Oligonucleotides Containing N⁷-Cyanoborane-2′-deoxyguanosine

Abstract

For synthesis of N⁷-cyanoborane-containing oligonucleotides, the 5′-DMT protecting group is not a suitable precursor because the boronated nucleoside is incompatible with DMT cations released during deprotection of the oligonucleotide. As an alternative to DMT, we have investigated use of the 5′-Fmoc protecting group. We found that the cyanoborane group is stable during synthesis and deprotection conditions used with Fmoc derivatives.

Notes

Unpublished results.