmic preservatives. Am J Ophthalmol. 1974;78(1):98–105.
   PubMed Web of Science ®Google Scholar
• Han SB, Shin YJ, Hyon JY, et al. Cytotoxicity of voriconazole on cultured human corneal endothelial cells. Antimicrob Agents Chemother. 2011;55(10):4519–4523.
   PubMed Web of Science ®Google Scholar
• Li D, Zhang P. Protective effect and molecular mechanism of liquiritin on oxybuprocaine-induced apoptosis of human corneal endothelial cells. Exp Ther Med. 2018;15(4):3432–3438.
   PubMed Web of Science ®Google Scholar
• Li YH, Wen Q, Fan TJ, et al. Dose dependent cytotoxicity of pranoprofen in cultured human corneal endothelial cells by inducing apoptosis. Drug Chem Toxicol. 2015;38(1):16–21.
   PubMed Web of Science ®Google Scholar
• Wen Q, Fan T, Bai S, et al. Cytotoxicity of proparacaine to human corneal endothelial cells in vitro. J Toxicol Sci. 2015;40(4):427–436.
   PubMed Web of Science ®Google Scholar
• Wen Q, Fan TJ, Tian CL. Cytotoxicity of atropine to human corneal endothelial cells by inducing mitochondrion-dependent apoptosis. Exp Biol Med (Maywood). 2016;241(13):1457–1465.
   PubMed Web of Science ®Google Scholar
• Yu HZ, Li YH, Wang RX, et al. Cytotoxicity of lidocaine to human corneal endothelial cells in vitro. Basic Clin Pharmacol Toxicol. 2014;114(4):352–359.
   PubMed Web of Science ®Google Scholar
• Cullen AP. Photokeratitis and other phototoxic effects on the cornea and conjunctiva. Int J Toxicol. 2002;21(6):455–464.
   PubMed Web of Science ®Google Scholar
• Ivanov IV, Mappes T, Schaupp P, et al. Ultraviolet radiation oxidative stress affects eye health. J Biophotonics. 2018;11(7):e201700377.
   PubMed Web of Science ®Google Scholar
• Oliva MS, Taylor H. Ultraviolet radiation and the eye. Int Ophthalmol Clin. 2005;45(1):1–17.
   PubMedGoogle Scholar
• Pastor-Valero M, Fletcher AE, de Stavola BL, et al. Years of sunlight exposure and cataract: a case-control study in a Mediterranean population. BMC Ophthalmol. 2007;7(1):18.
   PubMedGoogle Scholar
• Taylor HR, West SK, Rosenthal FS, et al. Corneal changes associated with chronic UV irradiation. Arch Ophthalmol. 1989;107(10):1481–1484.
   PubMedGoogle Scholar
• Young RW. The family of sunlight-related eye diseases. Optom Vis Sci. 1994;71(2):125–144.
   PubMed Web of Science ®Google Scholar
• Zinfloua C, Rochettea PJ. Ultraviolet A-induced oxidation in cornea: characterization of the early oxidation-related events. Free Radic Biol Med. 2017;108:118–128.
   PubMed Web of Science ®Google Scholar
• Handy DE, Loscalzo J. Redox regulation of mitochondrial function. Antioxid Redox Signal. 2012;16(11):1323–1367.
   PubMed Web of Science ®Google Scholar
• Liu C, Vojnovic D, Kochevar IE, et al. UV-A irradiation activates Nrf2-regulated antioxidant defense and induces p53/caspase3-dependent apoptosis in corneal endothelial cells. Invest Ophthalmol Vis Sci. 2016;57(4):2319–2327.
   PubMed Web of Science ®Google Scholar
• Melles GR, Lander F, Rietveld FJ. Transplantation of Descemet’s membrane carrying viable endothelium through a small scleral incision. Cornea. 2002;21:415–418.
   PubMed Web of Science ®Google Scholar
• Terry MA, Ousley PJ. Deep lamellar endothelial keratoplasty in the first United States patients: early clinical results. Cornea. 2001;20:239–243.
   PubMed Web of Science ®Google Scholar
• Gorovoy MS. Descemet-stripping automated endothelial keratoplasty. Cornea. 2006;25:886–889.
