Enzyme-Linked Lipid Nanocarriers for Coping Pseudomonal Pulmonary Infection. Would Nanocarriers Complement Biofilm Disruption or Pave Its Road?

References

• Malhotra S, Hayes D, Wozniak Daniel J. Cystic fibrosis and pseudomonas aeruginosa: the host-microbe interface. Clin Microbiol Rev. 2019;32(3):e00138–00118. doi:10.1128/CMR.00138-18
   PubMed Web of Science ®Google Scholar
• Flemming H-C, Wingender J. The biofilm matrix. Nat Rev Microbiol. 2010;8(9):623–633. doi:10.1038/nrmicro2415
   PubMed Web of Science ®Google Scholar
• Hadinoto K, Cheow WS. Nano-antibiotics in chronic lung infection therapy against Pseudomonas aeruginosa. Colloids Surf B. 2014;116:772–785. doi:10.1016/j.colsurfb.2014.02.032
   PubMed Web of Science ®Google Scholar
• Maunders E, Welch M. Matrix exopolysaccharides; the sticky side of biofilm formation. FEMS Microbiol Lett. 2017;364(13). doi:10.1093/femsle/fnx120
   PubMed Web of Science ®Google Scholar
• Cotton LA, Graham RJ, Lee RJ. The role of alginate in P. aeruginosa PAO1 biofilm structural resistance to gentamicin and ciprofloxacin. J Exp Microbiol Immunol. 2009;13:58–62.
   Google Scholar
• Kim HS, Lee C-G, Lee EY. Alginate lyase: structure, property, and application. Biotechnol Bioprocess Eng. 2011;16(5):843. doi:10.1007/s12257-011-0352-8
   Web of Science ®Google Scholar
• Omri A, Beaulac C, Bouhajib M, Montplaisir S, Sharkawi M, Lagace J. Pulmonary retention of free and liposome-encapsulated tobramycin after intratracheal administration in uninfected rats and rats infected with Pseudomonas aeruginosa. Antimicrob Agents Chemother. 1994;38(5):1090–1095. doi:10.1128/AAC.38.5.1090
   PubMed Web of Science ®Google Scholar
• Patel KK, Tripathi M, Pandey N, et al. Alginate lyase immobilized chitosan nanoparticles of ciprofloxacin for the improved antimicrobial activity against the biofilm associated mucoid P. aeruginosa infection in cystic fibrosis. Int J Pharm. 2019;563:30–42. doi:10.1016/j.ijpharm.2019.03.051
   PubMed Web of Science ®Google Scholar
• Li S, Wang Y, Li X, Lee BS, Jung S, Lee M-S. Enhancing the thermo-stability and anti-biofilm activity of alginate lyase by immobilization on low molecular weight chitosan nanoparticles. Int J Mol Sci. 2019;20(18):4565. doi:10.3390/ijms20184565
   PubMed Web of Science ®Google Scholar
• Günday Türeli N, Torge A, Juntke J, et al. Ciprofloxacin-loaded PLGA nanoparticles against cystic fibrosis P. aeruginosa lung infections. Eur J Pharm Biopharm. 2017;117:363–371. doi:10.1016/j.ejpb.2017.04.032
   PubMed Web of Science ®Google Scholar
• Torge A, Wagner S, Chaves PS, et al. Ciprofloxacin-loaded lipid-core nanocapsules as mucus penetrating drug delivery system intended for the treatment of bacterial infections in cystic fibrosis. Int J Pharm. 2017;527(1–2):92–102. doi:10.1016/j.ijpharm.2017.05.013
   PubMed Web of Science ®Google Scholar
• Gunday Tureli N, Tureli AE, Schneider M. Inhalable antibiotic nanoformulations for the treatment of pseudomonas aeruginosa infection in cystic fibrosis – a review. Drug Deliv Lett. 2014;4(3):193–207. doi:10.2174/2210303104666140222002101
   Google Scholar
• Alipour M, Suntres ZE, Omri A. Importance of DNase and alginate lyase for enhancing free and liposome encapsulated aminoglycoside activity against Pseudomonas aeruginosa. J Antimicrob Chemother. 2009;64(2):317–325. doi:10.1093/jac/dkp165
   PubMed Web of Science ®Google Scholar
