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ANALYTICAL BIOCHEMISTRY AND CLINICAL ANALYSIS

A Novel Histamiiuse Assay, the use of Aminooxyacftic Acid

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Pages 397-406 | Received 13 Mar 1973, Accepted 16 Apr 1973, Published online: 05 Dec 2006

References

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  • For optimal results the pH of the resulting solution should exceed 11.5.
  • It is essential to use saturated butanol as described under Reagents. Extraction with butanol which has not been saturated, substantially decreased the volume of the aqueous phase and erroneous results seemed to occur.
  • Two butanol extractions were sufficient for removal of all of the unmetabolized histamine.
  • Bray , G. A. 1960 . Anal. Biochem. , 1 : 279
  • The use of commercially available phase combining systems, such as aquasol (New England Nuclear) or PCS (Amersham/Searle) would facilitate the counting of larger aliquots.

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