Updated Protocols and Comments on the Purification without Use of Activating Ligands of the Coupling Proteins N_s and N_i of the Hormone Sensitive Adenylyl Cyclase

References

• Londos C., Cooper D. M. F., Rodbell M. Receptor-mediated stimulation and inhibition of adenylate cyclase: The fat cell as a model system. Adv. Cyclic Nucl. Res. 1981; 14: 163–170
   PubMedGoogle Scholar
• Jakobs K. H., Aktories K., Schultz G. Inhibition of adenylate cyclase by hormones and neurotransmitters. Adv. Cyclic Nucl. Res. 1981; 14: 173–187
   PubMedGoogle Scholar
• Ross E. M., Howlett A. C., Ferguson K. M., Gilman A. G. Reconstitution of hormone-sensitive adenylate cyclase activity with resolved components of the enzyme. J. Biol. Chem. 1978; 253: 6401–6412
   PubMed Web of Science ®Google Scholar
• Pfeuffer T. Guanine nucleotide-controlled interactions between components of adenylate cyclase. FEBS Letters 1979; 101: 85–89
   PubMed Web of Science ®Google Scholar
• Rodbell M. The role of hormone receptors and GTP-regulatory proteins in membrane transduction. Nature 1980; 284: 17–22
   PubMed Web of Science ®Google Scholar
• Cooper D. M.F. Bimodal regulation of adenylate cyclase. FEBS Letters 1982; 138: 157–163
   PubMed Web of Science ®Google Scholar
• Hunzicker-Dunn M., Birnbaumer L. The involvement of adenylyl cyclase and cyclic AMP-dependent protein kinases in luteinizing hormone action. Gonadotropin Receptors and Gonadotropin Actions, M. Ascoli. CRC Press, Miami, In Press
   Google Scholar
• Hildebrandt J. D., Codina J., Risinger R., Birnbaumer L. Identification of a gamma subunit associated with the adenylyl cyclase regulatory proteins Ns and Ni. J. Biol. Chem 1984; 259: 2039–2042
   PubMedGoogle Scholar
• Iyengar R., Birnbaumer L. Hysteretic activation of adenylyl cyclases. I. Effect of Mg ion on the rate of activation by guannine nucleotides and fluoride. J. Biol. Chem. 1981; 256: 11036–11041
   PubMedGoogle Scholar
• Hildebrandt J. D., Birnbaumer L. Inhibitory regulation of adenylyl cyclase in the absence of stimulatory regulation. Requirements and kinetics of guanine nucleotide induced inhibition of the cyc− S49 adenylyl cyclase. J. Biol. Chem. 1983; 258: 13141–13147
   PubMed Web of Science ®Google Scholar
• Howlett A. C., Gilman A. G. Hydrodynamic properties of the regulatory component of adenylate cyclase. J. Biol. Chem 1980; 255: 2861–2866
   PubMed Web of Science ®Google Scholar
• Codina J., Hildebrandt J. D., Iyengar R., Birnbaumer L., Sekura R. D., Manclark C. R. Pertussis toxin substrate, the putative Ni of adenylyl cyclases, is an alpha/beta heterodimer regulated by guanine nucleotide and magnesium. Proc. Natl. Acad. Sci. U.S.A. 1983; 80: 4276–4280
   PubMed Web of Science ®Google Scholar
• Sternweis P. C., Northup J. K., Smigel M. D., Gilman A. G. The regulatory component of adenylate cyclase. Purification and properties. J. Biol. Chem. 1981; 256: 11517–11526
   PubMed Web of Science ®Google Scholar
• Northup J. K., Sternweis P. C., Gilman A. G. The subunits of the stimulatory regulatory component of adenylate cyclase. Resolution, activity, and properties of the 35,000 Dalton (.) subunit. J. Biol. Chem. 1983; 258: 11361–11368
   PubMed Web of Science ®Google Scholar
• Northup J. K., Smigel M. D., Sternweis P. C., Gilman A. G. The subunits of the stimulatory regulatory component of adenylate cyclase. Resolution, activity, and properties of the 45,000-Dalton (.) subunit. J. Biol. Chem. 1983; 258: 11369–11376
   PubMed Web of Science ®Google Scholar
• Codina J., Hildebrandt J. D., Birnbaumer L., Sekura R. D. Effects of guanine nucleotides and Mg on human erythrocyte Ni and Ns the regulatory components of adenylyl cyclase. J. Biol. Chem. 1984, Submitted
   Google Scholar
• Brandt D. R., Asano T., Pedersen S. E., Ross E. T. Reconstitution of catecholamine-stimulated guanosine-triphosphatase activity. Biochemistry 1983; 22: 4357–4362
   PubMed Web of Science ®Google Scholar
• Sunyer T., Codina J., Birnbaumer L. GTPase properties of Ni the inhibitory regulatory component of adenylyl cyclase. J. Biol. Chem. 1984, Submitted