• Chemani C, Imberty A, de Bentzmann S, et al. Role of LecA and LecB Lectins in Pseudomonas aeruginosa-induced lung injury and effect of carbohydrate ligands. Infect Immun. 2009;77(5):2065–2075. doi:10.1128/IAI.01204-08
   PubMed Web of Science ®Google Scholar
• Baelo A, Levato R, Julián E, et al. Disassembling bacterial extracellular matrix with DNase-coated nanoparticles to enhance antibiotic delivery in biofilm infections. J Control Release. 2015;209:150–158. doi:10.1016/j.jconrel.2015.04.028
   PubMed Web of Science ®Google Scholar
• Hurley MN, Cámara M, Smyth AR. Novel approaches to the treatment of Pseudomonas aeruginosa infections in cystic fibrosis. Eur Respir J. 2012;40(4):1014. doi:10.1183/09031936.00042012
   PubMed Web of Science ®Google Scholar
• Reymond J-L, Bergmann M, Darbre T. Glycopeptide dendrimers as Pseudomonas aeruginosa biofilm inhibitors. Chem Soc Rev. 2013;42(11):4814–4822. doi:10.1039/c3cs35504g
   PubMed Web of Science ®Google Scholar
• Hamed MM, Abdelsamie AS, Rox K, et al. Towards translation of PqsR inverse agonists: from in vitro efficacy optimization to in vivo proof-of-principle. Adv Sci. 2023;10(5):2204443. doi:10.1002/advs.202204443
   Web of Science ®Google Scholar
• Wagner S, Sommer R, Hinsberger S, et al. Novel strategies for the treatment of pseudomonas aeruginosa infections. J Med Chem. 2016;59(13):5929–5969. doi:10.1021/acs.jmedchem.5b01698
   PubMed Web of Science ®Google Scholar
• Hartmann R, Steinbach A, Lu C, et al. Helmholtz-Zentrums für Infektionsforschung, Braunschweig, assignee. PqsR modulators; 2014.
   Google Scholar
• Lu C, Kirsch B, Maurer CK, et al. Optimization of anti-virulence PqsR antagonists regarding aqueous solubility and biological properties resulting in new insights in structure–activity relationships. Eur J Med Chem. 2014;79:173–183. doi:10.1016/j.ejmech.2014.04.016
   PubMed Web of Science ®Google Scholar
• Lu C, Kirsch B, Zimmer C, et al. Discovery of antagonists of PqsR, a key player in 2-alkyl-4-quinolone-dependent quorum sensing in Pseudomonas aeroginosae. Chem Biol. 2012;19(3):381–390. doi:10.1016/j.chembiol.2012.01.015
   PubMedGoogle Scholar
• Lu C, Maurer CK, Kirsch B, Steinbach A, Hartmann RW. Overcoming the unexpected functional inversion of a PqsR antagonist in Pseudomonas aeruginosa: an in vivo potent antivirulence agent targeting pqs quorum sensing. Angewandte Chemie. 2014;53(4):1109–1112. doi:10.1002/anie.201307547
   Google Scholar
• Schütz C, D-K H, Hamed MM, et al. A new PqsR inverse agonist potentiates tobramycin efficacy to eradicate pseudomonas aeruginosa biofilms. Adv Sci. 2021;8(12):2004369. doi:10.1002/advs.202004369
   Web of Science ®Google Scholar
• Schütz C, Hodzic A, Hamed M, et al. Divergent synthesis and biological evaluation of 2-(trifluoromethyl)pyridines as virulence-attenuating inverse agonists targeting PqsR. Eur J Med Chem. 2021;226:113797. doi:10.1016/j.ejmech.2021.113797
   PubMed Web of Science ®Google Scholar
• Nafee N, Husari A, Maurer CK, et al. Antibiotic-free nanotherapeutics: ultra-small, mucus-penetrating solid lipid nanoparticles enhance the pulmonary delivery and anti-virulence efficacy of novel quorum sensing inhibitors. J Control Release. 2014;192:131–140. doi:10.1016/j.jconrel.2014.06.055
   PubMed Web of Science ®Google Scholar
• Marangon CA, Martins VCA, Ling MH, et al. Combination of rhamnolipid and chitosan in nanoparticles boosts their antimicrobial efficacy. ACS Appl Mater Interfaces. 2020;12(5):5488–5499. doi:10.1021/acsami.9b19253
