120
Views
4
CrossRef citations to date
0
Altmetric
ORIGINAL ARTICLE

Modification of Alcalase SphereZyme™ by entrapment in LentiKats® to impart improved particle stability

, , &
Pages 71-78 | Received 07 Mar 2012, Accepted 14 Jan 2013, Published online: 01 Mar 2013

References

  • Betancor L, Fuentes M, Dellamora-Ortiz G, López-Gallego F, Hidalgo A, Alonso-Morales N, Mateo C, Guisán JM, Fernández-Lafuente R. 2005. Dextran aldehyde coating of glucose oxidase immobilised on magnetic nanoparticles prevents its inactivation by gas bubbles. J Mol Catal B Enz 32:97–101.
  • Bradford MM. 1976. Rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254.
  • Brady D, Steenkamp L, Reddy S, Skein E, Chaplin J. 2004. Optimisation of the enantioselective biocatalytic hydrolysis of naproxen ethyl ester using ChiroCLEC CR. Enzyme Microb Technol 34:283–291.
  • Brady D, Jordaan J, Simpson C, Chetty A, Arumugam C, Moolman FS. 2008. SphereZyme™ a novel structured self- immobilisation enzyme technology. BMC Biotechnol 8:8.
  • Brady D, Jordaan J. 2009. Advances in enzyme immobilisation. Biotech Lett 31:1639–1650.
  • Cao L, Van Rantwijk F, Sheldon RA. 2000. Cross-linked enzyme aggregates: a simple and effective method for the immobilisation of penicillin acylase. Org Lett 2:1361–1364.
  • Cao L, Langen LV, Sheldon RA. 2003. Immobilised enzymes: carrier-bound or carrier-free?Curr Opin Biotechnol 14:387–394.
  • Cao L, Elzinga J. 2003. Crosslinked enzyme aggregates and crosslinking agent therefore. Patent WO 2003/066850.
  • Caramori SS, Fernandes KF. 2008. The use of poly(ethylene terephthalate)-poly(aniline) composite for trypsin immobilisation. Mat Sci Eng C 28:1159–1163.
  • Corîci LN, Frissen AE, Van Zoelen DJ, Eggen IF, Peter F, Davidescu CM, Boeriu CG. 2011. Sol-gel immobilization of Alcalase from Bacillus licheniformis for application in the synthesis of C-terminal peptide amides. J Mol Catal B Enzym 73:90–97.
  • Cruz J, Barbosa O, Rodrigues RC, Fernandez-Lafuente R, Torres R, Ortiz C. 2012. Optimized preparation of CALB-CLEAs by response surface methodology: the necessity to employ a feeder to have an effective crosslinking. J Mol Catal B Enz 80:7–14.
  • D'ambrosio A, Rossano R, Ungaro N, Riccio P. 2003. Proteolytic and milk clotting activities in extracts obtained from the crustaceans Munida. J Mol Cat B Enzym 22:145–150.
  • Fernandes P, Marques MPC, Carvalho F, Cabral JMS. 2009. A simple method for biocatalyst immobilization using PVA-based hydrogel. J Chem Technol Biotechnol 84:561–564.
  • Ferreira L, Ramos MA, Dordick JS, Gil MH. 2003. Influence of different silica derivatives in the immobilization and stabilization of a Bacillus licheniformis protease (Subtilisin Carlsberg). J Mol Catal B Enzym 21:189–199.
  • Garcia-Galan C, Berenguer-Murcia A, Fernandez-Lafuente R, Rodrigues RC. 2011. Potential of different enzyme immobilization strategies to improve enzyme performance. Adv Synth Catal 353:2885–2904.
  • Hamerska-Dudra A, Bryjak J, Trochimczuk AW. 2007. Immobilisation of glucoamylase and trypsin on crosslinked thermosensitive carriers. Enz Microb Technol. 41:197–204.
  • Harris JM, Struck EC, Case MG, Paley MS, Yalpani M, Van Alstine JM, Brooks DE. 1984. Synthesis and characterization of poly(ethylene glycol) derivatives. J Polym Sci Pol Chem 22:341–352.
  • Iversen SL, Jørgensen MH. 1995. Azocasein assay for alkaline protease in complex fermentation broth. Biotechnol Tech 9:573–576.
  • Jordaan J, Mathye S, Simpson C, Brady D. 2009. Improved chemical and physical stability of laccase after SphereZyme™ immobilisation. Enz Microb Technol 45:432–435.
  • Kawaguti HY, Buzzato MF, Orsi DC, Suzuki GT, Sato HH. 2006. Effect of the additives polyethyleneimine and glutaraldehyde on the immobilisation of Erwinia sp. D12 cells in calcium alginate for isomaltulose production. Process Biochem 41:2035–2040.
  • López-Gallego F, Betancor L, Hidalgo A, Alonso N, Fernandez-Lafuente R, Guisan JM. 2005. Co-aggregation of enzymes and polyethyleneimine: A simple method to prepare stable and immobilised derivatives of glutaryl acylase. Biomacromolecules 6:1839–1842.
  • López-Gallego F, Betancor L, Hidalgo A, Dellamora-Ortiz G, Mateo C, Fernández-Lafuente R, Guisán JM. 2007. Stabilization of different alcohol oxidases via immobilisation and post immobilisation techniques. Enz Microb Technol 40:278–284.
