Deoxynivalenol impairs proliferation and induces apoptosis in primary murine osteoblasts

ABSTRACT

The effects of deoxynivalenol in different dose including 100, 200, 500, 1000, and 1500 µg/L on primary cranial osteoblasts from fetal mice were investigated. Fluorescence staining, flow cytometric measurement, 3-(4,5-dimethylthiazol 2-y1)-3,5-di-phenyltetrazolium bromide assay, quantitative PCR, and Western blot were used for the test. Mineralization and proliferation of osteoblasts decreased upon 100 µg/L and higher deoxynivalenol treatment and apoptosis of osteoblasts was increased upon 500, 1000, and 1500 µg/L deoxynivalenol treatment. Karyopyknosis, membrane breakage, and a decreased number of calcium nodes were also observed upon 500 µg/L deoxynivalenol treatment. The mRNA and protein levels of B-cell lymphoma-2-associated X protein were upregulated, B-cell lymphoma-2 protein downregulated with increasing concentrations of deoxynivalenol treatment and their ratio increased. Deoxynivalenol induces apoptosis of osteoblasts, suggesting a mechanism by which deoxynivalenol can affect murine skeletal development.

KEYWORDS:

• Deoxynivalenol
• osteoblasts
• apoptosis
• Bcl-2-associated X protein
• B-cell lymphoma-2

Acknowledgments

This work was supported by the National Foundation of Natural Science of China under Grant 81602455, the Science Foundation of Shandong Province, under Grant ZR2013HM033 and ZR2013HL060, the Colleges and Universities Technology Plan Item foundation of Shandong Province, under Grant J11LF12 and J17KA139, the Science and Technology Development Plan of Taian under Grant 2016NS1074, 2016NS1089 and 2016NS1134, and the Medicine Technology Plan Item Foundation of Taishan Medical University under Grant 2014GCC10 and 341680.

Disclosure statement

The authors report no conflicts of interest.

Additional information

Funding

This work was supported by the National Foundation of Natural Science of China [grant number 81602455]; the Science Foundation of Shandong Province [grant number ZR2013HM033], [grant number ZR2013HL060]; the Colleges and Universities Technology Plan Item foundation of Shandong Province [grant number J11LF12], [grant number J17KA139]; the Science and Technology Development Plan of Taian [grant number 2016NS1074], [grant number 2016NS1089], [grant number 2016NS1134]; the Medicine Technology Plan Item Foundation of Taishan Medical University [grant number 2014GCC10], [grant number 341680].