   PubMed Web of Science ®Google Scholar
• Alkawash MA, Soothill JS, Schiller NL. Alginate lyase enhances antibiotic killing of mucoid Pseudomonas aeruginosa in biofilms. Apmis. 2006;114(2):131–138. doi:10.1111/j.1600-0463.2006.apm_356.x
   PubMed Web of Science ®Google Scholar
• Yang M, Yang SX, Liu ZM, Li NN, Li L, Mou HJ. Rational Design of alginate lyase from microbulbifer sp. Q7 to improve thermal stability. Mar Drugs. 2019;17(6):378. doi:10.3390/md17060378
   PubMed Web of Science ®Google Scholar
• Yang M, Yu Y, Yang S, Shi X, Mou H, Li L. Expression and characterization of a New PolyG-specific alginate lyase from marine bacterium microbulbifer sp. Q7. Front Microbiol. 2018;9:2894. doi:10.3389/fmicb.2018.02894
   PubMed Web of Science ®Google Scholar
• Wan B, Zhu Y, Tao J, et al. Alginate lyase guided silver nanocomposites for eradicating Pseudomonas aeruginosa from lungs. ACS Appl Mater Interfaces. 2020;12(8):9050–9061. doi:10.1021/acsami.9b21815
   PubMed Web of Science ®Google Scholar
• Kamal AAM, Petrera L, Eberhard J, Hartmann RW. Structure–functionality relationship and pharmacological profiles of Pseudomonas aeruginosa alkylquinolone quorum sensing modulators. Org Biomol Chem. 2017;15(21):4620–4630. doi:10.1039/C7OB00263G
   PubMed Web of Science ®Google Scholar
• Shi C, Ahmad Khan S, Wang K, Schneider M. Improved delivery of the natural anticancer drug tetrandrine. Int J Pharm. 2015;479(1):41–51. doi:10.1016/j.ijpharm.2014.12.022
   PubMedGoogle Scholar
• Kirchner S, Fothergill JL, Wright EA, James CE, Mowat E, Winstanley C. Use of artificial sputum medium to test antibiotic efficacy against Pseudomonas aeruginosa in conditions more relevant to the cystic fibrosis lung. J Visualized Exp. 2012;64:e3857.
   Google Scholar
• Nafee N, Forier K, Braeckmans K, Schneider M. Mucus-penetrating solid lipid nanoparticles for the treatment of cystic fibrosis: proof of concept, challenges and pitfalls. Eur J Pharm Biopharm. 2018;124:125–137. doi:10.1016/j.ejpb.2017.12.017
   PubMed Web of Science ®Google Scholar
• Friedl H, Dünnhaupt S, Hintzen F, et al. Development and evaluation of a novel mucus diffusion test system approved by self-nanoemulsifying drug delivery systems. J Pharmaceut Sci. 2013;102(12):4406–4413. doi:10.1002/jps.23757
   PubMed Web of Science ®Google Scholar
• Stöber W. A note on the aerodynamic diameter and the mobility of non-spherical aerosol particles. J Aerosol Sci. 1971;2(4):453–456. doi:10.1016/0021-8502(71)90048-6
   Google Scholar
• Makled S, Boraie N, Nafee N. Nanoparticle-mediated macrophage targeting—a new inhalation therapy tackling tuberculosis. Drug Delivery Transl Res. 2021;11(3):1037–1055. doi:10.1007/s13346-020-00815-3
   PubMed Web of Science ®Google Scholar
• Song Y, Salinas D, Nielson DW, Verkman AS. Hyperacidity of secreted fluid from submucosal glands in early cystic fibrosis. Am J Physiol Cell Physiol. 2006;290(3):C741–C749. doi:10.1152/ajpcell.00379.2005
   PubMed Web of Science ®Google Scholar
• Gordillo-Galeano A, Mora-Huertas CE. Solid lipid nanoparticles and nanostructured lipid carriers: a review emphasizing on particle structure and drug release. Eur J Pharm Biopharm. 2018;133:285–308. doi:10.1016/j.ejpb.2018.10.017
   PubMed Web of Science ®Google Scholar
• Mohapatra BR. Biocatalytic characteristics of chitosan nanoparticle-immobilized alginate lyase extracted from a novel Arthrobacter species AD-10. Biocatal Agric Biotechnol. 2020;23:101458. doi:10.1016/j.bcab.2019.101458
