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ORIGINAL ARTICLE

2.45-GHz microwave irradiation adversely affects reproductive function in male mouse, Mus musculus by inducing oxidative and nitrosative stress

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Pages 511-525 | Received 17 Jul 2013, Accepted 26 Jan 2014, Published online: 25 Feb 2014

References

  • Alhekail ZO. Electromagnetic radiation from microwave ovens. J Radiol Prot 2001;21:251–258.
  • International Commission on Non-Ionizing Radiation Protection (ICNIRP). Medical magnetic resonance (MR) procedures: Protection of patients. Health Phys 2004;87:197–216.
  • Institute of Electrical and Electronic Engineers (IEEE). IEEE Standard for Safety Levels with respect to Human Exposure to Radio Frequency Electromagnetic Fields, 3 kHz to 300 GHz. New York: IEEE Topical Review 2005; R 282.
  • Rao RGV, Cain CA, Lockwood V, Tompkins WAF. Effects of microwave exposure on hamsters immune system II: Pentoneal macrophage function. Bioelectromagnetics 1983;4:141–155.
  • Veyret B. Antibody response of mice to low-power MW under combined, pulsed and amplitude modulation. Bioelectromagnetics 1983;87:375–380.
  • Aweda MA, Gbenebitse SO, Meindinyo RO. Effects of 2.45 GHz MW exposures on the peroxidation status in Wistar rats. Niger Postgrad Med J 2003;10:243–246.
  • Azinge EC, Mabayoje M, Sofola OA. LDL peroxidation and total antioxidant status in Nigerian patients with sickle-cell disease. Nig J Hosp Med 2001;11:46–50.
  • Awobajo FO, Raji Y, Olatunji-Bello II, Kunle-Alabi FT, Adesanya AO, Awobajo TO. Fourteen days oral administration of therapeutic dosage of some Antibiotics reduced serum Testosterone in male rats. Niger J Health Biomed Res 2006;5:17–20.
  • Raji Y, Kunle-Alabi OT, Olaleye SB, Gbadegesin MA, Awobajo FO, Osonuga OA, et al. Impact of α-tocopherol on Metronidazole and Tetracycline-induced Alteration in Reproductive Activities of Male Albino Rats. J Biol Sci 2007;7:41–46.
  • Fejes I, Zavaczki Z, Szollosi J, Koloszar S, Daru J, Kovaks L, Pal A. Is there a relationship between cell phone use and semen quality?Arch Androl 2005;51:385–393.
  • Kilgallon SJ, Simmons LW. Image content influences men's semen quality. Biol Lett 2005;1:253–255.
  • Erogul O, Oztas E, Yildirim I, Kir T, Aydur E, Komesli G, et al. Effects of electromagnetic radiation from a cellular phone on human sperm motility: an in vitro study. Arch Med Res 2006;37:840–843.
  • Agarwal A, Deepinder F, Rakesh K, Sharma RK, Ranga G, Li J. Effect of cell phone usage on semen analysis in men attending infertility clinic: an observational study. Fertil Steril 2008a;89:124–128.
  • Agarwal A, Desai NR, Makker K, Varghese A, Mouradi R, Sabanegh E, Sharma R. Effects of radiofrequency electromagnetic waves (RF-EMW) from cellular phones on human ejaculated semen: an in vitro pilot study. Fertil Steril 2008b; 90:S337–S338.
  • Singh VP, Singh P, Chaturvedi CM, Shukla RK, Dhawan A, Gangwar RK, Singh SP. 2.45 GHz low level CW microwave radiation affects embryo implantation sites and single strand DNA damage in brain cell of mice, Mus musculus. Proceedings of 2009 International Conference on Emerging Trends in Electronic and Photonic Devices & Systems (ELECTRO-2009) from IEEE Explore 2009; 22–24 December, Institute of Technology, Banaras Hindu University, Varanasi, India. pp. 379–382.
  • Shahin S, Singh VP, Shukla RK, Dhawan A, Gangwar RK, Singh SP, Chaturvedi CM. 2.45 GHz Microwave Irradiation Induced Oxidative Stress Affects Implantation or Pregnancy in Mice, Mus musculus. Appl Biochem Biotechnol 2013;169: 727–1751.
  • Gunn SA, Gould TC, Anderson WAD. The effect of microwave radiation on morphology and function of rat testes. Lab Invest 1961;10:301–314.
