391
Views
2
CrossRef citations to date
0
Altmetric
PAPERS

Identification of active site residues in the Shigella flexneri glucosyltransferase GtrV

, , &
Pages 104-113 | Received 06 Sep 2009, Accepted 17 Dec 2009, Published online: 25 Mar 2010

References

  • Alexeyev MF, Winkler HH. 1999. Membrane topology of the Rickettsia prowazekii ATP/ADP translocase revealed by novel dual pho-lac reporters. J Mol Biol 285(4):1503–1513.
  • Alexeyev MF, Winkler HH. 2002. Transposable dual reporters for studying the structure-function relationships in membrane proteins: permissive sites in R. prowazekii ATP/ADP translocase. Biochemistry 41(1):406–414.
  • Allison GE, Verma NK. 2000. Serotype-converting bacteriophages and O-antigen modification in Shigella flexneri. Trends Microbiol 8(1):17–23.
  • Campanella JJ, Bitincka L, Smalley J. 2003. MatGAT: an application that generates similarity/identity matrices using protein or DNA sequences. BMC Bioinformatics 4:29.
  • Cantarel BL, Coutinho PM, Rancurel C, Bernard T, Lombard V, Henrissat B. 2009. The Carbohydrate-Active EnZymes database (CAZy): an expert resource for Glycogenomics. Nucleic Acids Res 22:480–484.
  • Crouvoisier M, Auger G, Blanot D, Mengin-Lecreulx D. 2007. Role of the amino acid invariants in the active site of MurG as evaluated by site-directed mutagenesis. Biochimie 89(12):1498–1508.
  • Cserzo M, Wallin E, Simon I, von Heijne G, Elofsson A. 1997. Prediction of transmembrane alpha-helices in prokaryotic membrane proteins: the dense alignment surface method. Protein engineering 10(6):673–676.
  • Daniels C, Vindurampulle C, Morona R. 1998. Overexpression and topology of the Shigella flexneri O-antigen polymerase (Rfc/Wzy). Mol Microbiol 28(6):1211–1222.
  • Garinot-Schneider C, Lellouch AC, Geremia RA. 2000. Identification of essential amino acid residues in the Sinorhizobium meliloti glucosyltransferase ExoM. J Biol Chem 275(40):31407–31413.
  • Guan S, Bastin DA, Verma NK. 1999. Functional analysis of the O antigen glucosylation gene cluster of Shigella flexneri bacteriophage SfX. Microbiology 145(Pt 5):1263–1273.
  • Hitchcock PJ, Brown TM. 1983. Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide gels. J Bacteriol 154(1):269–277.
  • Hofmann K, and W. Stoffel. 1993. TMbase – A database of membrane spanning proteins segments. Biol Chem Hoppe-Seyler 374:166.
  • Johnson M, Zaretskaya I, Raytselis Y, Merezhuk Y, McGinnis S, Madden TL. 2008. NCBI BLAST: a better web interface. Nucleic Acids Res 36(Web Server issue):W5–9.
  • Korres H, Mavris M, Morona R, Manning PA, Verma NK. 2005. Topological analysis of GtrA and GtrB proteins encoded by the serotype-converting cassette of Shigella flexneri. Biochem Biophys Res Commun 328(4):1252–1260.
  • Korres H, Verma NK. 2004. Topological analysis of glucosyltransferase GtrV of Shigella flexneri by a dual reporter system and identification of a unique reentrant loop. J Biol Chem 279(21):22469–22476.
  • Korres H, Verma NK. 2006. Identification of essential loops and residues of glucosyltransferase V (GtrV) of Shigella flexneri. Mol Membr Biol 23(5):407–419.
  • Kotloff KL, Winickoff JP, Ivanoff B, Clemens JD, Swerdlow DL, Sansonetti PJ, Adak GK, Levine MM. 1999. Global burden of Shigella infections: implications for vaccine development and implementation of control strategies. Bull World Health Organ 77(8):651–666.
  • Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA, McWilliam H, Valentin F, Wallace IM, Wilm A, Lopez R, Thompson JD, Gibson TJ, Higgins DG. 2007. Clustal W and Clustal X version 2.0. Bioinformatics 23(21):2947–2948.
  • Lehane AM, Korres H, Verma NK. 2005. Bacteriophage-encoded glucosyltransferase GtrII of Shigella flexneri: membrane topology and identification of critical residues. Biochem J 389(Pt 1):137–143.
  • Liu B, Knirel YA, Feng L, Perepelov AV, Senchenkova SN, Wang Q, Reeves PR, Wang L. 2008. Structure and genetics of Shigella O antigens. FEMS Microbiol Rev 32(4):627–653.
  • Marks DL, Dominguez M, Wu K, Pagano RE. 2001. Identification of active site residues in glucosylceramide synthase. A nucleotide-binding catalytic motif conserved with processive beta-glycosyltransferases. J Biol Chem 276(28):26492–26498.
  • Sambrook J, Russell DW. 2001. Molecular cloning: a laboratory manual. 3rd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
  • Van den Bosch L, Manning PA, Morona R. 1997. Regulation of O-antigen chain length is required for Shigella flexneri virulence. Mol Microbiol 23(4):765–775.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.