   Web of Science ®Google Scholar
• Panigrahi KC, Patra CN, Jena GK, et al. Gelucire: a versatile polymer for modified release drug delivery system. Future J Pharm Sci. 2018;4(1):102–108. doi:10.1016/j.fjps.2017.11.001
   Web of Science ®Google Scholar
• Popat A, Liu J, Lu GQ, Qiao SZ. A pH-responsive drug delivery system based on chitosan coated mesoporous silica nanoparticles. J Mater Chem. 2012;22(22):11173–11178. doi:10.1039/c2jm30501a
   Web of Science ®Google Scholar
• Chronopoulou L, Massimi M, Giardi MF, et al. Chitosan-coated PLGA nanoparticles: a sustained drug release strategy for cell cultures. Colloids Surf B. 2013;103:310–317. doi:10.1016/j.colsurfb.2012.10.063
   PubMed Web of Science ®Google Scholar
• Mazzarino L, Borsali R, Lemos‐Senna E. Mucoadhesive films containing chitosan‐coated nanoparticles: a new strategy for buccal curcumin release. J Pharmaceut Sci. 2014;103(11):3764–3771. doi:10.1002/jps.24142
   PubMed Web of Science ®Google Scholar
• Mehnert W, Mäder K. Solid lipid nanoparticles: production, characterization and applications. Adv Drug Delivery Rev. 2001;47(2–3):165–196. doi:10.1016/S0169-409X(01)00105-3
   PubMed Web of Science ®Google Scholar
• Dumortier G, Grossiord JL, Agnely F, Chaumeil JC. A review of poloxamer 407 pharmaceutical and pharmacological characteristics. Pharm Res. 2006;23(12):2709–2728. doi:10.1007/s11095-006-9104-4
   PubMed Web of Science ®Google Scholar
• Si L, Yang S, Lin R, Gu S, Yan C, Yan J. SiO2–alginate–melittin nano-conjugates suppress the proliferation of ovarian cancer cells: a controlled release approach leveraging alginate lyase. Cancer Nanotechnol. 2024;15(1):4. doi:10.1186/s12645-023-00241-3
   Web of Science ®Google Scholar
• Nafee N, Taetz S, Schneider M, Schaefer UF, Lehr CM. Chitosan-coated PLGA nanoparticles for DNA/RNA delivery: effect of the formulation parameters on complexation and transfection of antisense oligonucleotides. Nanomedicine. 2007;3(3):173–183. doi:10.1016/j.nano.2007.03.006
   PubMed Web of Science ®Google Scholar
• Taetz S, Nafee N, Beisner J, et al. The influence of chitosan content in cationic chitosan/PLGA nanoparticles on the delivery efficiency of antisense 2’-O-methyl-RNA directed against telomerase in lung cancer cells. Eur J Pharm Biopharm. 2009;72(2):358–369. doi:10.1016/j.ejpb.2008.07.011
   PubMed Web of Science ®Google Scholar
• Huck BC, Hartwig O, Biehl A, et al. Macro- and microrheological properties of mucus surrogates in comparison to native intestinal and pulmonary mucus. Biomacromolecules. 2019;20(9):3504–3512. doi:10.1021/acs.biomac.9b00780
   PubMed Web of Science ®Google Scholar
• Pritchard Manon F, Powell Lydia C, Jack Alison A, et al. A low-molecular-weight alginate oligosaccharide disrupts pseudomonal microcolony formation and enhances antibiotic effectiveness. Antimicrob Agents Chemother. 2017;61(9):e00762–00717. doi:10.1128/AAC.00762-17
   PubMed Web of Science ®Google Scholar
• Finke JH, Richter C, Gothsch T, Kwade A, Büttgenbach S, Müller-Goymann CC. Coumarin 6 as a fluorescent model drug: how to identify properties of lipid colloidal drug delivery systems via fluorescence spectroscopy? Eur J Lipid Sci Technol. 2014;116(9):1234–1246. doi:10.1002/ejlt.201300413
   Web of Science ®Google Scholar
• Suk JS, Lai SK, Wang -Y-Y, et al. The penetration of fresh undiluted sputum expectorated by cystic fibrosis patients by non-adhesive polymer nanoparticles. Biomaterials. 2009;30(13):2591–2597. doi:10.1016/j.biomaterials.2008.12.076