  • Saunders RD, Kowalczuk CI. Effects of 2.45 GHz microwave radiation and heat on mouse spermatogenic epithelium. Int J Radiat Biol Relat Stud Phys Chem Med 1981;40:623–632.
  • Kowalczuk CI, Saunders RD, Stapleton HR. Sperm count and sperm abnormality in male mice after exposure to 2.45 GHz microwave radiation. Mutat Res. 1983;122:155–161.
  • Lebovitz RM, Johnson L, Samson WK. Effects of pulse-modulated microwave radiation and conventional heating on sperm production. J Appl Physiol 1987;62:245–252.
  • Magras IN, Xenos TD. RF radiation-induced changes in the prenatal development of mice. Bioelectromagnetics 1997;18: 455–461.
  • Akdag MZ, Celik MS, Ketani A, Nergiz Y, Deniz M, Dasdag S. Effect of chronic low-intensity microwave radiation on sperm count, sperm morphology, and testicular epididymal tissues of rats. Electro- Magnetobiol 1999;18:135–145.
  • Stone JR, Yang S. Hydrogen peroxide: a signalling messenger. Antioxid Redox Signal 2006;8:243–270.
  • Passos JF, Saretzki G, Ahmed S, Nelson G, Richter T, Peters H, et al. Mitochondrial dysfunction accounts for the stochastic heterogeneity in telomere dependent senescence. PLoS Biol. 2007;5:110.
  • de Rooij DG, van de Kant HJG, Dol R, Gerard Wagemaker G, van Buul PPW, van Duijn-Goedhart A, et al. Long-term effects of irradiation before adulthood on reproductive function in the male rhesus monkey. Biol Reprod 2002;66:486–494.
  • Moller P, Wallin H, Knudsen LE. Oxidative stress associated with exercise, psychological stress and life-style factors. Chem Biol Interact 1996;102:17–36.
  • Aitken RJ. Molecular mechanisms regulating human sperm function. Mol Hum Reprod 1997;3:169–173.
  • Gagnon C, Iwasaki A, De Lamirande E, Kovalski N. Reactive oxygen species and human spermatozoa. Ann N Y Acad Sci 1991;637:436–444.
  • Agarwal A, Nallella KP, Allamaneni SS, Said TM. Role of antioxidants in treatment of male infertility: an overview of the literature. Reprod Biomed Online 2004;8:616–627.
  • Griveau JF, Le Lannou D. Reactive oxygen species and human spermatozoa: physiology and pathology. Int J Androl 1997;20:61–69.
  • Agarwal A, Deepinder F, Sharma RK, Ranga G, Li J. Effect of cell phone usage on semen analysis in men attending infertility clinic: an observational study. Fertil Steril 2008; 89:124–128.
  • Erenpreiss J, Hlevicka S, Zalkalns J, Erenpreisa J. Effect of leukocytospermia on sperm DNA integrity: a negative effect in abnormal semen samples. J Androl 2002;23: 717–723.
  • Marchetti C, Obert G, Deffosez A, Formstecher P, Marchetti P. Study of mitochondrial membrane potential, reactive oxygen species, DNA fragmentation and cell viability by flow cytometry in human sperm. Hum Reprod 2002;17:1257–1265.
  • Saleh RA, Agarwal A, Kandirali E, Sharma RK, Thomas AJ, Nada EA. Leukocytospermia is associated with increased reactive oxygen species production by human spermatozoa. Fertil Steril 2002;78:1215–1224.
  • Kesari KK, Behari J. Microwave exposure affecting reproductive system in male rats. Appl Biochem Biotechnol 2010; 162:416–428.
  • Kesari KK, Kumar S, Behari J. Mobile phone usage and male infertility in Wistar rats. Indian J Exp Biol 2010;48: 987–992.
  • Kesari KK, Kumar S, Behari J. Effects of radiofrequency electromagnetic waves exposure from cellular phone on reproductive pattern in male Wistar rats. Appl Biochem Biotechnol 2011;164:546–559.
  • Kesari KK, Behari J. Fifty-gigahertz microwave exposure effect of radiations on rat brain. Appl Biochem Biotechnol 2011;158:126–139.
  • Kumar S, Kesari KK, Behari J. Influence of microwave exposure on fertility of male rats. Fertil Steril 2011;95: 1500–1502.
  • Chen ZN. Antennas for Portable Devices. Chichester, England: Wiley; 2007, p. 113.
