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Nucleosides and Nucleotides Volume 6, 1987 - Issue 1-2
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Preparative Syntheses of Guanylate-Rich Oligonucleotides Using the Phosphotriester Method in Solution

H. Schott Institut für Organische Chemie der Universität Tübingen Auf der Morgenstelle, 18 D-7400, Tübingen
,
R. Semmler Institut für Organische Chemie der Universität Tübingen Auf der Morgenstelle, 18 D-7400, Tübingen
&
K. Cloß Institut für Organische Chemie der Universität Tübingen Auf der Morgenstelle, 18 D-7400, Tübingen
Pages 407-408 | Published online: 05 Dec 2006
 
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Abstract

The guanylate-rich fragments: 150 mg d(G4T4), 180 mg d(T4G4), 350 mg d(T4G4T4), 30 mg d(G4T4G4), 85 mg d(T4G4T4G4)were synthesized using the triester method. By enzymatic ligation of aliquots the 36mer d(G4T4G4T4G4T4G4T4G4) is obtained.

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