   PubMed Web of Science ®Google Scholar
• Schlafer S, Meyer RL. Confocal microscopy imaging of the biofilm matrix. J Microbiol Methods. 2017;138:50–59. doi:10.1016/j.mimet.2016.03.002
   PubMed Web of Science ®Google Scholar
• Strathmann M, Wingender J, Flemming H-C. Application of fluorescently labelled lectins for the visualization and biochemical characterization of polysaccharides in biofilms of Pseudomonas aeruginosa. J Microbiol Methods. 2002;50(3):237–248. doi:10.1016/S0167-7012(02)00032-5
   PubMed Web of Science ®Google Scholar
• Messiaen AS, Forier K, Nelis H, Braeckmans K, Coenye T, Kaufmann GF. Transport of nanoparticles and tobramycin-loaded liposomes in Burkholderia cepacia complex biofilms. PLoS One. 2013;8(11):e79220. doi:10.1371/journal.pone.0079220
   PubMed Web of Science ®Google Scholar
• Ungaro F, d’Angelo I, Coletta C, et al. Dry powders based on PLGA nanoparticles for pulmonary delivery of antibiotics: modulation of encapsulation efficiency, release rate and lung deposition pattern by hydrophilic polymers. J Control Release. 2012;157(1):149–159. doi:10.1016/j.jconrel.2011.08.010
   PubMed Web of Science ®Google Scholar
• Tan Y, Ma S, Leonhard M, et al. Enhancing antibiofilm activity with functional chitosan nanoparticles targeting biofilm cells and biofilm matrix. Carbohydr Polym. 2018;200:35–42. doi:10.1016/j.carbpol.2018.07.072
   PubMed Web of Science ®Google Scholar
• Patel KK, Agrawal AK, Anjum MM, et al. DNase-I functionalization of ciprofloxacin-loaded chitosan nanoparticles overcomes the biofilm-mediated resistance of Pseudomonas aeruginosa. Appl Nanosci. 2020;10(2):563–575. doi:10.1007/s13204-019-01129-8
   Web of Science ®Google Scholar
• Nafee N, Gaber DM, Elzoghby AO, Helmy MW, Abdallah OY. Promoted antitumor activity of myricetin against lung carcinoma via nanoencapsulated phospholipid complex in respirable microparticles. Pharm Res. 2020;37(4):82. doi:10.1007/s11095-020-02794-z
   PubMed Web of Science ®Google Scholar
• Grace A, Sahu R, Owen DR, Dennis VA. Pseudomonas aeruginosa reference strains PAO1 and PA14: a genomic, phenotypic, and therapeutic review. Front Microbiol. 2022;13:1023523.
   PubMed Web of Science ®Google Scholar
• DeNegre AA, Myers K, Fefferman NH. Impact of strain competition on bacterial resistance in immunocompromised populations. Antibiotics. 2020;9(3):114. doi:10.3390/antibiotics9030114
   Google Scholar
• Chandler Courtney E, Horspool Alexander M, Hill Preston J, et al. Genomic and phenotypic diversity among ten laboratory isolates of Pseudomonas aeruginosa PAO1. J Bacteriol. 2019;201(5). doi:10.1128/jb.00595-00518
   PubMed Web of Science ®Google Scholar
• Mikkelsen H, McMullan R, Filloux A, Cornelis P. The Pseudomonas aeruginosa reference strain PA14 displays increased virulence due to a mutation in ladS. PLoS One. 2011;6(12):e29113–e29113. doi:10.1371/journal.pone.0029113
   PubMed Web of Science ®Google Scholar
• Sana TG, Lomas R, Gimenez MR, et al. Differential modulation of quorum sensing signaling through QslA in Pseudomonas aeruginosa Strains PAO1 and PA14. J Bacteriol. 2019;201(21). doi:10.1128/jb.00362-00319
   PubMed Web of Science ®Google Scholar
• Liu Y, Ahator SD, Wang H, et al. Microevolution of the mexT and lasR reinforces the bias of quorum sensing system in laboratory strains of Pseudomonas aeruginosa PAO1. Front Microbiol. 2022;13:821895.
