Abstract
It is reported on solid-phase chemical degradation sequencing of lore than 50 synthetic DNA fragments containing various 5-substituted pyrimidines including uracil (U), 5-fluorouracil (F5U), 5-bromouracil (Br5U), α and β anomers of 5-propyluracil (α-prop5U and B-prop5U) and 5-methylcytosine (a5C), Characteristic sequence patterns of oligonucleotides containing these base analogues are presented and their modification behaviour discussed