ABCG2 and SLCO1B1 gene polymorphisms in the Croatian population

Abstract

Background

Organic anion-transporting polypeptide 1B1 (OATP1B1) and the ATP-binding cassette subfamily G member 2, ABCG2, are important transporters involved in the transport of endogenous substrates and xenobiotics, including drugs. Genetic polymorphisms of these transporters have effect on transporter activity. There is significant interethnic variability in the frequency of allele variants.

Aim

To determined allele and genotype frequencies of ABCG2 and SLCO1B1 genes in Croatian populations of European descent.

Subjects and methods

A total of 905 subjects (482 women) were included. Genotyping for ABCG2 c.421C > A (rs2231142) and for SLCO1B1 c.521T > C (rs4149056), was performed by real-time polymerase chain reaction (PCR) using TaqMan^® DME Genotyping Assays.

Results

For ABCG2 c.421C > A, the frequency of CC, CA and AA genotypes was 81.4%, 17.8% and 0.8% respectively. The frequency of variant ABCG2 421 A allele was 9.7%. For SLCO1B1 c.521T > C, the frequency of TT, TC and CC genotypes was 61.7%, 34.8% and 3.5% respectively. The frequency of variant SLCO1B1 521 C allele was 20.9%.

Conclusion

The frequency of the ABCG2 and SLCO1B1 allelic variants and genotypes in the Croatian population is in accordance with other European populations. Pharmacogenetic analysis can serve to individualise drug therapy and minimise the risk of developing adverse drug reactions.

Keywords:

• Croatian population
• ABCG2
• SLCO1B1
• drug transporters
• gene polymorphism

Ethics approval

Study was approved by the Ethics committee of the University Hospital Centre Zagreb and Zagreb University School of Medicine (approval number 380-59-10106-20-111/125 and 02/21 AG). All procedures performed in the study were in accordance with the 1964 Declaration of Helsinki and its later amendments.

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

The research was partly funded by the grant from the Croatian Science Foundation for the Installation Research Projects [UIP-2020-02-8189]” Pharmacogenomics in prediction of cardiovascular drugs adverse reaction” (PGx-CardioDrug).