   PubMed Web of Science ®Google Scholar
• Luria-Bertani LB. Liquid medium. Cold Spring Harbor Protocols. 2006;2006(1):pdb.rec8141.
   Google Scholar
• Korgaonkar AK, Whiteley M. Pseudomonas aeruginosa enhances production of an antimicrobial in response to N-acetylglucosamine and peptidoglycan. J Bacteriol. 2011;193(4):909–917. doi:10.1128/JB.01175-10
   PubMed Web of Science ®Google Scholar
• Neve Rachel L, Carrillo Brent D, Phelan Vanessa V. Impact of artificial sputum medium formulation on Pseudomonas aeruginosa secondary metabolite production. J Bacteriol. 2021;203(21):e00250–00221. doi:10.1128/JB.00250-21
   PubMed Web of Science ®Google Scholar
• Matilla Miguel A, Martín-Mora D, Gavira Jose A, Krell T. Pseudomonas aeruginosa as a model to study chemosensory pathway signaling. Microbiol Mol Biol Rev. 2021;85(1). doi:10.1128/mmbr.00151-00120
   PubMed Web of Science ®Google Scholar
• Rozenbaum R Antimicrobial and nanoparticle penetration and killing in infectious biofilms. Groningen, Rijksuniversiteit Groningen; 2019.
   Google Scholar
• Reighard KP, Hill DB, Dixon GA, Worley BV, Schoenfisch MH. Disruption and eradication of P. aeruginosa biofilms using nitric oxide-releasing chitosan oligosaccharides. Biofouling. 2015;31(9–10):775–787. doi:10.1080/08927014.2015.1107548
   PubMed Web of Science ®Google Scholar
• Gimmestad M, Sletta H, Ertesvåg H, et al. The Pseudomonas fluorescens AlgG Protein, but not its mannuronan C-5-epimerase activity, is needed for alginate polymer formation. J Bacteriol. 2003;185(12):3515–3523. doi:10.1128/JB.185.12.3515-3523.2003
   PubMed Web of Science ®Google Scholar
• Russell NJ, Gacesa P. Chemistry and biology of the alginate of mucoid strains of Pseudomonas aeruginosa in cystic fibrosis. Mol Aspect Med. 1988;10(1):1–91. doi:10.1016/0098-2997(88)90002-7
   PubMed Web of Science ®Google Scholar
• Boyd A, Chakrabarty AM. Role of alginate lyase in cell detachment of Pseudomonas aeruginosa. Appl Environ Microbiol. 1994;60(7):2355–2359. doi:10.1128/aem.60.7.2355-2359.1994
   PubMed Web of Science ®Google Scholar
• Lamppa JW, Griswold KE. Alginate lyase exhibits catalysis-independent biofilm dispersion and antibiotic synergy. Antimicrob Agents Chemother. 2013;57(1):137–145. doi:10.1128/AAC.01789-12
   PubMed Web of Science ®Google Scholar
• Meers P, Neville M, Malinin V, et al. Biofilm penetration, triggered release and in vivo activity of inhaled liposomal amikacin in chronic Pseudomonas aeruginosa lung infections. J Antimicrob Chemother. 2008;61(4):859–868. doi:10.1093/jac/dkn059
   PubMed Web of Science ®Google Scholar
• Alhajlan M, Alhariri M, Omri A. Efficacy and safety of liposomal clarithromycin and its effect on Pseudomonas aeruginosa virulence factors. Antimicrob Agents Chemother. 2013;57(6):2694–2704. doi:10.1128/AAC.00235-13
   PubMed Web of Science ®Google Scholar
• Drulis-Kawa Z, Gubernator J, Dorotkiewicz-Jach A, Doroszkiewicz W, Kozubek A. In vitro antimicrobial activity of liposomal meropenem against Pseudomonas aeruginosa strains. Int J Pharm. 2006;315(1–2):59–66. doi:10.1016/j.ijpharm.2006.02.017
   PubMedGoogle Scholar
• Jesus S, Schmutz M, Som C, Borchard G, Wick P, Borges O. Hazard assessment of polymeric nanobiomaterials for drug delivery: what can we learn from literature so far. Front Bioeng Biotechnol. 2019;7:261. doi:10.3389/fbioe.2019.00261
   PubMed Web of Science ®Google Scholar
