756
Views
1
CrossRef citations to date
0
Altmetric
Research Article

Analysis of enzyme activity regulation by non-denaturing electrophoresis and application of this regulation for enzyme reactor production

&
Pages 894-899 | Received 06 Apr 2012, Accepted 10 May 2012, Published online: 18 Jul 2012

References

  • Shimazaki Y, Sugawara Y, Ohtsuka Y, Manabe T. Analysis of the activity and identification of enzymes after separation of cytosol proteins in mouse liver by microscale nondenaturing two-dimensional electrophoresis. Proteomics 2003;3:2002–2007.
  • Shimazaki Y, Sugawara Y, Manabe T. Nondenaturing two-dimensional electrophoresis enzyme profile involving activity and sequence structure of cytosol proteins from mouse liver. Proteomics 2004;4:1406–1411.
  • Wong C, Rodríguez-Páez L, Nogueda B, Pérez A, Baeza I. Selective inhibition of the sperm-specific lactate dehydrogenase isozyme-C4 by N-isopropyl oxamate. Biochim Biophys Acta 1997;1343:16–22.
  • Bencharit S, Morton CL, Hyatt JL, Kuhn P, Danks MK, Potter PM et al. Crystal structure of human carboxylesterase 1 complexed with the Alzheimer’s drug tacrine: from binding promiscuity to selective inhibition. Chem Biol 2003;10:341–349.
  • Shimazaki Y, Shimizu K, Masaoka S. Enzyme separation and isozyme heterogeneity analysis using non-denaturing two-dimensional electrophoresis. Talanta 2010;82:1063–1067.
  • Ishiyama M, Shiga M, Sasamoto K, Mizoguchi, M, He PG. A new sulfonated tetrazolium salt that products a highly water-soluble formazan dye. Chem Pharm Bull 1993;41:1118–1122.
  • Ishiyama M, Miyazono Y, Sasamoto K, Ohkura Y, Ueno K. A highly water-soluble disulfonated tetrazolium salt as a chromogenic indicator for NADH as well as cell viability. Talanta 1997;44:1299–1305.
  • Johnstone KJ, Ashford AE. A simultaneous-coupling azo dye method for the quantitative assay of esterase using α-naphthy acetate as a substrate. Histochem J 1980;12:221–234.
  • Shimazaki Y, Kuroda T. Production of enzyme reactors after separation by non-denaturing two-dimensional electrophoresis and immobilization on membrane. Biotechnol Lett 2009;31:1545–1549.
  • Shimazaki Y, Sakikawa T. Determination of malic Acid using a malate dehydrogenase reactor after purification and immobilization in non-denaturing conditions and staining with ponceau S. Protein Pept Lett 2010;17:1048–1052.
  • Yu Y, Deck JA, Hunsaker LA, Deck LM, Royer RE, Goldberg E et al. Selective active site inhibitors of human lactate dehydrogenases A4, B4, and C4. Biochem Pharmacol 2001;62:81–89.
  • Zhu B, Strada SJ. The novel functions of cGMP-specific phosphodiesterase 5 and its inhibitors in carcinoma cells and pulmonary/cardiovascular vessels. Curr Top Med Chem 2007;7:437–454.
  • Granchi C, Bertini S, Macchia M, Minutolo F. Inhibitors of lactate dehydrogenase isoforms and their therapeutic potentials. Curr Med Chem 2010;17:672–697.
  • Klose J, Spielmann H. Gel isoelectric focusing of mouse lactate dehydrogenase: heterogeneity of the isoenzymes A4 and X4. Biochem Genet 1975;13:707–720.
  • Lessard F, Dion R. Analysis for α-amylase isoenzymes by automated isoelectric focusing. Clin Chem 1989;35:2116–2118.
  • Wagner L, Wermann M, Rosche F, Rahfeld JU, Hoffmann T, Demuth HU. Isolation of dipeptidyl peptidase IV (DP 4) isoforms from porcine kidney by preparative isoelectric focusing to improve crystallization. Biol Chem 2011;392:665–677.
  • Ropchan JR, Barrio JR. Enzymatic synthesis of [1-11C]pyruvic acid, L-[1-11C]lactic acid and L-[1-11C]alanine via DL-[1-11C]alanine. J Nucl Med 1984;25:887–892.
  • Winkler FK, D’Arcy A, Hunziker W. Structure of human pancreatic lipase. Nature 1990;343:771–774.
  • Ranaldi S, Belle V, Woudstra M, Rodriguez J, Guigliarelli B, Sturgis J et al. Lid opening and unfolding in human pancreatic lipase at low pH revealed by site-directed spin labeling EPR and FTIR spectroscopy. Biochemistry 2009;48:630–638.
  • Belle V, Fournel A, Woudstra M, Ranaldi S, Prieri F, Thomé V et al. Probing the opening of the pancreatic lipase lid using site-directed spin labeling and EPR spectroscopy. Biochemistry 2007;46:2205–2214.
  • Kee CY, Hassan M, Ramachandran KB. Studies on the kinetics of Isopropyl Palmitate synthesis in packed bed bioreactor using immobilized lipase. Artif Cells Blood Substit Immobil Biotechnol 1999;27:393–398.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.