Optimization and validation of a robust human T-cell culture method for monitoring phenotypic and polyfunctional antigen-specific CD4 and CD8 T-cell responses

References

• Simpson AJ, Caballero OL, Jungbluth A, Chen YT, Old LJ. Cancer/testis antigens, gametogenesis and cancer. Nat Rev Cancer 2005;5:615–25.
   PubMed Web of Science ®Google Scholar
• Gnjatic S, Nishikawa H, Jungbluth AA, Gure AO, Ritter G, Jager E, . NY-ESO-1: review of an immunogenic tumor antigen. Adv Cancer Res 2006;95:1–30.
   PubMed Web of Science ®Google Scholar
• Romero P, Valmori D, Pittet MJ, Zippelius A, Rimoldi D, Levy F, . Antigenicity and immunogenicity of Melan-A/MART-1 derived peptides as targets for tumor reactive CTL in human melanoma. Immunol Rev 2002;188:81–96.
   PubMed Web of Science ®Google Scholar
• Janetzki S, Panageas KS, Ben-Porat L, Boyer J, Britten CM, Clay TM, . Results and harmonization guidelines from two large-scale international Elispot proficiency panels conducted by the Cancer Vaccine Consortium (CVC/SVI). Cancer Immunol Immunother 2008;57:303–15.
   PubMed Web of Science ®Google Scholar
• Jager E, Nagata Y, Gnjatic S, Wada H, Stockert E, Karbach J, . Monitoring CD8 T cell responses to NY-ESO-1: correlation of humoral and cellular immune responses. Proc Natl Acad Sci USA 2000;97:4760–5.
   PubMed Web of Science ®Google Scholar
• Jager E, Karbach J, Gnjatic S, Neumann A, Bender A, Valmori D, . Recombinant vaccinia/fowlpox NY-ESO-1 vaccines induce both humoral and cellular NY-ESO-1-specific immune responses in cancer patients. Proc Natl Acad Sci USA 2006;103:14453–8.
   PubMed Web of Science ®Google Scholar
• Wolchok JD, Yuan J, Houghton AN, Gallardo HF, Rasalan TS, Wang J, . Safety and immunogenicity of tyrosinase DNA vaccines in patients with melanoma. Mol Ther 2007;15:2044–50.
   PubMed Web of Science ®Google Scholar
• Perales MA, Yuan J, Powel S, Gallardo HF, Rasalan TS, Gonzalez C, . Phase I/II study of GM-CSF DNA as an adjuvant for a multipeptide cancer vaccine in patients with advanced melanoma. Mol Ther 2008;16:2022–9.
   PubMed Web of Science ®Google Scholar
• Coulie PG, van der Bruggen P. T-cell responses of vaccinated cancer patients. Curr Opin Immunol 2003;15:131–7.
   PubMed Web of Science ®Google Scholar
• Zippelius A, Batard P, Rubio-Godoy V, Bioley G, Lienard D, Lejeune F, . Effector function of human tumor-specific CD8 T cells in melanoma lesions: a state of local functional tolerance. Cancer Res 2004;64:2865–73.
   PubMed Web of Science ®Google Scholar
• Sallusto F, Mackay CR, Lanzavecchia A. The role of chemokine receptors in primary, effector, and memory immune responses. Ann Rev Immunol 2000;18:593–620.
   PubMed Web of Science ®Google Scholar
• Sallusto F, Lenig D, Forster R, Lipp M, Lanzavecchia A. Two subsets of memory T lymphocytes with distinct homing potentials and effector functions. Nature 1999;401:708–12.
   PubMed Web of Science ®Google Scholar
• Sallusto F, Geginat J, Lanzavecchia A. Central memory and effector memory T cell subsets: function, generation, and maintenance. Ann Rev Immunol 2004;22:745–63.
   PubMed Web of Science ®Google Scholar
• Sauce D, Rufer N, Mercier P, Bodinier M, Remy-Martin J-P, Duperrier A, . Retrovirus-mediated gene transfer in polyclonal T cells results in lower apoptosis and enhanced ex vivo cell expansion of CMV-reactive CD8 T cells as compared with EBV-reactive CD8 T cells. Blood 2003;102:1241–8.
