References
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- For optimal results the pH of the resulting solution should exceed 11.5.
- It is essential to use saturated butanol as described under Reagents. Extraction with butanol which has not been saturated, substantially decreased the volume of the aqueous phase and erroneous results seemed to occur.
- Two butanol extractions were sufficient for removal of all of the unmetabolized histamine.
- Bray , G. A. 1960 . Anal. Biochem. , 1 : 279
- The use of commercially available phase combining systems, such as aquasol (New England Nuclear) or PCS (Amersham/Searle) would facilitate the counting of larger aliquots.