Evaluation of four methods for processing human cord blood and subsequent study of the expansion of progenitor stem cells isolated using the best method

References

• Broxmeyer HE, Douglas GW, Hangoc G, Cooper S, Bard J, English D . Human umbilical cord blood as a potential source of transplantation hematopoietic stem/progenitor cells. Proc Natl Acad Sci USA 1989;86:3828–32.
   PubMed Web of Science ®Google Scholar
• Gluckman E, Broxmeyer HE, Auerbach AD, Friedman HS, Douglas GW, Devergie A, . Hematopoietic reconstitution in a patient with Fanconi's anemia by means of umbilical-cord blood from a HLA-identical sibling. N Engl J Med 1989;321:1174–8.
   PubMed Web of Science ®Google Scholar
• Orlic D, Kajstura J, Chimenti S, Jakoniuk I, Anderson SM, Li B, . Bone marrow cells regenerate infarcted myocardium. Nature 2001;410:701–5.
   PubMed Web of Science ®Google Scholar
• Leor J, Guetta E, Feinberg MS, Galski H, Bar I, Holbova R, . Human umbilical cord blood-derived CD133+ cells enhance function and repair of the infracted myocardium. Stem Cells 2006;24:772–80.
   PubMed Web of Science ®Google Scholar
• Lagasse E, Connors H, Al-Dhalimy M, Reitsma M, Dohse M, Osborne L, . Purified hematopoietic stem cells can differentiate into hepatocytes in vivo. Nat Med 2000;6:1229–34.
   PubMed Web of Science ®Google Scholar
• Almeida-Porada G, Porada CD, Chamberlain J, Torabi A, Zanjani ED. Formation of human hepatocytes by human hematopoietic stem cells in sheep. Blood 2004;104:2582– 90.
   PubMed Web of Science ®Google Scholar
• Ianus A, Holz GG, Theise ND, Hussain MA. In vivo derivation of glucose-competent pancreatic endocrine cells from bone marrow without evidence of cell fusion. J Clin Invest 2003;111:799–801.
   PubMedGoogle Scholar
• Wang X, Ge S, Gonzalez I, McNamara G, Rountree B, Xi KK, . Formation of pancreatic duct epithelium from bone marrow during neonatal development. Stem Cells 2006;24:307– 14.
   PubMedGoogle Scholar
• Rocha V, Wagner JE Jr , Sobocinkski KA, Klein JP, Zhang MJ, Horowitz MM, . Graft-versus host disease in children who have received a cord blood or bone marrow transplant from an HLA-identical sibling. N Engl J Med 2000;342:1846–54.
   PubMed Web of Science ®Google Scholar
• Hows JM, Bradley BA, Marsh JCW, Luft T, Coutinho L, Testa NG, . Growth of human umbilical-cord blood in longterm haemopoietic culture. Lancet 1992;340:73– 6.
   PubMed Web of Science ®Google Scholar
• Brandt JE, Bhalla K, Hoffman R. Effects of interleukin-3 and c-kit ligand on the survival of various classes of human hematopoietic progenitor cells. Blood 1994;83:1507–14.
   PubMed Web of Science ®Google Scholar
• Rubinstein P, Carrier C, Scaradavou A, Kurtzberg J, Adamson J, Migliaccio AR, . Outcomes of 562 recipients of placental-blood transplant from unrelated donors. N Engl J Med1998;339:1565–77.
   PubMed Web of Science ®Google Scholar
• Gluckman E, Rocha V, Boyer-Chammard A, Locatelli F, Arcese W, Pasquini R, . Outcome of cord-blood transplantation from related and unrelated donors. N Engl J Med 1997; 337: 373–81.
   PubMedGoogle Scholar
• Newton I, Charbord P, Schaal JP, Herve P. Towards cord blood banking: density-separation and cryopreservation of cord blood progenitors. Exp Hematol1993;21:671–4.
   PubMed Web of Science ®Google Scholar
• Regidor C, Posada M, Monteagudo D, Garaulet C, Somolinos N, Forés R, . Umbilical cord blood banking for unrelated transplantation: evaluation of cell separation and storage methods. Exp Hematol 1999;27:380–5.
   PubMedGoogle Scholar
• Rubinstein P, Dobrila L, Rosenfield RE, Adamson JW, Migliaccio G, Migliaccio AR, . Processing and cryo-preservation of placental/umbilical cord blood for unrelated bone marrow transplantation. Proc Natl Acad Sci USA 1995;292:10119–22.
   Google Scholar
• Bertolini F, Battaglia M, Zibera C, Baroni G, Soro V, Perotti C, . A new method for placental/cord blood processing in the collection bag. I. Analysis of factors involved in red blood cell removal. Bone Marrow Transplant 1996;18:783–6.
   Google Scholar
• Adorno G, Bruno A, Caravita T, Venditti A, Ballatore G, Santinelli S, . Red blood cell depletion of cord blood using hydroxyethyl starch double sedimentation: analysis of 40 cases. Clin Lab Haematol 1998;20:341–4.
   PubMedGoogle Scholar
• Perutelli P, Catellani S, Scarso L, Cornaglia-Ferraris P, Dini G. Processing of human cord blood by three different procedures for red blood cell depletion and mononuclear cell recovery. Vox Sang 1999;76:237–40.
   PubMedGoogle Scholar
• SchwingerW, Benesch M, Lackner H, Kerbl R, Walcher M, Urban C. Comparison of different methods for separation and ex vivo expansion of cord blood progenitor cells. Ann Hematol 1999;78:364–70.