   PubMed Web of Science ®Google Scholar
• Ham L, Dapena I, van Luijk C, et al. Descemet membrane endothelial keratoplasty (DMEK) for Fuchs endothelial dystrophy: review of the first 50 consecutive cases. Eye (Lond). 2009;23:1990–1998.
   PubMed Web of Science ®Google Scholar
• Borkar DS, Veldman P, Colby KA. Treatment of Fuchs endothelial dystrophy by descemet stripping without endothelial keratoplasty. Cornea. 2016;35:1267–1273.
   PubMedGoogle Scholar
• Mathews PM, Lindsley K, Aldave AJ, et al. Etiology of global corneal blindness and current practices of corneal transplantation: a focused review. Cornea. 2018;37:1198–1203.
   PubMed Web of Science ®Google Scholar
• Noritake K, Aki T, Funakoshi T, et al. Critical roles of Rho-associated kinase in membrane blebbing and mitochondrial pathway of apoptosis caused by 1-butanol. Toxicol In Vitro. 2012;26(6):849–855.
   PubMed Web of Science ®Google Scholar
• Croft DR, Coleman ML, Li S, et al. Actin-myosin-based contraction is responsible for apoptotic nuclear disintegration. J Cell Biol. 2005;168:245–255.
   PubMed Web of Science ®Google Scholar
• Krishnan A, Kocab AJ, Zacks DN, et al. small peptide antagonist of the Fas receptor inhibits neuroinflammation and prevents axon degeneration and retinal ganglion cell death in an inducible mouse model of glaucoma. J Neuroinflammation. 2019;16(1):184.
   PubMed Web of Science ®Google Scholar
• Xiao J, Yao J, Jia L, et al. Protective effect of Met12, a small peptide inhibitor of Fas, on the retinal pigment epithelium and photoreceptor after sodium iodate injury effect of Met12 on RPE and photoreceptor after NaIO3 injury. Invest Ophthalmol Vis Sci. 2017;58:1801–1810.
   PubMed Web of Science ®Google Scholar
• King MD, Alleyne CH Jr, Dhandapani KM. TNF-alpha receptor antagonist, R-7050, improves neurological outcomes following intracerebral hemorrhage in mice. Neurosci Lett. 2013;542:92–96.
   PubMed Web of Science ®Google Scholar
• Micheau O. Regulation of TNF-related apoptosis-inducing ligand signaling by glycosylation. Int J Mol Sci. 2018;19(3):pii: E715.
   PubMed Web of Science ®Google Scholar
• Yin XH, Yan JZ, Yang G, et al. PDZ1 inhibitor peptide protects neurons against ischemia via inhibiting GluK2-PSD-95-module-mediated Fas signaling pathway. Brain Res. 2016;1637:64–70.
   PubMed Web of Science ®Google Scholar
• Ray PD, Huang BW, Tsuji Y. Reactive oxygen species (ROS) homeostasis and redox regulation in cellular signaling. Cell Signal. 2012;24(5):981–990.
   PubMed Web of Science ®Google Scholar
• Mittal M, Siddiqui MR, Tran K, et al. Reactive oxygen species in inflammation and tissue injury. Antioxid Redox Signal. 2014;20(7):1126–1167.
   PubMed Web of Science ®Google Scholar
• Ludin A, Gur-Cohen S, Golan K, et al. Reactive oxygen species regulate hematopoietic stem cell self-renewal, migration and development, as well as their bone marrow microenvironment. Antioxid Redox Signal. 2014;21(11):1605–1619.
   PubMed Web of Science ®Google Scholar
• Redza-Dutordoir M, Averill-Bates DA. Activation of apoptosis signaling pathways by reactive oxygen species. Biochim Biophys Acta. 2016;1863(12):2977–2992.
   PubMed Web of Science ®Google Scholar
• Davalli P, Mitic T, Caporali A, et al. ROS, cell senescence, and novel molecular mechanisms in aging and age-related diseases. Oxid Med Cell Longev. 2016;2016:3565127.
