Optimization of enzyme-assisted extraction of ginsenoside Rb1 from Vietnamese Panax notoginseng (BURK.) F.H. Chen roots and anticancer activity examination of the extract

ABSTRACT

In this study, the conditions of ginsenoside Rb1 extraction from Panax notoginseng (Burk.) F.H. Chen (P. notoginseng) roots by enzyme-assisted method were investigated by using the central composite design (CCD). This design was applied to survey the effects of pH, temperature, and time on Rb1 content. The concentration of Rb1 was determined based on high-performance liquid chromatography (HPLC). The investigation of anticancer activities of P. notoginseng extract on breast carcinoma (MCF-7) cell lines and human lung carcinoma (Lu) showed that the extract had remarkable cytotoxic activity against these cancer cell lines.

KEYWORDS:

• Panax notoginseng (Burk.) F.H. Chen
• response surface methodology
• central composite design
• ginsenoside Rb1
• anticancer

Acknowledgements

We acknowledge the support of time and facilities from Ho Chi Minh City University of Technology (HCMUT), VNU-HCM for this study.