Low dose exposure of patulin and protective effect of epicatechin on blood cells in vitro

Abstract

In the present study, we aimed to assess antioxidant status in erythrocytes in vitro after patulin (PAT) and epicatechin exposure by measuring antioxidant enzymes (superoxide-dismutase – SOD, glutathione peroxidase – GPx and catalase – CAT) and parameters associated with oxidative stress (malondialdehyde – MDA and ROS). We also investigated the effect of PAT on viability and count of lymphocytes and lymphocyte subpopulations in rabbit blood in vitro. Whole blood of rabbits was used for analysis of antioxidant changes in rabbit erythrocytes after epicatechin and PAT treatment (separately or in combination, at concentrations of 0.2; 2; 20; 200 µg mL^–1 of epicatechin and 0.5; 5; 10 µg mL^–1 of PAT). Whole blood of rabbits was also used for analysis of count and viability of lymphocytes after PAT treatment at concentrations of 10; 25 and 50 µg mL^–1. Results from our experiment confirmed the ability of epicatechin to protect cells against oxidative stress and lipoperoxidation. Our findings indicate that mycotoxin PAT in low concentrations did not affect the activity of antioxidant enzymes in erythrocytes of rabbits significantly. Only slight non-significant changes in lymphocytes count after treatment with low doses of PAT in rabbit blood were observed.

Keywords:

• Blood cells
• patulin
• epicatechin
• antioxidant status
• cytometric analysis

Additional information

Funding

This work was financially supported by VEGA scientific grant VEGA 1/0760/15 and by the Slovak Research and Development Agency under the contract No. APVV-16-0289 and APVV-15-0543. This work was co-funded by European Community under project no. 26220220180: Building Research Centre “AgroBioTech”.