Three new Pythium species from rice paddy fields

ABSTRACT

In an investigation of the oomyceteous flora in rice paddy fields of Fars Province, Iran, three new Pythium species were isolated and identified on the basis of morphological features and molecular phylogenetic characteristics. Their unique morphological traits, including sexual and asexual structural characteristics (i.e., sporangial type; oogonial type and ornamentations; type and the number of antheridia per oogonium; and oospore type), cardinal temperatures, and colony morphology on various media, separated them from other known species. Using nuclear and mitochondrial genes, each species formed discrete lineages in phylogenetic analyses based on Bayesian inference and maximum likelihood methods. This paper describes these three new Pythium species, P. heteroogonium, P. longipapillum, and P. oryzicollum, and compares them with their related taxa via morphological features and molecular characteristics. Pathogenicity tests revealed the ability of P. oryzicollum to cause pre- and post-emergence damping-off, seed rot, crown rot, and reduced growth rate on rice.

KEYWORDS:

• Morphology
• pathogenicity
• phylogeny
• Pythium
• taxonomy
• 3 new taxa

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ACKNOWLEDGMENTS

We would like to thank the late Prof. Walter Gams for his comments on species names and Dr. Arthur W. A. M. de Cock for his valuable comments on the paper.

Additional information

Funding

This study was funded by the Iran National Science Foundation (INSF; award number 96008191).