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Original Article

Enzyme Clearing of Alcian Blue Stained Whole Small Vertebrates for Demonstration of Cartilage

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Pages 229-232 | Published online: 12 Jul 2009

References

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  • Simons E. V., Van Horn J. R. A new procedure for whole-mount alcian blue staining of chicken embryos, adapted to the clearing procedure in potassium hydroxide. Acta Morphol. Neerl.-Scand. 1971a; 8: 281–292
  • Simons E. V., Van Horn J. R. An in-toto staining procedure for the cartilaginous skeleton of chicken embryos. Acta Morphol. Neerl-Scand. 1971b; 9: 138–139
  • Taylor W. R. An enzyme method of clearing and staining small vertebrates. Proc. U. S. Natl. Mus. 1967a; 122(3596)1–17
  • Taylor W. R. Outline of a method of clearing tissues with pancreatic enzymes and staining bones of small vertebrates. Turtox News 1967b; 45: 308–309
  • Wassersug R. J. A procedure for differential staining of cartilage and bone in whole formalin-fixed vertebrates. Stain Technol. 1976; 51: 131–134

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