   PubMedGoogle Scholar
• TokushmiaY, Sasayama N, Takahashi TA. Repopulating activities of human cord blood cells separated by a stem cell collection filter in NOD/SCID mice: a comparative study of filter method and HES method. Transfusion 2001;41:1014–19.
   PubMedGoogle Scholar
• Aoki M, Yasutake M, MuroharaT. Derivation of functional endothelial progenitor cells from human umbilical cord blood mononuclear cells isolated by a novel cell filtration device. Stem Cells 2004;22:994–1002.
   PubMedGoogle Scholar
• Kurtzberg J, Laughlin M, Graham ML, Smith C, Olson JF, Halperin EC, . Placental blood as a source of hematopoietic stem cells for transplantation into unrelated recipients. N Engl J Med 1996;335:157–66.
   PubMed Web of Science ®Google Scholar
• Laughlin MJ, Eapen M, Rubinstein P, Wagner JE, Zhang MJ, Champlin RE, . Outcomes after transplantation of cord blood and bone marrow from unrelated donors in adults with leukemia. N Engl J Med 2004;351:2265–75.
   PubMed Web of Science ®Google Scholar
• Reboredo NM, Diaz A, Castro A, Villaescusa RG. Collection, processing and cryopreservation of umbilical cord blood for unrelated transplantation. Bone Marrow Transplantation 2000;26:1263–70.
   PubMedGoogle Scholar
• Eichler H, Richter E, Leveringhaus A, Zieger W, Kerowgan M, Goldmann SF, . The Mannheim cord blood project: experience in collection and processing of the first 880 banked unrelated cord blood transplants. Infusion Ther Transfusion Med 1999; 80: 101–5.
   Google Scholar
• Dhot PS, Sirohi D, Swamy GLN. Collection, separation, enumeration and cryopreservation of umbilical cord blood hematopoietic stem cells: an experimental study. MJAFI 2003;59:298–301.
   Google Scholar
• Kekarainen T, Mannelin S, Laine J, Jaatinen T. Optimization of immunomagnetic separation for cord blood-derived hematopoietic cells. BMC Cell Biol 2006;7:30–41.
   PubMedGoogle Scholar
• Verstegen MM, van Hennik PB, Terpstra W, van den Bos C, Wielenga JJ, van Rooijen N, . Transplantation of human umbilical cord blood cells in macrophage-depleted SCID mice: evidence for accessory cell involvement in expansion of immature CD34+CD38 cells. Blood 1998;91:1966– 76.
   PubMed Web of Science ®Google Scholar
• Wagner JE, Barker JN, Defor T, Baker KS, Blazar BR, Eide C, . Transplantation of unrelated donor umbilical cord blood in 102 patients with malignant and non-malignant diseases: influence of CD34 cell dose and HLA disparity on treatment-related mortality and survival. Blood 2002;100:1611–18.
   PubMed Web of Science ®Google Scholar
• Summers YJ, heyworth CM, de Wynter EA, Hart CA, Chang J, Testa NG. AC133+ G0 cells from cord blood show a high incidence of long-term culture-initiating cells and a capacity for more than 100 million-fold amplification of colony-forming cells in vitro. Stem Cells 2004;22:704–15.
   PubMed Web of Science ®Google Scholar
• Summers YJ, heyworth CM, de Wynter EA, Chang J, Testa NG. Cord blood G(0) CD34+ cells have a thousand-fold higher capacity for generating progenitors in vitro than G(1) CD34+ cells. Stem Cells 2001;19:505– 13.
   PubMed Web of Science ®Google Scholar
• Dravid G, Rao SG, Ex vivo expansion of stem cells from umbilical cord blood: expression of cell adhesion molecules. Stem Cells 2002;20:183–9.
   PubMed Web of Science ®Google Scholar
• Kawano Y, Kobune M, Chiba H, Nakamura K, Takimoto R, Takada K, . Ex vivo expansion of G-CSF-mobilized peripheral blood CD133+ progenitor cells on co-culture with human stromal cells. Exp Hematol 2006;34:150–8.
   PubMedGoogle Scholar
• van Hensbergen Y, Schipper LF, Brand A, Slot MC, Welling M, Nauta AJ, . Ex vivo culture of human CD34+ cord blood cells with thrombopoietin (TPO) accelerates platelet engraftment in a NOD/SCID mouse model. Exp Hematol 2006;34:943–50.
   PubMedGoogle Scholar
• Barker JN, Krepski TP, DeFor TE, Davies SM, Wagner JE, Weisdorf DJ. Searching for unrelated donor hematopoietic stem cells: availability and speed of umbilical cord blood versus bone marrow. Biol Blood Marrow Transplant 2002: 8:25760.
   PubMed Web of Science ®Google Scholar
• Gluckman E, Rocha V, Chevret S, Results of unrelated umbilical cord blood transplant. Transfus Clin Biol 2001;8:146–54.
   PubMed Web of Science ®Google Scholar
• Pecora AL, Stiff P, Jennis A, Goldberg S, Rosenbluth R, Price P, . Prompt and durable engraftment in two older adult patients with high risk chronic myelogenous leukemia (CML) using ex vivo expanded and unmanipulated unrelated umbilical cord. Bone Marrow Transplantation 2000;25:797–9.
   PubMed Web of Science ®Google Scholar