• Yermak IM, Davidova VN, Gorbach VI, et al. Forming and immunological properties of some lipopolysaccharide–chitosan complexes. Biochimie. 2006;88(1):23–30. doi:10.1016/j.biochi.2005.07.004
   PubMed Web of Science ®Google Scholar
• Yamada H, Koike N, Ehara T, Matsumoto T. Measuring antimicrobial susceptibility of Pseudomonas aeruginosa using Poloxamer 407 gel. J Infect Chemother. 2011;17(2):195–199. doi:10.1007/s10156-010-0109-x
   PubMed Web of Science ®Google Scholar
• Taylor BJ, Marsh LL, Nosworthy JO, Williams DW. A novel approach to antibiofilm susceptibility testing using a thermo-reversible matrix. J Wound Care. 2016;25(2):62–67. doi:10.12968/jowc.2016.25.2.62
   PubMed Web of Science ®Google Scholar
• Bose SK, Nirbhavane P, Batra M, Chhibber S, Harjai K. Nanolipoidal α-terpineol modulates quorum sensing regulated virulence and biofilm formation in Pseudomonas aeruginosa. Nanomedicine. 2020;15(18):1743–1760. doi:10.2217/nnm-2020-0134
   PubMed Web of Science ®Google Scholar
• Cheow WS, Chang M, Hadinoto K. The roles of lipid in anti-biofilm efficacy of lipid-polymer hybrid nanoparticles encapsulating antibiotics. Colloids Surf A. 2011;389(1–3):158–165.
   Google Scholar
• Badawy MSEM, Riad OKM, Taher FA, Zaki SA. Chitosan and chitosan-zinc oxide nanocomposite inhibit expression of LasI and RhlI genes and quorum sensing dependent virulence factors of Pseudomonas aeruginosa. Int J Biol Macromol. 2020;149:1109–1117. doi:10.1016/j.ijbiomac.2020.02.019
   PubMed Web of Science ®Google Scholar
• Germoni L, Bremer P, Lamont I. The effect of alginate lyase on the gentamicin resistance of Pseudomonas aeruginosa in mucoid biofilms. J Appl Microbiol. 2016;121(1):126–135. doi:10.1111/jam.13153
   PubMed Web of Science ®Google Scholar
• Rivera Aguayo P, Bruna Larenas T, Alarcón Godoy C, et al. Antimicrobial and antibiofilm capacity of chitosan nanoparticles against wild type strain of Pseudomonas sp. isolated from milk of cows diagnosed with bovine mastitis. Antibiotics. 2020;9(9):551. doi:10.3390/antibiotics9090551
   Google Scholar
• Kragh KN, Alhede M, Kvich L, Bjarnsholt T. Into the well—A close look at the complex structures of a microtiter biofilm and the crystal violet assay. Biofilm. 2019;1:100006. doi:10.1016/j.bioflm.2019.100006
   PubMedGoogle Scholar
• Quintas V, Prada-López I, Tomás I. Analyzing the Oral Biofilm Using Fluorescence-Based Microscopy: What’s in a Dye? Formatex Research Center; 2014.
   Google Scholar
• Méndez-Vilas ADJ. Microscopy: Science, Technology, Applications and Education. Badajoz: Formatex Research Center; 2010.
   Google Scholar
• Rizzi Yanina S, Happel P, Lenz S, et al. Chitosan and chitin deacetylase activity are necessary for development and virulence of Ustilago maydis. mBio. 2021;12(2). doi:10.1128/mbio.03419-03420
   PubMed Web of Science ®Google Scholar
• Somashekar D, Joseph R. A new spectrophotometric method of assay for chitosanase based on calcofluor white dye binding. Carbohydr Polym. 1997;34(4):343–346. doi:10.1016/S0144-8617(97)00052-0
   Web of Science ®Google Scholar
• Davydova VN, Yermak IM, Gorbach VI, Krasikova IN, Solov’eva TF. Interaction of bacterial endotoxins with chitosan. Effect of endotoxin structure, chitosan molecular mass, and ionic strength of the solution on the formation of the complex. Biochem Biokhimiia. 2000;65(9):1082–1090.
   PubMed Web of Science ®Google Scholar