Publication Cover
Xenobiotica
the fate of foreign compounds in biological systems
Volume 51, 2021 - Issue 9
119
Views
1
CrossRef citations to date
0
Altmetric
Molecular Toxicology

Interactions between cadmium and zinc on gene expression pattern of differentiation markers in MC3T3-E1 cell line

, , ORCID Icon &
Pages 1038-1046 | Received 11 May 2021, Accepted 30 Jul 2021, Published online: 11 Aug 2021
 

Abstract

  1. We evaluated, in vitro, the interactions between cadmium (Cd) and zinc (Zn) during the proliferation and differentiation process using bone MC3T3-E1 cell line.

  2. Cells were treated with CdCl2 and/or ZnCl2 for 24 and 48h and 5µM CdCl2 was found as low cytotoxic dose and 25µM ZnCl2 as the best Zn treatment for cell proliferation. Gene expression of some bone markers (Runx2, collagen α1 (Colα1), osteocalcin (Oc), alkaline phosphatase (ALP) and bone sialoprotein (BSP)) was studied at 24, 48 and 72h.

  3. Treatment by CdCl2 depressed Runx2, Colα1, and BSP mRNA levels after 24h. Oc and ALP gene expression was found to be decreased after 72h.

  4. CdCl2 -exposure decreased ALP activity and Ca deposit in matrix. In concomitant treatment by CdCl2 and ZnCl2, gene expression of osteoblastic markers was found to be up-regulated (p<0, 05) compared to CdCl2 treated cells, ALP staining and mineralization were increased.

  5. Our results show that Zn could prevent Cd-induced toxicity on MC3T3-E1 cells, probably through the restoration of Runx2, col α1, BSP, ALP and Oc and gene expression inhibited by Cd.

Additional information

Funding

This research was supported by Inserm U1132 & USPC Paris-Diderot Department of rheumatology Hôpital Lariboisière and Ministry of Higher Education, Scientific Research and Technology of Tunisia. Sana Boughammoura received an international graduate research award from University of Monastir, Tunisia.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.