   PubMedGoogle Scholar
• Cerione R. A., Codina J., Benovic J. L., Lefkowitz R. J., Birnbaumer L., Caron M. C. Reconstitution of pure components of a hormone responsive adenylate cyclase system: Interactions of the beta-adrenergic receptor and the stimulatory nucleotide binding protein (Ns). Biochemistry 1984, In Press
   PubMedGoogle Scholar
• Northup J. K., Sternweis P. C., Smigel M. D., Schleifer L. S., Ross E. M., Gilman A. G. Purification of the regulatory component of adenylate cyclase. Proc. Natl. Acad. Sci. U.S.A. 1980; 77: 6516–6520
   PubMedGoogle Scholar
• Pfeuffer T., Gaugler B., Metzger H. Isolation of homologous and heterologous complexes between catalytic and regulatory components of adenylate cyclase by forskolin-sepharose. FEBS Letters 1983; 164: 154–160
   PubMedGoogle Scholar
• Bokoch G. M., Katada T., Northup J. K., Hewlett E. L., Gilman A. G. Identification of the predominant substrate for ADP-ribosylation by islet activating protein. J. Biol. Chem. 1983; 258: 2072–2075
   PubMed Web of Science ®Google Scholar
• Manning D. R., Gilman A. G. The regulatory components of adenylate cyclase and transducin. A family of structurally homologous guanine nucleotide-binding proteins. J. Biol. Chem. 1983; 258: 7059–7063
   PubMed Web of Science ®Google Scholar
• Codina J., Hildebrandt J. D., Sekura R. D., Birnbaumer M., Bryan J., Manclark C. R., Iyengar R., Birnbaumer L. Ns and Ni the stimulatory and inhibitory regulatory components of adenylyl cyclases. Purification of the human erythrocyte proteins without the use of activating regulatory ligands. J. Biol. Chem. 1984, In Press
   Google Scholar
• Walseth T. F., Johnson R. A. The enzymatic preparation of [alpha-32P] nucleotide triphosphates, cyclic [32P]AMP and cyclic [32P]GMP. Biochim. Biophys. Acta 1979; 562: 11–31
   PubMedGoogle Scholar
• Birnbaumer L., Torres H. N., Flawia M. M., Fricke R. F. Improved methods for determination of guanylyl cyclase activity and synthesis of [alpha-32P]GTP. Anal Biochem. 1979; 93: 124–133
   PubMedGoogle Scholar
• Cassel D., Pfeuffer T. Mechanism of cholera toxin action: covalent modification of the guanyl nucleotide-binding protein of the adenylate cyclase system. Proc. Natl. Acad. Sci. U.S.A. 1978; 75: 2669–2673
   PubMed Web of Science ®Google Scholar
• Ross E. M., Maguire M. E., Sturgill T. W., Biltonen R. L., Gilman A. G. Relationship between the beta-adrenergic receptor and adenylate cyclase. Studies of ligand binding and enzyme activity in purified membranes of S49 lymphoma cells. J. Biol. Chem. 1977; 252: 5761–5775
   PubMed Web of Science ®Google Scholar
• Coffino P., Bourne H. R., Tomkins G. M. Somatic genetic analysis of cAMP action: selection of unresponsive mutants. J. Cell. Physiol. 1975; 85: 603–610
   PubMed Web of Science ®Google Scholar
• Laemmli U. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970; 227: 680–685
   PubMed Web of Science ®Google Scholar
• Poehlig H. M., Neuhoff V. Visualization of protein with a silver “stain”: a critical analysis. Electrophoresis 1981; 2: 141–147
   Google Scholar
• Wray G. W., Boulikas T., Wray V. P., Hancock R. Silver staining of proteins in polyacrylamide gels. Anal. Biochem. 1981; 118: 197–203
   PubMed Web of Science ®Google Scholar
• Birnbaumer L., Yang P.-Ch., Hunzicker-Dunn M., Bockaert J., Duran J. M. Adenylyl cyclase activities in ovarian tissues. I. Homogenization and conditions of assay in Graafian follicles and corporal lutea of rabbits, rats, and pigs, regulation by ATP and some comparative properties. Endocrinology 1976; 99: 163–184
   PubMed Web of Science ®Google Scholar
• Bockaert J., Hunzicker-Dunn M., Birnbaumer L. Hormone-stimulated desensitization of hormone-dependent adenylyl cyclase. Dual action of luteinizing hormone on pig Graafian follicle membranes. J. Biol. Chem. 1976; 251: 2653–02663
   PubMed Web of Science ®Google Scholar
• Salomon Y., Londos C., Rodbell M. A highly sensitive adenylate cyclase assay. Anal. Biochem. 1974; 58: 541–548
   PubMed Web of Science ®Google Scholar