   PubMed Web of Science ®Google Scholar
• Zhang Y, Su SS, Zhao S, et al. RIP1 autophosphorylation is promoted by mitochondrial ROS and is essential for RIP3 recruitment into necrosome. Nat Commun. 2017;8:14329.
   PubMed Web of Science ®Google Scholar
• Yang Y, Bazhin AV, Werner J. Reactive oxygen species in the immune system. Int Rev Immunol. 2013;32(3):249–270.
   PubMed Web of Science ®Google Scholar
• Ziaei A, Schmedt T, Chen Y, et al. Sulforaphane decreases endothelial cell apoptosis in fuchs endothelial corneal dystrophy: a novel treatment. Invest Ophthalmol Vis Sci. 2013;54(10):6724–6734.
   PubMed Web of Science ®Google Scholar
• Zernii EY, Gancharova OS, Baksheeva VE, et al. Mitochondria-targeted antioxidant SkQ1 prevents anesthesia induced dry eye syndrome. OxidMed Cell Longevity. 2017;2017:9281519.
   PubMed Web of Science ®Google Scholar
• Zernii EY, Gancharova OS, Tiulina VV, et al. Mitochondria-targeted antioxidant SKQ1 protects cornea from oxidative damage induced by ultraviolet irradiation and mechanical injury. BMC Ophthalmol. 2018;18(1):336.
   PubMedGoogle Scholar
• Petrov A, Perekhvatova N, Skulachev M, et al. SkQ1 ophthalmic solution for dry eye treatment: results of a phase 2 safety and efficacy clinical study in the environment and during challenge in the controlled adverse environment model. Adv Ther. 2016;33(1):96–115.
   PubMed Web of Science ®Google Scholar
• Mancuso C, Santangelo R. Ferulic acid: pharmacological and toxicological aspects. Food Chem Toxicol. 2014;65:185–195.
   PubMed Web of Science ®Google Scholar
• Jin Y, Fan Y, Yan EZ, et al. Effects of sodium ferulate on amyloid-beta-induced MKK3/MKK6-p38 MAPK-Hsp27 signal pathway and apoptosis in rat hippocampus. Acta Pharmacol Sin. 2006;27(10):1309–1316.
   PubMed Web of Science ®Google Scholar
• Kim HY, Lee SM. Ferulic acid attenuates ischemia/reperfusion-induced hepatocyte apoptosis via inhibition of JNK activation. Eur J Pharm Sci. 2012;45(5):708–715.
   PubMed Web of Science ®Google Scholar
• Pei X, Wang W, Miao N, et al. The protective effects of the combination of sodium ferulate and oxymatrine on ethanol induced liver damage in mice. Environ Toxicol Pharm. 2014;37(1):423–430.
   PubMed Web of Science ®Google Scholar
• Qin J, Shang L, Ping AS, et al. TNF/TNFR signal transduction pathway-mediated anti-apoptosis and anti-inflammatory effects of sodium ferulate on IL-1β-induced rat osteoarthritis chondrocytes in vitro. Arthritis Res Ther. 2012;14(6):R242.
   PubMed Web of Science ®Google Scholar
• Tian L, Dang XQ, Wang CS, et al. Effects of sodium ferulate on preventing steroid-induced femoral head osteonecrosis in rabbits. J Zhejiang Univ-Sc B. 2013;14(5):426–437.
   PubMed Web of Science ®Google Scholar
• Jiang G, Fan T. Sodium ferulate attenuates lidocaine-induced corneal endothelial impairment. Oxid Med Cell Longev. 2018;2018:4967318.
   PubMed Web of Science ®Google Scholar
• Gyrd-Hansen M, Meier P. IAPs: from caspase inhibitors to modulators of NF-κB, inflammation and cancer. Nat Rev Cancer. 2010;10(8):561–574.
   PubMed Web of Science ®Google Scholar
• Imre G, Berthelet J, Heering, et al. Apoptosis inhibitor 5 is an endogenous inhibitor of caspase-2. EMBO Rep. 2017;18(5):733–744.