  • Chaturvedi CM, Singh VP, Singh P, Basu P, Singaravel M, Shukla RK, et al. 2.45 GHz (CW) Microwave irradiation alters circadian organization, spatial memory, DNA structure in the brain cells and blood cell counts of male mice, Mus musculus. Prog Electromagn Res B 2011;29:23–42.
  • World Health Organization. WHO Laboratory Manual for the Examination of Human Semen and Semen–cervical Mucus Interaction. Cambridge, UK: Cambridge University Press; 1999.
  • Bejma J, Ramires P, Ji LL. Free radical generation and oxidative stress with ageing and exercise: differential effects in the myocardium and liver. Acta Physiol Scand 2000;169: 343–351.
  • Sastry KV, Moudgal RP, Mohan J, Tyagi JS, Rao GS. Spectrophotometric determination of serum nitrite and nitrate by Copper. Anal Biochem 2002;306:79–82.
  • Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979;95:351–358.
  • Beauchamp C, Fridovich I. Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal Biochem 1971;44:276–287.
  • Sun Y, Elwell JH, Oberley LW. A simultaneous visualization of the antioxidant enzymes glutathione peroxidase and catalase on polyacrylamide gels. Free Radical Res Commun 1988;5:67–75.
  • Lin CL, Chen HJ, Hou WC. Activity staining of glutathione peroxidase after electrophoresis on native and sodium dodecylsulfate polyacrylamide gels. Electrophoresis 2002;23:513–516.
  • Shivanandappa T, Venkatesh S. A colorimetrie assay method for3beta-hydroxy-delta5-steroid dehydrogenase. Annal Biochem 1997;254:57–61.
  • Srivastava R, Cornett LE, Chaturvedi CM. Effect of photoperiod and estrogen on 740 expression of arginine vasotocin and its oxytocic-like receptor in the shell 741 gland of the Japanese quail. Comp Biochem Physiol Part A 2007;148:451–457.
  • Sharpe RM, Donachie K, Cooper I. Re-evaluation of the intratesticular level of testosterone required for quantitative maintenance of spermatogenesis in the rat. J Endocrinol 1998;117: 19–26.
  • Sharpe RM, Maddocks S, Millar M, Saunders PTK, Kerr JB, Mckinnell C. Testosterone and spermatogenesis: identification of stage dependent, androgen- regulated proteins secreted by adult rat seminiferous tubules. J Androl 1992;13:172–184.
  • Yan SW, Zhang N, Tang J, Lu HO, Wang XL. Long-term exposure to low intensity microwave radiation affects male reproductivity. Zhonghua Nan Ke Xue 2007;13:306–308.
  • Kim JM, Ghosh SR, Weil ACP, Zirkin BR. Caspase- 3 and caspaseactivated dioxyribonuclease are associated with testicular germ cell apoptosis resulting from reduced intratesticular testosterone. Endocrinology 2001;142:3809–3816.
  • Blanco-Rodriguez J, Martinez-Garcia C. Apoptosis precedes detachment of germ cells from the seminiferous epithelium after hormonal suppression by short-term oestradiol treatment of rats. Int J Androl 1998;21:109–115.
  • Batellier F, Couty I, Picard D, Brillard JP. Effects of exposing chicken eggs to a cell phone in “call” position over the entire incubation period. Theriogenology 2008;69:737–745.
  • Otitoloju AA, Obe IA, Adewale OA, Otubanjo OA, Osunkalu VO. Preliminary study on the induction of sperm head abnormalities in mice, Mus musculus, exposed to radiofrequency radiations from global system for mobile communication base stations. Bull Environ Contam Toxicol 2010;84:51–54.
  • Varma MM, Traboulay EA Jr. Biological effects of microwave radiation on the testes of Swiss mice. Experientia 1975;31: 301–302.
  • Desai NR, Kesari KK, Agarwal A. Pathophysiology of cell phone radiation: oxidative stress and carcinogenesis with focus on male reproductive system. Reprod Biol Endocrinol 2009;7:114.
  • Agarwal A, Singh A, Hamada A, Kesari KK. Cell phones and male infertility: a review of recent innovations in technology and consequences. Int Braz J Urol 2011;37:432–454.
  • Sikka SC. Relative impact of oxidative stress on male reproductive function. Curr Med Chem 2001;8:851–862.
