Enhanced Antisense Effects Resulting from an Improved Streptolysin-O Protocol for Oligodeoxynucleotide Delivery into Human Leukaemia Cells

R. V. Giles Department of Biochemistry, Liverpool University, P.O. Box 147, Liverpool, Merseyside, L69 3BX, UK

J. Grzybowski Department of Biochemistry, Liverpool University, P.O. Box 147, Liverpool, Merseyside, L69 3BX, UK

D. G. Spiller Department of Biochemistry, Liverpool University, P.O. Box 147, Liverpool, Merseyside, L69 3BX, UK

D. M. Tidd Department of Biochemistry, Liverpool University, P.O. Box 147, Liverpool, Merseyside, L69 3BX, UK

Abstract

An improved protocol for delivering oligodeoxynucleotides into the cytoplasm and nucleus of human cells in culture using streptolysin O is described. The new procedure permitted reduced target protein expression to result from antisense suppression of target mRNA levels.

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Enhanced Antisense Effects Resulting from an Improved Streptolysin-O Protocol for Oligodeoxynucleotide Delivery into Human Leukaemia Cells

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Enhanced Antisense Effects Resulting from an Improved Streptolysin-O Protocol for Oligodeoxynucleotide Delivery into Human Leukaemia Cells

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