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Analytical Letters Volume 6, 1973 - Issue 5
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ANALYTICAL BIOCHEMISTRY AND CLINICAL ANALYSIS

A Novel Histamiiuse Assay, the use of Aminooxyacftic Acid

David Kupfer Lederle Laboratories, Division of American Cyanamid Company, Pearl River, New York, 10965
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Henry G. Roscoe Lederle Laboratories, Division of American Cyanamid Company, Pearl River, New York, 10965
Pages 397-406 | Received 13 Mar 1973, Accepted 16 Apr 1973, Published online: 05 Dec 2006
 
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Abstract

This report describes the development of a rapid and extremely sensitive radioactive assay for histaminase activity. The assay is based on the reaction of aminooxyacetic acid with the primary histamine metabolite [imidazole acetaldehyde (IA)] in basic solution, followed by extraction of unreacted histamine. The radioactivity remaining in the aqueous phase due to IA and its potential metabolite (imidazole acetic acid) is determined.

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