  • Jeulin C, Soufir JC, Weber P, Laval-Martin D, Calvayrac R. Catalase activity in human spermatozoa and seminal plasma. Gamete Res 1989;24:185–196.
  • Alkan I, Simsek F, Haklar G, Kervancioglu E, Ozveri H, Yalcin S, Akdas A. Reactive oxygen species production by the spermatozoa of patients with idiopathic infertility: relationship to seminal plasma antioxidants. J Urol 1997;157:140–143.
  • Miesel R, Jedrzejczak P, Sanocka D, Kurpisz MK. Severe antioxidase deficiency in human semen samples with pathological spermiogram parameters. Andrologia 1997;29: 77–83.
  • Sanocka D, Miesel R, Jedrzejczak P, Chelmonska-Soyta AC, Kurpisz M. Effect of reactive oxygen species and the activity of antioxidant systems on human semen; association with male infertility. Int J Androl 1997;20:255–264.
  • Zini A, Garrels K, Phang D. Antioxidant activity in the semen of fertile and infertile men. Urology 2000;55: 922–926.
  • Twigg J, Fulton N, Gomez E, Irvine DS, Aitken RJ. Analysis of the impact of intracellular reactive oxygen species generation on the structural and functional integrity of human spermatozoa: lipid peroxidation, DNA fragmentation and effectiveness of antioxidants. Hum Reprod 1998;13:1429–1436.
  • Giannattasio A, De Rosa M, Smeraglia R, Zarrilli S, Cimmino A, Di Rosario B, et al. Glutathione peroxidase (GPx) activity in seminal plasma of healthy and infertile males. J Endocrinol Invest 2002;25:983–986.
  • Garrido N, Meseguer M, Alvarez J, Simon C, Pellicer A, Remohi J. Relationship among standard semen parameters, glutathione peroxidase/glutathione reductase activity, and mRNA expression and reduced glutathione content in ejaculated spermatozoa from fertile and infertile men. Fertil Steril 2004;82:1059–1066.
  • de Lamirande E, Gagnon C. Impact of reactive oxygen species on spermatozoa: a balancing act between beneficial and detrimental effects. Hum Reprod 1995;10:15–21.
  • Aitken RJ, Clarkson JS. Cellular basis of defective sperm function and its association with the genesis of reactive oxygen species by human spermatozoa. J Reprod Fertil 1987;81: 459–469.
  • Alvarez JG, Storey BT. Differential incorporation of fatty acids into and peroxidative loss of fatty acids from phospholipids of human spermatozoa. Mol Reprod Dev 1995;42: 334–346.
  • Iwasaki A, Gagnon C. Formation of reactive oxygen species in spermatozoa of infertile patients. Fertil Steril 1992;57: 409–416.
  • Hellstrom WJG, Bell M, Wang R, Sikka SC. Effect of sodium nitroprusside on sperm motility, viability and lipid peroxidation. Fertil Steril 1994;61:1117–1122.
  • Falzone N, Huyser C, Fourie F, Toivo T, Leszczynski D, Franken D. In vitro effect of pulsed 900 MHz GSM radiation on mitochondrial membrane potential and motility of human spermatozoa. Bioelectromagnetics 2008;29:268–276.
  • Hu PY, Chu XL, Li JY, Yang D, He P. Effect of microwave contraception on human serum testosterone and luteinizing hormone. Shengzhi Yu Biyun 1985;5:32–34.
  • Adams ML, Meyer ER, Sewing BN, Cicero TJ. Effects of nitric oxide-related agents on rat testicular function. J Pharmacol Exp Ther 1994;269:230–237.
  • Punta KD, Charreau EH, Pignataro OP. Nitric oxide inhibits Leydig cell steroidogenesis. Endocrinology 1996; 137:5337–5343.
  • Weissman BA, Niu E, Ge R, Sottas CM, Holmes M, Hutson JC, Hardy MP. Paracrine modulation of androgen synthesis in rat leydig cells by nitric oxide. J Androl 2005; 26:369–378.
  • Singh VP, Chaturvedi CM. Correlation of Nitric oxide and testicular activity in laboratory mouse, Mus musculus. IJIRSET 2013;2:721–729.
  • Banerjee A, Anjum S, Verma R, Krishna A. Alteration in expression of estrogen receptor isoforms alpha and beta, and aromatase in the testes and its relation with changes in nitric oxide during aging in mice. Steroids 2012;77:609–620.

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