   PubMed Web of Science ®Google Scholar
• Wikström K, Juhas M, Sjölander A. The anti-apoptotic effect of leukotriene D4 involves the prevention of caspase 8 activation and Bid cleavage. Biochem J. 2003 Apr 1;371(Pt 1):115–124.
   PubMedGoogle Scholar
• Wang Z, Watt W, Brooks NA, et al. Kinetic and structural characterization of caspase-3 and caspase-8 inhibition by a novel class of irreversible inhibitors. Biochim Biophys Acta. 2010;1804(9):1817–1831.
   PubMed Web of Science ®Google Scholar
• Coleman ML, Sahai EA, Yeo M, et al. Membrane blebbing during apoptosis results from caspase-mediated activation of ROCK I. Nat Cell Biol. 2001;3(4):339–345.
   PubMed Web of Science ®Google Scholar
• Chang J, Xie M, Shah VR, et al. Activation of Rho-associated coiled-coil protein kinase 1 (ROCK-1) by caspase-3 cleavage plays an essential role in cardiac myocyte apoptosis. Proc Natl Acad Sci U S A. 2006;103(39):14495–14500.
   PubMed Web of Science ®Google Scholar
• Julian L, Olson MF. Rho-associated coiled-coil containing kinases (ROCK): structure, regulation, and functions. Small GTPases. 2014;5:e29846.
   PubMedGoogle Scholar
• Sebbagh M, Renvoizé C, Hamelin J, et al. Caspase-3-mediated cleavage of ROCK I induces MLC phosphorylation and apoptotic membrane blebbing. Nat Cell Biol. 2001;3(4):346–352.
   PubMed Web of Science ®Google Scholar
• Okumura N, Fujii K, Kagami T, et al. Activation of the Rho/Rho Kinase Signaling Pathway Is Involved in Cell Death of Corneal Endothelium. Invest Ophthalmol Vis Sci. 2016;57(15):6843–6851.
   PubMed Web of Science ®Google Scholar
• Hollanders K, Hove IV, Sergeys J, et al. AMA0428, a potent rock inhibitor, attenuates early and late experimental diabetic retinopathy. Curr Eye Res. 2017;42(2):260–272.
   PubMed Web of Science ®Google Scholar
• Macsai MS, Shiloach M. Use of topical Rho kinase inhibitors in the treatment of fuchs dystrophy after descemet stripping only. Cornea. 2019;38(5):529–534.
   PubMed Web of Science ®Google Scholar
• Meekins LC, Rosado-Adames N, Maddala R, et al. Corneal endothelial cell migration and proliferation enhanced by Rho kinase (ROCK) inhibitors in in vitro and in vivo models. Invest Ophthalmol Vis Sci. 2016;57(15):6731–6738.
   PubMed Web of Science ®Google Scholar
• Moura-Coelho N, Tavares Ferreira J, Bruxelas CP, et al. Rho kinase inhibitors-a review on the physiology and clinical use in ophthalmology. Graefes Arch Clin Exp Ophthalmol. 2019;257(6):1101–1117.
   PubMed Web of Science ®Google Scholar
• Okumura N, Koizumi N, Ueno M, et al. The new therapeutic concept of using a rho kinase inhibitor for the treatment of corneal endothelial dysfunction. Cornea. 2011;30(Suppl1):S54–59.
   PubMedGoogle Scholar
• Zeng P, Pi RB, Li P, et al. Fasudil hydrochloride, a potent ROCK inhibitor, inhibits corneal neovascularization after alkali burns in mice. Mol Vis. 2015;21:688–698.
   PubMed Web of Science ®Google Scholar
• Goldstein AS, Aldrich BT, Skeie JM, et al. Assessing the effects of ripasudil, a novel Rho kinase inhibitor, on human corneal endothelial cell health. J Ocul Pharmacol Ther. 2018;34(10):692–699.
   Web of Science ®Google Scholar
• Achiron A, Feldman A, Karmona L, et al. Prophylactic exposure of human corneal endothelial cells to Rho-associated kinase inhibitor reduced apoptosis rate after phacoemulsification: ex vivo study. J Cataract Refract Surg. 2018;44(10):1261–1266.