   Google Scholar
• Romero P, Zippelius A, Kurth I, Pittet MJ, Touvrey C, Iancu EM, . Four functionally distinct populations of human effector-memory CD8+ T lymphocytes. J Immunol 2007;178:4112–9.
   PubMedGoogle Scholar
• Seder RA, Darrah PA, Roederer M. T-cell quality in memory and protection: implications for vaccine design. Nature Rev 2008;8:247–58.
   Google Scholar
• Precopio ML, Betts MR, Parrino J, Price DA, Gostick E, Ambrozak DR, . Immunization with vaccinia virus induces polyfunctional and phenotypically distinctive CD8(+) T cell responses. J Exp Med 2007;204:1405–16.
   PubMed Web of Science ®Google Scholar
• Duvall MG, Precopio ML, Ambrozak DA, Jaye A, McMichael AJ, Whittle HC, . Polyfunctional T cell responses are a hallmark of HIV-2 infection. Eur J Immunol 2008;38:350–63.
   PubMed Web of Science ®Google Scholar
• Maecker HT, Rinfret A, D'Souza P, Darden J, Roig E, Landry C, . Standardization of cytokine flow cytometry assays. BMC Immunol 2005;6:13–30.
   PubMed Web of Science ®Google Scholar
• Horton H, Thomas EP, Stucky JA, Frank I, Moodie Z, Huang Y, . Optimization and validation of an 8-color intracellular cytokine staining (ICS) assay to quantify antigen-specific T cells induced by vaccination. J Immunol Meth 2007;323:39–54.
   PubMed Web of Science ®Google Scholar
• Chattopadhyay PK, Melenhorst JJ, Ladell K, Gostick E, Scheinberg P, Barrett AJ, . Techniques to improve the direct ex vivo detection of low frequency antigen-specific CD8+ T cells with peptide-major histocompatibility complex class I tetramers. Cytometry A 2008;73:1001–9.
   PubMedGoogle Scholar
• Wooldridge L, Lissina A, Cole DK, van den Berg HA, Price DA, Sewell AK. Tricks with tetramers: how to get the most from multimeric peptide-MHC. Immunology 2009;126:147–64.
   Google Scholar
• Jackson HM, Dimopoulos N, Chen Q, Luke T, Yee Tai T, Maraskovsky E, . A robust human T-cell culture method suitable for monitoring CD8+ and CD4+ T-cell responses from cancer clinical trial samples. J Immunol Meth 2004;291:51–62.
   PubMedGoogle Scholar
• Yuan J, Gallardo HF, Rasalan T, Ranganathan R, Wang J, Zhang Y, . In vitro expansion of Ag-specific T cells by HLA-A*0201-transfected K562 cells for immune monitoring. Cytotherapy 2006;8:498–508.
   Web of Science ®Google Scholar
• Foster AE, Leen AM, Lee T, Okamura T, Lu A, Vera J, . Autologous designer antigen-presenting cells by gene modification of T lymphocyte blasts with IL-7 and IL-12. J Immunother 2007;30:506–16.
   PubMed Web of Science ®Google Scholar
• Quintarelli C, Dotti G, De Angelis B, Hoyos V, Mims M, Luciano L, . Cytotoxic T lymphocytes directed to the preferentially expressed antigen of melanoma (PRAME) target chronic myeloid leukemia. Blood 2008;112:1876–85.
   PubMed Web of Science ®Google Scholar
• Melenhorst JJ, Solomon SR, Shenoy A, Hensel NF, McCoy JPJr, Keyvanfar K, . Robust expansion of viral antigen-specific CD4+ and CD8+ T cells for adoptive T cell therapy using gene-modified activated T cells as antigen presenting cells. J Immunother 2006;29:436–43. discussion 365–6
   PubMed Web of Science ®Google Scholar
• Betts MR, Brenchley JM, Price DA, De Rosa SC, Douek DC, Roederer M, . Sensitive and viable identification of antigen-specific CD8+ T cells by a flow cytometric assay for degranulation. J Immunol Meth 2003;281:65–78.
   PubMed Web of Science ®Google Scholar
• Yuan J, Gnjatic S, Li H, Powel S, Gallardo HF, Ritter E, . CTLA-4 blockade enhances polyfunctional NY-ESO-1 specific T cell responses in metastatic melanoma patients with clinical benefit. Proc Natl Acad Sci USA 2008;105:20410–5.
   PubMed Web of Science ®Google Scholar