Effect of Medium Components and Cultural Conditions on the Production of Polysaccharide by Porodisculus pendulus and Mycelium Growth

Abstract

The effect of medium components and cultural conditions was studied on the production of polysaccharide (pendulan) and growth of mycelia in Porodisculus pendulus JTS–3009. Glucose, mannose, fructose, cellobiose, sucrose, mannitol and various starches were found to be suitable carbon sources. Pendulan was produced and mycelia grew well on ammonium-N, nitrate-N and organic nitrogen. The optimal concentration of nitrogen was 0.03% for production of pendulan and 0.04% for growth of mycelia. The addition of suitable amounts of yeast extract aided pendulan production and mycelium growth. The optimal temperature for pendulan production was about 29°C and for mycelium growth, 25˜30°C. The optimal pH for pendulan production was 6˜8 and for mycelium growth, 5˜6.5. The seed culture was stable after storage at 5°C or at −20°C with the addition of 20˜30% (v/v) glycerin. The maximum yield of pendulan and mycelia was obtained at 90˜120 hr of fermentation.