   PubMed Web of Science ®Google Scholar
• Wang J, Liu XH, Yang ZJ, et al. The effect of ROCK-1 activity change on the adhesive and invasive ability of Y79 retinoblastoma cells. BMC Cancer. 2014;14:89.
   PubMed Web of Science ®Google Scholar
• Wang RF, Williamson JE, Kopczynski C, et al. Effect of 0.04% AR-13324, a ROCK, and norepinephrine transporter inhibitor, on aqueous humor dynamics in normotensive monkey eyes. J Glaucoma. 2015;24(1):51–54.
   PubMed Web of Science ®Google Scholar
• Radell JE, Serle JB. Netarsudil/latanoprost fixed-dose combination for the treatment of open-angle glaucoma or ocular hypertension. Drugs Today (Barc). 2019;55(9):563–574. PubMed PMID: 31584573.
   PubMed Web of Science ®Google Scholar
• Fang Y, Braley-Mullen H. Cultured murine thyroid epithelial cells expressing transgenic Fas-associated death domain-like interleukin-1beta converting enzyme inhibitory protein are protected from fas-mediated apoptosis. Endocrinology. 2008;149(7):3321–3329.
   PubMed Web of Science ®Google Scholar
• Humphreys L, Espona-Fiedler M, Longley DB. FLIP as a therapeutic target in cancer. Febs J. 2018;285(22):4104–4123.
   PubMed Web of Science ®Google Scholar
• Masuko-Hongo K, Sakata M, Yuan GH, et al. Expression of Fas-associated death domain-like interleukin-1beta-converting enzyme (FLICE) inhibitory protein (FLIP) in human articular chondrocytes: possible contribution to the resistance to Fas-mediated death of in vitro cultured human articular chondrocytes. Rheumatol Int. 2001;21(3):112–121.
   PubMed Web of Science ®Google Scholar
• Choi CY, Reimers K, Allmeling C, et al. Inhibition of apoptosis by expression of antiapoptotic proteins in recombinant human keratinocytes. Cell Transplant. 2007;16(6):663–674.
   Web of Science ®Google Scholar
• Wilkie-Grantham RP, Matsuzawa S, Reed JC. Novel phosphorylation and ubiquitination sites regulate reactive oxygen species-dependent degradation of anti-apoptotic c-FLIP protein. J Biol Chem. 2013;288(18):12777–12790.
   PubMed Web of Science ®Google Scholar
• Barcia RN, Dana MR, Kazlauskas A. Corneal graft rejection is accompanied by apoptosis of the endothelium and is prevented by gene therapy with bcl-xL. Am J Transplant. 2007;7(9):2082–2089.
   PubMed Web of Science ®Google Scholar
• Fuchsluger TA, Jurkunas U, Kazlauskas A, et al. Anti-apoptotic gene therapy prolongs survival of corneal endothelial cells during storage. Gene Ther. 2011;18(8):778–787.
   PubMed Web of Science ®Google Scholar
• Fuchsluger TA, Jurkunas U, Kazlauskas A, et al. Corneal endothelial cells are protected from apoptosis by gene therapy. Hum Gene Ther. 2011;22(5):549–558.
   PubMed Web of Science ®Google Scholar
• Fouad AA, Hafez HM, Hamouda A. Hydrogen sulfide modulates IL-6/STAT3 pathway and inhibits oxidative stress, inflammation, and apoptosis in rat model of methotrexate hepatotoxicity. Hum Exp Toxicol. 2019;22:960327119877437.
   Google Scholar
• Dai F, Gao L, Zhao Y, et al. Farrerol inhibited angiogenesis through Akt/mTOR, Erk and Jak2/Stat3 signal pathway. Phytomedicine. 2016;23(7):686–693.
   PubMed Web of Science ®Google Scholar
• Kernt M, Hirneiss C, Neubauer AS, et al. Minocycline is cytoprotective in human corneal endothelial cells and induces anti-apoptotic B-cell CLL/lymphoma 2 (Bcl-2) and X-linked inhibitor of apoptosis (XIAP). Br J Ophthalmol. 2010;94(7):940–946.
   PubMed Web of Science ®Google Scholar