  • Mateo C, Palomo JM, Van Langen LM, Van Rantwijk F, Sheldon RA. 2004. A new, mild cross-linking methodology to prepare cross-linked enzyme aggregates. Biotechnol Bioeng 86:273–276.
  • Migneault I, Dartiguenave C, Bertrand MJ, Waldron KC. 2004. Glutaraldehyde: behavior in aqueous solution, reaction with proteins, and application to enzyme crosslinking, BioTechniques 37:790–802.
  • Moolman FS, Brady D, Rolfes H, Sewlall AS, Jordaan J. 2005. Stabilisation of enzymes. Patent WO 2005/080561.
  • Payne JW. 1973. Polymerization of proteins with glutaraldehyde: Soluble molecular weight markers. Biochem J 135:867–873.
  • Polgár L, Bender ML. 1969. The nature of general base-general acid catalysis in serine proteases. PNAS 64:1335–1342.
  • Ramos VM, Rodríguez NM, Henning I, Díaz MF, Monachesi MP, Rodríguez MS, Abarrategi A, Correas-Magaña V, López-Lacomba JL, Agulló E. 2006. Poly(ethylene glycol)-crosslinked N-methylene phosphonic chitosan: Preparation and characterization. Carbohyd Polym 64:328–336.
  • Roberts MJ, Bentley MD, Harris JM. 2002. Chemistry for peptide and protein PEGylation. Adv Drug Del Rev 54:459–476.
  • Rousseau AL, Buddoo SR, Gordon GER, Beemadu S, Kupi BG, Lepuru MJ, Maumela MC, Parsoo A, Sibiya DM, Brady D. 2011. A chemo-enzymatic process for stereoselective synthesis of monatin. OPRD 15:249–257.
  • Sangeetha K, Emilia Abraham T. 2008. Preparation and characterization of cross-linked enzyme aggregates (CLEA) of Subtilisin for controlled release applications. Int J Biol Macromol 43:314–319.
  • Sheldon RA, Schoevaart R, van Langen LM. 2005. Cross-linked enzyme aggregates (CLEAs): a novel and versatile method for enzyme immobilization (a review). Biocatal Biotrans 23: 141–147.
  • Shah S, Sharma A, Gupta MN. 2006. Preparation of cross-linked enzyme aggregates by using bovine serum albumin as a proteic feeder. Anal Biochem 351:207–213.
  • Sheldon RA, Schoevaart R, van Langen LM. 2005. Cross-linked enzyme aggregates (CLEAs): a novel and versatile method for enzyme immobilization (a review). Biocatal Biotrans 23:141–147.
  • St.Clair NL, Navia MA. 1992. Cross-linked enzyme crystals as robust biocatalysts. J Am Chem Soc 114:7314–7316.
  • Sheldon RA. 2011. Cross-linked enzyme aggregates as industrial biocatalysts. OPRD 15:213–223.
  • Simi KC, Abraham TE. 2007. Encapsulation of crosslinked subtilisin microcrystals in hydrogel beads for controlled release applications. Euro J Pharm Sci 32:17–23.
  • Tardioli PW, Pedroche J, Raquel LC, Giordano RLC, Fernández-Lafuente R, Guisán JM. 2003. Hydrolysis of proteins by immobilized-stabilized Alcalase-glyoxyl agarose. Biotechnol Progress 19:352–360.
  • Tardioli PW, Sousa R Jr, Giordano RC, Giordano RLC. 2005. Kinetic model of the hydrolysis of polypeptides catalyzed by Alcalase® immobilized on 10% glyoxyl-agarose. Enz Microb Technol 36:555–564.
  • Tischer W, Wedekind F. 1999. Immobilized enzymes: methods and applications. Topics Curr Chem 200:96–126.
  • Twala BV, Sewell BT, Jordaan J. 2012. Immobilisation and characterisation of biocatalytic co-factor recycling enzymes, glucose dehydrogenase and NADH oxidase, on aldehyde functional ReSyn™polymer microspheres. Enz Microb Technol 50:331–336.
  • Veronese FM. 2001. Peptide and protein PEGylation: a review of problems and solutions. Biomaterials 22:405–417.
  • Wang YF, Yakovlevsky K, Khalaf N, Zhang B, Margolin AL. 1996. Cross-linked crystals of subtilisin. Versatile catalysts for organic synthesis. Ann New York Acad Sci 799:777–783.
  • Wang YF, Yakovlevsky K, Zhang B, Margolin AL. 1997. Cross-linked crystals of subtilisin: Versatile catalyst for organic synthesis. J Org Chem 62:3488–3495.
  • Wilson L, Illanes A, Pessela BCC, Abián O, Fernández-Lafuente R, Guisán J. 2004. Encapsulation of cross-linked Penicillin G Acylase aggregates in LentiKats: evaluation of a novel biocatalyst in organic media. Biotechnol Bioeng 80:558–562.
  • Wilson L, Illanes A, Soler L, Henríquez MJ. 2009. Effect of the degree of cross-linking on the properties of different CLEAs of penicillin acylase. Process Biochem 44:322–326.
  • Zhang Y, Wu H, Li J, Li L, Jiang Y, Jiang Y, Jiang Z. 2008. Protamine-templated biomimetic hybrid capsule: Efficient and stable carrier for enzyme encapsulation. Chem Materials 20:1041–1048.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.